Methods for improving the functional and metabolic status of spermatozoa with a low-intensity laser in vitro

2020 ◽  
pp. 24-31
Author(s):  
A. A. Khisamova
Keyword(s):  
Seminal Fluid ◽  
Physiological Activity ◽  
Radiant Energy ◽  
Pulse Generation ◽  
Metabolic Status ◽  
Healthy Human ◽  
Low Intensity ◽  
Sperm Suspension ◽  
Intensity Laser

A method for improving the functional and metabolic status of spermatozoa, including increased motion and physiological activity of spermatozoa obtained from the seminal fluid of a healthy human in vitro and including the red wavelength radiation of a low-intensity laser, is characterized in that spermatozoa isolated from a healthy donor are washed with saline twice by centrifugation for 10 minutes at 1500 rpm and the sperm suspension is impacted on with a 632 nm semiconductor laser in the mode of variable pulse generation, with the pulse width of 2 ns, the frequency of 100 Hz, the radiant energy density of 0.56 j/cm2 , the exposure time of 1 minute, expressed in increased motion and physiological activity of spermatozoa.

scholarly journals In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel

2010 ◽  
Vol 24 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Caroline Maria Gomes Dantas ◽  
Carolina Lapaz Vivan ◽  
Leila Soares Ferreira ◽  
Patricia Moreira de Freitas ◽  
Márcia Martins Marques
Keyword(s):  
Low Intensity ◽  
Vitro Effect ◽  
Intensity Laser

scholarly journals Análise da terapia a laser de baixa intensidade em culturas in vitro de bactérias e fungo

2015 ◽  
Vol 13 ◽  
pp. 304
Author(s):  
Rodrigo Antonio Carvalho Andraus ◽  
Luciana Prado Maia ◽  
João Paulo Manfré Dos Santos ◽  
Amanda Rezende Mesquita ◽  
Thaynara Gonzaga Santos ◽  
...  
Keyword(s):  
Laser Therapy ◽  
Irradiation Time ◽  
Bacterial Species ◽  
Klebsiella Pneumonia ◽  
Stimulant Effect ◽  
Low Intensity ◽  
Low Intensity Laser Therapy ◽  
Intensity Laser ◽  
Group 1

Introduction: Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Objective: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 Enterococcus plates) and gram negative bacterias (3 Escherichia coli, 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans. Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A1, A2/B, B1, B2/C, C1, C2), and points A/B/C reciebed only one application, points A1,B1,C1 received 2 applications and points A2,B2,C2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm² dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. Results: In all the groups, no zone of inhibition nor growth were observed, indicating no bactericidal/fungicidal and/or bactericidal effect, in other words, in all groups and locations with different time exposures (A - 30 mW, B - and C 60 mW - 100 mW) the growth of bacteria and fungi were equal. Conclusion: Low intensity laser therapy (808nm and 660nm) did not produce bactericidal and/or bacteriostatic effects, and even no bio-stimulant effect on the bacteria.

scholarly journals Low intensity laser irradiation in myogenic precursor cells: an in vitro study (732.4)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
André Amaral ◽  
Heloisa Araújo ◽  
Nivaldo Parizotto ◽  
Tânia Salvini
Keyword(s):  
Laser Irradiation ◽  
In Vitro Study ◽  
Precursor Cells ◽  
Vitro Study ◽  
Low Intensity ◽  
Intensity Laser ◽  
Myogenic Precursor

MORPHOFUNCTIONAL PARAMETERS OF CATTLE ERYTHROCYTES UNDER STRESS AND ITS CORRECTION BY LOW-INTENSITY LASER RADIATION

1970 ◽  
pp. 67-71
Author(s):  
М. N. Ivashchenko ◽  
А. V. Deryugina ◽  
P. S. Ignatiev ◽  
V. B. Metelin ◽  
М. N. Talamanova ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Erythrocyte Membrane ◽  
Control Level ◽  
Experimental Studies ◽  
Erythrocyte Membranes ◽  
Physical Factors ◽  
Low Intensity ◽  
Intensity Laser ◽  
Technological Stress

The study investigated the content of malondialdehyde (MDA), the electrophoretic mobility of erythrocytes (EPME), the spectrum of erythrocyte membrane proteins and the morphology of cattle erythrocytes in in vitro experiments under stress and exposure to low-intensity laser radiation (LLLT). Clinical and experimental studies carried out in the last decade indicate the possibility of modulating the organism adaptive reactions when exposed to such physical factors as low-intensity laser radiation. The work showed that the effect of LLLT on the blood of stressed animals caused the restoration of the studied parameters to the level of physiological norms, while in animals that underwent technological stress, EPME was reduced by 31%, MDA concentration was increased by 65%. The effect of LLLT on the blood of unstressed animals did not lead to a change in EPME and MDA concentration. The study of the protein spectrum of erythrocyte membrane of animals subjected to technological stress revealed that the content of spectrin decreased by 16%, glycophorin C increased by 35%, the morphology of erythrocytes after stress was characterized by an increase in the number of echinocytes, stomatocytes and degeneratively altered erythrocytes. Under the LLLT action on the cow erythrocytes after stress there was a restoration of the morphology of cells and the content of proteins of erythrocyte membranes to the control level.

scholarly journals Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

2014 ◽  
Vol 41 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Pâmela Rosa Pereira ◽  
Josue Bruginski De Paula ◽  
Juliette Cielinski ◽  
Marcelo Pilonetto ◽  
Luiz Carlos Von Bahten
Keyword(s):  
Laser Therapy ◽  
Control Group ◽  
Type I ◽  
Low Intensity ◽  
Infected Wounds ◽  
Male Wistar Rats ◽  
Low Intensity Laser Therapy ◽  
Intensity Laser

OBJECTIVE: to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections. METHODS: in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm2 daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated. RESULTS: in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034). CONCLUSION: low-intensity Laser Therapy applied with a wavelength of 904nm and dose 3J/cm2 did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment.

Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro

2005 ◽  
Vol 36 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Pete Moore ◽  
Tisha D. Ridgway ◽  
Russell G. Higbee ◽  
Eric W. Howard ◽  
Michael D. Lucroy
Keyword(s):  
Cell Proliferation ◽  
Laser Irradiation ◽  
Low Intensity ◽  
Proliferation In Vitro ◽  
Intensity Laser

In vitro effect of phototherapy with low-intensity laser on HSV-1 and epithelial cells

2007 ◽  
Author(s):  
Fernanda P. Eduardo ◽  
Dolores U. Mehnert ◽  
Telma A. Monezi ◽  
Denise M. Zezell ◽  
Mark M. Schubert ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Low Intensity ◽  
Vitro Effect ◽  
Intensity Laser ◽  
Hsv 1
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