scholarly journals Multiple Human Papilloma Virus (HPV) Infections Are Associated with HSIL and Persistent HPV Infection Status in Korean Patients

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1342
Author(s):  
Moonsik Kim ◽  
Nora Jee-Young Park ◽  
Ji Yun Jeong ◽  
Ji Young Park
Keyword(s):  
Human Papilloma Virus ◽  
Hpv Infection ◽  
Vaginal Swab ◽  
Chain Reaction ◽  
Clinicopathologic Characteristics ◽  
Papilloma Virus ◽  
Hpv Types ◽  
Polymerase Chain ◽  
High Risk Hpv

Infections with multiple human papilloma virus (HPV) types have been reported, but their role in cervical carcinogenesis has not been fully elucidated. In this study, 236 cases with multiple HPV infection were examined and compared to 180 cases with single HPV infection. HPV genotyping was performed with cervico-vaginal swab specimens using multiplex (real-time) polymerase chain reaction (PCR). In multiple HPV infection, the most prevalent HPV genotype was HPV 53, followed by HPV 16, 58, 52, and 68. HPV 33, 35, 39, 51, 52, 53, 58, and 68 were high-risk-HPV (HR-HPV) genotypes that were more frequently detected in multiple HPV infection compared to that in single HPV infection. The association between multiple HPV infection and high-grade SIL (HSIL) was significantly stronger compared to that of single HPV infection and HSIL (p = 0.002). Patients with multiple HPV infection displayed persistent and longer duration of the HPV infection compared to patients with single HPV infection. Multiple HPV infections have distinct clinicopathologic characteristics. Since it is associated with persistent HPV infection, HSIL, and different HR-HPV strains in contrast to single HPV infection, the presence of multiple HPV infection should be reported; close follow up is warranted.

Human Papillomavirus Infection of the Epididymis and Ductus Deferens: An Evaluation by Nested Polymerase Chain Reaction

2003 ◽  
Vol 127 (11) ◽  
pp. 1471-1474 ◽  
Author(s):  
Alexandr Švec ◽  
Iva Mikyšková ◽  
Ondřej Hes ◽  
Ruth Tachezy
Keyword(s):  
Polymerase Chain Reaction ◽  
High Risk ◽  
Hpv Infection ◽  
Low Risk ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Ductus Deferens ◽  
Hpv Types ◽  
Polymerase Chain ◽  
High Risk Hpv

Abstract Context.—Human papillomaviruses (HPVs) play an important role in the etiology of squamous cell carcinoma of the uterine cervix. The possible role of the male urogenital tract as a reservoir of HPV infection is not fully understood. We inferred from our previous observation of HPV-31 in epididymal tissue in a case of chronic epididymitis that HPV might be commonly present in cases of epididymitis caused by sexually transmitted pathogens. Objective.—To assess the presence of HPV in the epididymis and ductus deferens in nontuberculous epididymitis. Design.—Epididymal samples obtained from 17 patients and epididymal and ductus deferens samples from 5 patients surgically treated for nontuberculous epididymitis were analyzed by nested polymerase chain reaction for the presence of HPV DNA. In positive samples, the HPV type was determined by DNA sequencing. Setting.—Tertiary-care academic hospital and national reference laboratory for papillomaviruses. Results.—Low-risk HPV type 6 and high-risk HPV types 16, 33, 35, 55, and 73 were detected in 7 patients (31%). Neither koilocytes nor dysplastic changes were found in the epididymis and ductus deferens. Conclusion.—Low-risk and high-risk HPV types were detected in the epididymis and ductus deferens of patients with nontuberculous epididymitis. The infection was not accompanied by koilocytic atypia or dysplasia. Our findings support the hypothesis that the male urogenital tract serves as a reservoir of HPV infection.

