scholarly journals STUDY OF SOYBEAN SEED MICROFLORA AS А ONE OF PROBABLE FACTORS OF BRADYRHIZOBIUM JAPONICUM DISTRIBUTION

2008 ◽  
Vol 6 ◽  
pp. 84-91
Author(s):  
D.V. Krutуlo
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Soybean Seed ◽  
Seed Storage ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Soybean Rhizobia ◽  
Soybean Nodule ◽  
Deep Layers

The results of studying of the soybean nodule bacteria presence in epiphytic and endophytic microflora of different grades of soybean seeds are presented. The soybean rhizobia was not revealed on the surface and in deep layers of soybean seeds. The soybean seed microbiota of investigated varieties is presented by several morphological types of bacteria and fungy. Their quantity depends on the period of seed storage.

scholarly journals Response of Soybean to Seed Inoculation with Bradyrhizobium japonicum and with Mixed Inoculants of B. japonicum and Azotobacter chroococcum

2013 ◽  
Vol 62 (4) ◽  
pp. 457-460 ◽  
Author(s):  
MONIKA KOZIEŁ ◽  
BARBARA GĘBALA ◽  
STEFAN MARTYNIUK
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Effective Strain ◽  
Soybean Seed ◽  
Azotobacter Chroococcum ◽  
Soybean Seeds ◽  
Seed Inoculation ◽  
Soybean Rhizobia ◽  
Pure Cultures ◽  
Soybean Plants ◽  
Seed Yields

Effects of pre-sowing soybean seed inoculation with Bradyrhizobium japonicum alone or with mixed inoculants containing soybean rhizobia and Azotobacter chroococcum were compared. In the pot experiment all the tested strains of soybean rhizobia in pure cultures or in mixtures with A. chroococcum significantly improved nodulation of soybean plants and seed yields of this crop. In micro-plot experiments pre-sowing soybean seeds treatment with the inoculant containing the most effective strain 94P of B. japonicum alone or with the mixed inoculant of strain 94P and A. chroococcum were equally effective in improving nodulation intensity and seed yields of soybean in comparison to the uninoculated soybean.

scholarly journals THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AT CULTIVATION IN THE GEL MEDIUM

2011 ◽  
Vol 13 ◽  
pp. 108-116
Author(s):  
I.O. Kameneva
Keyword(s):  
Liquid Medium ◽  
Medium Composition ◽  
Nodule Bacteria ◽  
Cultivation Conditions ◽  
Rhizobium Strains ◽  
Soybean Rhizobia ◽  
Soybean Nodule ◽  
Growth Activity ◽  
Maximum Titer ◽  
Gel Preparation

The influence of exopolysaccharideacrylamide (EPAA) in a liquid medium on the growth, retaining of viability and virulence of soybean rhizobia under the periodic cultivation conditions was investigated. It was observed that maximum titer of B. japonicum M-8 (B-7198) and B. japonicum 36 (B-1798) at thickening of medium has increased by 31.4% and 79.4% respectively. In the phase of exponential growth the upward trend in growth activity of rhizobia in the gel medium was observed. It was shown that the B. japonicum 36 is more sensitive than B. japonicum M-8 to the changes in the medium composition and the cultivation conditions. Absolute speed of B. japonicum 36 growth has increased in 5 times while the generation time has decreased in almost 3 times in comparison to the liquid medium. The viability and virulence of both rhizobium strains have remained over five months. It was established that the EPAA is a technological agent for the medium thickening and stabilization of the created on the basis of the soybean nodule bacteria gel preparation.

scholarly journals THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE IN THE MIXED CULTURE IN VITRO

2011 ◽  
Vol 13 ◽  
pp. 70-81
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova ◽  
T.A. Zherebor
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Stress Factors ◽  
Lag Phase ◽  
Bacterial Cells ◽  
Nodule Bacteria ◽  
Initial Concentration ◽  
Soybean Nodule ◽  
Growth Activity

As a result of the conducted researches the substantial influence of azospirillium bacteria on the growth activity of nodule bacteria as comparing to the influence of Bradyrhizobium japonicum on growth of Azospirillum brasilense was found out. The greatest quantity of the studied bacteria was observed at average initial concentration (0,272 milliard/cm3) of bacterial cells of every kind at their initial ratio 1:1. It was shown that combined cultivation of soybean nodule bacteria and azospirillium bacteria reduces effect of stress factors on the growth of B. japonicum in the lag-phase.

