scholarly journals Augmenting Nutrient Acquisition Ranges of Greenhouse Grown CBD (Cannabidiol) Hemp (Cannabis sativa) Cultivars

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 98
Author(s):  
Jennifer Kalinowski ◽  
Keith Edmisten ◽  
Jeanine Davis ◽  
Michelle McGinnis ◽  
Kristin Hicks ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Nutrient Acquisition ◽  
Production Model ◽  
Baseline Survey ◽  
Tissue Analysis ◽  
Mature Leaves ◽  
Therapeutic Benefits ◽  
Pilot Programs ◽  
Foliar Tissue ◽  
Recent Policy

There is a growing interest in the production of hemp for the extraction of cannabidiol (CBD) due to reported therapeutic benefits. Recent policy reform has permitted state hemp pilot programs, including the land grant research institutions, the ability to investigate the potential of growing and harvesting Cannabis sativa plants (≤0.3% tetrahydrocannabinol) for these purposes in the U.S. There are vast gaps of knowledge regarding the fertility requirements of hemp cultivars grown in a horticultural production setting for floral attributes such as the cannabinoid constituents. Foliar tissue analysis provides an avenue to determine adequate ranges for nutrient uptake and estimating fertilizer requirements prior to visual symptoms of deficiency or toxicity. To facilitate a survey range of elemental nutrient acquisition in hemp cultivars propagated for CBD production, foliar analysis was executed using the most recently mature leaves (MRML) of mother stock plants. All plants were maintained in the vegetative stage for twelve weeks, prior to initiation of cutting for clone harvesting. A total of thirteen cultivars were utilized to broaden previously reported baseline survey ranges. Significant differences were found among all thirteen cultivars in accumulation of both micro and macro essential nutrients, widening the range of the fertility requirements of Cannabis plants grown in this production model for CBD harvesting.

scholarly journals Characterization of Nutrient Disorders of Cannabis sativa

2019 ◽  
Vol 9 (20) ◽  
pp. 4432 ◽  
Author(s):  
Paul Cockson ◽  
Hunter Landis ◽  
Turner Smith ◽  
Kristin Hicks ◽  
Brian E. Whipker
Keyword(s):  
Plant Tissue ◽  
Cannabis Sativa ◽  
Nitrate Solution ◽  
Nutrient Deficiency ◽  
Essential Elements ◽  
Silica Sand ◽  
Sand Culture ◽  
Nutrient Concentrations ◽  
Tissue Analysis ◽  
Tissue Concentrations

Essential plant nutrients are needed at crop-specific concentrations to obtain optimum growth or yield. Plant tissue (foliar) analysis is the standard method for measuring those levels in crops. Symptoms of nutrient deficiency occur when those tissue concentrations fall to a level where growth or yield is negatively impacted and can serve as a visual diagnostic tool for growers and researchers. Both nutrient deficiency symptoms and their corresponding plant tissue concentrations have not been established for cannabis. To establish nutrient concentrations when deficiency or toxicity symptoms are expressed, Cannabis sativa ‘T1’ plants were grown in silica sand culture, and control plants received a complete modified Hoagland’s all-nitrate solution, whereas nutrient-deficient treatments were induced with a complete nutrient formula withholding a single nutrient. Toxicity treatments were induced by increasing the element tenfold higher than the complete nutrient formula. Plants were monitored daily and, once symptoms manifested, plant tissue analysis of all essential elements was performed by most recent mature leaf (MRML) tissue analysis, and descriptions and photographs of nutrient disorder symptomology were taken. Symptoms and progressions were tracked through initial, intermediate, and advanced stages. Information in this study can be used to diagnose nutrient disorders in Cannabis sativa.

scholarly journals A Novel Standardized Cannabis sativa L. Extract and Its Constituent Cannabidiol Inhibit Human Polymorphonuclear Leukocyte Functions

2019 ◽  
Vol 20 (8) ◽  
pp. 1833 ◽  
Author(s):  
Alex Mabou Tagne ◽  
Franca Marino ◽  
Massimiliano Legnaro ◽  
Alessandra Luini ◽  
Barbara Pacchetti ◽  
...  
Keyword(s):  
Cell Migration ◽  
Oxidative Metabolism ◽  
Molecular Mechanisms ◽  
Cannabis Sativa ◽  
Biological Effects ◽  
Enzyme Linked Immunosorbent Assay ◽  
Boyden Chamber ◽  
Ros Production ◽  
Therapeutic Benefits ◽  
Tnf Α

