A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker

Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a “turn-on” bioluminescent readout of a C. difficile-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was “caged” with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was released and NanoLuc activity was restored, providing a visible readout. The bioluminescent sensor detected PPEP-1 concentrations as low as 10 nM. Sensor uncaging was achieved within minutes, and signal was captured using a digital camera. Importantly, the sensor was also functional at ambient temperature and compatible with fecal material, suggesting that it can be readily deployed in a variety of settings.

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Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min

ACS Central Science ◽

10.1021/acscentsci.0c01288 ◽

2021 ◽

Vol 7 (2) ◽

pp. 307-317

Author(s):

Jesus Rodriguez-Manzano ◽

Kenny Malpartida-Cardenas ◽

Nicolas Moser ◽

Ivana Pennisi ◽

Matthew Cavuto ◽

...

Keyword(s):

Rapid Detection ◽

Point Of Care ◽

Point Of Care System ◽

Care System

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A turn-on fluorescent sensing strategy for rapid detection of Flumequine in water environment using covalent-coordination functionalized MOFs

CrystEngComm ◽

10.1039/d1ce00668a ◽

2021 ◽

Author(s):

Shengnan Li ◽

Ying Li ◽

Bing Yan

Keyword(s):

Rapid Detection ◽

Water Environment ◽

Fluorescent Sensing ◽

Turn On ◽

High Output

With the high output and large use of antibiotics in the process of aquaculture, the pollution caused by antibiotics in water environment is becoming a thorny problem, and its ecological...

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A New Fluorescence Probe for Rapid Detection of Amyloid

10.26434/chemrxiv.6982895.v1 ◽

2018 ◽

Author(s):

Nidhi Gour ◽

Vivek Shinh Kshtriya ◽

Bharti Koshti ◽

Shradhey Gupta ◽

khashti Ballabh Joshi ◽

...

Keyword(s):

Rapid Detection ◽

Control Experiment ◽

Fluorescence Probe ◽

Photophysical Properties ◽

Cost Effective ◽

The Novel ◽

High Quantum Yield ◽

Turn On ◽

Heat Treated ◽

Amyloidogenic Peptides

We report efficient synthesis and photophysical properties of a novel fluorescent pyridothiazole based construct by a facile methodology and its application as a sensor for rapid, selective as well as sensitive detection of amyloid fibres. The novel compound has exceptionally high quantum yield and sensitivity. The fluorescence of pyridothiazole construct is completely quenched (Turn-OFF) upon addition of cupric ions and the fluorescence is recovered on addition of amyloidogenic peptides like Aβ42 (Turn-ON). The control experiment with native and heat treated BSA suggest that the new probe binds to beeta/β sheets present in amyloid. The new probe offers direct visualization of fluorescence ‘Turn-OFF’ ‘Turn-ON’ under long UV without the use of sophisticated instrumentation. Hence, a very simple, facile and cost-effective methodology for the rapid detection of amyloid is presented and it may in all possibility pave the way for the design of a better probe for sensing amyloids than conventional ThT.

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Two Phenanthroimidazole Turn-on Probes for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2021.120585 ◽

2021 ◽

pp. 120585

Author(s):

Zongcheng Wang ◽

Weikang Su ◽

Huihuang Zheng ◽

Shun Yang ◽

Tingting Yang ◽

...

Keyword(s):

Rapid Detection ◽

Living Cells ◽

Turn On

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The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

ACS Central Science ◽

10.1021/acscentsci.0c00855 ◽

2020 ◽

Vol 6 (11) ◽

pp. 2046-2052 ◽

Cited By ~ 5

Author(s):

Alexander N. Baker ◽

Sarah-Jane Richards ◽

Collette S. Guy ◽

Thomas R. Congdon ◽

Muhammad Hasan ◽

...

Keyword(s):

Rapid Detection ◽

Point Of Care ◽

Sialic Acids ◽

Lateral Flow ◽

Spike Protein ◽

Diagnostic Device

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A NIR fluorescence probe having significant fluorescence turn-on signal at 700 nm and large Stokes shift for rapid detection of HOCl in vivo

Talanta ◽

10.1016/j.talanta.2020.121768 ◽

2021 ◽

Vol 223 ◽

pp. 121768

Author(s):

Haiyan Zhang ◽

Xinyu Yin ◽

Jiaxin Hong ◽

Yingzhen Deng ◽

Guoqiang Feng

Keyword(s):

Rapid Detection ◽

Fluorescence Probe ◽

Stokes Shift ◽

Large Stokes Shift ◽

Turn On ◽

Nir Fluorescence ◽

Fluorescence Turn On

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A Turn‐On Fluorescence Probe for Rapid Detection of Hypochlorite in Living Cells and in Vitro

ChemistrySelect ◽

10.1002/slct.201901468 ◽

2019 ◽

Vol 4 (27) ◽

pp. 8021-8025 ◽

Cited By ~ 3

Author(s):

Ya Xie ◽

Lijia Haung ◽

Liqiang Yan ◽

Jianping Li

Keyword(s):

Rapid Detection ◽

Fluorescence Probe ◽

Living Cells ◽

Turn On

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Diagnosing heart failure with NT-proBNP point-of-care testing: lower costs and better outcomes. A decision analytic study

BJGP Open ◽

10.3399/bjgpopen18x101596 ◽

2018 ◽

Vol 2 (3) ◽

pp. bjgpopen18X101596 ◽

Cited By ~ 3

Author(s):

Christoffer Bugge ◽

Erik Magnus Sether ◽

Andreas Pahle ◽

Sigrun Halvorsen ◽

Ivar Sonbo Kristiansen

Keyword(s):

Heart Failure ◽

Clinical Diagnosis ◽

Point Of Care ◽

Adult Population ◽

Delayed Diagnosis ◽

Clinical Findings ◽

Sensitivity Analyses ◽

Tree Model ◽

Diagnostic Strategies ◽

Point Of Care Test

BackgroundAfflicting 1–2% of the adult population, heart failure (HF) is a condition with considerable morbidity and mortality. While echocardiography may be considered the gold standard diagnostic test, GPs have relied on symptoms and clinical findings in diagnosing the condition.AimThe aim of this study was to estimate 1-year health outcome and costs of three diagnostic strategies: 1) history and clinical findings ('clinical diagnosis'); 2) clinical diagnosis supplemented with NTproBNP point-of-care test ('POC test') in the GP’s surgery; or (3) in hospital laboratory ('hospital test').Design & settingA decision tree model was developed to simulate 1-year patient courses with each strategy in Norway.MethodSensitivity and specificity of clinical diagnosis (56% and 68%), and of N-terminal pro B-type natriuretic peptide test ([NT-proBNP] 90% and 65%), were based on published literature. The probabilities of referral to hospital were based on a survey of Norwegian GPs (n = 103). The costs were based on various Norwegian fee schedules. Sensitivity analyses were conducted to examine the uncertainty of the results.ResultsThe 1-year per person societal costs were €543, €505, and €607 for clinical diagnosis, POC test, and hospital test, respectively. Even though POC entails higher laboratory costs, the total primary care costs were lower because of fewer re-visits with the GP and less use of spirometry. While 38% of patients had a delayed diagnosis with clinical diagnosis, the proportions were 22% with both POC test and hospital test. Results were most sensitive to the probability of use of spirometry.ConclusionPOC testing results in earlier diagnosis and lower costs than the other diagnostic modalities.

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