scholarly journals Suppression of Airway Allergic Reactions by a Photocatalytic Filter Using Mouse Model

Toxics ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 40
Author(s):  
Taisuke Tomonaga ◽  
Hiroto Izumi ◽  
Chinatsu Nishida ◽  
Kaori Kato ◽  
Kazuhiro Yatera ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Mouse Model ◽  
Respiratory Tract ◽  
Bronchoalveolar Lavage ◽  
Experimental Model ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Specific Ige ◽  
Allergic Reactions ◽  
Living Organisms

Photocatalytic filters installed in air purifiers have been used to purify spaces by decomposing allergenic substances. However, we have not found any reports that evaluate the effectiveness of photocatalytic filters in suppressing allergic reactions in living organisms. In this study, we intratracheally instilled ovalbumin (OVA) into OVA-sensitized mice after the OVA was photocatalyzed by a titanium dioxide (TiO2) filter, and verified the experimental model for evaluating the allergy-suppressing effect of photocatalysts. Mice were sensitized to OVA (10 µg/mouse) four times, and were intratracheally instilled with OVA (10 µg/mouse) after photocatalysis three times. Non-sensitized animals were instilled with normal saline following the same exposure schedule. The mice were dissected 24 h after final exposure. The OVA after photocatalysis significantly decreased the number of eosinophils in bronchoalveolar lavage fluid, and the concentration of OVA-specific IgE and IgG1 in serum, which were elevated in untreated OVA. Moreover, our experimental model showed the suppression of allergic reactions in mice, along with the decomposition of OVA after photocatalysis using the photocatalytic filter. Taken together, our experimental model for evaluating allergic reactions in the respiratory tract suggested that the allergy-suppressing effect of the photocatalytic filter can be evaluated.

Analysis of bronchoalveolar lavage fluid in a mouse model of bronchial asthma and H1N1 2009 infection

Cytokine ◽  
2013 ◽  
Vol 63 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Seigo Okada ◽  
Shunji Hasegawa ◽  
Hideki Hasegawa ◽  
Akira Ainai ◽  
Ryo Atsuta ◽  
...  
Keyword(s):  
Mouse Model ◽  
Bronchoalveolar Lavage ◽  
Bronchial Asthma ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid

A One Health Approach to Simple Liquid Sample Handling for Respiratory Based -Omics Analysis; Tracheal Wash and Bronchoalveolar Lavage Fluid

2021 ◽  
Author(s):  
Anna E. Karagianni ◽  
Samantha L. Eaton ◽  
Dominic Kurian ◽  
Eugenio Cillán-Garcia ◽  
Jonathan Twynam-Perkins ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Bronchoalveolar Lavage ◽  
Airway Inflammation ◽  
Lower Respiratory Tract ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Lower Airway ◽  
Simple Liquid ◽  
Sample Handling ◽  
Sequential Screening

Abstract Airway inflammation is highly prevalent in horses, with the majority of non-infectious cases being defined as equine asthma. Currently, cytological analysis of airway derived samples is the principal method of assessing lower airway inflammation. Samples can be obtained by tracheal wash (TW) or by lavage of the lower respiratory tract (bronchoalveolar lavage fluid; BALF). Although BALF cytology carries significant diagnostic advantages over TW cytology, sample acquisition is invasive, making it prohibitive for routine and sequential-screening of airway health. The aim of this study was to establish a robust protocol to isolate macrophages, protein and RNA for molecular characterisation of TW samples and demonstrate the applicability of sample handling to rodent and human pediatric bronchoalveolar lavage fluid isolates. TW samples provided a good quality and yield of both RNA and protein for downstream transcriptomic/proteomic analyses. The sample handling methodologies were successfully applicable to BALF for rodent and human research. TW samples represent a rich source of airway cells, and molecular analysis to facilitate and study airway inflammation, based on both transcriptomic and proteomic analysis. This study provides a necessary methodological platform for future transcriptomic and/or proteomic studies on equine lower respiratory tract secretions and BALF samples from humans and mice.

IgE-bearing Cells in Bronchoalveolar Lavage Fluid and Allergen-specific IgE Levels in Sera from RAO-affected Horses

2007 ◽  
Vol 54 (1) ◽  
pp. 40-47 ◽  
Author(s):  
F. Künzle ◽  
V. Gerber ◽  
A. Van Der Haegen ◽  
B. Wampfler ◽  
R. Straub ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Specific Ige

scholarly journals Azithromycin Attenuates Lung Inflammation in a Mouse Model of Ventilator-Associated Pneumonia by Multidrug-Resistant Acinetobacter baumannii

2013 ◽  
Vol 57 (8) ◽  
pp. 3883-3888 ◽  
Author(s):  
Koichi Yamada ◽  
Katsunori Yanagihara ◽  
Norihito Kaku ◽  
Yosuke Harada ◽  
Yohei Migiyama ◽  
...  
Keyword(s):  
Mouse Model ◽  
Bronchoalveolar Lavage ◽  
Acinetobacter Baumannii ◽  
Lung Inflammation ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Multidrug Resistant ◽  
Control Group ◽  
Ventilator Associated Pneumonia ◽  
Content Type

ABSTRACTAcinetobacter baumanniiis one of the main pathogens that cause ventilator-associated pneumonia (VAP) and is associated with a high rate of mortality. Little is known about the efficacy of macrolides againstA. baumannii. In order to confirm the efficacy of azithromycin (AZM) against VAP caused by multidrug-resistantA. baumannii(MDRAB), we used a mouse model that mimics VAP by placement of a plastic tube in the bronchus. AZM (10 and 100 mg/kg of body weight) was administered subcutaneously every 24 h beginning at 3 h after inoculation. Phosphate-buffered saline was administered as the control. Survival was evaluated over 7 days. At 48 h postinfection, mice were sacrificed and the numbers of viable bacteria in lungs and bronchoalveolar lavage fluid were compared. Histopathological analysis of lung specimens was also performed. The treatment groups displayed significantly longer survival than the control group (P< 0.05). AZM did not have an antimicrobial effect. Histopathological examination of lung specimens indicated that the progression of lung inflammation was prevented in the AZM-treated groups. Furthermore, total cell and neutrophil counts, as well as cytokine levels, in bronchoalveolar lavage fluid were significantly decreased (P< 0.05) in the AZM-treated groups. AZM may have a role for the treatment of VAP with MDRAB because of its anti-inflammatory effects.

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