scholarly journals THE EFFICIENCy OF DIFFERENT MEANS OF SOyBEAN INOCULATION wITH NODULE BACTERIA

2010 ◽  
Vol 10 ◽  
pp. 20-28
Author(s):  
S.M. Malichenko ◽  
V.K. Datsenko ◽  
P.M. Mamenko ◽  
S.Ya. Kots
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Standard Strain ◽  
Bacterial Cultures ◽  
Tn5 Mutants ◽  
Soybean Plants ◽  
Production Strain

The efficiency of the presowing soybean inoculation with nodule bacteria at direct seeds bacterization or introduction of inoculum to the soil at presowing cultivation as well as the ability of rhizobia remained in the soil to the next year to form active symbioses with soybean plants was studied. The liquid bacterial cultures of production strain Bradyrhizobium japonicum 634b and three perspective Tn5-mutants of B. japonicum 646 were used. The introduction of the inoculum to soil was shown to be more efficient during both years of investigations as compared with the seeds inoculation which resulted in higher number of nodules formed, their nitrogenase activity and greater soybean seeds yield. Two of three studied Tn5-mutants had surpassed the standard strain by the efficiency indices.

Вплив глюкозовмісних моноцукрів на симбіотичнi властивостi бульбочкових бактерій сої та формування урожаю рослинами

2018 ◽  
Vol 8 (1) ◽  
pp. 460-465 ◽  
Author(s):  
O.V. Kyrychenko ◽  
Yu.O. Khomenko ◽  
S.Ya. Kots
Keyword(s):  
High Efficiency ◽  
Nitrogenase Activity ◽  
Root Nodules ◽  
Control Level ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Natural Agents ◽  
Soybean Nodule ◽  
Productive Potential ◽  
Symbiotic Properties

<p><span lang="EN-US">The symbiotic properties of soybean nodule bacteria, root nodules forming (nodulation ability), nitrogen-fixing activity and efficiency under the influence of monosaccharides (0.01 M glucose and N-acetyl-D-glucosamine) on rhizobium culture were investigated in greenhouse with sandy soils. Nodulation activity and efficiency was estimated by the number and weight of the nodules formed on the roots of plants; by the formation of vegetative mass and by the yield of soybean seeds. Nitrogenase activity was determined by acetylene-reductase method. The control variant was inoculated by monoculture of soybean rhizobium. A significant increase in the degree of realization of the nodulation ability of rhizobium has been established under the influence of glucose-containing monosaccharides on the culture of microorganisms. Adding glucose to bacteria led to increase the average number of nodules per plant by 1.6, 2.2, and 1.7 times respectively in the phase of development of two true leaves, flowering and active bean formation in soybean. At the same time the weight of the root nodules was increased by 1.4, 2.3, and 1.4 times respectively as compared to control. The number of root nodules was increased by 2.2, 2.3, and 1.4 times as compared to the control while of glucosamine was used; while the </span><span lang="EN-US">weight of these nodules were 2.1 and 1.9 times higher than control in the phase of development of two true leaves and flowering, while in the phase of active beans formation it did not differ from control.</span><span lang="EN-US">T</span><span lang="UK">he functional activity</span><span lang="UK">of the soybean symbiotic </span><span lang="EN-US">system that was formed by</span><span lang="UK"> rhizobial culture </span><span lang="EN-US">and modified by </span><span lang="UK">glucose</span><span lang="EN-US">, had </span><span lang="UK">the highest positive effect, since the nitrogen</span><span lang="EN-US">-fixing</span><span lang="UK"> activity remained stable and </span><span lang="EN-US">was </span><span lang="UK">2.1 and 1.7 times</span><span lang="EN-US"> higher than control.</span><span lang="EN-US">Rhizobia, to suspension of which we added glucosamine, formed a symbiosis with activity that was 1.7 times higher than monoculture in the flowering phase, but later it was at the control level. We registered that inoculants on the basis of bacteria and glucose-containing monosaccharides activated seeds germination. The first true leaf of plants (up to 3.5 times higher than control), their above green mass (25-27% higher) and root system (10-16% higher) were actively formed while we used inoculant with rhizobia and glucose. Plants in the variant with the pre-sowing inoculation of seeds by bacteria and glucosamine almost did not differ from the control.</span><span lang="EN-US">The yield of soybean seeds significantly exceeded (up to 14%) the crop, produced by plants with inoculation by monoculture of rhizobia under the influence of glucose-containing monosaccharides. We indicated high efficiency of soybean-rhizobium symbiosis formed by nodule bacteria modified of glucose-containing monosaccharides. </span><span lang="UK">Thus, the use of glucose-containing mono</span><span lang="EN-US">saccherides </span><span lang="UK">as additional </span><span lang="EN-US">“green” and </span><span lang="UK">safe natural agents in complex inoculants with </span><span lang="EN-US">nodule </span><span lang="UK">soybean bacteria promotes a more complete implementation of the symbiotic and productive potential of soybean-rhizobial symbiosis compared with the use for seed</span><span lang="EN-US">s</span><span lang="UK"> pre-sowing treatment </span><span lang="EN-US">only </span><span lang="UK">of rhizobia monoculture in </span><span lang="EN-US">greenhouse </span><span lang="UK">with sandy </span><span lang="EN-US">culture</span><span lang="EN-US">.</span></p>

