In vitro effect of phototherapy with low-intensity laser on HSV-1 and epithelial cells

2007 ◽  
Author(s):  
Fernanda P. Eduardo ◽  
Dolores U. Mehnert ◽  
Telma A. Monezi ◽  
Denise M. Zezell ◽  
Mark M. Schubert ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Low Intensity ◽  
Vitro Effect ◽  
Intensity Laser ◽  
Hsv 1

scholarly journals In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel

2010 ◽  
Vol 24 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Caroline Maria Gomes Dantas ◽  
Carolina Lapaz Vivan ◽  
Leila Soares Ferreira ◽  
Patricia Moreira de Freitas ◽  
Márcia Martins Marques
Keyword(s):  
Low Intensity ◽  
Vitro Effect ◽  
Intensity Laser

scholarly journals The In Vitro Effect of Steroid Hormones, Arachidonic Acid, and Kinases Inhibitors on Aquaporin 1, 2, 5, and 7 Gene Expression in the Porcine Uterine Luminal Epithelial Cells during the Estrous Cycle

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 832
Author(s):  
Damian Tanski ◽  
Agnieszka Skowronska ◽  
Malgorzata Tanska ◽  
Ewa Lepiarczyk ◽  
Mariusz T. Skowronski
Keyword(s):  
Arachidonic Acid ◽  
Epithelial Cells ◽  
Steroid Hormones ◽  
Estrous Cycle ◽  
Kinase Inhibitor ◽  
Mapk Signaling ◽  
Mapk Kinase ◽  
Vitro Effect ◽  
Luminal Epithelial Cells

Aquaporins (AQPs) are integral membrane proteins, which play an important role in water homeostasis in the uterus. According to the literature, the expression of aquaporins in reproductive structures depends on the local hormonal milieu. The current study investigated the effect of selected PKA kinase inhibitor H89 and MAPK kinase inhibitor PD98059, on the expression of AQP1, 2, 5, and 7, and steroid hormones (E2), progesterone (P4), and arachidonic acid (AA) in the porcine endometrium on days 18–20 and 2–4 of the estrous cycle (the follicular phase where estrogen and follicle-stimulating hormone (FSH) are secreted increasingly in preparation for estrus and the luteal phase where the ovarian follicles begin the process of luteinization with the formation of the corpus luteum and progesterone secretion, respectively). The luminal epithelial cells were incubated in vitro in the presence of the aforementioned factors. The expression of mRNA was determined by the quantitative real-time PCR technique. In general, in Experiment 1, steroid hormones significantly increased expression of AQP1, 2, and 5 while arachidonic acid increased expression of AQP2 and AQP7. On the other hand, MAPK kinase inhibitor significantly decreased the expression of AQP1 and 5. In Experiment 2, E2, P4, or AA combined with kinase inhibitors differentially affected on AQPs expression. E2 in combination with PKA inhibitor significantly decreased expression of AQP1 but E2 or P4 combined with this inhibitor increased the expression of AQP5 and 7. On the contrary, E2 with PD98059 significantly increased AQP5 and AQP7 expression. Progesterone in combination with MAPK kinase inhibitor significantly downregulated the expression of AQP5 and upregulated AQP7. Arachidonic acid mixed with H89 or PD98059 caused a decrease in the expression of AQP5 and an increase of AQP7. The obtained results indicate that estradiol, progesterone, and arachidonic acid through PKA and MAPK signaling pathways regulate the expression of AQP1 and AQP5 in the porcine luminal epithelial cells in the periovulatory period.

scholarly journals Análise da terapia a laser de baixa intensidade em culturas in vitro de bactérias e fungo

2015 ◽  
Vol 13 ◽  
pp. 304
Author(s):  
Rodrigo Antonio Carvalho Andraus ◽  
Luciana Prado Maia ◽  
João Paulo Manfré Dos Santos ◽  
Amanda Rezende Mesquita ◽  
Thaynara Gonzaga Santos ◽  
...  
Keyword(s):  
Laser Therapy ◽  
Irradiation Time ◽  
Bacterial Species ◽  
Klebsiella Pneumonia ◽  
Stimulant Effect ◽  
Low Intensity ◽  
Low Intensity Laser Therapy ◽  
Intensity Laser ◽  
Group 1

Introduction: Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Objective: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 Enterococcus plates) and gram negative bacterias (3 Escherichia coli, 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans. Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A1, A2/B, B1, B2/C, C1, C2), and points A/B/C reciebed only one application, points A1,B1,C1 received 2 applications and points A2,B2,C2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm² dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. Results: In all the groups, no zone of inhibition nor growth were observed, indicating no bactericidal/fungicidal and/or bactericidal effect, in other words, in all groups and locations with different time exposures (A - 30 mW, B - and C 60 mW - 100 mW) the growth of bacteria and fungi were equal. Conclusion: Low intensity laser therapy (808nm and 660nm) did not produce bactericidal and/or bacteriostatic effects, and even no bio-stimulant effect on the bacteria.

