scholarly journals La importancia de la aplicación de micorrizas para disminuir la abscisión de naranja (Citrus sinensis L. Osbeck) en Veracruz, México / The importance of the application of mycorrhizae to reduce abscission of orange in Veracruz, Mexico

2021 ◽  
Vol 4 (4) ◽  
pp. 6012-6020
Author(s):  
Manuel Ángel Gómez Cruz ◽  
Laura Gómez Tovar ◽  
Luis Enrique Ortíz-Martínez ◽  
Alejandro Hernández-Carlos
Keyword(s):  
Citrus Sinensis ◽  
Citrus Tristeza Virus ◽  
Candidatus Liberibacter Asiaticus ◽  
Fusarium Spp ◽  
Stunt Viroid ◽  
Hop Stunt Viroid ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Colletotrichum Spp ◽  
Tristeza Virus

México es el cuarto productor de cítricos a nivel mundial (FAOSTAT, 2019) y el estado de Veracruz aporta el 53 % de la producción nacional (SIAP, 2019). El problema de la abscisión de frutos de naranja cobró importancia en 2017, pero ya existía este problema desde hace 15 años, lo cual ocasiona pérdidas en la producción del 20% en la zona Norte de Veracruz (Gómez et al., 2020). El ataque de patógenos: Colletotrichum spp, Botryodiplodia spp, Fusarium spp, Citrus tristeza virus (VTC), Candidatus Liberibacter asiaticus (CLas), Citrus exocortis viroid (CEVd) y Hop stunt viroid (HSVd); pueden influir, entre otros factores, en la caída de naranja. El experimento se llevó a cabo en la huerta `Grupo Los Gómez’ en el ejido de San Pablo, Papantla, Veracruz, México; en naranja ‘Valencia’ portainjerto ‘Agrio’ y en suelo tipo aluvión. El objetivo central de la investigación fue evaluar 15 insumos agroecológicos para disminuir la abscisión de naranja, 7 de estos insumos con micorrizas y el resto sin su aplicación. Los tratamientos con mejores resultados fueron: Agua de vidrio (1.25 %) + Micorriza; Optifer amino (0.5 %) + Micorriza; y EM (5 %) + Micorriza con menor porcentaje de caída de naranja con 4.97%, 5.79%, 6.10% respectivamente. 

Detection and molecular characterization of ‘Candidatus Liberibacter asiaticus’ and Citrus tristeza virus associated with citrus decline in Bhutan

2020 ◽  
Author(s):  
Dilip Kumar Ghosh ◽  
Amol Kokane ◽  
Sunil Kokane ◽  
Jigme Tenzin ◽  
Mrugendra G Gubyad ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Citrus Tristeza Virus ◽  
Citrus Greening ◽  
Candidatus Liberibacter Asiaticus ◽  
Field Samples ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Tristeza Virus ◽  
Citrus Tristeza

Citrus, mainly mandarin (Citrus reticulata Blanco) is an economically important fruit crop in Bhutan. Despite having favorable agro-climatic conditions for citrus cultivation, the early decline of fruit-bearing orchards coupled with low crop productivity is a major concern among the citrus growers. During the recent survey, an association of ‘Candidatus Liberibacter asiaticus’ (citrus greening) and citrus tristeza virus (CTV), either singly or as mixed infection in declined citrus trees was recorded in all four major citrus-growing districts viz. Tsirang, Dagana, Zhemgang and Sarpang. Using PCR-based diagnosis, a higher incidence of citrus greening (27.45 %) and tristeza (70.58 %) was observed in symptomatic field samples. Detection and characterization of ‘Ca. L. asiaticus’ (CLas) was performed based on 16S rDNA, Prophage gene, 50S ribosomal rplA-rplJ gene, and tandem repeats of CLIBASIA_01645 locus. Similarly, coat protein, p23, and p18 genes were used as genetic markers for the detection and characterization of Bhutanese CTV. The CLas isolates from Bhutan segregated into Class-II and III based on CLIBASIA_01645 locus, analogous to Indian isolates from the north-east region and Term-A based on CLIBASIA_05610 locus. CTV isolates of Bhutan were observed as closely related to VT strain, which is considered as most devastating. To the best of our knowledge, this is the first study on molecular characterization of CLas and CTV isolates and their association with citrus decline in Bhutan.

