Evaluation of Biological Degraded Keratin for Biogas Production Using Dry Anaerobic Digestion System

2021 ◽  
Vol 2 (2) ◽  
pp. 81-89
Author(s):  
Sinta Setyaningrum ◽  
Regina J Patinvoh ◽  
Ronny Purwadi ◽  
Mohammad Taherzadeh
Keyword(s):  
Amino Acid ◽  
Anaerobic Digestion ◽  
Alternative Energy ◽  
Organic Waste ◽  
Biogas Production ◽  
Gas Production ◽  
Bacillus Sp ◽  
Hair Culture ◽  
Farming Industry ◽  
Hydrolysis Of

Anaerobic digestion is a methane gas production process that can be used as sustainable alternative energy. Anaerobic digestion utilized various types of organic waste as substrate for the reaction process. Keratin waste is an organic waste mainly produced from the poultry and farming industry. Pretreatment is usually required to hydrolyzed keratin protein complex as the amino acid is easily used as the substrate in anaerobic digestion reaction. Biological pretreatment was selected because it more energy saver and generating diverse types of amino acid monomers. Three types of keratins used in this research were feathers, wool, and hair. Culture of Bacillus sp. C4 were inoculated into keratins and incubated for 24 hours, 48 hours, and 72 hours. The chicken feathers produce the soluble protein as much as 7.23 mg/ml, 32.59 mg/ml and 45.99 mg/ml respectively, while the sheep wool produce 24.08 mg/ml, 36.73 mg/ml and 38.75 mg/ml respectively according to incubation time. Meanwhile, keratin hair cannot be degraded by Bacillus sp. C4 at all. Free ammonia formed by hydrolysis of proteins is suspected to be an inhibitor in the methanogenesis process, as total methane produced from degraded keratin only 256,6 ml C4/gr VS in 36 days retention time.

scholarly journals Sugarcane Bagasse as a Co-Substrate with Oil-Refinery Biological Sludge for Biogas Production Using Batch Mesophilic Anaerobic Co-Digestion Technology: Effect of Carbon/Nitrogen Ratio

Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Aiban Abdulhakim Saeed Ghaleb ◽  
Shamsul Rahman Mohamed Kutty ◽  
Gasim Hayder Ahmed Salih ◽  
Ahmad Hussaini Jagaba ◽  
Azmatullah Noor ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Sugarcane Bagasse ◽  
Organic Waste ◽  
Wastewater Treatment Plants ◽  
Biogas Production ◽  
Raw Materials ◽  
Oil Refinery ◽  
Methane Yield ◽  
Oil Refineries ◽  
Biological Sludge

Man-made organic waste leads to the rapid proliferation of pollution around the globe. Effective bio-waste management can help to reduce the adverse effects of organic waste while contributing to the circular economy at the same time. The toxic oily-biological sludge generated from oil refineries’ wastewater treatment plants is a potential source for biogas energy recovery via anaerobic digestion. However, the oily-biological sludge’s carbon/nitrogen (C/N) ratio is lower than the ideal 20–30 ratio required by anaerobic digestion technology for biogas production. Sugarcane bagasse can be digested as a high C/N co-substrate while the oily-biological sludge acts as a substrate and inoculum to improve biogas production. In this study, the best C/N with co-substrate volatile solids (VS)/inoculum VS ratios for the co-digestion process of mixtures were determined empirically through batch experiments at temperatures of 35–37 °C, pH (6–8) and 60 rpm mixing. The raw materials were pre-treated mechanically and thermo-chemically to further enhance the digestibility. The best condition for the sugarcane bagasse delignification process was 1% (w/v) sodium hydroxide, 1:10 solid-liquid ratio, at 100 °C, and 150 rpm for 1 h. The results from a 33-day batch anaerobic digestion experiment indicate that the production of biogas and methane yield were concurrent with the increasing C/N and co-substrate VS/inoculum VS ratios. The total biogas yields from C/N 20.0 with co-substrate VS/inoculum VS 0.06 and C/N 30.0 with co-substrate VS/inoculum VS 0.18 ratios were 2777.0 and 9268.0 mL, respectively, including a methane yield of 980.0 and 3009.3 mL, respectively. The biogas and methane yield from C/N 30.0 were higher than the biogas and methane yields from C/N 20.0 by 70.04 and 67.44%, respectively. The highest biogas and methane yields corresponded with the highest C/N with co-substrate VS/inoculum VS ratios (30.0 and 0.18), being 200.6 mL/g VSremoved and 65.1 mL CH4/g VSremoved, respectively.

