scholarly journals Diet of Exotic Pirapitinga Piaractus brachypomus (Cuvier, 1818) From Vembanad-Kole Wetland, India, as Inferred From Gut Content Analysis and DNA Barcoding

2021 ◽  
Vol 34 (1) ◽  
Author(s):  
VINOD SREEDEVI ANUPAMA ◽  
SMRITHY RAJ ◽  
SUVARNA S. DEVI ◽  
APPUKUTTANNAIR BIJU KUMAR
Keyword(s):  
Dna Barcoding ◽  
Amazon Basin ◽  
Food Item ◽  
Invasion Biology ◽  
Flood Plain ◽  
Volume Index ◽  
Gut Contents ◽  
Diet Shift ◽  
Plant Matter ◽  
Number Percentage

Gut contents of the exotic characid fish Piaractus brachypomus (Cuvier, 1818) that escaped into the Vembanad-Kole Wetland, India, during the floods were examined for their gut food spectrum. The qualitative analysis of gut contents showed that the fish is an omnivore with detritus (27 %) as the most dominant food item followed by, plant matter (25 %), crabs (16 %), molluscs (12 %), fish (11 %) and insects (7 %), respectively. DNA barcoding of the gut contents revealed taxa such as Puntius mahecola (Valenciennes, 1844) (Cyprinid fish), Bellamaya sp. (Mollusca, Gastropoda, Viviparidae), Spiralothelphusa sp. (Crustacea, Brachyura, Gecarcinucidae) and Ictinogomphus sp. (Insecta, Odonata, Gomphidae) among diet contents. Ontogenic diet shift was not recorded, and none of the fishes showed empty guts, indicating the higher feeding rate and abundance of food in the habitat. The most predominant food item of P. brachypomus in the Vembanad-Kol wetland system is crabs in terms of percentage occurrence, percentage number, percentage volume, index of preponderance, and index of relative importance. Piaractus brachypomus showed greater variations in diet spectrum from their frugivorous nature in the home range (Amazon basin) to a more generalist heterogeneous feeding nature in the introduced ecosystem. The study found that in a highly biodiverse ecosystem, the introduced alien fish may compete with native fish and feed on native organisms. The paper suggests a precautionary approach in flood plain aquaculture, especially with the increase in extreme climatic events and holistic studies on invasion biology to manage invasive species.

scholarly journals Second floor, please: the fish fauna of floating litter banks in Amazonian streams and rivers

2013 ◽  
Vol 11 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Lucelia Nobre Carvalho ◽  
Luana Fidelis ◽  
Rafael Arruda ◽  
Andre Galuch ◽  
Jansen Zuanon
Keyword(s):  
Species Richness ◽  
Fish Assemblage ◽  
Amazon Basin ◽  
Stream Water ◽  
Fish Fauna ◽  
Assemblage Composition ◽  
Plant Matter ◽  
Carnivorous Species ◽  
Ephemeral Habitat ◽  
Dead Plant

Floating litter banks are an ephemeral habitat consisting of branches, twigs, flowers, seeds, and fruits that are trapped on the stream water surface by a variety of retention mechanisms. These heterogeneous materials form a deep layer of dead plant matter that is colonized by a variety of organisms, including fish that forage on the aquatic macroinvertebrates found in this unique habitat. In this study, we aimed to characterize which fish species occupy the floating litter banks and their trophic characteristics, as well as determine if fish assemblage composition and species richness can be predicted by the size of the floating litter banks. Fish sampling was conducted in five rivers located in the Amazon basin. Of the 31 floating litter banks sampled that contained fish, 455 individuals were recorded and were distributed within 40 species, 15 families and five orders. Siluriformes were the most representative order among the samples and contained the largest number of families and species. The fish fauna sampled was mainly composed of carnivorous species that are typically found in submerged litter banks of Amazonian streams. The fish assemblage composition in the kinon can be predicted by the volume of the floating litter banks using both presence/absence and abundance data, but not its species richness. In conclusion, kinon banks harbor a rich fish assemblage that utilizes this habitat for shelter and feeding, and may function as a refuge for the fishes during the peak of the flooding season.

