scholarly journals Генотипирование сортов винограда селекции Института «Магарач» на основе анализа аллельного полиморфизма SSR локусов

2019 ◽  
pp. 289-293
Author(s):  
Svetlana Gorislavets ◽  
Vitalii Volodin ◽  
Gennadii Spotar ◽  
Valentina Risovannaya ◽  
Yakov Alekseev
Keyword(s):  
Genetic Variation ◽  
Simple Sequence Repeats ◽  
Microsatellite Loci ◽  
Molecular Genetic ◽  
Information Value ◽  
International Standards ◽  
Agricultural Crops ◽  
Ssr Analysis ◽  
Short Period ◽  
Simple Sequence

Обязательными условиями успешного сохранения и использования различных сортов сельскохозяйственных культур является идентификация и контроль генетической изменчивости сортов, для изучения которой используются различные методы, в том числе методы молекулярно-генетического анализа. В связи с быстрым развитием селекции, ежегодно появляются десятки новых сортов винограда, требующих паспортизации. Молекулярные маркеры могут способствовать подбору родительских пар для скрещивания, повышению точности и ускорению селекционного процесса, так как идентификация исходного материала с использованием молекулярных маркеров и анализ результатов скрещивания могут быть выполнены в достаточно короткий период. К наиболее информативным молекулярным маркерам относятся микросателлитные маркеры, основанные на анализе простых повторяющихся повторов (simple sequence repeats, SSR). Анализ полиморфизма SSR локусов позволяет изучить генетическую изменчивость сельскохозяйственных культур на уровне генома. Цель нашего исследования - генотипирование, оценка аллельного разнообразия и ДНК-паспортизация ряда сортов винограда селекции Института «Магарач» на основе SSR анализа. Основной метод, использованный в работе, - полимеразная цепная реакция (ПЦР) и фрагментный анализ продуктов ПЦР на генетическом анализаторе ABI 3130. В результате фрагментного анализа были генотипированы 8 селекционных сортов Института «Магарач» по 9 ядерным микросателлитным локусам (nSSR). Размеры аллелей оценены с помощью программы Gene Mapper v. 4.0. Полиморфизм микросателлитных локусов и генетическое разнообразие рассчитано с использованием программы Popgene (v. 1.32). Сравнительный анализ nSSR профилей ДНК изученных сортов позволил установить, что все сорта имеют уникальные профили. Всего идентифицировано 69 аллелей, среднее число аллелей - 7,67 аллелей/локус. На основании размеров аллелей составлены индивидуальные молекулярно-генетические паспорта в соответствии с международными стандартами.Identification and control of genetic variation of different varieties of agricultural crops enter as prerequisites for their efficient conservation and use. Genetic variation is investigated by means of a wide set of methods, including those relying on molecular-genetic analysis. Every year, dozens of new breedings of grapevine come into being, and their passportization is necessary. Molecular markets can promote selection of parent pairs for crossing, improve efficiency of breeding and accelerate the process as enable both identification of the initial material and analysis of crossing results to be done in a sufficiently short period of time. The highest information value is associated with microsatellite markers consisting of simple sequence repeats (SSR). Analysis of polymorphism of SSR loci allows to investigate genetic variation of agricultural crops at the level of genome. The goals of the study were to conduct genotyping of a number of grape breedings developed by the Institute Magarach, to assess their allelic diversity and to achieve DNA passportization based on SSR analysis. Polymerase chain reaction (PCR) and fragment analysis of PCR products with the use of a genetic analizer ABI 3130 were the main methods the study relied upon. As a result of the aforesaid analysis, eight new breedings of the Institute were genotyped for nine nuclear microsatellite loci (nSSR). The sizes of alleles were assessed using Gene Mapper v. 4.0 software. Popgene (v. 1.32) software was used to calculate polymorphism of microsatellite loci and genetic diversity. A comparative nSSR-analysis of DNA-profiles of the study varieties indicated that all of them had unique profiles. A total of 69 alleles were identified, with 7.67 alleles per locus on an average. Based on the sizes of the alleles, individual molecular-genetic passports of the varieties were made, in accordance with international standards.

scholarly journals Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Uslan Uslan ◽  
NUR JANNAH
Keyword(s):  
Genetic Diversity ◽  
Zea Mays ◽  
Genetic Variation ◽  
Genetic Distance ◽  
Simple Sequence Repeats ◽  
Zea Mays L ◽  
Sample Collection ◽  
High Genetic Diversity ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Abstract. Uslan, Jannah N. 2020. Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas 21: 1208-1214. Corn (Zea mays L.) is one of the most important food crops in Indonesia. However, the studies described their genetic variation is relatively poor. Therefore, the aim of this study was to analyze the genetic diversity of local corn cultivars from South Amarasi, Kupang District, East Nusa Tenggara (NTT), Indonesia using ISSR markers. The sampling was conducted in 4 different sites in Sub-district of Amarasi, Kupang District. A total of 11 corn cultivars from Sub-district of South Amarasi was collected. DNA isolation was performed by using CTAB Method. Clustering analysis was conducted on MSVP 3.2 software. It was shown that all ISSR-primers used (UBC 811, UBC 814 and UBC 824) were successfully produced polymorphic bands and represents the high genetic diversity of the local corn cultivars. The genetic distance index indicated that several corn cultivars from two different populations were geographically unclustered, although there are samples from several populations that have a low genetic distance. The genetic variation index also showed high genetic diversity among the populations. Further research on the exhaustive sample collection was needed to give an insight into the genetic diversity of local corn cultivars (Zea mays L.) from South Amarasi, Indonesia. Please write implementation of this research

scholarly journals Microsatellites for coriander crop: A cross species amplification

