scholarly journals Statistical optimization of an acid protease production by a local Aspergillus niger MH109542 using a medium based on decommissioned dates

2020 ◽  
pp. 68-76
Author(s):  
Malika Benkahoul ◽  
Amina Bramki ◽  
Aicha Belmessikh ◽  
Aicha Mechakra-Maza
Keyword(s):  
Aspergillus Niger ◽  
Yeast Extract ◽  
Corn Steep Liquor ◽  
Basal Medium ◽  
Extreme Environment ◽  
Fermentation Medium ◽  
Acid Protease ◽  
Protease Production ◽  
Plackett And Burman ◽  
Steep Liquor

The production of an acid protease by liquid fermentation is performed on a medium based on decommissioned dates by a local mold isolated from an extreme environment. The used mold is isolated from the thermal soil in Hammam Safsaf of Teleghma (Algeria). Phenotypic and molecular identification has shown that it is Aspergillus niger. This fungi strain exhibited exo-protease activity on milk agar. The fermentation medium based on decommissioned dates is enriched by other factors according to a statistical method; the plan of Plackett and Burman. This method (N = 8 experiments and N-1 factors) allowed the optimization of the enzyme production and the growth of the mold. The statistical analysis of the obtained results shows an increased enzyme activity (650.20U), in the presence of yeast extract and salts with probabilities p = 0.239 for the first and p = 0.190 for the second. Furthermore, the corn-steep-liquor (p = 0.229) and yeast extract (p = 0.053) present a significant effect on mold growth. In conclusion, the culture of A. niger on optimized medium gives good yields of biomass and proteolytic activity compared to the basal medium. These results are encouraging. In fact, the use of a cheap and available substrate such as decommissioned dates saves the cost price of fermentation on an industrial scale.

Optimization of medium components to improve the antimicrobial activity of a new Saharan actinobacterial strain Saccharothrix tamanrassetensis DSM 45947

2021 ◽  
Vol 16 (7) ◽  
pp. 35-47
Author(s):  
Keramane Leila Bakour ◽  
Hadjira Boudjella ◽  
Nouredinne Bouras ◽  
Florence Mathieu
Keyword(s):  
Antimicrobial Activity ◽  
Antibiotic Production ◽  
Corn Steep Liquor ◽  
Basal Medium ◽  
Fermentation Medium ◽  
Antimicrobial Activities ◽  
Potential Candidate ◽  
Medium Components ◽  
Wide Range ◽  
Steep Liquor

Saccharothrix tamanrassetensis DSM 45947 previously isolated from a Saharan soil sample and characterized as a new species, was found to be a potential candidate in the search for novel antibiotics. The strain was found to exhibit a strong antimicrobial activity against a wide range of bacteria and fungi. In this study, statistical methods were applied to optimize the fermentation medium for enhanced antimicrobial activity production. A basal medium supplemented with sucrose and corn steep liquor was used as the original medium for optimization experiments. Plackett-Burman design showed that sucrose, corn steep liquor and KH2PO4, were recognised to have significant effect on antimicrobial activity production. Response surface methodology with Box-Behnken design determined the optimal concentrations. Optimal values for maximum antibiotic production were as follows: 7 g L-1 of sucrose, 52 g L-1 of corn steep liquor and 0.9 g L-1 of KH2PO4. Under these conditions, antimicrobial activities of DSM 45947 showed a global increase by 32% against the bacterium methicillinresistant Staphylococcus aureus and 36% against the fungus Umbelopsis ramanniana. This study provided an economical and efficient method to cultivate Saccharothrix tamanrassetensis DSM 45947 for enhanced antimicrobial activity production.

PRODUCTION AND PROPERTIES OF 2,3-BUTANEDIOL: IX. THE EFFECT OF VARIOUS NUTRIENT MATERIALS ON THE FERMENTATION OF STARCH BY AEROBACILLUS POLYMYXA

1946 ◽  
Vol 24f (1) ◽  
pp. 29-38 ◽  
Author(s):  
Sybil B. Fratkin ◽  
G. A. Adams
Keyword(s):  
Wheat Flour ◽  
Yeast Extract ◽  
Corn Steep Liquor ◽  
Wheat Gluten ◽  
Wheat Starch ◽  
Whole Wheat Flour ◽  
Beneficial Effects ◽  
Whole Wheat ◽  
Steep Liquor ◽  
Starch Medium

Wheat starch is a poor medium for fermentation by Aerobacillus polymyxa. The solubles recovered from the separation of starch and gluten in patent flour enhance the fermentation but not as effectively as the similar fraction from whole wheat flour. Addition of supplements is necessary for a satisfactory yield of products in a reasonable length of time. Wheat gluten has no stimulatory effect but bran and shorts are both effective, the latter being slightly superior. An 8% starch medium fortified with the solubles from whole wheat required a 2.5% supplement of shorts to bring fermentation by A. polymyxa to 90% completeness in 72 hr.Of the various supplements tested, a 1% addition of malt sprouts proved to be the most effective, fermentation being 90% complete in 72 hr. Shorts, bran, Cerogras (dehydrated young oats), alfalfa, soya beans, yeast extract, and corn-steep liquor follow in order of decreasing effectiveness.The solubles from whole wheat when ashed have no beneficial effects on the fermentation of starch by A. polymyxa.

