Test strips for examining reactivity of fingerprints visualization methods on absorptive surfaces with amino acids

2015 ◽  
Vol 288 ◽  
pp. 104-107
Author(s):  
Tomasz Szczepański ◽  
◽  
Urszula Więckiewicz ◽  
Krzysztof Klemczak ◽  
◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Zinc Chloride ◽  
Fluorescence Emission ◽  
Routine Laboratory ◽  
Test Strips ◽  
Fluorescence Measurements ◽  
Acid Reaction ◽  
Acid Test ◽  
Amino Acid Concentrations

This article provides the results of research on the uniformity of amino acid test strips used with fluorescent DFO method. Ready to use amino acid test strips made of the absorptive material containing four reactive fields of exponentially decreasing amino acid concentrations, designed for DFO, ninhydrin, 1,2-indanedione methods were used in the study. The test strips were treated with DFO solution in order to measure the fluorescence emission level of DFO-amino acid reaction product for respective reaction fields. Based on the obtained results, the repeatability of emitted fluorescence measurements was observed, thus confirming the usability of test strips in DFO method verification of newly prepared reagents as well as the use of this method in routine laboratory work. Based on the findings, it should be assumed that test strips are also effective and suitable for verification of correctness of preparation of working solutions for the remaining amino acid based fingerprint visualization methods such as: ninhydrin, 1,2-indanedione, and 1,2-indanedione with zinc chloride.

scholarly journals Rabbit Carcasses for Use in Feline Diets: Amino Acid Concentrations in Fresh and Frozen Carcasses With and Without Gastrointestinal Tracts

2021 ◽  
Vol 7 ◽  
Author(s):  
Tammy J. Owens ◽  
Andrea J. Fascetti ◽  
C. Christopher Calvert ◽  
Jennifer A. Larsen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Protein Body ◽  
National Research Council ◽  
Body Water ◽  
Taurine Supplementation ◽  
Frozen Ground ◽  
Taurine Deficiency ◽  
Amino Acid Concentrations

Whole-prey diets for exotic feline species are common, and this practice has also increased in popularity for domestic cats. However, prior analyses of prey indicate possible essential amino acid inadequacy, and dilated cardiomyopathy from taurine deficiency was reported in cats fed whole ground rabbit. Crude protein, body water, and amino acid concentrations were evaluated in fresh and frozen ground rabbits with (n=10) or without (n = 10) gastrointestinal tracts. Amino acids were greater in fresh samples without gastrointestinal tracts (p < 0.05) except taurine, glycine, and cysteine. When normalized for protein content, only glutamate, alanine, methionine, isoleucine, tyrosine, lysine, histidine, and arginine were greater in fresh rabbits without gastrointestinal tracts (g/16 g N basis; p < 0.05). Freezing at −18°C for 30 days had no effect on crude protein or body water content. After freezing, only methionine was lower and only proline was higher when gastrointestinal tracts were omitted (g/16 g N basis; p < 0.05). Regardless, all essential amino acids except taurine exceeded Association of American Feed Control Officials and National Research Council nutrient recommendations for all feline life stages. In contrast, there was minimal impact of treatment on taurine concentrations. However, although feline taurine requirements for prey and other raw or fresh food diets remain undefined, none of the rabbit samples met any recommendation for taurine concentrations for commercial canned or dry extruded diets, ranging from 20 to 90% of the minimum values. Taurine supplementation is recommended when feeding rabbit to cats. Determination of taurine requirements of cats fed whole-prey diets is warranted.

Uptake and metabolism of amino acids by the dog liver perfused in situ

1962 ◽  
Vol 202 (3) ◽  
pp. 407-414 ◽  
Author(s):  
Rapier H. McMenamy ◽  
William C. Shoemaker ◽  
Jonas E. Richmond ◽  
David Elwyn
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Sharp Rise ◽  
Dog Blood ◽  
Dog Liver ◽  
Amino Acid Concentrations ◽  
Uptake And Metabolism

Dog livers were perfused in situ for periods up to 6 hr with dog blood recycled through a pump-oxygenator. An amino acid mixture was administered for 90 min. Concentrations of amino acids were determined at intervals of 30 min or more. Rates of uptake and metabolism were calculated. After the start of perfusion, there is a fall in most plasma amino acid concentrations and a reciprocal rise in liver amino acids. Addition of amino acids causes a sharp rise in plasma amino acids. There is a rapid uptake of most of the amino acids by liver, although the concentrations of amino acids in liver fail to rise appreciably. Notable exceptions are valine, leucine, and isoleucine. Uptake of amino acids stimulates: a) an increase in the rate of synthesis of urea which ultimately accounts for 90% of the metabolized amino acids; b) a net synthesis of ornithine; and c) net noncatabolic metabolism of amino acids which may in part be protein synthesis. The results support the view that the liver temporarily stores a part of ingested amino acids as proteins, and subsequently makes them available to other organs.

