scholarly journals Uji beberapa dosis ekstrak buah mengkudu (Morinda citrifolia l.) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Victorson L. Zega ◽  
Pemsi M. Wowor ◽  
Christi Mambo
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Folk Medicine ◽  
Morinda Citrifolia ◽  
Fruit Extract ◽  
Lower Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lower Blood

Abstract: Herbal and natural products of folk medicine have been used for centuries in every culture throughout the world. One of folks medicine that has been used in Indonesia is the noni fruit. According to research conducted by I Ketut Adnyana, et al, there is a decrease in blood glucose levels from noni fruit extract with a dose of 500 mg/kg BW and 1000 mg/kg BW. This research aims to know the minimal dose of noni fruit (Morinda citrifolia L.) extract against a decrease in blood glucose levels in Wistar rats (Rattus norvegicus) induced alloxan. This study used 15 Wistar rats with diabetic induced by 130 mg/kg BW alloxan intraperitoneal. The rats were randomly divided into 5 groups; each group were given Aquades, Insulin Novomix 0,4 IU/100 gr BW, Noni fruit extract 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW. The result data obtained by the results of blood glucose levels evaluation on each rats on the first, second, and second day before the treatment after 6 hours, 12 hours, 18 hours, and 24 hours. Data from the measurement showed that noni fruit extracts with a dose of 125 mg/kg BW has the effect to lower blood glucose levels in Wistar rats induced alloxanKeywords: morinda citrifolia L., noni fruit, blood glucose levels, alloxan Abstrak: Produk herbal dan alami dari obat tradisional telah digunakan selama berabad-abad di setiap kebudayaan di seluruh dunia. Salah satu obat tradisional yang sudah dimanfaatkan di Indonesia ialah buah mengkudu (Morinda citrifolia L.). Menurut penelitian yang dilakukan oleh I Ketut Adnyana, dkk terdapat penurunan kadar glukosa darah dari ekstrak buah mengkudu dengan dosis 500 mg/kg BB dan 1000 mg/kg BB. Penelitian ini bertujuan untuk mengetahui dosis minimal dari ekstrak buah mengkudu (Morinda citrifolia L.) terhadap penurunan kadar glukosa darah pada tikus Wistar (Rattus norvegicus) yang diinduksi aloksan. Tikus Wistar sejumlah 15 ekor diinduksi dengan aloksan intraperitoneal 130 mg/kg BB dan dibagi secara acak dalam 5 kelompok penelitian yaitu kelompok perlakuan dengan Aquades, Insulin Novomix 0,4 IU/100 gr BB, ekstrak buah mengkudu 125 mg/kg BB, 250 mg/kg BB dan 500 mg/kg BB. Data yang diperoleh dari hasil pemeriksaan kadar glukosa darah pada semua kelompok tikus Wistar pada hari pertama, hari kedua, dan hari kedua jam ke-0, 6, 12,18 dan 24. Hasil penelitian menunjukkan bahwa ekstrak buah mengkudu dengan dosis 125 mg/kg BB sudah memiliki efek untuk menurunkan kadar glukosa darah pada tikus Wistar yang diinduksi aloksan. Kata kunci: morinda citrifolia L., buah mengkudu, kadar glukosa darah, aloksan.

scholarly journals Exogenous Ketones Lower Blood Glucose Level in Rested and Exercised Rodent Models

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2330 ◽  
Author(s):  
Ari ◽  
Murdun ◽  
Koutnik ◽  
Goldhagen ◽  
Rogers ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Rodent Models ◽  
Lower Blood Glucose ◽  
Post Exercise ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lower Blood Glucose Level ◽  
Lower Blood