Human papilloma virus detection in liquid cytology, in situ hybridization and polymerase chain reaction

2005 ◽  
Vol 446 (2) ◽  
pp. 202-203 ◽  
Author(s):  
F. Alameda ◽  
L. Pijuan ◽  
L. Ferrer ◽  
M. L. Mari�oso ◽  
M. Muset ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Human Papilloma Virus ◽  
Virus Detection ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain

scholarly journals Prevalence and Genotyping of Human Papillomavirus (HPV) in Female with High-Risk Behaviour in Dhaka, Bangladesh

1970 ◽  
Vol 25 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Tahmina Sultana ◽  
Mohsina Huq ◽  
Anadil Alam ◽  
Dipak Kumar Mitra ◽  
Donald James Gomes
Keyword(s):  
Cervical Cancer ◽  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
High Risk ◽  
General Population ◽  
Sex Workers ◽  
Chain Reaction ◽  
Hpv Types ◽  
Polymerase Chain ◽  
High Risk Hpv

In developing countries, cervical cancer is the most common cause of cancer related to mortality in women. But the epidemiology of human papillomavirus (HPV) in different areas of Bangladesh is largely unknown both in risk groups and in the general population. The objective of the present study was to determine the risk factors associated with having HPV and the prevalence of high-risk HPV types among women with highrisk behaviour and to assess its potential impact on preventive strategies as the sex workers are at increased risk for sexually transmitted infections (STI), HPV and hence cervical cancer. Cervical swab from 293 sex workers in Dhaka City between August and September 2003 and between February 2005 and May 2006 were screened for HPV DNA using an HPV short fragment (E6) polymerase chain reaction (PCR) based assay. HPV positive samples were genotyped with nested multiplex polymerase chain reaction (NMPCR) for the highrisk types. The overall HPV prevalence in sex workers was 75.8%, whereas for the high risk type it was 49.8%. Prevalence of single genotype and multiple types of HPV was 33.1 and 16.7% respectively. The most prevalent high-risk HPV types, in order of prevalence rate, were HPV16, HPV18, HPV58, HPV45, HPV31 and HPV33. Both HPV 16 and HPV 18 were present in 21% of the cases. Targeting HPV 16 and 18 with prophylactic vaccines could possibly have an important impact on the incidence of invasive cervical carcinoma in this group of women. Primary prevention and cervical cancer screening programmes should be optimized more and run yearly among the general population. It is proposed to screen sex workers when they enter prostitution regardless of their age. Keywords: Human papillomavirus (HPV); High-risk HPV types; Cervical cancer; Sex workersDOI: http://dx.doi.org/10.3329/bjm.v25i1.4861 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 65-68

Asymptomatic Penile HPV Infection: A Prospective Study

2000 ◽  
Vol 11 (2) ◽  
pp. 80-84 ◽  
Author(s):  
A Wikström ◽  
C Popescu ◽  
O Forslund
Keyword(s):  
Hpv Infection ◽  
Sexually Active ◽  
Hpv Dna ◽  
Persistent Infections ◽  
Repeated Sampling ◽  
Pcr Method ◽  
Hpv Types ◽  
Polymerase Chain ◽  
A Prospective Study

The occurrence of human papillomavirus (HPV) among males was analysed with the polymerase chain reaction (PCR) method. Penile brush samples were taken once from 147 males attending for a control or for HPV non-related reasons, and consecutive samples were collected from 88 males re-attending the clinic. Of the males attending once, 13% (19/147) were HPV DNA positive and among the re-attenders 14% (12/88) were initially positive as compared with 33% (29/88) who were positive at least at one visit. Totally, 22 different HPV types were detected of which HPV 16 was most common, found in 6.4% (15/235), followed by HPV 42 found in 3.8% (9/235). Among 14 HPV-positive males with at least one follow-up, 7 had persistent infections with at least one HPV type, and transient HPV types were observed in 9; but in 5 of them new types appeared at follow-up. Among sexually-active males subclinical/latent HPV infection is common and repeated sampling increases its prevalence.

scholarly journals Associations among Serum Lipocalin-2 Concentration, Human Papilloma Virus, and Clinical Stage of Cervical Cancer