Food of the Black Duck and Grey Teal in Coastal Northern New South Wales.

1979 ◽  
Vol 6 (3) ◽  
pp. 319 ◽  
Author(s):  
GN Goodrick
Keyword(s):  
New South ◽  
New South Wales ◽  
Flood Plain ◽  
Gut Contents ◽  
Terrestrial Invertebrates ◽  
Food Items ◽  
South Wales ◽  
Water Beetle ◽  
Black Duck ◽  
Maize Grain

The study area of 500 km2 was humid and subtropical, on flood plain near Coraki, New South Wales. Gut contents were examined and the percentage by volume of different food items is tabulated for black duck (Anas superciliosa) and grey teal (A. gibberifrons). Black duck fed on flooded meadow-grass flats in autumn, moving when the flats dried to seasonal swamps, and when those also dried, to lagoons. Diet varied with place. Seeds of grasses, swamp plants and lagoon plants were eaten, with water snails and water beetle adults and larvae, water spiders and ostracods, and terrestrial invertebrates being eaten when the dry flats became flooded. They ate also waste maize grain from harvested fields before ploughing. Grey teal fed in the seasonal swamps in winter and then left the area almost completely; they ate almost the same items as black duck but fed by the muddy edges of seasonal swamps more than the black duck did. It was known that black duck strip seed from the growing plant and grey teal pick up the fallen seed.

scholarly journals Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

2021 ◽  
Vol 19 (2) ◽  
Author(s):  
Karen L. A. Guimarães ◽  
Juan J. Rosso ◽  
Mendelsohn F. B. Souza ◽  
Juan M. Díaz de Astarloa ◽  
Luís R. R. Rodrigues
Keyword(s):  
Dna Barcoding ◽  
Amazon Basin ◽  
First Record ◽  
Morphological Data ◽  
Disjunct Distribution ◽  
Valid Species ◽  
Integrative Approach ◽  
Molecular Evidence ◽  
La Plata ◽  
La Plata Basin

Abstract The Hoplias malabaricus group encompasses six valid species and still is believed to harbors cryptic diversity. In this work, an integrative approach including morphological, DNA barcoding, and cytogenetic considerations was conducted to characterize a population of H. malabaricus from the Amazon basin that was recently allocated in the same mitochondrial lineage with H. misionera, a species originally described from La Plata basin. The DNA barcoding analysis revealed that the Amazon population nested together with H. misionera specimens from the La Plata basin (BIN AAB1732) in the same cluster. The intragroup distance (0.5%) was 12 times lower than the nearest neighbor (6%) distance. The morphometric analysis demonstrated slightly variation between Amazon and La Plata populations, being the former composed by larger specimens. Further morphological data supported the molecular evidence of H. misionera inhabiting Amazon basin. The karyotype characterization of H. misionera in the Amazon population showed 2n=40 and karyotypic formulae 20m+20sm, that added to C-banding, Ag-NOR and 18S results are suggestive of the similarity to karyomorph C of H. malabaricus. This work reveals the first record of H. misionera outside of La Plata basin and expands the species distribution for 2500 km northward until the Marajó Island, estuary of Amazonas River.

scholarly journals Variability in the diet diversity of catfish highlighted through DNA barcoding

2020 ◽  
Author(s):  
Chinnamani Prasannakumar ◽  
Gunasekaran Iyyapparajanarasimapallavan ◽  
M. Ashiq Ur Rahman ◽  
P. Mohanchander ◽  
T. Sudhakar ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Monsoon Season ◽  
Stomach Contents ◽  
Hermit Crabs ◽  
Gut Contents ◽  
Reference Database ◽  
Perna Viridis ◽  
Fish Diet ◽  
Diet Diversity ◽  
Post Monsoon