scholarly journals Biogas potential of organic waste onboard cruise ships — a yet untapped energy source

2021 ◽  
Author(s):  
Kai Schumüller ◽  
Dirk Weichgrebe ◽  
Stephan Köster
Keyword(s):  
Energy Source ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Food Waste ◽  
Energy Demand ◽  
Organic Waste ◽  
Biogas Production ◽  
Biogas Yield ◽  
Cruise Ships ◽  
Detailed Presentation

AbstractTo tap the organic waste generated onboard cruise ships is a very promising approach to reduce their adverse impact on the maritime environment. Biogas produced by means of onboard anaerobic digestion offers a complementary energy source for ships’ operation. This report comprises a detailed presentation of the results gained from comprehensive investigations on the gas yield from onboard substrates such as food waste, sewage sludge and screening solids. Each person onboard generates a total average of about 9 kg of organic waste per day. The performed analyses of substrates and anaerobic digestion tests revealed an accumulated methane yield of around 159 L per person per day. The anaerobic co-digestion of sewage sludge and food waste (50:50 VS) emerged as particularly effective and led to an increased biogas yield by 24%, compared to the mono-fermentation. In the best case, onboard biogas production can provide an energetic output of 82 W/P, on average covering 3.3 to 4.1% of the total energy demand of a cruise ship.

scholarly journals Kinetics of Anaerobic Digestion of Dairy Fat Waste with Saponification Pre-Treatment

2019 ◽  
Vol 13 (2) ◽  
pp. 112 ◽  
Author(s):  
Rifki Wahyu Kurnianto ◽  
Wiratni Budhijanto ◽  
Rochim Bakti Cahyono
Keyword(s):  
Anaerobic Digestion ◽  
Lipid Content ◽  
Alternative Energy ◽  
Organic Waste ◽  
High Lipid Content ◽  
Dairy Fat ◽  
High Lipid ◽  
Pre Treatment ◽  
Kinetics Of ◽  
Floating Layer

Anaerobic digestion has been an attractive field of research in the era of energy crisis. Biogas, which is the product of anaerobic digestion, provides alternative energy, while at the same time it also prevents pollution due to organic waste accumulation. Among various organic wastes, dairy fat waste is a potential substrate for anaerobic digestion. Fat waste has high theoretical biogas potential because of its high lipid content. However, anaerobic digestion of organic waste with high lipid content is quite challenging. The main obstacle in anaerobic digestion of fat waste is its tendency to form insoluble floating layer on top of the liquid phase. This phenomenon hinders the access of hydrolytic bacteria to the substrate. Saponification is one of the methods to increase the solubility of the floating layer and hence to improve the availability of substrate for the bacteria. Saponification changes the lipid content into soap which has both polar and non-polar functional groups and the polar side will increase the solubility of the substrate in water. This study evaluated the effect of different dosage of base added as the reactant during saponification pre-treatment on the productivity of anaerobic digestion of dairy fat waste. The kinetics of the anaerobic digestion process was analyzed by mean of mathematical model. The variations of the alkaline dosages studied for saponification pre-treatment were 0.04 mol base/g sCOD; 0.02 mol base/g sCOD; and no pre-treatment for control reactor. This study proved that saponification increased the solubility of dairy fat waste. This result was confirmed by the hydrolysis constant value (kH) of 0.00782/day for reactor with saponification, which was twenty times of magnitude higher than the kH value of 0.00032/day in the reactor without saponification. However, the exposure to high pH during the saponification pre-treatment might somewhat inhibit indigenous acidogenic bacteria in the waste which results in lower methane yield in the reactors with saponification to be compared to the control reactor. A B S T R A KPeruraian anaerobik merupakan salah satu bidang riset yang sangat menarik perhatian dalam era krisis energi. Biogas tidak hanya menyediakan energi alternatif, tetapi juga dapat mencegah pencemaran akibat limbah organik. Limbah lemak susu adalah substrat yang potensial untuk proses peruraian anaerobik karena memiliki potensi biogas teoritis yang tinggi akibat kandungan lemaknya yang tinggi. Namun, peruraian anaerobik dari limbah organik dengan kandungan lemak yang tinggi memiliki tantangan tersendiri. Hambatan utama dalam peruraian anaerobik dari limbah lemak susu adalah kecenderungan untuk membentuk lapisan padatan yang tidak larut dan mengapung di bagian atas fase cair. Fenomena ini menghambat akses bakteri hidrolisis terhadap substrat. Saponifikasi adalah salah satu cara untuk meningkatkan kelarutan lapisan padatan tersebut, sehingga meningkatkan ketersediaan substrat untuk bakteri. Saponifikasi akan mengubah kandungan lemak menjadi sabun yang memiliki gugus fungsi polar maupun non-polar. Gugus fungsi yang bersifat polar akan meningkatkan kelarutan substrat dalam air. Studi ini mengevaluasi pengaruh dari berbagai dosis larutan basa yang ditambahkan sebagai reaktan selama perlakuan awal saponifikasi terhadap peruraian anaerobik limbah lemak susu. Kinetika proses peruraian anaerobik dianalisis dengan menggunakan model matematika. Variasi dosis yang diamati pengaruhnya untuk perlakuan awal saponifikasi adalah 0,04 mol basa/g sCOD; 0,02 mol basa/g sCOD; dan nol (tanpa perlakuan awal saponifikasi). Dari penelitian ini, terbukti bahwa saponifikasi berhasil meningkatkan kelarutan limbah lemak susu dan juga ditunjukkan oleh nilai konstanta hidrolisis (kH) 0,00782/hari lebih tinggi dua puluh kali lipat dibandingkan dengan nilai kH 0,00032/hari pada reaktor tanpa saponifikasi. Akan tetapi, penelitian ini juga mengindikasikan bahwa bakteri asidogenik bawaan substrat terhambat kinerjanya oleh paparan pH yang tinggi selama perlakuan awal saponifikasi berlangsung sehingga hasil gas metan yang diperoleh lebih rendah daripada reaktor kontrol.

