scholarly journals Real-Time Monitoring of Doxorubicin Release from Hybrid Nanoporous Anodic Alumina Structures

Sensors ◽  
2021 ◽  
Vol 21 (23) ◽  
pp. 7819
Author(s):  
Pankaj Kapruwan ◽  
Josep Ferré-Borrull ◽  
Lluis F. Marsal
Keyword(s):  
Real Time ◽  
Hybrid Structure ◽  
Anodic Alumina ◽  
Absorption Region ◽  
Nanoporous Anodic Alumina ◽  
Pore Length ◽  
Average Reflectance ◽  
Constant Potential ◽  
Doxorubicin Release ◽  
Two Parameters

This work demonstrates an advanced approach to fabricate Hybrid nanoporous anodic alumina gradient-index filters (Hy-NAA-GIFs) through a heterogeneous anodization process combining sinusoidal current-density anodization and constant potential anodization. As a result, the hybrid structure obtained reveals a single photonic stopband (PSB), which falls within the absorption region of the drug molecule and the intensity of the spectrum that are far from such absorption range. The prepared structures were loaded with the doxorubicin (DOX) drug through the drop-casting method, which allows for evaluating the maximum reflectance of the relative height of the PSB with the average reflectance of the spectrum intensity. Thereafter, this property has been applied in a flow cell setup connected to a reflectance spectrophotometer where different drug-loaded samples were placed to study the behavior and kinetics of the drug release in real-time by varying two parameters, i.e., different pore length and flow rates. As such, obtained results were analyzed with a model that includes a sum of two inverted exponential decay functions with two different characteristic time releases. Overall, this study opens up several possibilities for the Hy-NAA-GIFs to study the drug kinetics from nanoporous structures.

scholarly journals Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 478 ◽  
Author(s):  
Laura Pol ◽  
Chris Eckstein ◽  
Laura Acosta ◽  
Elisabet Xifré-Pérez ◽  
Josep Ferré-Borrull ◽  
...  
Keyword(s):  
Surface Modification ◽  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Real Time Monitoring ◽  
Helpful Tool ◽  
Nanoporous Anodic Alumina ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Molecule Model

The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.

scholarly journals 1-D nanoporous anodic alumina rugate filters by means of small current variations for real-time sensing applications

2014 ◽  
Vol 9 (1) ◽  
pp. 315 ◽  
Author(s):  
Gerard Macias ◽  
Josep Ferré-Borrull ◽  
Josep Pallarès ◽  
Lluís F Marsal
Keyword(s):  
Real Time ◽  
Anodic Alumina ◽  
Small Current ◽  
Nanoporous Anodic Alumina ◽  
Sensing Applications

scholarly journals Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4543 ◽  
Author(s):  
Laura Pol ◽  
Laura Karen Acosta ◽  
Josep Ferré-Borrull ◽  
Lluis F. Marsal
Keyword(s):  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Chemical Modifications ◽  
Efficient Manner ◽  
Nanoporous Anodic Alumina ◽  
Chemically Modified ◽  
High Affinity ◽  
Interferometric Biosensor ◽  
Inner Pore

Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.

scholarly journals Optical Platform to Analyze a Model Drug-Loading and Releasing Profile Based on Nanoporous Anodic Alumina Gradient Index Filters

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 730
Author(s):  
Pankaj Kapruwan ◽  
Laura K. Acosta ◽  
Josep Ferré-Borrull ◽  
Lluis F. Marsal
Keyword(s):  
Real Time ◽  
Drug Loading ◽  
Anodic Alumina ◽  
Gradient Index ◽  
Layer By Layer ◽  
Nanoporous Anodic Alumina ◽  
Layer Deposition ◽  
Model Drug ◽  
Surface Modified ◽  
Cargo Molecule

In this work, a methodology that exploits the optical properties of the nanoporous anodic alumina gradient index filters (NAA-GIFs) has been developed and applied to evaluate in real time the release dynamics of a cargo molecule, acting as a model drug, filling the pores. NAA-GIFs with two photonic stopbands (PSBs) were prepared with one of its stop bands in the same absorption wavelength range of the cargo molecule, whereas the second stopband away from this absorption range. Numerical simulation and experiments confirm that the relative height of the high reflectance bands in the reflectance spectra of NAA-GIFs filled with the drug can be related to the relative amount of drug filling the pores. This property has been applied in a flow cell setup to measure in real-time the release dynamics of NAA-GIFs with the inner pore surface modified by layer-by-layer deposition of polyelectrolytes and loaded with the cargo molecule. The methodology developed in this work acts as a tool for the study of drug delivery from porous nanostructures.

Structural and Optical Nanoengineering of Nanoporous Anodic Alumina Rugate Filters for Real-Time and Label-Free Biosensing Applications

2014 ◽  
Vol 86 (3) ◽  
pp. 1837-1844 ◽  
Author(s):  
Tushar Kumeria ◽  
Mohammad Mahbubur Rahman ◽  
Abel Santos ◽  
Josep Ferré-Borrull ◽  
Lluís F. Marsal ◽  
...  
Keyword(s):  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Nanoporous Anodic Alumina

Photoswitchable Membranes Based on Peptide‐Modified Nanoporous Anodic Alumina: Toward Smart Membranes for On‐Demand Molecular Transport

2015 ◽  
Vol 27 (19) ◽  
pp. 3019-3024 ◽  
Author(s):  
Tushar Kumeria ◽  
Jingxian Yu ◽  
Mohammed Alsawat ◽  
Mahaveer D. Kurkuri ◽  
Abel Santos ◽  
...  
Keyword(s):  
Molecular Transport ◽  
Anodic Alumina ◽  
Nanoporous Anodic Alumina ◽  
On Demand

New 8-oxo-7,8-2´deoxyguanosine nanoporous anodic alumina aptasensor for colorectal cancer diagnosis in blood and urine

Nanoscale ◽  
2021 ◽  
Author(s):  
Luis Pla ◽  
Felix Sancenón ◽  
M.Carmen Martinez-Bisbal ◽  
Celia Banuls ◽  
Nuria Estañ ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Cancer Diagnosis ◽  
Anodic Alumina ◽  
Human Diseases ◽  
Nanoporous Anodic Alumina

Many important human diseases, and specially cancer, have been related to the overproduction of 8-oxo-7,8-dihydro-2´-deoxyguanosine (8-oxo-dG). This molecule is a product of oxidative stress processes over nucleophilic bases in DNA....

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