Medicina ◽  
2019 ◽  
Vol 55 (6) ◽  
pp. 229 ◽  
Author(s):  
Agnė Vitkauskaitė ◽  
Joana Celiešiūtė ◽  
Saulius Paškauskas ◽  
Erika Skrodenienė ◽  
Rūta Jolanta Nadišauskienė ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papilloma Virus ◽  
Clinical Stage ◽  
Stage Iv ◽  
Hpv Infection ◽  
Control Group ◽  
Lipocalin 2 ◽  
Papilloma Virus ◽  
Tissue Biopsy ◽  
Hpv Types

Background and objective: Lipocalin 2 (LCN2) has an oncogenic role in promoting tumorigenesis through enhancing tumor cell proliferation and the metastatic potential. The aim of our study was to determine whether serum LCN2 could serve as a diagnostic marker of cervical cancer (CC) and to evaluate the correlation between its serum concentration, the clinical stage of the cancer and Human Papilloma Virus HPV infections in women. Materials and methods: A total of 33 women with histologically proven cervical cancer (CC), 9 women with high- grade cervical intraepithelial neoplasia (HSIL) and 48 healthy women (NILM) were involved in the study. A concentration of LCN2 was assayed with the Magnetic LuminexR Assay multiplex kit. An HPV genotyping kit was used for the detection and differentiation of 15 high-risk (HR) HPV types in the liquid-based cytology medium (LBCM) and the tissue biopsy. Results: The majority (84.8%) of the women were infected by HPV16 in the CC group, and there was no woman with HPV16 in the control group (P < 0.01). Several types of HR HPV were found more often in the LBCM compared to in the tissue biopsy (P = 0.044). HPV16 was more frequently detected in the tissue biopsy than the LBCM (P < 0.05). The LCN2 level was higher in HPV-positive than in HPV-negative women (P = 0.029). The LCN2 concentration was significantly higher in women with stage IV than those with stage I CC (P = 0.021). Conclusions: Many HR HPV types, together with HPV16/18, can colonize the vagina and cervix, but often HPV16 alone penetrates into the tissue and causes CC. The serum LCN2 concentration was found to be associated not only with HR HPV infection, irrespective of the degree of cervical intraepithelial changes, but also with advanced clinical CC stage. LCN2 could be used to identify patients with advanced disease, who require a more aggressive treatment.

Uptake of exogenous human papilloma virus L1 DNA by oocytes and detection by the polymerase chain reaction

1992 ◽  
Vol 9 (6) ◽  
pp. 531-533 ◽  
Author(s):  
Philip J. Chan ◽  
Brian C. Su ◽  
Donald R. Tredway ◽  
Majid Seraj ◽  
Ibrahim M. Seraj ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain

Human papilloma viruses: a study of their prevalence in the normal larynx

1994 ◽  
Vol 108 (4) ◽  
pp. 319-320 ◽  
Author(s):  
Desmond A. Nunez ◽  
Siân M. Astley ◽  
Fraser A. Lewis ◽  
Michael Wells
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Tissue ◽  
Hpv Infection ◽  
Proteinase K ◽  
Chain Reaction ◽  
Human Papilloma Viruses ◽  
Hpv Types ◽  
Polymerase Chain

AbstractThe association of human papilloma viruses (HPV) with laryngopharyngeal squamous cell carcinoma is under investigation. The suitability of control tissue in the reported series, invariably obtained from histologically normal tissue adjacent to a squamous cell carcinoma or from patients with benign laryngopharyngeal disease, is questionable. The present study determined the prevalence of HPV in a series of normal larynges.Twelve autopsy larynges were collected. DNA was obtained by SDS proteinase K digestion. Evidence of HPV infection was documented by the polymerase chain reaction using oligonulceotide primers complementary to sequences in the E6 region of HPV types 11, 16 and 18.Four female and eight male larynges, mean age 65 years (sd=16 years) were collected 72 hours postmortem (median value). HPV type 11 was isolated from three specimens. A 25 per cent prevalence rate for HPV 11 was found. No other HPV types were isolated.

scholarly journals Presencia de lesiones preinvasoras e invasoras de cérvix, relación con el virus papiloma humano y factores epidemiológicos en Mérida, Venezuela