Cannabis and cannabinoids offer significant therapeutic benefits for a wide scope of pathological conditions. Among them, the clinical issues rooted in inflammation stand out, nonetheless, the underlying mechanisms are not yet plainly understood. Circumstantial evidence points to polymorphonuclear leukocytes (PMN) as targets for the anti-inflammatory effects of cannabis. Therefore, we conducted this study to assess the effects of CM5, a novel Cannabis sativa L. extract standardized in 5% cannabidiol (CBD), on human PMN functions, including cell migration, oxidative metabolism and production of tumour necrosis factor (TNF)-α. We then sought to investigate whether such effects could be ascribed to its content in CBD. Cell migration was assessed by the Boyden chamber assay, oxidative metabolism by means of spectrofluorimetric measurement of reactive oxygen species (ROS) production, and TNF-α was measured by real time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Results show that both CM5 and CBD inhibit PMN migration, ROS and TNF-α production, indicating that CBD may be the main item responsible for the effects of CM5. CM5 is however more potent than CBD on cell migration and TNF-α production, and less effective on ROS production, suggesting that beyond CBD, other components of the cannabis plant may contribute to the biological effects of the extract. As a whole, such results support the use of cannabis standardized extract and CBD to stem inflammation; however, they also warrant in-depth investigation of the underlying cellular and molecular mechanisms to better exploit their therapeutic potential.

scholarly journals A Proteomic View of Cellular and Molecular Effects of Cannabis

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1411
Author(s):  
Morteza Abyadeh ◽  
Vivek Gupta ◽  
Joao A. Paulo ◽  
Veer Gupta ◽  
Nitin Chitranshi ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Cellular Level ◽  
Chemical Components ◽  
Active Components ◽  
Comprehensive Overview ◽  
Therapeutic Benefits ◽  
A Cell ◽  
Molecular Effects

Cannabis (Cannabis sativa), popularly known as marijuana, is the most commonly used psychoactive substance and is considered illicit in most countries worldwide. However, a growing body of research has provided evidence of the therapeutic properties of chemical components of cannabis known as cannabinoids against several diseases including Alzheimer’s disease (AD), multiple sclerosis (MS), Parkinson’s disease, schizophrenia and glaucoma; these have prompted changes in medicinal cannabis legislation. The relaxation of legal restrictions and increased socio-cultural acceptance has led to its increase in both medicinal and recreational usage. Several biochemically active components of cannabis have a range of effects on the biological system. There is an urgent need for more research to better understand the molecular and biochemical effects of cannabis at a cellular level, to understand fully its implications as a pharmaceutical drug. Proteomics technology is an efficient tool to rigorously elucidate the mechanistic effects of cannabis on the human body in a cell and tissue-specific manner, drawing conclusions associated with its toxicity as well as therapeutic benefits, safety and efficacy profiles. This review provides a comprehensive overview of both in vitro and in vivo proteomic studies involving the cellular and molecular effects of cannabis and cannabis-derived compounds.

scholarly journals 645 Foliar Symptomology and Tissue Concentrations of Nutrient Deficient Ornamental Cabbage Plants

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 508E-509
Author(s):  
James L. Gibson ◽  
Brian E. Whipker ◽  
Dharmalingam S. Pitchay ◽  
Paul V. Nelson ◽  
C. Ray Campbell
Keyword(s):  
Tissue Concentration ◽  
Block Design ◽  
Dry Weight ◽  
Tissue Analysis ◽  
Concentration Data ◽  
Deficiency Symptoms ◽  
Mature Leaves ◽  
Foliar Symptoms ◽  
Young Leaves ◽  
Plastic Containers

Elemental deficiencies of N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, and B were induced in `Osaka White' ornamental cabbage (Brassica oleracea var. acephala L.) plants. Seedlings were planted in 4.7-L plastic containers and fertilized with a complete modified Hoagland's solution or this solution minus the element that was to be investigated. Plants were harvested for tissue analysis as well as dry weight when initial foliar symptoms were expressed and later under advanced deficiency symptoms. Root architecture was also recorded for the plants treated with the solutions. The containers were replicated three times for each of the two harvests and were randomized in a complete-block design. Deficiency symptoms for all treatments were observed within five weeks. The most dramatic expression of foliar symptoms occurred with N (a purplish tinge on underside of lower foliage leading to necrotic margins on the mature leaves), P (elongated internodes and a purplish tinge on underside of mature leaves), K (compact internodes with chlorotic lower foliage leading to necrotic patches on the leaf margins and blade), Fe (bright yellow upper foliage leading to a bleach white appearance), Ca (complete meristem necrosis with lower foliage becoming chlorotic then necrotic), and B (deformed young leaves and fully expanded leaves becoming thick, leathery, and brittle). The dry weight of plants treated with solutions not containing N, P, Ca, Fe, or B was significantly lower when compared to the control. Foliar tissue concentration data will assist plant tissue analysis laboratories in establishing foliar symptom standards for grower samples.

scholarly journals Seasonality in nutrient content of banana diagnostic leaf

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Larissa Grasiela de Arruda Ferreira Costa ◽  
Danilo Eduardo Rozane ◽  
Sarah de Araújo Silva ◽  
Cibelle Tamiris de Oliveira ◽  
Levi Godke Faber Pavarin ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Analysis Of Variance ◽  
Agricultural Production ◽  
Nutrient Content ◽  
Tissue Analysis ◽  
Banana Tree ◽  
Standard Sampling ◽  
Foliar Tissue ◽  
Important Position ◽  
The City

