Análise toxicológica da cannabis sativa e seus benefícios terapêuticos / Toxicological analysis of cannabis sativa and its therapeutic benefits

There is a growing interest in the production of hemp for the extraction of cannabidiol (CBD) due to reported therapeutic benefits. Recent policy reform has permitted state hemp pilot programs, including the land grant research institutions, the ability to investigate the potential of growing and harvesting Cannabis sativa plants (≤0.3% tetrahydrocannabinol) for these purposes in the U.S. There are vast gaps of knowledge regarding the fertility requirements of hemp cultivars grown in a horticultural production setting for floral attributes such as the cannabinoid constituents. Foliar tissue analysis provides an avenue to determine adequate ranges for nutrient uptake and estimating fertilizer requirements prior to visual symptoms of deficiency or toxicity. To facilitate a survey range of elemental nutrient acquisition in hemp cultivars propagated for CBD production, foliar analysis was executed using the most recently mature leaves (MRML) of mother stock plants. All plants were maintained in the vegetative stage for twelve weeks, prior to initiation of cutting for clone harvesting. A total of thirteen cultivars were utilized to broaden previously reported baseline survey ranges. Significant differences were found among all thirteen cultivars in accumulation of both micro and macro essential nutrients, widening the range of the fertility requirements of Cannabis plants grown in this production model for CBD harvesting.

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A Novel Standardized Cannabis sativa L. Extract and Its Constituent Cannabidiol Inhibit Human Polymorphonuclear Leukocyte Functions

International Journal of Molecular Sciences ◽

10.3390/ijms20081833 ◽

2019 ◽

Vol 20 (8) ◽

pp. 1833 ◽

Cited By ~ 4

Author(s):

Alex Mabou Tagne ◽

Franca Marino ◽

Massimiliano Legnaro ◽

Alessandra Luini ◽

Barbara Pacchetti ◽

...

Keyword(s):

Cell Migration ◽

Oxidative Metabolism ◽

Molecular Mechanisms ◽

Cannabis Sativa ◽

Biological Effects ◽

Enzyme Linked Immunosorbent Assay ◽

Boyden Chamber ◽

Ros Production ◽

Therapeutic Benefits ◽

Tnf Α

Cannabis and cannabinoids offer significant therapeutic benefits for a wide scope of pathological conditions. Among them, the clinical issues rooted in inflammation stand out, nonetheless, the underlying mechanisms are not yet plainly understood. Circumstantial evidence points to polymorphonuclear leukocytes (PMN) as targets for the anti-inflammatory effects of cannabis. Therefore, we conducted this study to assess the effects of CM5, a novel Cannabis sativa L. extract standardized in 5% cannabidiol (CBD), on human PMN functions, including cell migration, oxidative metabolism and production of tumour necrosis factor (TNF)-α. We then sought to investigate whether such effects could be ascribed to its content in CBD. Cell migration was assessed by the Boyden chamber assay, oxidative metabolism by means of spectrofluorimetric measurement of reactive oxygen species (ROS) production, and TNF-α was measured by real time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Results show that both CM5 and CBD inhibit PMN migration, ROS and TNF-α production, indicating that CBD may be the main item responsible for the effects of CM5. CM5 is however more potent than CBD on cell migration and TNF-α production, and less effective on ROS production, suggesting that beyond CBD, other components of the cannabis plant may contribute to the biological effects of the extract. As a whole, such results support the use of cannabis standardized extract and CBD to stem inflammation; however, they also warrant in-depth investigation of the underlying cellular and molecular mechanisms to better exploit their therapeutic potential.

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A Proteomic View of Cellular and Molecular Effects of Cannabis

Biomolecules ◽

10.3390/biom11101411 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1411

Author(s):

Morteza Abyadeh ◽

Vivek Gupta ◽

Joao A. Paulo ◽

Veer Gupta ◽

Nitin Chitranshi ◽

...

Keyword(s):

Cannabis Sativa ◽

Cellular Level ◽

Chemical Components ◽

Active Components ◽

Comprehensive Overview ◽

Therapeutic Benefits ◽

A Cell ◽

Molecular Effects

Cannabis (Cannabis sativa), popularly known as marijuana, is the most commonly used psychoactive substance and is considered illicit in most countries worldwide. However, a growing body of research has provided evidence of the therapeutic properties of chemical components of cannabis known as cannabinoids against several diseases including Alzheimer’s disease (AD), multiple sclerosis (MS), Parkinson’s disease, schizophrenia and glaucoma; these have prompted changes in medicinal cannabis legislation. The relaxation of legal restrictions and increased socio-cultural acceptance has led to its increase in both medicinal and recreational usage. Several biochemically active components of cannabis have a range of effects on the biological system. There is an urgent need for more research to better understand the molecular and biochemical effects of cannabis at a cellular level, to understand fully its implications as a pharmaceutical drug. Proteomics technology is an efficient tool to rigorously elucidate the mechanistic effects of cannabis on the human body in a cell and tissue-specific manner, drawing conclusions associated with its toxicity as well as therapeutic benefits, safety and efficacy profiles. This review provides a comprehensive overview of both in vitro and in vivo proteomic studies involving the cellular and molecular effects of cannabis and cannabis-derived compounds.

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Cannabis und Schizophrenie

SUCHT - Zeitschrift für Wissenschaft und Praxis / Journal of Addiction Research and Practice ◽

10.1024/suc.2002.48.5.336 ◽

2002 ◽

Vol 48 (5) ◽

pp. 336-345 ◽

Cited By ~ 3

Author(s):

Jürgen Seifert ◽

Jann Schlimme ◽

Felix Wedegärtner ◽

Hinderk M. Emrich ◽

Udo Schneider

Keyword(s):

Cannabis Sativa ◽

Cannabinoid System ◽

Psychotische Störungen

Überblick: Cannabis sativa ist eine der ältesten medizinischen Heilpflanzen. In den westlichen Kulturen wird Cannabis überwiegend als Freizeitdroge angesehen. Im Harz sowie in den Blüten der weiblichen Pflanze findet sich ein Gemisch aus verschiedenen psychoaktiven Inhaltsstoffen. Am bedeutendsten ist in diesem Zusammenhang das delta-9-Tetrahy-drocannabinol. </P><P> Epidemiologie: Nicht selten werden die Gefahren des Cannabiskonsums, wie z. B. die Möglichkeit der Induktion einer Psychose unterschätzt. Wenn man jedoch berücksichtigt, wie häufig z. B. Jugendliche THC konsumieren, ist die Frage, ob Cannabis eine Psychose aus dem schizophrenen Formenkreis de novo induzieren kann, von besonderem gesundheitspolitischem und volkswirtschaftlichem Interesse. Die Datenlage ist allerdings nach wie vor unklar. Die klinisch-epidemiologischen Studien deuten aber darauf hin, dass Cannabiskonsum bei vulnerablen Personen schizophrenieforme Psychosen auslösen kann und einen ungünstigen Krankheitsverlauf zur Folge hat. </P><P> Das endogene Cannabinoid-System: Die vorliegende Arbeit gibt außerdem einen Überblick der Publikationen, die sich mit dem körpereigenen Cannabinoid- System und seinen endogenen Liganden im Hinblick auf psychotische Störungen beschäftigen.

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