AbstractIdentification and quantification of fish diet diversity was the first step in understanding the food web dynamics and ecosystem energetics, where the contribution of DNA barcoding technique has been important. We used DNA barcoding to identify the stomach contents of a euryhaline, benthophagous catfish Ariius maculatus. From 40 catfish stomach items sampled in two different seasons, we barcoded 67 piscine and macro-invertebrates prey items, identified as belonging to 13 species in 4 major phyla (viz., Chordate, Arthropod, Annelida and Mollusca). It is important to note that the mollusc taxa (Meritrix meritrix and Perna viridis) and a species of fish (Stolephorus indicus) could not be found among the gut contents of A. maculatus sampled during the pre- and post-monsoon season, respectively. Among the piscine diets of A. maculatus, Eubleekeria splendens (23.5%) and Stolephorus indicus (23.5%) were the major prey taxa during pre-monsoon season. The hermit crabs forms the major constituents of both pre- and post-monsoon seasons, among invertebrate taxa. Polychaete, Capitella capitata (25.92%) was abundantly consumed invertebrates next to hermit crabs. We noticed that in pre-monsoon A. maculatus was more piscivorous than post-monsoon. As revealed through Kimura-2 parametric pair­wise distance analysis, the diet diversity was relatively higher in post-monsoon. The accumulation curve estimated 57 haplotypes within 14 barcoded species (including the host A. maculatus). Majority of haplotypes were found among fishes (47.36%) followed by Arthropods (28.07%), Annelids (14.03%) and Mollusca (10.52%), respectively. This study also highlights that there is a growing concern about A. maculatus’s aggressive predation on commercially important stocks of fish and invertebrates. We will continue to expand the coverage of species barcoded in the reference database, which will become more significant as meta- and environmental DNA barcoding techniques become cheaper and prevalent.

scholarly journals Pretreatment Method for DNA Barcoding to Analyze Gut Contents of Rotifers

2020 ◽  
Vol 10 (3) ◽  
pp. 1064 ◽  
Author(s):  
Hye-Ji Oh ◽  
Paul Henning Krogh ◽  
Hyun-Gi Jeong ◽  
Gea-Jae Joo ◽  
Ihn-Sil Kwak ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Analysis ◽  
Extracellular Dna ◽  
Food Sources ◽  
Gut Contents ◽  
Gut Content ◽  
Reservoir Water ◽  
Pretreatment Method ◽  
Analysis Techniques ◽  
Rotifer Species

We designed an experiment to analyze the gut content of Rotifera based on DNA barcoding and tested it on Asplanchna sp. in order to ensure that the DNA extracted from the rotifer species is from the food sources within the gut. We selected ethanol fixation (60%) to minimize the inflow effects of treated chemicals, and commercial bleach (the final concentration of 2.5%, for 210 s) to eliminate the extracellular DNA without damage to the lorica. Rotifers have different lorica structures and thicknesses. Therefore, we chose a pretreatment method based on Asplanchna sp., which is known to have weak durability. When we used the determined method on a reservoir water sample, we confirmed that the DNA fragments of Chlorophyceae, Diatomea, Cyanobacteria, and Ciliophora were removed. Given this result, Diatomea and cyanobacteria, detected from Asplanchna, can be considered as gut contents. However, bacteria were not removed by bleach, thus there was still insufficient information. Since the results of applying commercial bleach to rotifer species confirmed that pretreatment worked effectively for some species of rotifers food sources, in further studies, it is believed to be applicable to the gut contents analysis of more diverse rotifers species and better DNA analysis techniques by supplementing more rigorous limitations.

scholarly journals Evidence of terrestrial feeding in the arboreal lizard Enyalius bilineatus (Sauria, Polychrotidae) of south-eastern Brazil

2001 ◽  
Vol 61 (1) ◽  
pp. 91-94 ◽  
Author(s):  
C. ZAMPROGNO ◽  
M. das G. F. ZAMPROGNO ◽  
R. L. TEIXEIRA
Keyword(s):  
Strong Evidence ◽  
Food Item ◽  
Prey Type ◽  
Foraging Strategy ◽  
Gut Contents ◽  
Important Food ◽  
Food Items ◽  
South Eastern ◽  
Eastern Brazil ◽  
Important Food Item

