Faculty Opinions recommendation of A dynamic spectrum of monocytes arising from the in situ reprogramming of CCR2+ monocytes at a site of sterile injury.

Author(s):  
Philippe Saas ◽  
Sylvain Perruche
Keyword(s):  
2015 ◽  
Vol 212 (4) ◽  
pp. 447-456 ◽  
Author(s):  
Daniela Dal-Secco ◽  
Jing Wang ◽  
Zhutian Zeng ◽  
Elzbieta Kolaczkowska ◽  
Connie H.Y. Wong ◽  
...  

Monocytes are recruited from the blood to sites of inflammation, where they contribute to wound healing and tissue repair. There are at least two subsets of monocytes: classical or proinflammatory (CCR2hiCX3CR1low) and nonclassical, patrolling, or alternative (CCR2lowCX3CR1hi) monocytes. Using spinning-disk confocal intravital microscopy and mice with fluorescent reporters for each of these subsets, we were able to track the dynamic spectrum of monocytes that enter a site of sterile hepatic injury in vivo. We observed that the CCR2hiCX3CR1low monocytes were recruited early and persisted for at least 48 h, forming a ringlike structure around the injured area. These monocytes transitioned, in situ, from CCR2hiCx3CR1low to CX3CR1hiCCR2low within the ringlike structure and then entered the injury site. This phenotypic conversion was essential for optimal repair. These results demonstrate a local, cytokine driven reprogramming of classic, proinflammatory monocytes into nonclassical or alternative monocytes to facilitate proper wound-healing.


Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 738
Author(s):  
Nicola Rossi ◽  
Mario Bačić ◽  
Meho Saša Kovačević ◽  
Lovorka Librić

The design code Eurocode 7 relies on semi-probabilistic calculation procedures, through utilization of the soil parameters obtained by in situ and laboratory tests, or by the means of transformation models. To reach a prescribed safety margin, the inherent soil parameter variability is accounted for through the application of partial factors to either soil parameters directly or to the resistance. However, considering several sources of geotechnical uncertainty, including the inherent soil variability, measurement error and transformation uncertainty, full probabilistic analyses should be implemented to directly consider the site-specific variability. This paper presents the procedure of developing fragility curves for levee slope stability and piping as failure mechanisms that lead to larger breaches, where a direct influence of the flood event intensity on the probability of failure is calculated. A range of fragility curve sets is presented, considering the variability of levee material properties and varying durations of the flood event, thus providing crucial insight into the vulnerability of the levee exposed to rising water levels. The procedure is applied to the River Drava levee, a site which has shown a continuous trend of increased water levels in recent years.


2017 ◽  
Vol 141 (10) ◽  
pp. 1313-1315 ◽  
Author(s):  
Reena Singh ◽  
Kathleen R. Cho

Context.— Nonuterine high-grade serous carcinomas (HGSCs) are believed to arise most often from precursors in the fallopian tube referred to as serous tubal intraepithelial carcinomas (STICs). A designation of tubal origin has been suggested for all cases of nonuterine HGSC if a STIC is identified. Objective.— To highlight that many different types of nongynecologic and gynecologic carcinomas, including HGSC, can metastasize to the tubal mucosa and mimic de novo STIC. Data Sources.— A mini-review of several recently published studies that collectively examine STIC-like lesions of the fallopian tube. Conclusions.— The fallopian tube mucosa can be a site of metastasis from carcinomas arising elsewhere, and pathologists should exercise caution in diagnosing STIC without first considering the possibility of metastasis. Routinely used immunohistochemical stains can often be used to determine if a STIC-like lesion is tubal or nongynecologic in origin. In the context of uterine and nonuterine HGSC, STIC may represent a metastasis rather than the site of origin, particularly when widespread disease is present.


2020 ◽  
Author(s):  
Yiran Tong ◽  
Matthew J. Berens ◽  
Bridget A. Ulrich ◽  
Jakov Bolotin ◽  
Jennifer H. Strehlau ◽  
...  

This work determines isotope enrichment factors for RDX during reduction by iron minerals. The values determined are used to assess the transformation of RDX at a site with groundwater contamination and in laboratory column reactors simulating in situ chemical reduction treatment.


2013 ◽  
Vol 68 (8) ◽  
pp. 1770-1777 ◽  
Author(s):  
M. Faleschini ◽  
J. L. Esteves

The study of benthic metabolism is an interesting tool to understand the process that occurs in bottom water at wastewater stabilization ponds. Here, rates of benthic oxygen consumption and nutrient exchange across the water–sludge interface were measured in situ using a benthic chamber. The research was carried out during autumn, winter, and summer at a municipal facultative stabilization pond working in a temperate region (Puerto Madryn city, Argentina). Both a site near the raw wastewater inlet (Inlet station) and a site near the outlet (Outlet station) were sampled. Important seasonal and spatial patterns were identified as being related to benthic fluxes. Ammonium release ranged from undetectable (autumn/summer – Inlet station) to +30.7 kg-NH4+ ha−1 d−1 (autumn – Outlet station), denitrification ranged from undetectable (winter – in both sites) to −4.0 kg-NO3− ha−1 d−1 (autumn – Outlet station), and oxygen consumption ranged from 0.07 kg-O2ha−1 d−1 (autumn/summer – Outlet station) to 0.84 kg-O2ha−1 d−1 (autumn – Inlet station). During the warmer months, the mineralization of organic matter from the bottom pond acts as a source of nutrients, which seem to support the important development of phytoplankton and nitrification activity recorded in the surface water. Bottom processes could be related to the advanced degree and efficiency of the treatment, the temperature, and probably the strong and frequent wind present in the region.