scholarly journals Biogas Production from Anaerobic Digestion of Biodegradable Household Wastes

1970 ◽  
Vol 11 ◽  
pp. 167-172 ◽  
Author(s):  
Harka Man Lungkhimba ◽  
Amrit Bahadur Karki ◽  
Jagan Nath Shrestha
Keyword(s):  
Alternative Energy ◽  
Biogas Production ◽  
Gas Production ◽  
Biogas Plant ◽  
Waste Materials ◽  
Household Level ◽  
Fertilizer Value ◽  
Average Maximum ◽  
Cellulosic Waste ◽  
In The Beginning

This study focuses on production of biogas as an alternative energy by using biodegradable wastes (BWs) in view of solving waste management at household level. The research was conducted on ARTI model compact biogas plant of 1 m3 digester and 0.75 m3 gasholder in focusing the management of daily collected biodegradable wastes (1-2 kg) produced from households. Both laboratory and field analyses were carried out. Methane content in biogas was determined by Biogas Analyzer Gas Board-3200P. Average maximum of about 235 l gas was recorded per day with corresponding to 65 min/day burning hour with the gas flame of energy value 1.55 MJ/h. According to the plant owners, the burning period of the gas was approximately 2 h/day during the spring and pre-monsoon seasons. The average gas production (per kg) from fresh waste materials was approximately 60 l. The use of high moisture containing cellulosic waste materials and incomplete digestion due to lower digester temperature were the major causes of lower gas yield. The proportion of methane exceeded by carbondioxide in the beginning but then after gradually methane exceeded carbondioxide and reached 56.43% on an average. Although fertilizer value in terms of NPK increased gradually but it remained below 1% except K, which was found to be 1.22%. Simple payback periods of 4.81, 7.57 and 7.20 years were found in kerosene, firewood, and LPG substitutions respectively. Key words: alternative energy; biogas analyzer; compact biogas plant; methane; simple payback period DOI: 10.3126/njst.v11i0.4140Nepal Journal of Science and Technology 11 (2010) 167-172

scholarly journals Biogas Production from Rice Husk Waste by using Solid State Anaerobic Digestion (SSAD) Method