The diet of the arboreal lizard E. bilineatus was assessed based on analysis of gut contents of specimens collected in the remnants of the Atlantic rainforest in Espírito Santo State, south-eastern Brazil. Stomachs of the lizards examined (SVL = 57.0-85.0 mm) contained nine arthropod orders. E. bilineatus utilizes a broad range of arthopodan prey type and sizes, and can be considered an arthropod generalist, which uses a sit-and-wait foraging strategy. Orthoptera represented the most important food item, followed by Dictyoptera and Hymenoptera. Hemiptera, Homoptera, Lepidoptera (larvae), Coleoptera, Araneae, and Diplopoda constituted the remainder of food items. The presence of Diplopoda, which are not generally found on tree trunks, provides particularly strong evidence the E. bilineatus forages in leaf litter on the ground.

scholarly journals Ecology and life history of an Amazon floodplain cichlid: the discus fish Symphysodon (Perciformes: Cichlidae)

2008 ◽  
Vol 6 (4) ◽  
pp. 599-612 ◽  
Author(s):  
William G. R. Crampton
Keyword(s):  
Amazon Basin ◽  
High Water ◽  
Organic Detritus ◽  
Plant Matter ◽  
Amazon Floodplain ◽  
Flood Level ◽  
Flooded Forests ◽  
History Of ◽  
Eggs And Larvae ◽  
Fine Organic

The discus fishes of the genus Symphysodon are popular ornamental cichlids that occur in floodplain lakes and flooded forests of the lowland Amazon Basin. These habitats are characterized by extreme seasonal fluctuations in the availability of food, shelter and dissolved oxygen, and also the densities of predators and parasites. Most aspects of discus biology are influenced by these fluctuating conditions. This paper reports an autoecological study of the western Amazonian discus S. haraldi (until recently classified as S. aequifasciatus). This species feeds predominantly on algal periphyton, fine organic detritus, plant matter, and small aquatic invertebrates. At high water it forages alone or in small groups in flooded forests. At low water it forms large aggregations in fallen tree crowns along lake margins. Breeding occurs at the beginning of the flood season, ensuring that the progeny are well grown before the next low water period. Symphysodon haraldi is an iteroparous partial spawner, reaches reproductive maturity within a year, and undertakes parental care of its eggs and larvae. The timing of spawning events, and/or the rate of brood survival, may be influenced by fluctuations in the flood level, resulting in a non-unimodal distribution of size classes for the subsequent 1+ cohort.

scholarly journals On the natural diet of Daphnia laevis in the eutrophic Pampulha reservoir (Belo Horizonte, Minas Gerais)

2002 ◽  
Vol 62 (3) ◽  
pp. 445-452 ◽  
Author(s):  
E. M. ESKINAZI-SANT'ANNA ◽  
P. M. MAIA-BARBOSA ◽  
F. A. R. BARBOSA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Food Item ◽  
Inorganic Material ◽  
Minas Gerais ◽  
Gut Contents ◽  
Gut Content ◽  
Natural Diet ◽  
Belo Horizonte ◽  
Scanning Electron

The aim of this study was to assess the major food items ingested by adult specimens of Daphnia laevis within the eutrophic Pampulha reservoir in Belo Horizonte, Minas Gerais, Brazil. The gut content was analyzed after addition of sodium hypochlorite and also through the examination of dissected guts under scanning electron microscopy. The results showed that Chlorophyceae was the main food item ingested, representing c. 80.5% of the total ingested food. Moreover, Eutetramorus fottii, Coelastrum pseudomicroporum and Oocystis lacustris, the dominant phytoplankton species within the reservoir, were the most frequent cells found in the gut contents. Euglenophyta also represented an important food item accounting for 15% of the ingested material, including mainly Trachelomonas volvocina and Euglena oxyuris, although less abundant in the reservoir (< 10% of total phytoplankton). Blue-green algae occurred at much lower percentages in the guts than in the phytoplankton. A small amount of undigested Microcystis aeruginosa colonies were also found in the gut content of D. laevis. Scanning electron microscopy results showed that, besides phytoplankton cells, a great amount of abiogenic material was also ingested. The amount of this inorganic material increased considerably in the tract (from 15% to 75% of the gut content), when a peak of D. laevis was observed in the reservoir. Our assumption is that the ingestion of this inorganic material can be a strategy used by D. laevis to obtain additional food supply.

