scholarly journals Enhanced Biosynthesis of Gossypol in Cell Suspension Culture of Gossypium hirsutum through Elicitation by Water Soluble Carbon Nanotubes

2017 ◽  
Vol 07 (01) ◽  
Author(s):  
Sameer Dixit ◽  
Gourav Jain ◽  
Surjeet Kumar Arya ◽  
Praveen C. Verma

1988 ◽  
Vol 7 (4) ◽  
pp. 266-269 ◽  
Author(s):  
L. Curt Blair ◽  
Chris J. Chastain ◽  
Jack M. Widholm


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
SR Ashtiani ◽  
T Hasanloo ◽  
MR Bihamta




2021 ◽  
Vol 26 (3) ◽  
pp. 456-467
Author(s):  
Yan-Ping Li ◽  
Dao-Bang Tang ◽  
Xiao-Qiang Wang ◽  
Meng Wang ◽  
Qing-Feng Zhang ◽  
...  


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.



2014 ◽  
Vol 38 ◽  
pp. 76-82 ◽  
Author(s):  
Zohreh JALALPOUR ◽  
Leila SHABANI ◽  
Ladan AFGHANI ◽  
Majid SHARIFI-TEHRANI ◽  
Sayed-Asadollah AMINI


2000 ◽  
Vol 193 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Remigius Uchenna Agu ◽  
Mark Jorissen ◽  
Tom Willems ◽  
Guy Van den Mooter ◽  
Renaat Kinget ◽  
...  


2008 ◽  
Vol 3 (11) ◽  
pp. 1398-1406 ◽  
Author(s):  
Stephanus J. Ferreira ◽  
Jens Kossmann ◽  
James R. Lloyd ◽  
Jan-Hendrik Groenewald


2016 ◽  
Vol 226 ◽  
pp. 44-53 ◽  
Author(s):  
Jae-Wan Jung ◽  
Nan-Sun Kim ◽  
Seon-Hui Jang ◽  
Yun-Ji Shin ◽  
Moon-Sik Yang


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