Diseases involving inflammation and oxidative stress can be exacerbated by high blood glucose levels. Due to tight metabolic regulation, safely reducing blood glucose can prove difficult. The ketogenic diet (KD) reduces absolute glucose and insulin, while increasing fatty acid oxidation, ketogenesis, and circulating levels of β-hydroxybutyrate (βHB), acetoacetate (AcAc), and acetone. Compliance to KD can be difficult, so alternative therapies that help reduce glucose levels are needed. Exogenous ketones provide an alternative method to elevate blood ketone levels without strict dietary requirements. In this study, we tested the changes in blood glucose and ketone (βHB) levels in response to acute, sub-chronic, and chronic administration of various ketogenic compounds in either a post-exercise or rested state. WAG/Rij (WR) rats, a rodent model of human absence epilepsy, GLUT1 deficiency syndrome mice (GLUT1D), and wild type Sprague Dawley rats (SPD) were assessed. Non-pathological animals were also assessed across different age ranges. Experimental groups included KD, standard diet (SD) supplemented with water (Control, C) or with exogenous ketones: 1, 3-butanediol (BD), βHB mineral salt (KS), KS with medium chain triglyceride/MCT (KSMCT), BD acetoacetate diester (KE), KE with MCT (KEMCT), and KE with KS (KEKS). In rested WR rats, the KE, KS, KSMCT groups had lower blood glucose level after 1 h of treatment, and in KE and KSMCT groups after 24 h. After exercise, the KE, KSMCT, KEKS, and KEMCT groups had lowered glucose levels after 1 h, and in the KEKS and KEMCT groups after 7 days, compared to control. In GLUT1D mice without exercise, only KE resulted in significantly lower glucose levels at week 2 and week 6 during a 10 weeks long chronic feeding study. In 4-month and 1-year-old SPD rats in the post-exercise trials, blood glucose was significantly lower in KD and KE, and in KEMCT groups, respectively. After seven days, the KSMCT group had the most significantly reduced blood glucose levels, compared to control. These results indicate that exogenous ketones were efficacious in reducing blood glucose levels within and outside the context of exercise in various rodent models of different ages, with and without pathology.

scholarly journals Protective effect of cyanidin-3-O-glucoside on neonatal porcine islets

2017 ◽  
Vol 235 (3) ◽  
pp. 237-249 ◽  
Author(s):  
Chao Li ◽  
Bin Yang ◽  
Zhihao Xu ◽  
Eric Boivin ◽  
Mazzen Black ◽  
...  
Keyword(s):  
Drinking Water ◽  
Blood Glucose ◽  
Related Factor ◽  
Post Transplantation ◽  
Lower Blood Glucose ◽  
Glucose Levels ◽  
Porcine Islets ◽  
Blood Glucose Levels ◽  
Lower Blood ◽  
Neonatal Porcine Islets

Oxidative stress is a major cause of islet injury and dysfunction during isolation and transplantation procedures. Cyanidin-3-O-glucoside (C3G), which is present in various fruits and vegetables especially in Chinese bayberry, shows a potent antioxidant property. In this study, we determined whether C3G could protect neonatal porcine islets (NPI) from reactive oxygen species (H2O2)-induced injury in vitro and promote the function of NPI in diabetic mice. We found that C3G had no deleterious effect on NPI and that C3G protected NPI from damage induced by H2O2. Significantly higher hemeoxygenase-1 (HO1) gene expression was detected in C3G-treated NPI compared to untreated islets before and after transplantation (P < 0.05). Western blot analysis showed a significant increase in the levels of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphatidylinositol 3-kinase (PI3K/Akt) proteins in C3G-treated NPI compared to untreated islets. C3G induced the nuclear translocation of nuclear erythroid 2-related factor 2 (NRF2) and the significant elevation of HO1 protein. Recipients of C3G-treated NPI with or without C3G-supplemented drinking water achieved normoglycemia earlier compared to recipients of untreated islets. Mice that received C3G-treated islets with or without C3G-supplemented water displayed significantly lower blood glucose levels at 5–10 weeks post-transplantation compared to mice that received untreated islets. Mice that received C3G-treated NPI and C3G-supplemented drinking water had significantly (P < 0.05) lower blood glucose levels at 7 and 8 weeks post-transplantation compared to mice that received C3G-treated islets. These findings suggest that C3G has a beneficial effect on NPI through the activation of ERK1/2- and PI3K/AKT-induced NRF2-mediated HO1 signaling pathway.

Peripherally active dextromethorphan derivatives lower blood glucose levels by targeting pancreatic islets

2021 ◽  
Author(s):  
Okka Scholz ◽  
Silke Otter ◽  
Alena Welters ◽  
Laura Wörmeyer ◽  
Jurij Dolenšek ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Pancreatic Islets ◽  
Lower Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lower Blood

scholarly journals Glycemic control is associated with lower odds of mortality and successful extubation in severe COVID-19

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jay M. Pescatore ◽  
Juan Sarmiento ◽  
Ruben A. Hernandez-Acosta ◽  
Britt Skaathun ◽  
Nancy Quesada-Rodriguez ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Patient Characteristics ◽  
Lower Blood Glucose ◽  
Lower Odds ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lower Blood ◽  
Peak Blood ◽  
Peak Blood Glucose ◽  
Successful Extubation

