scholarly journals Faculty Opinions recommendation of Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.

2021 ◽  
Author(s):  
Felipe Cava
Keyword(s):  
Cell Wall ◽  
Molecular Imaging ◽  
Cell Morphology ◽  
Bacterial Cell ◽  
Cell Wall Polysaccharide ◽  
Bacterial Cell Morphology

scholarly journals Two Homologous Agr-Like Quorum-Sensing Systems Cooperatively Control Adherence, Cell Morphology, and Cell Viability Properties in Lactobacillus plantarum WCFS1

2008 ◽  
Vol 190 (23) ◽  
pp. 7655-7665 ◽  
Author(s):  
Toshio Fujii ◽  
Colin Ingham ◽  
Jiro Nakayama ◽  
Marke Beerthuyzen ◽  
Ryoko Kunuki ◽  
...  
Keyword(s):  
Cell Wall ◽  
Quorum Sensing ◽  
Lactobacillus Plantarum ◽  
Cell Morphology ◽  
Response Regulator ◽  
Regulatory System ◽  
Mass Spectrometry Analysis ◽  
Cell Wall Polysaccharide ◽  
Transcription Analysis ◽  
Spectrometry Analysis

ABSTRACT A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for ΔlamA, ΔlamR, ΔlamA ΔlamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the ΔlamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the ΔlamA ΔlamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the ΔlamA and ΔlamA ΔlamR mutants. However, the regulation ratio was more significant for the ΔlamA ΔlamR mutant, indicating the cooperative effect of LamA and LamR.

Antibacterial activity of honeys from Amazonian stingless bees of Melipona spp. and its effects on bacterial cell morphology

2020 ◽  
Author(s):  
Susan Christina Braga Domingos ◽  
Victor Hugo Clebis ◽  
Gerson Nakazato ◽  
Admilton Gonçalves Oliveira ◽  
Renata Katsuko Takayama Kobayashi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cell Morphology ◽  
Bacterial Cell ◽  
Stingless Bees ◽  
Bacterial Cell Morphology

scholarly journals Leucine-Based Polymer Architecture-Induced Antimicrobial Properties and Bacterial Cell Morphology Switching

ACS Omega ◽  
2018 ◽  
Vol 3 (1) ◽  
pp. 769-780 ◽  
Author(s):  
Ishita Mukherjee ◽  
Anwesha Ghosh ◽  
Punyasloke Bhadury ◽  
Priyadarsi De
Keyword(s):  
Cell Morphology ◽  
Bacterial Cell ◽  
Antimicrobial Properties ◽  
Polymer Architecture ◽  
Bacterial Cell Morphology

scholarly journals Pharmacodynamics of linezolid-plus-fosfomycin against vancomycin-susceptible and -resistant enterococci in vitro and in vivo of a Galleria mellonella larval infection model

2019 ◽  
Author(s):  
Caifen Qi ◽  
Shuangli Xu ◽  
Maomao Wu ◽  
Shuo Zhu ◽  
Yanyan Liu ◽  
...  
Keyword(s):  
Combination Treatment ◽  
Cell Morphology ◽  
Bacterial Cell ◽  
Galleria Mellonella ◽  
Infection Model ◽  
Bacterial Killing ◽  
Bacterial Cell Morphology ◽  
Time Kill

AbstractObjectiveTo explore the in vitro and in vivo antibacterial activity of linezolid/fosfomycin combination against vancomycin-susceptible and -resistant enterococci (VSE and VRE), providing theoretical basis for the treatment of VRE.MethodsThe checkerboard method and time-kill curve study were used to evaluate the synergistic effect of linezolid combined with fosfomycin against VSE and VRE. The transmission electron microscopy (TEM) was employed to observe the bacterial cell morphology followed by each drug alone and in combination, elucidating the possible result of antibiotic combination therapy. The Galleria mellonella infection model was constructed to demonstrate the in vivo efficacy of linezolid plus fosfomycin for VSE and VRE infection.ResultsThe fractional inhibitory concentration index (FICI) values of all strains suggested that linezolid showed synergy or additivity in combination with fosfomycin against five of the six strains. Time-kill experiments demonstrated that the combination of linezolid-fosfomycin at 1×MIC or 2×MIC led to higher degree of bacterial killing without regrowth for all isolates tested than each monotherapy. TEM imaging showed that the combination treatment damaged the bacterial cell morphology more obviously than each drug alone. In the Galleria mellonella infection model, the enhanced survival rate of the combination treatment was revealed compared to linezolid monotherapy (P<0.05).ConclusionsOur data manifest that the combination of linezolid and fosfomycin may be a possible therapeutic regimen for VRE infection. The combination displays excellent bacterial killing and inhibits amplification of fosfomycin-resistant subpopulations.

scholarly journals Uji In Vitro Asap Cair terhadap Ralstonia syzygii subsp. celebesensis Penyebab Penyakit Darah pada Pisang

2018 ◽  
Vol 14 (4) ◽  
pp. 145
Author(s):  
Aisyah Imas ◽  
Giyanto Giyanto ◽  
Meity Suradji Sinaga ◽  
Abdjad Asih Nawangsih ◽  
Gustan Pari
Keyword(s):  
Cell Morphology ◽  
Bacterial Cell ◽  
Control Method ◽  
Diffusion Method ◽  
In Vitro Test ◽  
Tetrazolium Chloride ◽  
Liquid Smoke ◽  
Blood Disease ◽  
Bacterial Cell Morphology

In Vitro Test of Liquid Smoke against Ralstonia syzygii subsp. Celebesensis, the Cause of Blood Disease in Bananas Blood disease, caused by Ralstonia syzygii subsp. celebesensis, is an important disease of banana plants in Indonesia. Several control methods have been done, but the result were not effective.  Liquid smoke from wood waste is reported to be antimicrobial, but its use for controlling R. syzygii subsp. celebesensis has never been reported. Research was conducted to examine the ability of liquid smoke produced from coconut shell (CS-LS), pinecone (P-LS), and oil palm branch (OPB-LS) in inhibiting the growth of R. syzygii subsp. celebensensis in vitro and its effect on bacterial cell morphology. Efficacy test of liquid smoke was carried out by agar diffusion method and measurement of bacterial density by spectrophotometry with ʎ 600 nm. Observation of bacterial cell morphology was carried out by electron microscopy. The CS-LS, P-LS, and OPB-LS starting from 0.5% concentration showed inhibitory activity against R. syzygii subsp. celebesensis both on triphenyl tetrazolium chloride medium and luria bertani broth. Liquid smoke also caused damage to cell walls and cell membranes. Therefore, liquid smoke has the potential to be used as component in control method for R. syzygii subsp. celebesensis.

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