scholarly journals VIABILITY AND EFFICIENCY OF BRADYRHIZOBIUM JAPONICUM UNDER VARIOUS TECHNOLOGIES OF BACTERIAL PREPARATION PRODUCTION

2007 ◽  
Vol 4 ◽  
pp. 39-50
Author(s):  
S.Ya. Kots ◽  
D.M. Sytnikov ◽  
S.M. Malichenko ◽  
N.A. Vorobey
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Bacteria ◽  
Bacterial Preparation ◽  
Soybean Nodule ◽  
Bacterial Preparations

Viability of soybean nodule bacteria under various technologies of production of bacterial preparations and influence of homologous lectin on their efficiency had been studied. It is shown that perlite and vermiculite are equivalent carriers in terms of technology for product¬ion of bacterial preparations. The findings indicate perspectiveness of homologous lectin ме as a component of bacterial preparations.

scholarly journals THE INFLUENCE OF ACTIVE STRAINS OF NODULE BACTERIA ON EFFICIENCY OF LEGUMINOUS PLANTS

2007 ◽  
Vol 4 ◽  
pp. 51-61
Author(s):  
E.V. Nadkernichna ◽  
T.M. Kovalevska ◽  
D.V. Krutilo ◽  
V.P. Gorban ◽  
V.S. Vorobej
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Rhizobium Leguminosarum ◽  
Nodule Bacteria ◽  
Leguminous Plants ◽  
Soybean Nodule ◽  
Bradyrhizobium Sp ◽  
Bacterial Preparations

The results of efficiency studying of new active strains of lupine, peas and soybean nodule bacteria in field and manufacture experiments are given. It is shown, that strains Bradyrhizobium sp. (Lupinus) 5500/4, Rhizobium leguminosarum bv.viceae T2, Bradyrhizobium japonicum КНЮ provide stable increase of leguminous culture crop in comparison with standard strains and they can be used to bacterial preparations manufacture.

scholarly journals Determination of toxicity of various preparative forms of pesticidal fungicides for nodule bacteria inoculants

2020 ◽  
Vol 224 ◽  
pp. 04032
Author(s):  
Y V Laktionov ◽  
Y V Kosulnikov ◽  
V V Yachno ◽  
A P Kozhemyakov
Keyword(s):  
Temperature Regime ◽  
Active Substance ◽  
Bradyrhizobium Japonicum ◽  
Rhizobium Leguminosarum ◽  
Holding Time ◽  
Expert Group ◽  
Nodule Bacteria ◽  
Biological Products ◽  
Soybean Nodule