Organic Acid Production by Propionibacterium shermanii: Effect of pH, Temperature and Vitamin-Nitrogen Source

1997 ◽  
Vol 52 (3-4) ◽  
pp. 193-196 ◽  
Author(s):  
A. Quesada-Chanto ◽  
A . G. Schroeder ◽  
A. C. Schmid-Meyer ◽  
J. A. López ◽  
M. M. Silveira ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Propionic Acid ◽  
Yeast Extract ◽  
Acid Production ◽  
Ph Value ◽  
Corn Steep Liquor ◽  
Organic Acid Production ◽  
Propionibacterium Shermanii ◽  
Difco Yeast Extract ◽  
Steep Liquor

Abstract Production of propionic acid by Propionibacterium shermanii CDB 10014 was enhanced by a pH value of 6.5 and by temperatures in the range 35-37 °C. Depending on the type of yeast extract, succinic acid can be produced in higher proportions, with decreasing propionic acid yields. With respect to propionic acid production, Difco yeast extract has shown the best results when yeast extract preparations from other different suppliers were compared. To replace yeast extract by a cheaper vitamin-nitrogen source, corn-steep liquor was tested. A complete depletion of glucose was achieved, yielding a final propionic acid concentration of over 35 g/l. These results are even better than those obtained with Difco yeast extract and suggest the possibility of an economical process based on corn-steep liquor.

MICROBIAL PENTOSANASES: II. SOME FACTORS AFFECTING THE PRODUCTION OF PENTOSANASES BY BACILLUS PUMILUS AND BACILLUS SUBTILIS

1956 ◽  
Vol 2 (1) ◽  
pp. 28-38 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Yeast Extract ◽  
Wheat Bran ◽  
Soybean Meal ◽  
Corn Steep Liquor ◽  
Ammonium Phosphate ◽  
Water Soluble ◽  
High Rate ◽  
Mineral Salts ◽  
Ammonium Lactate ◽  
Steep Liquor

A number of carbohydrates and nitrogenous adjuncts were tested for their effect on the constitutive and adaptive pentosanases produced by Bacillus stibtilis and B. pumilus respectively in a medium containing biotin, ammonium phosphate, and other mineral salts. B. subtilis produced more enzyme with sulphite liquor than with any of the other carbohydrate sources tested. Next, in decreasing order of merit, were wheat bran, maltose, ribose, beet molasses, oat hulls, and pectin. Of the nitrogenous adjuncts tested, corn steep liquor, soybean meal, gelatin, gelysate, and ammonium lactate doubled the yield of enzyme whereas yeast extract, peptone, urea, and others were less effective. For B. pumilus the better carbohydrate sources, in decreasing order of merit, were wheat bran, water soluble pentosan of wheat flour, xylan, straw holo-cellulose, wheat straw, and sulphite liquor. Of the nitrogen sources, corn steep liquor was outstanding while casein, casitone, phytone, yeast extract, distillers' dried solubles, and soybean meal followed in decreasing order. A medium containing 6% wheat bran (20 mesh), 1% corn steep liquor neutralized with ammonia, 0.05% sodium chloride, and 0.05% calcium carbonate was devised for the production of pentosanase by B. pumilus. With this medium in shaken Erlenmeyer flasks, the enzyme was produced at a high rate between 12 and 40 hr.; thereafter the rate of production decreased. Maximum yields were obtained in 96 hr. A temperature of 26 °C. was more favorable for pentosanase production than higher temperatures.

Corn Steep Liquor as a Nutrition Adjunct for the Production of Aspergillus niger Lipase and Hydrolysis of Oils Thereof

2009 ◽  
Vol 57 (22) ◽  
pp. 10658-10663 ◽  
Author(s):  
N. G. Edwinoliver ◽  
K. Thirunavukarasu ◽  
S. Purushothaman ◽  
C. Rose ◽  
M. K. Gowthaman ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Corn Steep Liquor ◽  
Steep Liquor ◽  
Hydrolysis Of

scholarly journals Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation

2015 ◽  
Vol 12 (2) ◽  
pp. 307-316 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Aspergillus Niger ◽  
Specific Activity ◽  
Corn Steep Liquor ◽  
Optimum Conditions ◽  
Inulin Hydrolysis ◽  
Enzyme Productivity ◽  
Initial Ph ◽  
Quantitative Screening ◽  
Steep Liquor ◽  
Fungal Colony

Thirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase production. the most efficient isolate was AN20. The optimum condition for enzyme production from A. niger isolate was determined by busing a medium composed of sugar cane moisten with corn steep liquor 5;5 (v/w) at initial pH 5.0 for 96 hours at 30 0C . Enzyme productivity was tested for each of the yeast Kluyveromyces marxianus, the fungus A. niger AN20 and for a mixed culture of A. niger and K. marxianus. The productivity of A. niger gave the highest specific activity of 153 U/mg, as compared with K. marxianus which gave 86 U/mg.

Hydrolysis of Corncob Residue by Enzymes from Fungi C1067 and the Products Analysis by Capillary Electrophoresis

2011 ◽  
Vol 145 ◽  
pp. 68-72
Author(s):  
Jian Lu Ma ◽  
Xiao Min Fang ◽  
Xin Jiong Guo ◽  
Huan Fei Liu ◽  
Gui Fu Dai ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Citrate Buffer ◽  
Carbon And Nitrogen ◽  
Products Analysis ◽  
Steep Liquor ◽  
Hydrolysis Of ◽  
Assay Conditions

To achieve high enzymatic hydrolytic efficiency of corncob residue, one strain, Trichoderma C1067, with the enzyme activity of corncob residue as high as 4.5g/L glucose liberated per hour in 0.5mL crude cellulase and 0.5mL citric acid /sodium citrate buffer under the assay conditions, was carefully studied. The optimal carbon and nitrogen sources used in the fermentation medium are as follows: corncob residues 2.0%, wheat bran 1.5%, corncob 0.5%, rice straw 0.5%, peptone 0.1% and corn steep liquor 4%. The optimal conditions of saccharification for corncob are 55°C and pH 4.0. The sugars gained in the hydrolysate consist of glucose 4.1% and xylose 0.4% after enzymolysis for 72h, tested by capillary electrophoresis.

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