2018 ◽  
Vol 31 ◽  
pp. 02007 ◽  
Author(s):  
Hashfi Hawali Abdul Matin ◽  
Hadiyanto
Keyword(s):  
Anaerobic Digestion ◽  
Solid State ◽  
Rice Husk ◽  
Alternative Energy ◽  
Biogas Production ◽  
Lignin Content ◽  
Operating Conditions ◽  
Optimum Operating ◽  
Future Research ◽  
Continuous Systems

An effort to obtain alternative energy is still interesting subject to be studied, especially production of biogas from agriculture waste. This paper was an overview of the latest development of biogas researches from rice husk waste by Solid State Anaerobic Digestion (SSAD). The main obstacle of biogas production from rice husk waste was the lignin content which is very difficult degraded by microbes. Various pretreatments have been conducted, either physically, chemically as well as biologically. The SSAD method was an attractive option because of the low water content of rice husk waste. The biogas yield by SSAD method gave more attractive result compared to Liquid Anaerobic Digestion (LAD) method. Various studies were still conducted in batch mode laboratory scale and also has not found optimum operating conditions. Research on a larger scale such as bench and pilot scale with continuous systems will be an increase trend in the future research.

Study of Methane Production by High Temperature Anaerobic Fermentation of Chicken Manure and Stalks

2020 ◽  
Vol 14 (4) ◽  
pp. 551-557
Author(s):  
Yongku Li ◽  
Xiaomin Hu ◽  
Lei Feng
Keyword(s):  
High Temperature ◽  
Anaerobic Digestion ◽  
Production Rate ◽  
Methane Production ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Gas Production ◽  
Chicken Manure ◽  
Biogas Slurry ◽  
Production Rates

The changing parameters, as the biogas production rate, the methane production rate, the cumulative biogas amount, the cumulative methane amount, the biogas composition, pH etc. in high temperature anaerobic fermentation of chicken manure and stalks were analyzed by experiments with different mass ratios of chicken manure or livestock manure and stalks with a high C/N ratio. The methane production mechanism of high temperature anaerobic digestion of chicken manure and stalks was discussed in detail. It showed that not only the biogas production rates but also the methane production rates of R1–R7 demonstrated the trend of initial increase and then decrease after 50 d of high temperature anaerobic digestion. Besides, the gas production of R1 with pure chicken manure stopped on the 30th d of the reaction. The gas production of other groups R2–R7 also stopped on the corresponding 34th, 36th, 36th, 37th, 37th, and 37th day, respectively. At the end of the reaction, the cumulative biogas amounts and the cumulative methane amounts of R1–R7 were 411.58 and 269.54, 459.91 and 314.41, 425.32 and 294.11, 401.85 and 272.54, 382.63 and 257.07, 363.04 and 218.16, and 257.15 and 160.10 N ml/(g VS). The biogas slurry pH of R1–R7 all demonstrated a trend of initial decrease and then increase, e. g., pH of R2 reached the minimum of 5.94 on the 5th day. pH values of other groups were between 6.01 and 6.39. After the addition of 4 g of sodium bicarbonate on the 7th day, biogas slurry pH of R1–R7 all increased. pH was maintained between 7.16 and 7.44 until the end of the reaction.

Mesophilic Anaerobic Digestion of Dairy Manure and Vegetable Waste Using Batch Reactor

2014 ◽  
Vol 1008-1009 ◽  
pp. 121-126
Author(s):  
Lin Jun Shi ◽  
Wen Lan Liu ◽  
Hui Fen Liu ◽  
Wei Yu Zhang ◽  
Li Tong Ban
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Batch Reactor ◽  
Theoretical Data ◽  
Gas Production ◽  
Dairy Manure ◽  
Experimental Results ◽  
Vegetable Waste ◽  
Start Up ◽  
Waste Mixture

Anaerobic digestion of single dairy manure, single vegetable waste, mixture of dairy manure and vegetable waste was conducted to produce biogas. Startup characteristic, leachate parameters and inoculation amount were investigated. The experimental results showed that anaerobic digestion can start up quickly with acclimated thickening sludge as inoculation sludge and 30% was appropriate inoculation percentage. Digestion of single dairy manure and mixture of dairy manure and vegetable waste appeared better buffering ability with higher alkalinity than single vegetable waste. Compared to single digestion of dairy manure or vegetable waste, mixture of dairy manure and vegetable waste is more suitable for anaerobic digestion. Under the conditions of TS=10% and T=(36±1)°C, cumulative biogas production of mixture of dairy manure and vegetable waste is 5281 mL during the period of 30 days and average daily gas production is about 176 mL. These results could provide theoretical data for practical biogas engineering.

