Regeneration of Dead Bougainvillea Tree with Organic Manure

Bougainvillea is hard, woody climber tree, grow in high salt tolerant soil. The present study was carried out in regenerating Bougainvillea plant with organic manure. The research work was conducted at kitchen garden campus in January 2020. The collected soil samples of five trees species namely Pimple, Neem, Khejari and Rohira are mixed with 10kg fresh cow dung, 5kg cow urine, 2kg molasses & 2kg flour Kitchen wastes 10kg, Charcoal 10kg, Molasses 2kg, Rice 1kg, Humus 10kg, Wheat 10kg, Crashed sugar cane 10kg, Chicken manures 2kg, Wooden saw dust, Wooden chips & Rice lusts and mixed with water for preparing organic product. The organic product keeps for 3 days in open conditions. The prepared organic product was poured into the shoot and root. Later, the regrowth of the shoot and the root were reported in 4-5 months. The organic product enhanced the metabolism for regenerating permanent tissue and Meristematic tissue of Shoot horizon and root horizon. Later, The lateral branches and flower were emerged from the dead plant. The formulated organic product is competent to regrow dead plant. Keywords: Bougainvillea, Dead plant, organic manure, regeneration, soil, climate

Determination of the species of fungi of the genus Fusarium by molecular method

Currently, more than 10,000 species of fungi associated with plants have been found, and it is not surprising that fungal diseases cause a lot of harm. Most species of the genus Fusarium are soil protrophs that live on dead plant remains, in the rhizosphere of plants, on the surface of roots. They cause rot of roots, seeds, fruits, tubers, root crops. All forms of fusariums progress at high temperatures and humidity. The analysis of literary sources and experimental data allowed us to formulate the purpose of our research: “To analyze the species diversity of fungi of the genus Fusarium from the soil, table carrot plants, depending on the ecological and geographical zones of growth.” As a result, using PCR analysis, 13 samples attributed to F. langsethiae were identified; 2 samples of F. ochusrohim; 8 samples of F. roae; 2 of F. sporotrichioides; 1 sample attributed to F. sulmorum, this sample was collected in the Rostov region and the Moscow region. It was also revealed that the diversity of soil and climatic conditions in different zones leads to a change in the species composition of representatives of R. Fusarium: the species composition of fungi of R. Fusarium varies significantly depending on agro-climatic conditions. In wetter and warmer conditions (southern regions), specific species of F. culmorum, F. sporotrichiella, and F. oxysporum dominate. F. heterosporum, F. nivale are less common; F. graminearum species lives on plant residues, on plant roots or in the rhizosphere zone, but practically does not occur in the soil in its pure form. Thus, this direction is promising for the study of fungi of the genus Fusarium, since this microorganism lives in the soil and on plants. It is advisable to identify the pathogen from samples from different regions to significantly increas the representativeness of samples within the regions with further identification of diverse mycelium, thereby creating a scale of diverse mycelium.

Estimasi Karbon Tersimpan pada Nekromassa Tumbuhan di Rawa Lebak Kecamatan Martapura, Kalimantan Selatan

South Kalimantan is one of carbon contributor with an area of swamp with ± 1,140,207 ha area of swamp land. The potential area for changed to be an agricultural land is ± 763,207 ha, and the remain used for pool when the rainy season is come. The highest C reserve is in biomass (mass of living-plant part) and necromass (mass of dead-plant part) at the top soil, microbe, and soil-organic matter. Based on description above, the problem is how much stored-carbon in necromass of plant at martapura lowland swamp, because the largest carbon storage found in necromass of plant. The purpose of this study was to estimate the stored carbon contained in necromass of vegetation in lowland swamp. This research has been done in Martapura from April to July 2009. Sampling is done at 4 location include Tungkaran village, Keramat Baru village, Sungai Rangas village and Sungai Tabuk village. Each sampling location divided into 2 stations in one sampling. Analysis of stored-carbon in necromass of plant is using Walkey and Black Method. The result showed that average ranges of carbon stored in plant necromass are 490,95 – 1744,66 gm-2.

