scholarly journals Characterisation of a thermotolerant yeast, Kluyveromyces marxianus CBS712

2009 ◽  
pp. 7-13
Author(s):  
Éva Erdei ◽  
Mónika Molnár ◽  
Gyöngyi Gyémánt ◽  
János Harangi ◽  
János Nagy ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Glucose Concentration ◽  
Temperature Limit ◽  
Thermotolerant Yeast ◽  
Bioethanol Production ◽  
Technological Advantage ◽  
Different Temperatures ◽  
Higher Temperature

Fermentation at high temperature with application of thermotolerant microorganisms is a technological advantage in bioethanol production. Among the yeasts, K. marxianus has outstanding thermotolarance. The industrial application of the IMB3 strain occurs usually at 45C. The final aim of our project is the genetic modification of the K. marxianus CBS712 strain in order to achieve ethanol production at higher temperature than the currently applied. This requires the characterization of the CBS712 strain, with special attention to the determination of the temperature limit of its growth and the amount of the ethanol produced. The temperature limit of growth was 48C in YPD medium. Elevation of the temperature above 45C led to an exponential drop of the cell viability. Ethanol production was tested in shaking flasks, in MYFM medium, under oxigene limited conditions, applying variable concentrations of glucose (12–20%) and different temperatures (45–47 ºC). Preliminary results have revealed that the elevation of glucose concentration increased the amount of ethanol produced. The amount of ethanol (appr. 5%)+ produced at the highest glucose concentration was not different at the tested temperatures (45, 46 and 47 ºC). The observation indicates the potential in raising the thermotolerance of the strain. 

scholarly journals Physical characterization of Rhipsalis (Cactaceae) fruits and seeds germination in different temperatures and light regimes

2016 ◽  
Vol 76 (2) ◽  
pp. 367-373 ◽  
Author(s):  
A. B. Lone ◽  
R. C. Colombo ◽  
B. L. G. Andrade ◽  
L. S. A. Takahashi ◽  
R. T. Faria
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Light Regimes ◽  
Total Absence ◽  
Germination Characteristics ◽  
Different Temperatures ◽  
Medium Time ◽  
Seeds Germination

Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.

Microstructural Characterization and Evaluation of Mechanical Properties of Steel with Different Biphasic Microstructures Obtained from LNE 500 Steel

2014 ◽  
Vol 805 ◽  
pp. 227-230
Author(s):  
Alexandre Nogueira Ottoboni Dias ◽  
Márcia Regina Baldissera ◽  
Eduardo Carvalho Rossi ◽  
Gilberto Carvalho Coelho ◽  
Andersan dos Santos Paula ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Stress Test ◽  
Microstructural Characterization ◽  
Uniaxial Stress ◽  
Total Elongation ◽  
Different Temperatures ◽  
Intercritical Region ◽  
Thermocalc Software

In this work was carried out the microstructural characterization and evaluation of mechanical properties of steel with different microstructures. The intercritical region and the existing phases in function of temperature were determined using the THERMOCALC software. The samples of steel were quenched at different temperatures to obtain differents microstructures consisting of ferrite, pearlite and martensite. The microstructural characterization of the samples was performed by qualitative and quantitative metallography. The determination of volume was performed with the "Image J" software. The mechanical properties were determined by uniaxial stress test, which determined the parameters: yield strength, tensile strength, breaking point and total elongation.

scholarly journals Biochemical and kinetic characterization of laccase and manganese peroxidase from novelKlebsiella pneumoniaestrains and their application in bioethanol production

RSC Advances ◽  
2018 ◽  
Vol 8 (27) ◽  
pp. 15044-15055 ◽  
Author(s):  
Nisha Gaur ◽  
Korrapati Narasimhulu ◽  
Pydisetty Y
Keyword(s):  
Ethanol Production ◽  
Wheat Bran ◽  
Manganese Peroxidase ◽  
Kinetic Characterization ◽  
Bioethanol Production

Upto 3 fold purified laccase and MnP from novelKlebsiellaisolates, mediated ethanol production from rice and wheat bran substrates lead to almost 40% improvement in production profile.

scholarly journals Physiological characterization of thermotolerant yeast for cellulosic ethanol production