The aim of our study was to determine the effect of fungicide formulation, brand of fungicide, its concentration in the solution, holding time and temperature regime of the solution on the number of survivors of nodule bacteria of soybean, lupine, peas and lentils in a solution. Bacterial suspensions of soybean nodule bacteria (Bradyrhizobium japonicum 634b), lupine (Bradyrhizobium lupini 367a), pea (Rhizobium leguminosarum 261b), and lentils (Rhizobium leguminosarum 712) were studied. Wetting powders Benomil (active substance benomil 500 g/kg, LLC “Soyuzagrohim”, Russia), Benorad (active substance benomil 500 g/kg, JSC “August”, Russia) and Fundazol (active substance benomil 500 g/l, LLC “Agro-Kemi”, Russia) and concentrates of suspension Maxim KS (active substance fludioxonil, 25 g/l; “Syngenta International AG”, Switzerland), Protect KS (active substance fludioxonil, 25 g/l; LLC “Agro Expert Group”, Russia), Protect Forte VSK (active substance fludioxonil, 40 g/l + flutriafol, 30 g/l; LLC “Agro Expert Group”, Russia) were studied as fungicidal disinfectants. Compatibility was determined after the preparation of tank solutions of biological products and fungicides, followed by an assessment of the percentage of surviving rhizobia depending on the brand of fungicide, its concentration (10 and 20%), the holding time of the solution (2, 4, 8 h) and the temperature regime of the solution (2-5, 16-18, 27 °C).

scholarly journals EFFICACY OF SOYBEAN SEEDS SORPTION DRYING AND PREHARVEST TREATMENT WITH DESICCANTS AS A COMPREHENSIVE APPROACH

2020 ◽  
Vol 17 (36) ◽  
pp. 119-127
Author(s):  
Mira SERIKKYZY ◽  
Aleksei AVDEENKO ◽  
Yoro Ella GOULY ◽  
Igor TYURIN ◽  
Diana STEPANOVA
Keyword(s):  
Moisture Content ◽  
Soybean Seed ◽  
Seed Storage ◽  
Control Site ◽  
Soybean Seeds ◽  
Subsequent Increase ◽  
Physiological Processes ◽  
Activity Of Enzymes ◽  
Soybean Plants ◽  
Study Sites

The purpose of the study was to evaluate the efficacy of soybean seed treatment with desiccants and through post-harvest drying. A total of 120 quadruplicate study sites were divided into two groups of 60, one of which was not sprayed with desiccants (control sites), and the other received desiccant treatment (experimental sites). A week after the beginning of the experiment, seeds from experimental sites had 1.5 times less moisture content than seeds from control sites (p ≤ 0.05). The peak lipase activity of seeds was recorded during the first 50 days of storage. The lipoxygenase activity was found to increase between 50 and 100 days of seed storage. The activity of lipase in seeds increased significantly at 60 °C (p ≤ 0.05), but with the subsequent increase in temperature to 80 and 100 °C, a noticeable reduction was recorded (p ≤ 0.01). For lipoxygenase, a similar decrease in activity reaction was noticed at temperatures of drying agents starting from 80 °C. For other factors, no similar results were recorded. This means that the activity of enzymes, in particular, lipase and lipoxygenase, plays a key role in the physiological processes of soybean seeds during storage, but can be inhibited at temperatures starting from 80 °C. Desiccants application accelerates the maturation of the seed to the necessary moisture content for two weeks. After seven days of treatment with Reglon, the yellowing of beans and leaves in the lower part of soybean plants was noted as the moisture content of leaves and beans decreased almost twice to 35%, while on the control site, these values were 48% (p ≤ 0.05).

scholarly journals THE EFFECT OF STORAGE METHODS ON VIABILITY AND SYMBIOTIC PROPERTIES OF SOYBEAN NODULE BACTERIA

2014 ◽  
Vol 20 ◽  
pp. 15-22
Author(s):  
T. M. Kovalevska ◽  
D. V. Krutylo ◽  
V. P. Horban ◽  
I. M. Romanova ◽  
T. O. Usmanova
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Bacteria ◽  
Partial Restoration ◽  
Long Term Storage ◽  
Soybean Nodule ◽  
Storage Methods ◽  
Soybean Plants ◽  
Symbiotic Properties ◽  
Term Storage

The viability and symbiotic properties of active strain Bradyrhizobium japonicum 46 after its long-term storage using different methods were studied. It was established that strain storing in evaporative freezing conditions as well at periodic passages the strain preserves its ability to form active symbiosis with soybean plants. Long-term storage under the layer of mineral oil had negatively influenced on the symbiotic properties of B. japonicum 46 strain resulted in only partial restoration of strains virulence, nitrogen fixing activity and competitiveness.

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