Gas Chromatography in Diagnostic Biochemistry of Abnormal Valine Metabolism

1973 ◽  
Vol 19 (9) ◽  
pp. 1040-1044 ◽  
Author(s):  
Hugh N Tucker ◽  
Samuel V Molinary
Keyword(s):  
Amino Acids ◽  
Gas Chromatography ◽  
Amino Acid ◽  
Biological Fluids ◽  
Sample Sizes ◽  
Diagnostic Aid ◽  
Chromatographic Techniques ◽  
Biochemical Evaluation ◽  
Amino Acid Concentrations ◽  
Chromatographic Evidence

Abstract Amino acids in biological fluids can be rapidly and accurately quantitated by gas chromatography. Modification of existing gas-chromatographic techniques allow as little as 25 µl of biological fluids to be analyzed, compatible with sample sizes easily obtained in pediatric studies. Simplified diagnostic biochemical evaluation of a suspected aminoaciduria can be begun at birth, or shortly after. Normal amino acid patterns are compared with those found in a patient with abnormal amino acid concentrations. Gas chromatographic evidence is presented as a diagnostic aid in the evaluation of a patient with abnormal valine metabolism.

scholarly journals 131 MODIFICATION OF AMINO ACID CONCENTRATIONS IN MEDIUM BY PIG EMBRYOS FROM THE ZYGOTE TO THE BLASTOCYST STAGE

2005 ◽  
Vol 17 (2) ◽  
pp. 216
Author(s):  
P. Booth ◽  
T. Watson ◽  
H. Leese
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Embryonic Development ◽  
Amino Acid Profile ◽  
Blastocyst Stage ◽  
Morula Stage ◽  
Amino Acid Profiles ◽  
The Difference ◽  
Amino Acid Concentrations

Pre-implantation embryos can produce and consume amino acids in a manner dependent upon stage of embryonic development (Partridge and Leese 1996 Reprod. Fert. Dev. 8, 945) that may also be predictive of subsequent viability (Houghton et al. 2002 Hum. Reprod. 17, 999). To examine these relationships in the pig, the appearance or depletion of 18 amino acids from a presumptive near-physiological mixture was determined by HPLC in porcine in vitro-produced embryos from the zygote to the blastocyst stage. Cumulus oocyte complexes derived from slaughterhouse prepubertal pig ovaries were matured for 40 h in modified TCM-199 before being fertilized (Day 0) with frozen thawed semen in tris-based medium. After 6 h, presumptive zygotes were denuded and cultured in groups of 20 in NCSU medium modified to contain a physiological mixture of 18 amino acids including 0.1 mM glutamine (NCSUaa). Groups of 2–10 embryos (dependent on stage) were removed on Day 0 (1 cell), Day 1 (2- and 4-cell), Day 4 (compact morula), and Day 6 (blastocyst) and placed in 4 μL NCSUaa for 24 h. After incubation, the embryos were removed and the medium analyzed by HPLC. Each stage was replicated 3–9 times. Since amino acid profiles of 2- and 4-cell embryos were not different, data were combined. Overall, arginine (1.19 ± 0.33), glutamine (0.78 ± 0.34) and threonine (0.05 ± 0.04) were significantly (P < 0.01) depleted from the medium whereas alanine (0.21 ± 0.1), glycine (0.20 ± 0.06), asparagine (0.13 ± 0.5), lysine (0.1 ± 0.03), isoleucine (0.08 ± 0.01), valine (0.05 ± 0.01), leucine (0.04 ± 0.02), phenylalanine (0.03 ± 0.01), and histidine (0.02 ± 0.04) significantly (P < 0.05) accumulated (mean of the 4 sampling timepoints; all values pmol/embryo/h ± SEM). The difference between amino acid accumulation and depletion (balance) was approximately equivalent between Day 0 and the morula stage although turnover (sum of depletion and accumulation) steadily decreased during this period from 3.1 on Day 0 to 1.35 pmol/embryo/h at the morula stage. However, at the blastocyst stage, turnover and balance increased to 6.32 and 2.42 pmol/embryo/h, respectively, i.e. net appearance occurred. Notable changes in amino acid profile during development included decreases in accumulation of asparagine, glutamate, and glycine in the medium and the depletion of glutamine over Days 0, 1, and 4, followed by reversal of these trends by Day 6. These data suggest that pig embryos can alter the accumulation and depletion rates of amino acids in a manner that is dependent on the specific amino acid and the stage of embryonic development. This work was supported by BBSRC.

Interorgan metabolism of amino acids in streptozotocin-diabetic ketoacidotic rat

1983 ◽  
Vol 244 (2) ◽  
pp. E151-E158 ◽  
Author(s):  
J. T. Brosnan ◽  
K. C. Man ◽  
D. E. Hall ◽  
S. A. Colbourne ◽  
M. E. Brosnan
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Amino Acid ◽  
Branched Chain Amino Acids ◽  
Diabetic Rats ◽  
Normal Rat ◽  
Diabetic Brain ◽  
Streptozotocin Diabetic Rats ◽  
Branched Chain ◽  
Amino Acid Concentrations

Amino acid concentrations in whole blood, liver, kidney, skeletal muscle, and brain were measured and arteriovenous differences calculated for head, hindlimb, kidney, gut, and liver in control and streptozotocin-diabetic rats. In the control rats, glutamine was released by muscle and utilized by intestine, intestine released citrulline and alanine, liver removed alanine, and the kidneys removed glycine and produced serine. In diabetic rats, the major changes from the pattern of fluxes seen in the normal rat were the release of many amino acids from muscle, with glutamine and alanine predominating, and the uptake of these amino acids by the liver. Glutamine removal by the intestine was suppressed in diabetes, but a large renal uptake of glutamine was evident. Branched-chain amino acids were removed by the diabetic brain, and consequently, brain levels of a number of large neutral amino acids were decreased in diabetes.