Abstract Banana farming has an important position in Brazilian agricultural production. This position was earned due to the use of cultivation technologies and adequate fertilization made through recommendations of evaluation of foliar analyzes. The objective of this study was to determine the seasonality of the nutritional status of the diagnostic leaf during the development of banana tree. The experiments took place in the city of Sete Barras / SP, with cultivars such as Grande naine and Prata, on summer and winter. It was evaluated the influence of the collection of the diagnostic leaf in the stations, in six seasons during the development of the banana bunch and it was compared with the standard sampling. The values were submitted to analysis of variance, and when the F was significant, a comparison of means was performed. The sampling stations and the collection stages influence the nutrient behavior in the diagnostic leaf of both varieties, with exceptions for Mg and Mn. The average levels of nutrients during the collection stages showed no difference among the 1st, 2nd, 3rd and 4th (standard). Even for the ‘Prata’ and Grande Naine banana plants cultivated in the Ribeira Valley, there is no significant alteration in the nutrient content of the foliage among the 1st to 4th stages, allowing an increasing of collection for foliar tissue analysis.

The association of ankle brachial index with left ventricular hypertrophy and left ventricular mass index: the Dong-gu study

VASA ◽  
2013 ◽  
Vol 42 (4) ◽  
pp. 284-291 ◽  
Author(s):  
Seong-Woo Choi ◽  
Hye-Yeon Kim ◽  
Hye-Ran Ahn ◽  
Young-Hoon Lee ◽  
Sun-Seog Kweon ◽  
...  
Keyword(s):  
Left Ventricular Hypertrophy ◽  
Left Ventricular Mass ◽  
Ventricular Hypertrophy ◽  
Left Ventricular Mass Index ◽  
Ankle Brachial Index ◽  
Intima Media Thickness ◽  
Left Ventricular ◽  
Baseline Survey ◽  
Ventricular Mass Index ◽  
Ventricular Mass

Background: To investigate the association between ankle-brachial index (ABI), left ventricular hypertrophy (LVH) and left ventricular mass index (LVMI) in a general population. Patients and methods: The study population consisted of 8,246 people aged 50 years and older who participated in the baseline survey of the Dong-gu Study conducted in Korea between 2007 and 2010. Trained research technicians measured LV mass using mode M ultrasound echocardiography and ABI using an oscillometric method. Results: After adjustment for risk factors and common carotid artery intima-media thickness (CCA-IMT) and the number of plaques, higher ABIs (1.10 1.19, 1.20 - 1.29, and ≥ 1.30) were significantly and linearly associated with high LVMI (1.10 - 1.19 ABI: β, 3.33; 95 % CI, 1.72 - 4.93; 1.20 - 1.29 ABI: β, 6.51; 95 % CI, 4.02 - 9.00; ≥ 1.30 ABI: β, 14.83; 95 % CI, 6.18 - 23.48). An ABI of 1.10 - 1.19 and 1.20 - 1.29 ABI was significantly associated with LVH (1.10 - 1.19 ABI: OR, 1.35; 95 % CI, 1.19 - 1.53; 1.20 - 1.29 ABI: OR, 1.59; 95 % CI, 1.31 - 1.92) and ABI ≥ 1.30 was marginally associated with LVH (OR, 1.73; 95 % CI, 0.93 - 3.22, p = 0.078). Conclusions: After adjustment for other cardiovascular variables and CCA-IMT and the number of plaques, higher ABIs are associated with LVH and LVMI in Koreans aged 50 years and older.

Cannabis und Schizophrenie

2002 ◽  
Vol 48 (5) ◽  
pp. 336-345 ◽  
Author(s):  
Jürgen Seifert ◽  
Jann Schlimme ◽  
Felix Wedegärtner ◽  
Hinderk M. Emrich ◽  
Udo Schneider
Keyword(s):  
Cannabis Sativa ◽  
Cannabinoid System ◽  
Psychotische Störungen

Überblick: Cannabis sativa ist eine der ältesten medizinischen Heilpflanzen. In den westlichen Kulturen wird Cannabis überwiegend als Freizeitdroge angesehen. Im Harz sowie in den Blüten der weiblichen Pflanze findet sich ein Gemisch aus verschiedenen psychoaktiven Inhaltsstoffen. Am bedeutendsten ist in diesem Zusammenhang das delta-9-Tetrahy-drocannabinol. </P><P> Epidemiologie: Nicht selten werden die Gefahren des Cannabiskonsums, wie z. B. die Möglichkeit der Induktion einer Psychose unterschätzt. Wenn man jedoch berücksichtigt, wie häufig z. B. Jugendliche THC konsumieren, ist die Frage, ob Cannabis eine Psychose aus dem schizophrenen Formenkreis de novo induzieren kann, von besonderem gesundheitspolitischem und volkswirtschaftlichem Interesse. Die Datenlage ist allerdings nach wie vor unklar. Die klinisch-epidemiologischen Studien deuten aber darauf hin, dass Cannabiskonsum bei vulnerablen Personen schizophrenieforme Psychosen auslösen kann und einen ungünstigen Krankheitsverlauf zur Folge hat. </P><P> Das endogene Cannabinoid-System: Die vorliegende Arbeit gibt außerdem einen Überblick der Publikationen, die sich mit dem körpereigenen Cannabinoid- System und seinen endogenen Liganden im Hinblick auf psychotische Störungen beschäftigen.

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