Secretome Analysis Identified Extracellular Superoxide Dismutase and Catalase of Macrophomina Phaseolina: It’s Production and Properties

Macrophomina phaseolina, a necrotrophic fungal pathogen is known to cause charcoal rot disease in food crops, pulse crops, oil crops and cotton and fibre crops. Necrotrophic fungi survive on dead plant tissue. It is well known that reactive oxygen species (ROS) are produced by host plant during plant pathogen interaction. However, it is still unclear how M. phaseolina can overcome the ROS induced cellular damage. To mimic the invasion of M. phaseolina inside the plant cell wall, we developed solid substrate fermentation where M. phaseolina spore suspension was inoculated on wheat bran bed and incubated for vegetative growth. To analyse the secretome of M. phaseolina after different day interval, its secretory material was collected and concentrated. Both superoxide dismutase (SOD) and catalase were detected in the secretome by zymogram. The presence of SOD and catalase was further confirmed by liquid chromatography based mass spectrometry. The physicochemical properties of M. phaseolina catalase in terms of stability towards pH, temperature, metal ions and chaotropic agent and inhibitors indicated its fitness at different environmental conditions. Apart from the production of catalase in SSF, the studies on this particular microorganism may also have significance in necrotrophic fungal pathogen and their susceptible host plant interaction.

Gyrothrix sagarensis SP. NOV.: A NOVEL HYPHOMYCETOUS FUNGUS ON MEDICINAL PLANT Gardenia latifolia AITON FROM CENTRAL INDIA

Gardenia latifolia is commonly known as Indian boxwood or Ceylon boxwood. Various parts of this plant are utilized to treat several cases of inflammatory pain, skin diseases, caries in humans, snake bite, stomachache, haemorrhage along with the ephemeral fever in live stocks. During the routine survey of Dr. Harisingh Gour Vishwavidyalaya Campus, Sagar for mycotaxonomic evaluation of terrestrial plants an interesting fungal specimen was encountered on G. latifolia Aiton which upon detailed morphological observations and mycotaxonomic treatment, proved to be a novel fungal species Gyrothrix sagarensis. It is also noteworthy that most of the species of this genus Gyrothrix (Corda) Corda, are reported on dead plant parts while the present novel species was collected and examined on living plant parts (i.e. on twig) of G. latifolia.

Assessment of Leaf Litter Production in Tropical Trees

Litter is the layer of detached dead plant material present on the surface of the soil. It plays an imperative part in the nutrient budgeting in agroforestry system. The quantum of litter produced varies with species and also seasons. Generally the litter production will be more during summer and less during monsoon times. Further the nature of the tree species like evergreen and deciduous will also influence litter production. In the present study, 10 tree species commonly found in farm lands were identified and selected from the experimental plot, which was located in Southern agro-climatic zone of Tamil Nadu and the soil type is alfisol with a pH of 6.0. The age of the trees was 10 years. The litter collection was done throughout the year on monthly basis and quantities produced were recorded. The data recorded were subjected to statistical analysis. The results revealed the following. Acacia auriculiformis produced a maximum leaf litter of 10.0 t/ha/year followed by Senna siamea. Summer season had more litter (3.55 kg/tree) followed by winter (1.54 kg/ha). A maximum mean monthly litter of 2.54 kg/tree was recorded in Tamarindus indica followed by Acacia mangium (0.76 kg/tree). Senna siamea and Gliricidia produced leaf litter throughout the year. Tamarind produced a maximum annual litter of 30.42 kg/tree followed by Acacia mangium (9.07 kg/tree).

Poly- and Monoamine Metabolism in Streptomyces coelicolor: The New Role of Glutamine Synthetase-Like Enzymes in the Survival under Environmental Stress