2018 ◽  
Vol 33 (00) ◽  
Author(s):  
Sharda Choudhary
Keyword(s):  
Simple Sequence Repeats ◽  
Microsatellite Loci ◽  
Cost Effective ◽  
The Public ◽  
Microsatellites Markers ◽  
Band Size ◽  
Cost Effective Approach ◽  
Breeding And Genetics ◽  
Simple Sequence ◽  
New Crop

Development of microsatellites (Simple Sequence Repeats) is an expensive and time consuming process for new crop like coriander, but this can be easily achieved by transferring the microsatellite loci from the same family/genus/species which is a cost-effective approach for development of microsatellites in lesser studied crops like coriander. For cross-species investigation presently carrot and celery microsatellites are available on the public domain. We examined the transferability of hundred carrot (Daucuscarota) microsatellite loci to coriander (Coriandrum sativum L.). Thirty nine percent primer (39/100) amplified for coriander. Out of 39 microsatellites loci, 35 primers were monomorphic and specific to carrot primers, showing approximately the same size of microsatellites loci as reported for carrot. Four SSRs were polymorphic; seven primer pairs were non-specific which were deviating for band size from the source of microsatellites. These microsatellites markers should provide a powerful tool for coriander breeding and genetics.

Genetic diversity and molecular markers of five snapper species

2007 ◽  
Vol 4 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Liu Li ◽  
Liu Chu-Wu
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Microsatellite Loci ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
The Other ◽  
Information Contents ◽  
The Mean ◽  
Simple Sequence

AbstractIn order to protect and develop valuable snappers (Lutjanus spp.), genetic diversity and molecular markers of five species (Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus) were detected and analysed using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) techniques. The polymorphic loci ratio (P) (86.00–92.11%), the mean intraspecies genetic distances (D) (0.1775–0.3431) and the intraspecies genetic diversity indexes (Hi) (0.1022–0.1634) were calculated using the RAPD technique. The genetic diversities of L. fulviflamma and L. vitta were richest in terms of P, and D and Hi, respectively. The results of SSR showed that low effective numbers of alleles (1.7893–3.6591), medium average heterozygosities (0.332–0.676) and medium polymorphism information contents (PIC) (0.302–0.641) occurred in five species of snappers, indicating comparatively rich genetic diversity among these fish. Nine molecular markers in the products amplified by primers OPA8 and OPP10, and six molecular markers in 11 microsatellite loci were found to be useful as specific markers to identify five species of snappers. Two neighbour-joining (NJ) dendrograms based on the results of RAPD and SSR suggested that L. stellatus and L. sebae are closely related and clustered in one branch, with L. vitta, L. fulviflamma and L. fulvus in the other.

scholarly journals Genetic Variation in Sorghum Germplasm from Sudan, ICRISAT, and USA Assessed by Simple Sequence Repeats (SSRs)

Crop Science ◽  
2005 ◽  
Vol 45 (4) ◽  
pp. 1636-1644 ◽  
Author(s):  
A. H. Abu Assar ◽  
R. Uptmoor ◽  
A. A. Abdelmula ◽  
M. Salih ◽  
F. Ordon ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Simple Sequence Repeats ◽  
Simple Sequence ◽  
Sorghum Germplasm

scholarly journals Molecular genetics and SSR markers as a new practice in farm animal genomic analysis for breeding and control of disease disorders

2013 ◽  
Vol 29 (3) ◽  
pp. 405-429 ◽  
Author(s):  
A. Teneva ◽  
K. Dimitrov ◽  
Caro Petrovic ◽  
M.P. Petrovic ◽  
I. Dimitrova ◽  
...  
Keyword(s):  
Genomic Selection ◽  
Molecular Genetics ◽  
Genetic Markers ◽  
Simple Sequence Repeats ◽  
Animal Health ◽  
Molecular Genetic ◽  
Genomic Analysis ◽  
Animal Breeding ◽  
Farm Animal ◽  
Simple Sequence

Molecular genetics investigates the genetic makeup of individuals at the DNA level. That includes the identification and mapping of molecular genetic markers and genetic polymorphisms. Molecular genetic markers (DNA markers) are one of the most powerful means for the genomic analysis and allow the connection of hereditary traits with genomic variation. Molecular marker technology has developed rapidly over the last decade and two shapes of specific DNA based marker, Simple Sequence Repeats (SSRs), also known as microsatellites, and Single Nucleotide Polymorphisms (SNPs) prevail applications in modern genetic analysis. Genomic simple sequence repeats (SSRs, microsatellites) have been used for a variety of purposes, including gene tagging, physical mapping, genome mapping, estimation of genetic diversity, phylogenetic and conservation genetic purposes in farm animal breeding. SSR analyses are applied successfully in parentage verification and pedigree analysis, as disease markers and to locate the mutation in genetic disorders in livestock animals. The ultimate use of SSRs markers is for mapping quantitative trait loci (QTL), marker assisted selection (MAS) in order to practice genomic selection and improve the farm animal health. Developments in ?omics? technologies, such as genomic selection, may help overcome several of the limitations of traditional breeding programmes and will be especially beneficial in breeding for lowly heritable disease traits that only manifest themselves following exposure to pathogens or environmental stressors in adulthood. The current paper provides a brief overview of the present - day application of microsatellites markers in animal breeding and make significant contribution to the overall farm animal health and resistance to disease.

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