scholarly journals Resistance inducers and organic fertilizer in Citrus sinensis [L.] Osbeck infected with Candidatus Liberibacter asiaticus bacteria

2021 ◽  
Author(s):  
Yazmin Zapata Contreras ◽  
Eduardo Osorio Hernandez ◽  
José Hugo Silva Espinosa ◽  
Criseida Alhelí Saénz Pérez ◽  
Ma. Teresa de Jesús Segura Martínez
Keyword(s):  
Citrus Sinensis ◽  
Organic Fertilizer ◽  
Fruit Weight ◽  
Skin Thickness ◽  
Candidatus Liberibacter Asiaticus ◽  
Equatorial Diameter ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
Chlorophyll Index ◽  
Resistance Inducers

Objective: To evaluate the effect of three resistance inducers and an organic fertilizer on the titles of CandidatusLiberibacter asiaticus in Citrus sinensis (L.) Obseck cv. Valencia.Design/methodology/approach: The treatments consisted of Vacciplant Max and UPL-08, Fosetil aluminum. Likewise, BIO-FOM was applied on the periphery of the trees, with moisture for nutrients absorption. The evaluated variables were fruit weight, equatorial diameter, skin thickness, °BRIX, severity and chlorophyll, in each of the five treatments, which consisted of 20 repetitions.Results: The fruits of the trees treated with Vacciplant Max had lower skin thickness and a higher °BRIX. Also, the highest chlorophyll index was recorded with BIO-FOM fertilizer. However, none of the evaluated treatments significantly decreased the fruit harshness.Findings/conclusions: The best treatment against Huanglongbing was fosetyl aluminum which conferred greater fruit weight and diameter

scholarly journals Multi-omics Comparison Reveals Landscape of Citrus limon and Citrus sinensis Response to ‘Candidatus Liberibacter asiaticus’

2021 ◽  
pp. PHYTOFR-09-20-0
Author(s):  
Elizabeth L. Chin ◽  
John Ramsey ◽  
Surya Saha ◽  
Darya Mishchuk ◽  
Juan Chavez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Citrus Sinensis ◽  
Mass Spectrometry Analysis ◽  
Citrus Limon ◽  
Symptom Development ◽  
Candidatus Liberibacter Asiaticus ◽  
Spectrometry Analysis ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Comparison of the metabolic changes prior to symptom development upon infection with Candidatus Liberibacter asiaticus (CLas), the bacterium associated with citrus greening disease, between citrus hosts with different tolerances is lacking. The objective of this study was to compare the early response of Lisbon lemon (Citrus limon) and Washington navel orange (Citrus sinensis [L.] Osbeck), two citrus species commercially important to California, to CLas through graft inoculation. Here, we compare the transcriptome, proteome, and metabolome response, using RNA sequencing, liquid chromatography tandem mass spectrometry, and 1H nuclear magnetic resonance, respectively, from our two recently published studies examining the response of the lemon and navel oranges separately, and introduce new micronutrient data from inductively coupled plasma mass spectrometry analysis, focusing on lemons at 10 and 14 weeks postgrafting (wpg), and navels at 8 and 18 wpg, prior to symptom development. Several micronutrients accumulated in presymptomatic infected lemons compared with controls, whereas little change was observed in the navels. Photosynthesis proteins were substantially altered by CLas infection in navels, with fewer changes observed in lemons. The metabolome differed between control and infected navels throughout infection, although differences between control and infected lemons were identified only after symptom expression. Taken together, these findings highlight differences in response to CLas between two varieties with differing tolerances. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

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