scholarly journals Mechanisms of Increased Indoxacarb Toxicity in Methoxyfenozide-Resistant Cotton Bollworm Helicoverpa armigera (Lepidoptera: Noctuidae)

Toxics ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 71
Author(s):  
Qinqin Wang ◽  
Changhui Rui ◽  
Qiyuan Wang ◽  
Li Wang ◽  
Fugen Li ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Cross Resistance ◽  
Glutathione S Transferase ◽  
Cytochrome P450 Activity ◽  
Helicoverpa Armígera ◽  
Care Activity ◽  
Moderate Resistance ◽  
Lepidoptera Noctuidae

Indoxacarb is an important insecticide for the selective control of Helicoverpa armigera. It can be bioactivated to the more effective N-decarbomethoxylated indoxacarb (DCJW) by esterases in pests. It was observed that both field and laboratory selected populations of H. armigera showed negative cross-resistance between indoxacarb and methoxyfenozide. The Handan population exhibited moderate resistance to indoxacarb, but was susceptible to methoxyfenozide; the Baoding and Yishui populations exhibited moderate resistance to methoxyfenozide, but they were susceptible to indoxacarb. Moreover, the toxicity of indoxacarb was enhanced 1.83-fold in the laboratory methoxyfenozide-resistant H. armigera, and susceptibility to methoxyfenozide was increased 2.81-fold in the laboratory indoxacarb-resistant H. armigera. In vivo, DCJW concentrations in the susceptible and methoxyfenozide-selected (laboratory methoxyfenozide-resistant) populations were 4.59- and 4.31-fold greater than in the indoxacarb-resistant Handan population 1 h after dosing. After 2 h, the highest concentrations of DCJW and indoxacarb appeared in the methoxyfenozide-selected population. Meanwhile, increased carboxyl esterase (CarE) and decreased glutathione S-transferase (GST) activities were observed in the methoxyfenozide-selected population. However, the indoxacarb-selected (laboratory indoxacarb-resistant) and Handan populations showed a higher disappearance of indoxacarb and DCJW, and the activity of cytochrome P450 mono-oxygenase in these populations were significantly increased. This study showed that the improved toxicity of indoxacarb, as observed in the methoxyfenozide-selected H. armigera, was correlated with increased CarE activity, decreased GST activity, and the in vivo accumulation of indoxacarb and DCJW. The significantly increased cytochrome P450 activity and higher disappearance of indoxacarb and DCJW in indoxacarb-resistant H. armigera resulted in the decreased toxicity of indoxacarb.

scholarly journals Cross-Resistance Studies of Cry1Ac-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae) to Cry2Ab

2007 ◽  
Vol 100 (3) ◽  
pp. 909-915 ◽  
Author(s):  
Shudong Luo ◽  
Kongming Wu ◽  
Yan Tian ◽  
Gemei Liang ◽  
Xue Feng ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Cross Resistance ◽  
Helicoverpa Armígera ◽  
Resistant Strains ◽  
Lepidoptera Noctuidae

Effects of dietary quercetin on performance and cytochrome P450 expression of the cotton bollworm, Helicoverpa armigera

2015 ◽  
Vol 105 (6) ◽  
pp. 771-777 ◽  
Author(s):  
D. Liu ◽  
Y. Yuan ◽  
M. Li ◽  
X. Qiu
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Adult Emergence ◽  
Cotton Bollworm ◽  
Molecular Response ◽  
Terrestrial Plants ◽  
P450 Expression ◽  
Helicoverpa Armígera

AbstractQuercetin is ubiquitous in terrestrial plants. The cotton bollworm Helicoverpa armigera as a highly polyphagous insect has caused severe crop losses. Until now, interactions between this pest and quercetin are poorly understood at the biochemical and molecular levels. In this study, we investigated the in vivo effects of quercetin on performance of cotton bollworm and on cytochrome P450 (P450) expression. Deleterious effects of quercetin on the performance of the cotton bollworm, including growth, survival, pupation and adult emergence were observed after oral administration of 3 and 10 mg g−1 quercetin to larvae since the third instar, whereas no significant toxic effect was found at 0.1 mg g−1 quercetin treatment. Piperonyl butoxide treatment enhanced the toxicity of quercetin. In vitro metabolism studies showed that quercetin was rapidly transformed by gut enzymes of fifth instar larvae of the cotton bollworm. qRT–PCR results revealed that the effect of quercetin on P450 expression was tissue- and dose-specific. Quercetin regulated P450 expression in a mild manner, and it could serve as P450 inducer (CYP337B1, CYP6B6) or repressor (CYP337B1, CYP6B7, CYP6B27, CYP9A14, CYP6AE11, and CYP4M7). These findings are important for advancing our understanding of the biochemical and molecular response of insects to plant toxins and have implications for a smart pest control.

scholarly journals Efficacy of a Combined Treatment of Neem Oil Formulation and Endosulfan against Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae)