scholarly journals Forensic botany and forensic chemistry working together: application of plant DNA barcoding as a complement to forensic chemistry—a case study in Brazil

Genome ◽  
2019 ◽  
Vol 62 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Renato T.F. Paranaiba ◽  
Carlos B.V. Carvalho ◽  
Jorge M. Freitas ◽  
Levy H. Fassio ◽  
Élvio D. Botelho ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Plant Species ◽  
Plant Material ◽  
Forensic Analysis ◽  
Forensic Chemistry ◽  
Artemisia Absinthium ◽  
Genetic Sequencing ◽  
Plant Dna ◽  
Plant Matter ◽  
Working Together

Recently, Brazilian Federal Police used forensic chemistry and forensic botany techniques on a case. Two packets containing fragmented plant matter were seized and sent for forensic analysis. Forensic chemistry, the gold standard for evaluating plant material suspected to contain illicit substances, did not find illicit materials. Gas chromatography coupled mass spectrometry (GC-MS) identified thujone in the botanical material. Thujone is a chemical compound naturally found in many plant species, notably Artemisia absinthium. Because doubt remained, we next used plant DNA barcoding methods. Total DNA from plant tissue fragments was extracted and five different DNA regions were amplified, sequenced, and analyzed using plant DNA barcoding methods. Genetic analysis yielded 30 good quality sequences representing five taxa. Most specimens were identified as A. absinthium. Few studies focus on practical forensic applications of plant DNA barcoding methods using a case solved in a forensic laboratory with its difficulties and limitations. To the best of our knowledge, this is the first study to report an effective joint effort of forensic chemistry and botany techniques to assess plant material in Brazil. The availability of a new technical approach for the genetic sequencing of plant species will enhance many forensic investigations and inspire similar initiatives.

scholarly journals Diet and functional feeding groups of Chironomidae (Diptera) in the Middle Paraná River floodplain (Argentina)

2012 ◽  
Vol 102 (2) ◽  
pp. 117-121 ◽  
Author(s):  
M. Celeste Galizzi ◽  
Florencia Zilli ◽  
Mercedes Marchese
Keyword(s):  
Food Item ◽  
Niche Overlap ◽  
Paraná River ◽  
Gut Contents ◽  
Functional Feeding Groups ◽  
River Floodplain ◽  
Feeding Groups ◽  
Parana River ◽  
Paraná River Floodplain ◽  
Middle Paraná

The gut contents of nine genera of benthic Chironominae and Tanypodinae from the Middle Paraná River floodplain habitats (a lake and a secondary channel) were analyzed to determine their feeding patterns and functional feeding groups. Amorphous detritus, animal and vegetal tissues, and mineral materials (predominantly sand) were observed in the larval guts. Amorphous detritus were the main food item found for Polypedilum (Tripodura) sp., Chironomus gr. decorus sp., Endotribelos sp., Phaenopsectra sp., Cladopelma sp., and Pelomus sp. (Chironominae), while animal tissues (mainly oligochaetes) were the most important food item found for Ablabesmyia (Karelia) sp., Coelotanypus sp., and Procladius sp. (Tanypodinae). Dietary overlap was calculated for all pairs of genera. Within predators, the highest overlap was obtained between Coelotanypus sp. and Ablabesmyia (Karelia) sp., while within detritivores the highest niche overlap was obtained between Endotribelos sp. and Phaenopsectra sp.

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