Effect of mango seed kernel extract on polyphenol oxidase inhibition and shelf life of shrimp during iced storage

Background: Polyphenol oxidase (PPO) has applications in pharmaceutical, environmental, and food industries, however, conventional techniques for its purification are considered costly and laborious. Alternative methods of purification are needed. Methods: This study explored the use of an alcohol/salt-based aqueous two-phase system (ATPS) to partially purify PPO from mango seed kernel (MSK). Specifically, this study determined the effects of 1-propanol/potassium phosphate buffer mass ratio on the partition parameters such as partition coefficients, activity recovery, and purification fold. Results: The enzyme partition coefficient (K E ) was greater than 1, which signified that more PPO was partitioned in the top phase due to its hydrophobic nature. Specifically, the 3.22 1-propanol/PPB mass ratio had the highest K E of 1.91 ± 0.16 and 1.46 ± 0.16 for runs 1 and 2, respectively. Moreover, the same mass ratio led to the highest activity recovery in the top phase reaching up to 73.99 ± 1.56% for run 1 and 64.27 ± 2.48% for run 2. In terms of purification fold, runs 1 and 2 had PF values of 0.55 ± 0.05 and 0.70 ± 0.01, respectively. Since the values obtained were low due to the large number of contaminants extracted together with the target enzyme in the top phase, the use of a back-extraction step recovered the PPO in the bottom phase and resulted in an activity recovery of 82.94 ± 1.05% and a purification fold of 7.01 ± 0.95. Conclusion: Results have shown that varying the mass ratio had significant effects on the partition parameters.

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Characterization of starch from different non-traditional sources and its application as coating in ‘Palmer’ mango fruit

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044011220 ◽

2020 ◽

Vol 44 ◽

Author(s):

Antonio Augusto Marques Rodrigues ◽

Luana Ferreira dos Santos ◽

Rayssa Ribeiro da Costa ◽

Débora Tamara Félix ◽

José Henrique Bernardino Nascimento ◽

...

Keyword(s):

Water Solubility ◽

Amylose Content ◽

Starch Content ◽

Chemical Properties ◽

Seed Kernel ◽

Mango Fruit ◽

Mango Seed Kernel ◽

Randomized Experimental Design ◽

Mango Seed ◽

Biodegradable Coatings

ABSTRACT Yam, cassava, jackfruit seed and mango seed kernel have potential for the extraction and use as starch in the food industry of starch or for the formulation of biodegradable coatings. As a biodegradable coating, starch can be applied in fruits characterized by a fast maturation, such as mango, which requires technologies to increase its shelf life. The aim of this study was to characterize starch from four non-traditional sources and to evaluate their potential as coating for ‘Palmer’ mango fruit. Starches used were extracted from cassava, mango seed kernel, jackfruit seed, and yam, and had their physical, optical, and chemical properties characterized for later use as coatings of ‘Palmer’ mango fruit. Fruits were coated with 3% cassava starch, 3.5% jackfruit seed starch, 3.5% mango seed kernel starch and 3.5% yam starch, and were compared to the control (uncoated). They were then stored at 24.4 ± 0.3 °C and 87 ± 2% RH and evaluated for 12 days. A 5x7 factorial arrangement in a completely randomized experimental design was adopted. Total starch content was higher than 70% in the four sources of starch. Starches from jackfruit and yam had higher amylose content. The four sources of starch had low water solubility and swelling power, with jackfruit seed starch having the highest values. The coating sources were effective in maintaining quality, particularly mango seed kernel starch because it reduced respiratory rate and weight loss in 27.7% and 33.8%, respectively, as well as jackfruit seed starch as it delayed fruit skin yellowing.

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