2014 ◽  
Vol 6 ◽  
pp. IJIS.S13608 ◽  
Author(s):  
Abdul Rashid War ◽  
Michael Gabriel Paulraj ◽  
Barkat Hussain ◽  
Tariq Ahmad ◽  
Mohd Yousf War ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Digestive Enzymes ◽  
Combined Treatment ◽  
Neem Oil ◽  
Antifeedant Activity ◽  
Glutathione S Transferase ◽  
Helicoverpa Armígera ◽  
Oil Formulation ◽  
Lepidoptera Noctuidae ◽  
Gst Activity

Efficacy of the combined treatment of a neem oil formulation and endosulfan on feeding and midgut enzyme activities of Helicoverpa armigera larvae was studied. The antifeedant activity was recorded at 24 h after treatment and the activities of midgut digestive (total serine protease and trypsin) and detoxifying (esterase and glutathione-S-transferase) enzymes were estimated at 72 h after treatment. The antifeedant activity in endosulfan + neem oil formulation (endosulfan 0.01% and neem oil formulation 1% at 1:1 ratio) was 85.34%, significantly greater than in individual treatments. Midgut digestive enzymes and EST activities were significantly reduced and the GST activity significantly increased in the combined treatment of endosulfan + neem oil formulation, thus showing increased effect of the combined treatment of the two pesticides. These results suggest that neem oil can be used in combination with endosulfan to reduce its quantity.

Role of induced glutathione-S-transferase from Helicoverpa armigera (Lepidoptera: Noctuidae) HaGST-8 in detoxification of pesticides

2018 ◽  
Vol 147 ◽  
pp. 612-621 ◽  
Author(s):  
Chaitali P. Labade ◽  
Abhilash R. Jadhav ◽  
Mehul Ahire ◽  
Smita S. Zinjarde ◽  
Vaijayanti A. Tamhane
Keyword(s):  
Helicoverpa Armigera ◽  
Glutathione S Transferase ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae

Pyrethroid resistance of Helicoverpa armigera (Lepidoptera: Noctuidae) in Pakistan

1997 ◽  
Vol 87 (4) ◽  
pp. 343-347 ◽  
Author(s):  
M. Ahmad ◽  
M. I. Arif ◽  
M. R. Attique
Keyword(s):  
Helicoverpa Armigera ◽  
General Trend ◽  
Pyrethroid Resistance ◽  
Physiological Mechanisms ◽  
Resistance Factors ◽  
Lambda Cyhalothrin ◽  
The Status ◽  
Helicoverpa Armígera ◽  
Moderate Resistance ◽  
Lepidoptera Noctuidae

AbstractThe status of pyrethroid resistance in some field populations of Helicoverpa armigera (Hübner) from Pakistan was determined using an IRAC leaf-dip method. Resistance factors varied between populations, and the general trend was for a moderate to high resistance to cypermethrin and cyfluthrin; a low to moderate resistance to deltamethrin and alpha-cypermethrin; and a comparatively low resistance to bifenthrin, lambda-cyhalothrin and zeta-cypermethrin. Depending on which physiological mechanisms are shown to be present in Pakistani field strains, the latter group of pyrethroids may serve as useful tools in the management of insecticide resistance.

scholarly journals Insecticide resistance in field populations of the tomato fruitworm, Helicoverpa armigera, from Senegal

2020 ◽  
Vol 14 (1) ◽  
pp. 181-191
Author(s):  
Serigne Omar Sene ◽  
Etienne Tendeng ◽  
Mamadou Diatte ◽  
Serigne Sylla ◽  
Babacar Labou ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Helicoverpa Armigera ◽  
Cropping Systems ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Metabolic Resistance ◽  
Resistance Evolution ◽  
The North ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae

Monitoring of the evolution of insecticide resistance in the field is crucial to prevent pest control issues. The present study was conducted to assess insecticide resistance status of the fruitworm, Helicoverpa armigera (Hübner) (Lepidoptera, Noctuidae), the most destructive pest of field-grown tomato in Senegal. A sample of 11- 15 field populations were monitored for their susceptibility to abamectin, deltamethrin, and profenofos, using a standard leaf-dip bioassay method. Resistance ratios ranged from 1- to 30-fold to abamectin (4/15 populations with RR>10), 7- to 112-fold to deltamethrin (11/12 populations with RR>10), and 1- to 29-fold to profenofos (3/11 populations with RR>10). This indicates that resistance evolution to deltamethrin was widespread among field populations of H. armigera. However, an increasing trend of resistance to deltamethrin was observed from the South to the North of Niayes. Susceptibility to abamectin and profenofos was generally high but showed that resistance might be evolving within some populations. In addition, signs of cross-resistance to abamectin were detected, suggesting possible metabolic resistance mechanisms already selected in pyrethroid-resistant populations. The recorded high levels of pyrethroids resistance are a concern for the control of H. armigera in Senegal as the country is being currently embarking into economic expansion of tomato cropping systems. © 2020 International Formulae Group. All rights reserved. Keywords: Insecticide resistance, pyrethroids, avermectins, OPs, Helicoverpa armigera, West Africa

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