Effect of fertilization on free amino acid concentrations in black spruce and jack pine containerized seedlings

1987 ◽  
Vol 17 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Y. T. Kim ◽  
C. Glerum ◽  
J. Stoddart ◽  
S. J. Colombo
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Black Spruce ◽  
Jack Pine ◽  
Foliar Nitrogen ◽  
Containerized Seedlings ◽  
High Concentrations ◽  
Amino Acid Concentrations

Greenhouse-grown black spruce and jack pine container seedlings were fertilized weekly with a 20–8–20 fertilizer at four concentrations during the fall. Seedlings were sampled when 23 weeks old towards the end of the greenhouse cultural period to determine the effect of fertilization on the free amino acid concentrations. All amino acids, except tryptophan, showed significant increases in concentration with higher levels of fertilizer; the concentration of tryptophan decreased with increasing fertilizer concentration. Amino acids with the highest concentrations in black spruce were arginine, glutamic acid, and proline, while in jack pine, besides these three, aspartic acid and glutamine were also found in high concentrations. Black spruce had significantly higher amino acid concentrations than jack pine. The concentrations of certain free amino acids may be more sensitive indicators of seedling nitrogen status than total foliar nitrogen.

Interactions of neutral and acidic amino acids in renal tubular transport

1962 ◽  
Vol 202 (3) ◽  
pp. 577-583 ◽  
Author(s):  
William A. Webber
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Plasma Concentrations ◽  
Side Chain ◽  
Neutral Amino Acids ◽  
Acidic Amino Acids ◽  
Renal Tubular Reabsorption ◽  
Renal Tubular Transport ◽  
Renal Tubular ◽  
Amino Acid Concentrations

The effects of intravenous infusions of a variety of neutral and acidic amino acids on the plasma concentrations and excretions of naturally occurring amino acids were studied in dogs. Conventional clearance techniques were used, and the amino acid concentrations were determined by ion exchange column chromatography. Infusion of either l-glutamic acid or l-aspartic acid caused a gross increase in the plasma concentration and excretion of the other. Infusions of neutral amino acids including glycine, l-alanine, l-leucine, l-methionine, l-proline, and l-phenylalanine caused some minor changes in the endogenous plasma amino acid concentrations. They produced increases in the excretion of other neutral amino acids and, in some cases, of acidic and basic amino acids as well. In general, amino acids with long side chains were most effective in inhibiting reabsorption while cyclic side-chain compounds were less effective. There appear to be at least three somewhat separable mechanisms for renal tubular reabsorption of amino acids in dogs.

scholarly journals Effect of zinc deficiency on appetite and plasma amino acid concentrations in the rat

1981 ◽  
Vol 45 (1) ◽  
pp. 127-136 ◽  
Author(s):  
J. C. Wallwork ◽  
G. J. Fosmire ◽  
H. H. Sandstead
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Eating Habits ◽  
Plasma Amino Acid ◽  
Zn Deficiency ◽  
Feeding Cycle ◽  
Cyclic Feeding ◽  
Amino Acid Concentrations ◽  
Cell Volumes ◽  
Deficient Group

1. Levels of zinc in liver and plasma of the Zn-depleted rats fluctuated with the feeding cycle and were significantly higher at the bottom than at the top of the cycle. As Zn deficiency became more severe fluctuations in plasma Zn diminished. Concentrations of Zn in liver, in contrast to levels in plasma and femur, were not markedly lowered by day 15.2. In contrast to udlib.-fed (AL) and overnight-fasted (OF) controls, some pair-fed (PF) controls had elevated levels of Zn in liver and plasma.3. Intakes of water and food were sigdicantly correlated in Zn-deficient rats. Packed cell volumes were significantly higher for Zn-depleted than for AL and PF rats.4. Food intakes and plasma glucose concentrations were related in AL, OF and PF control rats but not in Zn-deficient rats.5. At day 15 of Zn deficiency the order of total plasma amino acid concentrations in the groups of rats was AL > Zn-deficient > OF > PF. Many of the differences between the AL and OF groups for individual plasma amino acids also appeared in the Zn-deficient group at the top and bottom of the feeding cycle. Differences in individual amino acid conmntrations at the top and bottom of the feeding cycle tended to be opposite in the PF and the Zn-ddicient group. Levels of tyrosine and tryptophan in plasma were correlated (P ≶ 0.05) with the cyclic feeding pattern of the Zn-deficient group; however, the ratios tryptophan or tyrosine: sum of other large neutral amino acids did not correlate significantly with the eating habits of Zndeficient rats.

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