2014 ◽  
Vol 98 (8) ◽  
pp. 3829-3840 ◽  
Author(s):  
Daniela A. Costa ◽  
Carlos J. A. de Souza ◽  
Patrícia S. Costa ◽  
Marina Q. R. B. Rodrigues ◽  
Ancély F. dos Santos ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Thermotolerant Yeast ◽  
Physiological Characterization

Preparation and Characterization of CaSiO3-Ca3(PO4)2 Composite Bioceramics

2007 ◽  
Vol 330-332 ◽  
pp. 451-454
Author(s):  
Si Yu Ni ◽  
Jiang Chang ◽  
Kai Li Lin ◽  
Wan Yin Zhai
Keyword(s):  
Body Fluid ◽  
Degradation Rate ◽  
X Ray Diffraction ◽  
Buffer Solution ◽  
Composite Surface ◽  
X Ray ◽  
Different Temperatures ◽  
Higher Temperature

In this study, CaSiO3 (CS)/Ca3(PO4)2 (TCP) composites with 50% CS and 50% TCP sintered at different temperatures (1100oC, 1200oC and 1300oC) were prepared. The formation of bone-like apatite on CS-TCP composites was investigated by soaking the ceramics in simulated body fluid (SBF), and the presence of bone-like apatite layer on the composite surface after soaking in SBF was determined by X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). The results showed that the bone-like apatite was formed on all the CS-TCP composites sintered at different temperatures after 7 days of immersion. In addition, the degradation of CS-TCP composites prepared at different temperatures was evaluated by measurement of weight loss of the ceramics in Tris-HCl buffer solution at 37oC, and the results showed that there was no difference in degradation rate between the samples. In vitro cell experiments indicated that the osteoblasts proliferated faster on the CS-TCP ceramics sintered at higher temperature, and cells on the CS-TCP ceramics sintered at 1300oC showed highest proliferation rate. These results provide valuable information for designing CS-TCP composite bioceramics for bone regeneration applications.

scholarly journals Evaluation of various silo sorghum hybrids from the energetic aspect

2013 ◽  
pp. 107-111
Author(s):  
János Jóvér ◽  
Éva Ábrahám ◽  
Lajos Blaskó
Keyword(s):  
Ethanol Production ◽  
Sweet Sorghum ◽  
Sugar Content ◽  
Production Capacity ◽  
Bioethanol Production ◽  
Heating Value ◽  
Production Site ◽  
Sorghum Bagasse ◽  
Energetic Aspect

The sweet sorghum is a perspective plant of bioenergy, which can be the foundation of Hungarian bioethanol production in the future. By the examination six sweet sorghum hybrids have been examinated by the viewpoint of sugar aggregation and bioethanol production capacity. The founda tion of the surve y was three growing se ason (201 0, 2011, 2012 ). The ex per ime nt w as on the production sites of U niversity of Debrecen CAAES RISF Karcag Research Institute. The production site of the sweet sorghum have been sampled by samples of 1 m2 by hybrids within the period of august 15 and november 15. The sugar content of the samples have been measured by refractometer which was the base in the determination of ethanol production capacity. As a continuation of the evaluation of energetic viewpoint the Higher Heating Value (HHV) have been measured from the bagasse of sweet sorghums. According to the results it can be stated that int he case of sugarcontent and the dinamics of the sugar aggregation can show several differences, while HHV of sorghum bagasse is within 16 200 and 16 900 J g-1.

NMR Characterization of Silicon Carbides and Carbonitrides. A Method for Quantifying the Silicon Sites and the Free Carbon Phase.

1992 ◽  
Vol 271 ◽  
Author(s):  
C. Gerardin ◽  
M. Henry ◽  
F. Taulelle
Keyword(s):  
A Priori ◽  
Free Carbon ◽  
Carbon Phase ◽  
Nmr Characterization ◽  
Different Temperatures ◽  
Organic Precursors ◽  
Nmr Methods ◽  
Shift Calculation

ABSTRACTWith ceramics prepared from organic precursors, polymers are often formed by thermolysis, and the ceramics are obtained by pyrolysis of the polymer. This paper will cover the NMR methods used to characterize the polymerization as well as the pyrolysis of such polymers. The reticulation rate of such materials is measured on the silicon atoms and on the carbon atoms, leading to a silicon reticulation state and a carbon reticulation state. For the ceramics, the quantification of the SiCxH4−x or SiCxN4−x sites is performed using a priori chemical shift calculation. A measure of the H/C of the free carbon phase at the different temperatures of pyrolysis is presented. This measure uses a absolute determination of hydrogen content in ceramics, more accurate than a classical chemical analysis of hydrogen. The evolution of the reticulation, of the free carbon amount and of the H/C ratio in the free carbon phase will be followed with the temperature.

