scholarly journals Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 75
Author(s):  
Aleksey Firsov ◽  
Alexander Pushin ◽  
Svetlana Motyleva ◽  
Svetlana Pigoleva ◽  
Lyubov Shaloiko ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Multidrug Resistant ◽  
Chrysanthemum Morifolium ◽  
Cytochrome P450 Reductase ◽  
Heterologous Biosynthesis ◽  
Transgenic Lines ◽  
Melting Analysis ◽  
Commercial Source ◽  
Chrysanthemum Morifolium Ramat ◽  
Artemisinin Biosynthesis

Artemisinin-based drugs are the most effective medicine against multidrug-resistant Plasmodium spp., the parasite that causes malaria. To this day, wormwood A. annua L. is the sole commercial source of artemisinin, where it is produced in minor amounts. The artemisinin yield depends on numerous poorly regulated agricultural factors and the genetic variability of this non-domesticated plant. This has aroused significant interest in the development of heterologous expression platforms for artemisinin production. Previously, we obtained lines of Chrysanthemum morifolium Ramat. (C. morifolium Ramat.), cvs. White Snowdon and Egyptianka, transformed with artemisinin biosynthesis genes. Here, we report the results of an analysis of artemisinin production in transgenic chrysanthemums. Transcription of heterologous amorpha-4,11-diene monooxygenase and cytochrome P450 reductase genes in transgenic lines was confirmed using high-resolution melting analysis. Artemisinin accumulation was detected using GC-MS in White Snowdon plants, but not in Egyptianka ones, thereby demonstrating the possibility of transplanting active artemisinin biosynthetic pathway into chrysanthemum. Ways of increasing its content in producer plants are discussed.

scholarly journals Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media

2021 ◽  
Vol 9 (2) ◽  
pp. 109-115
Author(s):  
Ahmad Saifun Naser ◽  
Muhammad Wisnu
Keyword(s):  
Tissue Culture ◽  
Callus Formation ◽  
Leaf Explants ◽  
Chrysanthemum Morifolium ◽  
Stem Explants ◽  
Appearance Time ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
Chrysanthemum Morifolium Ramat

Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.

scholarly journals Selection for wide temperature adaptation in Chrysanthemum morifolium (Ramat.) Hemsl.

1978 ◽  
Vol 26 (1) ◽  
pp. 110-118
Author(s):  
J. de Jong
Keyword(s):  
Low Temperature ◽  
Chrysanthemum Morifolium ◽  
Temperature Adaptation ◽  
Optimum Temperature ◽  
Selected Abstract ◽  
Days To Flowering ◽  
Selection For ◽  
High Or Low Temperature ◽  
Chrysanthemum Morifolium Ramat ◽  
Short Days

Rooted cuttings of commercial cvs were grown to flowering at five temperatures and the the number of short days to flowering was recorded. The optimum temperature for rapid flowering varied between cvs. The number of days to flowering at the optimum temperature was not related to the delay in flowering caused by either high or low temperature. In many cvs the delay in flowering at low temperature was accompanied by a similar delay at high temperature. It was concluded that for the character 'time to flowering' genotypes should preferably be selected at low temperatures. If low temperature cannot be realized, only rapidly flowering genotypes should be selected. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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