Abstract Context Corticosteroids, specifically dexamethasone, have become the mainstay of treatment for moderate to severe COVID-19. Although the RECOVERY trial did not report adverse effects of corticosteroids, the METCOVID (Methylprednisolone as Adjunctive Therapy for Patients Hospitalized with COVID-19) study reported a higher blood glucose level in patients receiving methylprednisolone. Objectives This study aims to analyze the association between corticosteroids and COVID-19–related outcomes in patients admitted to the medical ICU (MICU) for COVID-19 pneumonia. Methods This is an observational study of 141 patients admitted to the MICU between March 18 and June 7, 2020. Data on demographics, laboratory and imaging studies, and clinical course were obtained, including data on corticosteroid use. Bivariate analyses and logistic regression were performed between patient characteristics and mortality and successful extubation. Results Of the 141 patients, 86 required mechanical ventilation, 50 received steroids, and 71 died. Regarding demographics, patients had a median age of 58 (interquartile range [IQR] 48, 65), Hispanic (57.4%, n=81), and non-Hispanic Black (37.5%, n=53). The most prevalent comorbidities were hypertension (49.6%, n=70) and diabetes (48.2%, n=68). Lower blood glucose levels on admission (125.5 vs. 148 mg/dL, p=0.025) and lower peak blood glucose levels on corticosteroids (215.5 vs. 361 mg/dL, p=0.0021) were associated with lower prevalence of mortality. Patients who were successfully extubated had a lower admission blood glucose (126.5 vs. 149 mg/dL, p=0.0074) and lower peak blood glucose on corticosteroids (217 vs. 361 mg/dL, p=0.0023). Conclusions Lower blood glucose on admission and lower maximum blood glucose on corticosteroids were associated with lower odds of mortality and successful extubation, regardless of preexisting diabetes. Hyperglycemia may be negating any potential benefit of corticosteroid therapy. These findings suggest that glucose control could be a parameter that impacts the outcome of patients receiving corticosteroids for COVID-19 pneumonia.

scholarly journals ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSA

2013 ◽  
Vol 13 (2) ◽  
pp. 116 ◽  
Author(s):  
Nafila Salma ◽  
Jessy Paendong ◽  
Lidya I Momuat ◽  
Sariyana Togubu
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Blood Sugar ◽  
Plant Extract ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats

ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSAABSTRAK Telah dilakukan penelitian untuk menentukan dosis optimum dari ekstrak tumbuhan suruhan (Peperomia pellucida [L.] Kunth) sebagai antihiperglikemik tikus wistar yang diinduksi sukrosa. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan tikus putih jantan wistar berjumlah 20 ekor tikus yang dibagi dalam 5 kelompok perlakuan yaitu kelompok perlakuan kontrol negatif (K-) diberi CMC 0,5%, kelompok perlakuan kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok perlakuan ekstrak tumbuhan suruhan  dosis 20; 40; 80 mg/kgBB. Data diperoleh dari pemeriksaan kadar gula darah puasa (basal), 30 menit setelah diindukasi sukrosa dan pada menit ke 30, 60, dan 120 setelah diinduksi sediaan per oral. Data diolah dengan uji ANOVA dan Ducan untuk membandingkan antara kelompok kontrol negatif, ekstrak dan kontrol positif. Data hasil pengujian kadar glukosa darah dengan dosis 20; 40; 80 mg/kgBB memberikan penurunan kadar glukosa darah. Pemberian ekstrak tumbuhan suruhan dengan dosis 40 mg/kgBB pada menit ke-120 memberikan hasil  yang signifikan dan bebeda nyata dibandingkan kelompok kontrol negatif (CMC 0,5%) dan tidak berbeda nyata dengan glibenklamid dosis 0,45 mg/kgBB. Kesimpulannya pemberian ekstrak tumbuhan suruhan dosis 40 mg/kgBB pada menit ke 120  memberikan penurunan kadar glukosa darah yang efektif dan berbeda nyata (signifikan) dibandingkan pemberian ekstrak tumbuhan suruhan dosis 20 dan 80 mg/kgBB. Kata Kunci : Peperomia pellucida [L.] Kunth, Tumbuhan Suruhan, Kadar Glukosa Darah, Sukrosa. ANTIHYPERGLYCEMIC FROM SURUHAN PLANT (Peperomia pellucida [L.] Kunth) EXTRACT AGAINST WISTAR RATS (Rattus norvegicus L.) WITH INCLUDED BY SUCROSE ABSTRACT The purpose of this research is to determine optimum dose of Suruhan (Peperomia pellucida [L.] Kunth) extract as Antihyperglycemic agent against Wistar Rats with Induced by Sucrose. This research using glucose tolerance test methods. Used 20 male Wistar rats were divided into 5 groups, they are the negative control treatment group (K-) were given 0.5% CMC, the positive control groups (K+) given 0,45 mg/kgBB  Glibenklamid, and Suruhan extract treatment group of 20; 40; 80 mg/kgBB dose. Data obtained from the examination offasting blood sugar (basal) content, 30 minutes after induced bysucroseandat minute of 30, 60, and120 after-induced oraldosage.The data were processed by ANAVA and LSD testtocompare betweenthe negativecontrol group, the extractandpositive control. Dataresults of testing blood sugar levels with doses of 20, 40, 80 mg/kgBB gavea decreasein blood glucose levels were significant and significantly different than the negative control group (CMC 0.5%) and did not give a clear difference with 0.45 mg/kgBB dose of Glibenclamide. Agents of plant extract at a dose of 40 mg/kgBB body weight gives better results to the decline in blood glucose levels compared with doses of 20 and 80 mg/kgBB. In conclusion, Suruhan plant extract has an effect on blood glucose levels decreased against white male Wistar rats were induced sucrose. Keywords : Peperomia pellucida [L.] Kunth, Suruhan Plant, Blood Glucose Levels, Sucrose