2018 ◽  
Vol 2 (2) ◽  
pp. 92
Author(s):  
Luis Eduardo Téllez Gil ◽  
Elvia María Michelli Viña ◽  
Diana Estela Callejas Monsalve ◽  
Mike Contreras Colmenares ◽  
María Eugenia Cavazza Porro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Viral Infection ◽  
Smoking Habit ◽  
Multiple Infections ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Collection Device ◽  
Dna Collection ◽  
Polymerase Chain

  Las lesiones de cérvix se han asociado a infección por Virus Papiloma Humano (VPH). 300 mujeres mayores de quince años que acudieron al Hospital Universitario de Los Andes (HULA), fueron estudiadas para identificar lesiones, detectar y tipificar VPH, y determinar factores asociados. Se realizó citología, colposcopia, cepillados cervicales utilizando (DNA collection device Digene®) y biopsias en los casos pertinentes. Se aisló el ADN mediante (QIAamp DNA Mini Kit QIAGEN®), siendo cuantificado y almacenado a -20 ºC. Se detectó VPH por Reacción en Cadena de la Polimerasa (PCR) de regiones L1 y E6/E7. La genotipificación por PCR anidada múltiple E6/E7, C. trachomatis se detectó por PCR. El VPH se detectó en 35 % (105) muestras, 88,46 % (92/105) fueron positivas para al menos uno de los genotipos evaluados. VPHAR se encontraron en 97,82 %, (90/92), VPH18 en 82 % (74/90), VPH16 en 44 % (40/90). 56,52 % (52/92) correspondieron a infecciones múltiples, VPH18/16 (20/52) fue la más frecuente. C. trachomatis se detectó en 9 % (27/300) pacientes. La citología mostró cambios sugestivos de infección en solo 16,35 % de las pacientes VPH positivas. 17/18 biopsias sugirieron infección viral y fueron positivas para VPH AR por biología molecular (94,44 %). La colposcopia sugirió infección viral en 46,15 %. El 66,34 % de pacientes fueron menores de 35 años. Se encontró relación estadísticamente no significativa entre infección por VPH, número de parejas sexuales, coinfección con C. trachomatis y hábito tabáquico. Estos resultados muestran elevada frecuencia de infección por VPH AR, asociada a factores epidemiológicos, cuyo diagnóstico certero y tratamiento oportuno son claves en la prevención de su transmisión y del desarrollo de lesiones en cérvix.   Palabras clave: Cáncer cervical, virus papiloma humano, reacción en cadena de la polimerasa.   Abstract   Cervical lesions have been associated with infection by Human Papilloma Virus (HPV). Three hundred women older than 15 years old who attended at the Hospital Universidad de Los Andes (HULA), were studied to identify lesions, detect and typify HPV, and determine associated factors. Cytology, colposcopy, cervical brushing using (DNA collection device Digene®) and biopsies were performed in the pertinent cases. DNA was isolated by (QIAamp DNA Mini Kit QIAGEN®), being quantified and stored at -20 ° C. HPV was detected by Polymerase Chain Reaction (PCR) of regions L1 and E6 / E7. The genotyping by multiple nested PCR E6 / E7, C. trachomatis was detected by PCR. HPV was detected in 35% (105) samples, 88.46% (92/105) were positive for at least one of the genotypes evaluated. VPHAR were found in 97.82% (90/92), HPV18 in 82% (74/90), HPV16 in 44% (40/90). 56.52% (52/92) corresponded to multiple infections, HPV18 / 16 (20/52) was the most frequent. C. trachomatis was detected in 9% (27/300) patients. The cytology showed changes suggestive of infection in only 16.35% of the HPV positive patients. 17/18 biopsies suggested viral infection and were positive for ARV HPV by molecular biology (94.44%). Colposcopy suggested viral infection in 46.15%. 66.34% of patients were under 35 years old. A statistically non-significant relationship was found between HPV infection, number of sexual partners, coinfection with C. trachomatis and smoking habit. These results show high frequency of infection by HPV AR, associated with epidemiological factors, whose accurate diagnosis and timely treatment are key in the prevention of its transmission and the development of lesions in the cervix.   Keywords: Cervical cancer, human papilloma virus, polymerase chain reaction.

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