Faculty Opinions recommendation of Quantitative analysis of cytoskeletal reorganization during epithelial tissue sealing by large-volume electron tomography.

2015 ◽  
Author(s):  
Ronen Zaidel-Bar
Keyword(s):  
Quantitative Analysis ◽  
Large Volume ◽  
Electron Tomography ◽  
Epithelial Tissue ◽  
Cytoskeletal Reorganization ◽  
Tissue Sealing

Quantitative analysis of cytoskeletal reorganization during epithelial tissue sealing by large-volume electron tomography

2015 ◽  
Vol 17 (5) ◽  
pp. 605-614 ◽  
Author(s):  
Mikhail Eltsov ◽  
Nadia Dubé ◽  
Zhou Yu ◽  
Laurynas Pasakarnis ◽  
Uta Haselmann-Weiss ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Large Volume ◽  
Electron Tomography ◽  
Epithelial Tissue ◽  
Cytoskeletal Reorganization ◽  
Tissue Sealing

scholarly journals Cryo-electron Tomography Workflows for Quantitative Analysis of Actin Networks Involved in Cell Migration

2020 ◽  
Vol 26 (S2) ◽  
pp. 2518-2519
Author(s):  
Florian Fäßler ◽  
Georgi Dimchev ◽  
Victor-Valentin Hodirnau ◽  
Bettina Zens ◽  
Christoph Möhl ◽  
...  
Keyword(s):  
Cell Migration ◽  
Quantitative Analysis ◽  
Electron Tomography ◽  
Actin Networks ◽  
Cryo Electron Tomography

scholarly journals Effects of Different Divalent Cation Hydrothermal Treatments of Titanium Implant Surfaces for Epithelial Tissue Sealing

Materials ◽  
2020 ◽  
Vol 13 (9) ◽  
pp. 2038 ◽  
Author(s):  
Xudiyang Zhou ◽  
Ikiru Atsuta ◽  
Yasunori Ayukawa ◽  
Ikue Narimatsu ◽  
Tianren Zhou ◽  
...  
Keyword(s):  
Divalent Cations ◽  
Titanium Implant ◽  
Epithelial Tissue ◽  
Strontium Chloride ◽  
Implant Stability ◽  
Oral Epithelial ◽  
Laminin 332 ◽  
Positive Effect ◽  
Tissue Sealing

The improvement of peri-implant epithelium (PIE) adhesion to titanium (Ti) may promote Ti dental implant stability. This study aims to investigate whether there is a positive effect of Ti hydrothermally treated (HT) with calcium chloride (CaCl2), zinc chloride (ZnCl2), and strontium chloride (SrCl2) on promoting PIE sealing. We analyzed the response of a rat oral epithelial cell (OEC) culture and performed an in vivo study in which the maxillary right first molars of rats were extracted and replaced with calcium (Ca)-HT, zinc (Zn)-HT, strontium (Sr)-HT, or non-treated control (Cont) implants. The OEC adhesion on Ca-HT and Zn-HT Ti plates had a higher expression of adhesion proteins than cells on the Cont and Sr-HT Ti plates. Additionally, the implant PIE of the Ca-HT and Zn-HT groups revealed better expression of immunoreactive laminin-332 (Ln-322) at 2 weeks after implantation. The Ca-HT and Zn-HT groups also showed better attachment at the implant–PIE interface, which inhibited horseradish peroxidase penetration. These results demonstrated that the divalent cations of Ca (Ca2+) and Zn (Zn2+)-HT improve the integration of epithelium around the implant, which may facilitate the creation of a soft barrier around the implant to protect it from foreign body penetration.

scholarly journals El turismo procedente de Argentina en Uruguay: un análisis comportamental cuantitativo

2020 ◽  
pp. 239-264
Author(s):  
Eva Martín Roda ◽  
Silvana Sassano Luiz
Keyword(s):  
Quantitative Analysis ◽  
Economic Impact ◽  
Large Volume ◽  
Geographic Origin ◽  
National Statistics ◽  
El Alto ◽  
Strong Seasonality

El alto volumen de turistas que llegan a Uruguay procedentes desde Argentina, genera un alto impacto espacial, social y económico en ese país, caracterizado por una fuerte estacionalidad. El presente artículo realiza un análisis comportamental cuantitativo fundamentado en los datos procedentes de las encuestas aleatorias realizadas por el Instituto Nacional de Estadística de Uruguay a los turistas internacionales a su entrada en el país, analizándose factores como: origen geográfico, transporte utilizado, destino principal, lugar de entrada, características sociológicas entre otras variables. The large volume of tourists arriving in Uruguay, coming from Argentina, generate a high spatial, social and economic impact in that country. This impact has a strong seasonality. This article makes a quantitative analysis of the behavior of these flows. The analysis uses data from random surveys made to international tourists upon entering the country, by the National Statistics Institute of Uruguay. The surveys examine factors such as geographic origin, transportation used to enter, the main destination in this country, the place of entry to Uruguay and some sociological characteristics, among another variables.

scholarly journals Observation of Deformed GP Zone on the Shear Bands and Quantitative Analysis of Relative Strain by Three-dimensional Electron Tomography

Materia Japan ◽  
2007 ◽  
Vol 46 (12) ◽  
pp. 788-788
Author(s):  
Koji Inoke ◽  
Kenji Kaneko ◽  
Kenji Higashida ◽  
Zenji Horita ◽  
M. Weyland ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Electron Tomography ◽  
Shear Bands ◽  
Three Dimensional ◽  
Dimensional Electron ◽  
Relative Strain

scholarly journals Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Miriam S Weber ◽  
Matthias Eibauer ◽  
Suganya Sivagurunathan ◽  
Thomas M Magin ◽  
Robert D Goldman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Electron Tomography ◽  
Structural Heterogeneity ◽  
Epithelial Tissue ◽  
Cell Periphery ◽  
Cryo Electron Microscopy ◽  
Dimensional Classification ◽  
Keratin Filaments ◽  
Electron Dense Core ◽  
Subunit Organization

Keratin intermediate filaments are an essential and major component of the cytoskeleton in epithelial cells. They form a stable yet dynamic filamentous network extending from the nucleus to the cell periphery, which provides resistance to mechanical stresses. Mutations in keratin genes are related to a variety of epithelial tissue diseases. Despite their importance, the molecular structure of keratin filaments remains largely unknown. In this study, we analyzed the structure of keratin 5/keratin 14 filaments within ghost mouse keratinocytes by cryo-electron microscopy and cryo-electron tomography. By averaging a large number of keratin segments, we have gained insights into the helical architecture of the filaments. Two-dimensional classification revealed profound variations in the diameter of keratin filaments and their subunit organization. Computational reconstitution of filaments of substantial length uncovered a high degree of internal heterogeneity along single filaments, which can contain regions of helical symmetry, regions with less symmetry and regions with significant diameter fluctuations. Cross section views of filaments revealed that keratins form hollow cylinders consisting of multiple protofilaments, with an electron dense core located in the center of the filament. These findings shed light on the complex and remarkable heterogenic architecture of keratin filaments, suggesting that they are highly flexible, dynamic cytoskeletal structures.

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