Quantitative Assessment of Soluble Fibrin in Plasma by Affinity Chromatography – A Comparative Study with desAA-Fibrin, desAABB-Fibrin and Fibrinogen

1985 ◽  
Vol 54 (02) ◽  
pp. 533-538 ◽  
Author(s):  
Wilfried Thiel ◽  
Ulrich Delvos ◽  
Gert Müller-Berghaus
Keyword(s):  
Affinity Chromatography ◽  
Calcium Ions ◽  
Quantitative Assessment ◽  
Fluid Phase ◽  
Soluble Fibrin ◽  
Binding Characteristics ◽  
Different Temperatures ◽  
Immobilized Proteins ◽  
Sepharose 4B

SummaryA quantitative determination of soluble fibrin in plasma was carried out by affinity chromatography. For this purpose, desAA-fibrin and fibrinogen immobilized on Sepharose 4B were used at the stationary side whereas batroxobin-induced 125I-desAA-fibrin or thrombin-induced 125I-desAABB-fibrin mixed with plasma containing 131I-fibrinogen represented the fluid phase. The binding characteristics of these mixtures to the immobilized proteins were compared at 20° C and 37° C. Complete binding of both types of fibrin to the immobilized desAA-fibrin was always seen at 20° C as well as at 37° C. However, binding of soluble fibrin was accompanied by substantial binding of fibrinogen that was more pronounced at 20° C. Striking differences depending on the temperature at which the affinity chromatography was carried out, were documented for the fibrinogen-fibrin interaction. At 20° C more than 90% of the applied desAA-fibrin was bound to the immobilized fibrinogen whereas at 37° C only a mean of 17% were retained at the fibrinogen-Sepharose column. An opposite finding with regard to the tested temperature was made with the desAABB-fibrin. Nearly complete binding to insolubilized fibrinogen was found at 37° C (95%) but only 58% of the desAABB-fibrin were bound at 20° C. The binding patterns did not change when the experiments were performed in the presence of calcium ions. The opposite behaviour of the two types of soluble fibrin to immobilized fibrinogen at the different temperatures, together with the substantial binding of fibrinogen in the presence of soluble fibrin to insolubilized fibrin in every setting tested, devaluates affinity chromatography as a tool in the quantitative assessment of soluble fibrin in patients’ plasma.

Characterization of Impressed Current Technique to Model Corrosion of Reinforcement in Concrete

2020 ◽  
Vol 11 (1) ◽  
pp. 93-99
Author(s):  
Abu Zakir Morshed ◽  
Sheikh Shakib ◽  
Tanzim Jahin
Keyword(s):  
Immersion Time ◽  
Chloride Content ◽  
Engineering Science ◽  
Coastal Regions ◽  
Faraday’S Law ◽  
Current Technique ◽  
Corrosion Cell ◽  
Impressed Current

Corrosion of reinforcement is an important durability concern for the structures exposed to coastal regions. Since corrosion of reinforcement involves long periods of time, impressed current technique is usually used to accelerate the corrosion of reinforcement in laboratories. Characterization of impressed current technique was the main focus of this research,which involved determination of optimum chloride content and minimum immersion time of specimens for which the application of Faraday’s law could be efficient. To obtain optimum chloride content, the electrolytes in the corrosion cell were prepared similar to that of concrete pore solutions. Concrete prisms of 200 mm by 200 mm by 300 mm were used to determine the minimum immersion time for saturation. It was found that the optimum chloride content was 35 gm/L and the minimum immersion time for saturation was 140 hours. Accounting the results, a modified expression based on Faraday’s law was proposed to calculate weight loss due to corrosion. Journal of Engineering Science 11(1), 2020, 93-99

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