Soil bacteria from the genus <i>Streptomyces</i>, phylum Actinobacteria, feature a complex metabolism and diverse adaptations to environmental stress. These characteristics are consequences of variable nutrition availability in the soil and allow survival under changing nitrogen conditions. <i>Streptomyces coelicolor</i> is a model organism for Actinobacteria and is able to use nitrogen from a variety of sources including unusual compounds originating from the decomposition of dead plant and animal material, such as polyamines or monoamines (like ethanolamine). Assimilation of nitrogen from these sources in <i>S. coelicolor</i> remains largely unstudied. Using microbiological, biochemical and in silico approaches, it was recently possible to postulate polyamine and monoamine (ethanolamine) utilization pathways in <i>S. coelicolor.</i> Glutamine synthetase-like enzymes (GS-like) play a central role in these pathways. Extensive studies have revealed that these enzymes are able to detoxify polyamines or monoamines and allow the survival of <i>S. coelicolor</i> in soil containing an excess of these compounds. On the other hand, at low concentrations, polyamines and monoamines can be utilized as nitrogen and carbon sources. It has been demonstrated that the first step in poly-/monoamine assimilation is catalyzed by GlnA3 (a γ-glutamylpolyamine synthetase) and GlnA4 (a γ-glutamylethanolamide synthetase), respectively. First insights into the regulation of polyamine and ethanolamine metabolism have revealed that the expression of the <i>glnA3</i> and the <i>glnA4</i> gene are controlled on the transcriptional level.

Integration of chemosensing and carbon catabolite repression impacts fungal enzyme regulation and plant associations

Fungal metabolism and enzyme production are regulated by nutrient availability and by interactions with the living environment. We investigated the mechanisms underpinning adaptation of the biotechnological fungus Trichoderma reesei to decaying plant biomass versus living plants. We found that concentration-gated response to glucose, the main molecule sensed from dead plant biomass, is mediated by a conserved signaling pathway downstream of G protein-coupled receptors (GPCRs), while the carbon catabolite repressor CRE1 is critical for glucose concentration gating. The GPCRs CSG1 and CSG2 are further required for root colonization and formation of appressorium like structures on plant surfaces. Acceleration of sexual development in the presence of plant roots and their interactions with fruiting bodies indicates preferential association with plants. Our results reveal a complex sensing network governing resource distribution, enzyme production and fungal development that explains previously observed phenomena in fermentations and opens new perspectives for industrial strain improvement and agriculture.

Analysis of Fire Catchers in Sago Palm Smallholding Plantations on Peatland in Tebing Tinggi Timur, Kepulauan Meranti District, Riau Province

Fire on peatlands takes place not only in areas where preparation for cultivation is in progress, but also in areas that have been cultivated, such as for sago palm growing. One of the important factors that influence the vulnerability of cultivated peatlands to fire is the availability and amount of biomass fuel that easily catches fire, such as ferns, grasses, and broadleaved shrubs in their living as well as dead (litter) stages. The present study concerns the vulnerability of peatland utilized for sago growing by smallholders, focused in Tebing Tinggi Timur, Kepulauan Meranti District, Riau Province. The objectives of this study were to analyze the composition and assess the biomass of fire catchers in the sago palm plantation. Data collection was conducted in three locations in Kepau Baru Village. In each location, a 1000 m-long transect line was drawn and 10 sampling plots of 2 m x 2 m were established along the line with 100 m intervals. Results showed that total biomass of fire catchers in this growing system was averaged at 24681 kg/ha, in which the dead plant fraction was predominating (average at 17478 kg/ha or 70.81%) over the living plant fraction (in average 7203 kg/ha or 29.19%). The latter consisted of ferns that averaged at 2.191 kg/ha or 30.42%, grasses (20 kg/ha or 0.28%), non-woody broadleaved shrubs (581 kg/ha or 8.07%), and woody broadleaved shrubs (4411 kg/ha or 61.24%). The amount of fire catchers in relatively large quantities in this plantation system could magnify fire risk during drier months, especially during El-nino years. Keywords: biomass, fire risk, grasses, non-woody broadleaved shrubs, woody broadleaved shrubs

Symbiont-Mediated Digestion of Plant Biomass in Fungus-Farming Insects

Feeding on living or dead plant material is widespread in insects. Seminal work on termites and aphids has provided profound insights into the critical nutritional role that microbes play in plant-feeding insects. Some ants, beetles, and termites, among others, have evolved the ability to use microbes to gain indirect access to plant substrate through the farming of a fungus on which they feed. Recent genomic studies, including studies of insect hosts and fungal and bacterial symbionts, as well as metagenomics and proteomics, have provided important insights into plant biomass digestion across insect–fungal mutualisms. Not only do advances in understanding of the divergent and complementary functions of complex symbionts reveal the mechanism of how these herbivorous insects catabolize plant biomass, but these symbionts also represent a promising reservoir for novel carbohydrate-active enzyme discovery, which is of considerable biotechnological interest.