scholarly journals Low intensity laser irradiation in myogenic precursor cells: an in vitro study (732.4)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
André Amaral ◽  
Heloisa Araújo ◽  
Nivaldo Parizotto ◽  
Tânia Salvini
Keyword(s):  
Laser Irradiation ◽  
In Vitro Study ◽  
Precursor Cells ◽  
Vitro Study ◽  
Low Intensity ◽  
Intensity Laser ◽  
Myogenic Precursor

MORPHOFUNCTIONAL PARAMETERS OF CATTLE ERYTHROCYTES UNDER STRESS AND ITS CORRECTION BY LOW-INTENSITY LASER RADIATION

1970 ◽  
pp. 67-71
Author(s):  
М. N. Ivashchenko ◽  
А. V. Deryugina ◽  
P. S. Ignatiev ◽  
V. B. Metelin ◽  
М. N. Talamanova ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Erythrocyte Membrane ◽  
Control Level ◽  
Experimental Studies ◽  
Erythrocyte Membranes ◽  
Physical Factors ◽  
Low Intensity ◽  
Intensity Laser ◽  
Technological Stress

The study investigated the content of malondialdehyde (MDA), the electrophoretic mobility of erythrocytes (EPME), the spectrum of erythrocyte membrane proteins and the morphology of cattle erythrocytes in in vitro experiments under stress and exposure to low-intensity laser radiation (LLLT). Clinical and experimental studies carried out in the last decade indicate the possibility of modulating the organism adaptive reactions when exposed to such physical factors as low-intensity laser radiation. The work showed that the effect of LLLT on the blood of stressed animals caused the restoration of the studied parameters to the level of physiological norms, while in animals that underwent technological stress, EPME was reduced by 31%, MDA concentration was increased by 65%. The effect of LLLT on the blood of unstressed animals did not lead to a change in EPME and MDA concentration. The study of the protein spectrum of erythrocyte membrane of animals subjected to technological stress revealed that the content of spectrin decreased by 16%, glycophorin C increased by 35%, the morphology of erythrocytes after stress was characterized by an increase in the number of echinocytes, stomatocytes and degeneratively altered erythrocytes. Under the LLLT action on the cow erythrocytes after stress there was a restoration of the morphology of cells and the content of proteins of erythrocyte membranes to the control level.

Methods for improving the functional and metabolic status of spermatozoa with a low-intensity laser in vitro

2020 ◽  
pp. 24-31
Author(s):  
A. A. Khisamova
Keyword(s):  
Seminal Fluid ◽  
Physiological Activity ◽  
Radiant Energy ◽  
Pulse Generation ◽  
Metabolic Status ◽  
Healthy Human ◽  
Low Intensity ◽  
Sperm Suspension ◽  
Intensity Laser

A method for improving the functional and metabolic status of spermatozoa, including increased motion and physiological activity of spermatozoa obtained from the seminal fluid of a healthy human in vitro and including the red wavelength radiation of a low-intensity laser, is characterized in that spermatozoa isolated from a healthy donor are washed with saline twice by centrifugation for 10 minutes at 1500 rpm and the sperm suspension is impacted on with a 632 nm semiconductor laser in the mode of variable pulse generation, with the pulse width of 2 ns, the frequency of 100 Hz, the radiant energy density of 0.56 j/cm2 , the exposure time of 1 minute, expressed in increased motion and physiological activity of spermatozoa.

Intracellular Calcium Ions Modulate Permeability of the Peritoneal Mesothelium In Vitro

1985 ◽  
Vol 5 (2) ◽  
pp. 105-108 ◽  
Author(s):  
Andrzej Breborowicz ◽  
Jan Knapowski ◽  
Grzegorz Breborowicz
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Local Anesthetic ◽  
Calcium Ions ◽  
Calcium Transport ◽  
Calcium Ionophore ◽  
Ionophore A23187 ◽  
Peritoneal Mesothelium ◽  
Vitro Effect

The authors studied the in vitro effect of a calcium-channel-blocker -verapamil, local anesthetic -bupivacaine and a calcium ionophore -A23187 on the permeability of the monolayer mesothelium from the rabbit's mesentery. Verapamil and bupivacaine increased transmesothelial flux of calcium while A23187 increased calcium transport only transiently. Verapamil augmented the permeability of the mesothelium to inulin and urea. However A23187 decreased the transmesothelial flux of inulin only whereas it increased the transport of urea. From this study we have concluded, that intracellular calcium may determine the permeability of the peritoneal mesothelium. Various important biological processes like muscle contraction, secretion of transmitters and hormones, and control of epithelial permability seem to be dependent on intracellular calcium-ion activity (1–4). Our previous paper suggested that a local anesthetic, bupivacaine, produces its effect on the peritoneal mesothelium through interaction with the cytoskeleton of the epithelial cells, and with the mem brane's calcium transport (5). In addition Palant et al found that the permeability of another leaky epithelium, that from the Necturus gallbladder, depends on the extracellular calcium concentrations and may be altered by drugs, which interfere with the entrance of these ions into the epithelial cells (6). Therefore, we decided to study the role that calcium ions may play in the transport of solutes across the peritoneal mesothelium in vitro.

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