Comparison of dry and wet digestion for solid waste

2003 ◽  
Vol 48 (4) ◽  
pp. 15-20 ◽  
Author(s):  
L. Luning ◽  
E.H.M. van Zundert ◽  
A.J.F. Brinkmann
Keyword(s):  
Anaerobic Digestion ◽  
Organic Waste ◽  
Gas Production ◽  
Organic Loading Rate ◽  
Inert Material ◽  
Environmental Standards ◽  
Dry Process ◽  
Wet Process ◽  
Crucial Difference ◽  
Mechanical Separation

To reduce the amount of MSW going to landfills a number of technologies have been developed. Two main types of anaerobic digestion processes are distinguished for MSW organic waste, which are generally referred to as “wet” (10-15% DM) and “dry” (24-40% DM) anaerobic digestion processes. The input is conditioned to the appropriate DM content by adding process water as required. This article compares a full-scale dry process, Valorga in La Coruña, Spain and a wet process, Vagron in Groningen, The Netherlands. A crucial difference is that the Vagron facility applies a washing step prior to the AD process to remove inert materials for re-use and to prevent damage of the installation. In the Valorga facility the organic fraction of the mechanical separation process is fed directly to the AD process. Both processes can be considered proven technology. Specific gas production is practically identical. Waste water production is higher in the wet process, as is to be expected, but this is compensated by a smaller amount of digestate to be disposed of and the separation of inert materials suitable for recycling. The organic loading rate for the Vagron process appears to be higher and the required reactor volume smaller in comparison to the dry Valorga process. The applicability of AD processes is strongly determined by the environmental standards set for the products from digestion. The German standards for digestate result in a lower potential for recycling of inert material separated from organic MSW.

scholarly journals Evaluation of acidogenic sludge from anaerobic reactors running at low solids retention times to reduce sludge generation and enhance biogas production

Water SA ◽  
2019 ◽  
Vol 45 (4 October) ◽  
Author(s):  
Wilza Da Silva Lopes ◽  
Ysa Helena Diniz Morais de Luna ◽  
Jose Tavares de Sousa ◽  
Wilton Silva Lopes ◽  
Valderi Duarte Leite
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Kinetic Constant ◽  
Oxygen Demand ◽  
Gas Production ◽  
Primary Sludge ◽  
Anaerobic Reactors ◽  
Solids Retention ◽  
Improved Performance ◽  
Pre Treatment

ABSTRACT   Sludges generated in the biological processing of sewage are complex mixtures, the constituents of which pose risks to public health and the environment. Anaerobic digestion is considered the most sustainable option for treating sludge because it offers the possibility of generating biogas. The aim of this study was to compare the quantities, properties, biodegradabilities and biochemical methane potentials (BMP) of primary sludge (PS) generated by a primary decanter with acidogenic sludges produced by upflow anaerobic (UA) reactors operating at solids retention times (SRTs) of 2, 4, 6 and 8 days (Samples S2, S4, S6 and S8, respectively). Sludges from both pre-treatments were submitted to alkaline solubilization in order to determine the efficiency of the process in disrupting extracellular complexes. Based on the levels of total solids (TS) present, the primary decanter was found to generate higher quantities of excess sludge (yield of 3.1 gTS∙d-1) than UA reactors operating at low SRTs (yields in the range 1.69 to 0.64 gTS∙d-1). The concentrations of dissolved materials in PS and Samples S2 and S8 were considerably higher after alkaline solubilization, with respective increases of 8, 14 and 28-fold in dissolved organic carbon, 12, 20 and 40-fold in chemical oxygen demand, 25, 31 and 59-fold in proteins, and 17, 21 and 63-fold in carbohydrates. In addition, the BMP value for S8 was some 13% higher than that recorded for PS while the kinetic constant for gas production by S8 was 1.8-fold greater than that of PS. It is concluded that a pre-treatment combining anaerobic digestion at low SRT and alkaline solubilisation would lead to improved performance in subsequent stages of anaerobic digestion and, consequently, increased efficiency in biogas production.

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