scholarly journals Activity Antidiabetic Of The Ethanol Extract Green Gedi Leaves Medical) On Mice (Mus musculus

MEDULA ◽  
2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Parawansah Parawansah
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Effective Concentration ◽  
Lower Blood Glucose ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lower Blood

ABSTRACTBackground: Green  gedi leaves  (Abelmoschus manihot (L.)  Medik)  is one plant that has efficacy in treating various diseases one of which is antidiabetic. The nature of the active substance green gedi leaves that capable lower blood glucose levels are flavonoids. Purpose: The purpose of this  study  was  to  determine  the effective  concentration  of  the  extract  of  green  gedi  leaves (Abelmoschus  manihot  (L.)  Medik)  to  lower  the  blood  glucose  levels on  mice  Induced  by Streptozotocin. Method: This type of research is experimental research that consists of 5 treatments with 3 variations dose and 2 controls. The study was conducted by measuring the blo od glucose levels in mice, blood glucose levels after induction to return normal blood glucose levels after oral administration with a suspension of Na. CMC as a control, and the suspension glibenclamid as a comparison with the extract of green gedi leaves concentration of 30 mg/KgBB, 60 mg/KgBB and 90 mg/KgBB with a time of observation for seven days. Result: The data obtained were analyzed using ANOVA and LSD. Conclusion: From this study that the green gedi leaf extract (Abelmoschus manihot (L.) Medik) at a concentration of 90 mg/kgBB is more effectife to give antidiabetic effect with the average reduction in blood glucose levels reach 68,67 mg/dL to mice.Keywords: antidiabetic, abelmoschus manihot (L.) Medik, green gedi leaves, Streptozotocin

scholarly journals Dapagliflozin suppresses glucagon signaling in rodent models of diabetes

2017 ◽  
Vol 114 (25) ◽  
pp. 6611-6616 ◽  
Author(s):  
May-yun Wang ◽  
Xinxin Yu ◽  
Young Lee ◽  
Sara Kay McCorkle ◽  
Shiuhwei Chen ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucagon Secretion ◽  
Zucker Rats ◽  
Hepatic Glycogen ◽  
Alpha Cells ◽  
Lower Blood Glucose ◽  
Glucagon Receptor ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lower Blood

Sodium-glucose cotransporter 2 (SGLT2) inhibitors are a class of antidiabetic drug used for the treatment of diabetes. These drugs are thought to lower blood glucose by blocking reabsorption of glucose by SGLT2 in the proximal convoluted tubules of the kidney. To investigate the effect of inhibiting SGLT2 on pancreatic hormones, we treated perfused pancreata from rats with chemically induced diabetes with dapagliflozin and measured the response of glucagon secretion by alpha cells in response to elevated glucose. In these type 1 diabetic rats, glucose stimulated glucagon secretion by alpha cells; this was prevented by dapagliflozin. Two models of type 2 diabetes, severely diabetic Zucker rats and db/db mice fed dapagliflozin, showed significant improvement of blood glucose levels and glucose disposal, with reduced evidence of glucagon signaling in the liver, as exemplified by reduced phosphorylation of hepatic cAMP-responsive element binding protein, reduced expression of phosphoenolpyruvate carboxykinase 2, increased hepatic glycogen, and reduced hepatic glucose production. Plasma glucagon levels did not change significantly. However, dapagliflozin treatment reduced the expression of the liver glucagon receptor. Dapagliflozin in rodents appears to lower blood glucose levels in part by suppressing hepatic glucagon signaling through down-regulation of the hepatic glucagon receptor.

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