2000 ◽  
Vol 64 (2) ◽  
pp. 255-266 ◽  
Author(s):  
J. J. Reece ◽  
S. A. T. Redfern ◽  
M. D. Welch ◽  
C. M. B. Henderson

AbstractThe crystal structure of a manganoan cummingtonite, composition [M4](Na0.13Ca0.41Mg0.46Mn1.00) [M1,2,3](Mg4.87Mn0.13)(Si8O22)(OH)2, (Z = 2), a = 9.5539(2) Å, b = 18.0293(3) Å, c = 5.2999(1) Å, β = 102.614(2)° from Talcville, New York, has been refined at high temperature using in situ neutron powder diffraction. The P21/m to C2/m phase transition, observed as spontaneous strains +ε1 = −ε2, occurs at ˜107°C. Long-range disordering between Mg2+ and Mn2+ on the M(4) and M(2) sites occurs above 550°C. Mn2+ occupies the M(4) and M(2) sites preferring M(4) with a site-preference energy of 24.6±1.5 kJ mol−1. Disordering induces an increase in XMnM2 and decrease in XMnM4 at elevated temperatures. Upon cooling, the ordered states of cation occupancy are ‘frozen in’ and strains in lattice parameters are maintained, suggesting that re-equilibration during cooling has not taken place.


2011 ◽  
Vol 33 (2) ◽  
pp. 99 ◽  
Author(s):  
Joel Brown ◽  
Neil MacLeod

Rangeland ecosystems are capable of providing an array of ecosystem services important to the wellbeing of society. Some of these services (e.g. meat, fibre) are transported to markets and their quantity, quality and value are established via a set of widely accepted standards. Other services (e.g. climate mitigation, water quality, wildlife habitat) do not leave the land, but are, in fact, most valuable when they remain in situ. Determining their quantity, quality and value presents a challenge that must be met if there is to be a credible, accessible ecosystem services market for rangelands. In this paper we describe some of the ecosystem services that may be extracted from rangelands, discuss their unique ecological nature and relate those unique ecological properties to soil and vegetation attributes that can serve as a basis for measurement, both quality and quantity. We suggest the use of a soil/vegetation-based system in which similar climate, geomorphology and edaphic properties are grouped into ecological sites based on their response to disturbance. Within each ecological site, a unique state and transition model describes the dynamics of vegetation and soil surface properties, provides state indicators (vegetation structure, soil properties), predicts ecosystem services that may be derived at multiple scales, and organises information related to management to achieve ecosystem service objectives, including sustainability.


2020 ◽  
Vol 63 (3) ◽  
pp. pygs2020-003
Author(s):  
Stephen K. Donovan ◽  
Andrew Tenny

Salthill Quarry, Clitheroe, Lancashire (Mississippian, early Visean, Chadian) is a Site of Special Scientific Interest on the basis of its diverse fauna of invertebrates preserved in a Waulsortian mud mound setting. Important new specimens are described from the limestones and thin mudstones of the Hodder Mudstone Formation, on the southern margin of the quarry. A rare echinoid, archaeocidarid? gen. et sp. indet., is incomplete, but unusually retains the original curvature of the test. A columnal of Bystrowicrinus (col.) westheadi Donovan was derived from a radicular runner and has a dense cluster of radice scars on one side only. The columnal had a wedge-like section, thus suggesting a curvature of the stem. A cluster of solitary rugose corals on the base of a limestone bed includes at least two geniculated specimens in life position, the calice raised above the base of the bed. This is more likely to be due to burial in situ than a current accumulation. Ostiocavichnus Bohatý et al. is not an ichnotaxon, but a palaeopathology identified as a swollen crinoid pluricolumnal; broadly analogous structures are identified in fossil decapod crustaceans.


2013 ◽  
Vol 49 (2) ◽  
pp. 95-103 ◽  
Author(s):  
B. Chalmers ◽  
J. Elphick ◽  
G. Gilron ◽  
H. Bailey

This study evaluated an in situ early life stage test using cutthroat trout for potential use in Canada's Metal Mines Effluent Regulations' Environmental Effects Monitoring (EEM) program. Current field monitoring approaches focus on either adult fish surveys or mesocosm studies, but both of these have inherent limitations that may affect their suitability on a site-specific basis. This study evaluated an alternative approach, namely an in situ toxicity test, as part of an EEM program for a zinc, copper and gold mine. Hatchboxes containing cutthroat trout embryos were placed in a creek that receives treated effluent from the mine, and monitored through the swim-up stage to evaluate hatching success, survival, normal development and growth. Advantages of the method include: no feeding requirement during exposure, fixed exposure locations, relevant endpoints and high statistical sensitivity. In addition, the extended exposure period integrated long-term exposure variables, including low-flow and freshet events. This approach also has application to other salmonid species and types of discharges.


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