scholarly journals Response of Soybean to Seed Inoculation with Bradyrhizobium japonicum and with Mixed Inoculants of B. japonicum and Azotobacter chroococcum

2013 ◽  
Vol 62 (4) ◽  
pp. 457-460 ◽  
Author(s):  
MONIKA KOZIEŁ ◽  
BARBARA GĘBALA ◽  
STEFAN MARTYNIUK
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Effective Strain ◽  
Soybean Seed ◽  
Azotobacter Chroococcum ◽  
Soybean Seeds ◽  
Seed Inoculation ◽  
Soybean Rhizobia ◽  
Pure Cultures ◽  
Soybean Plants ◽  
Seed Yields

Effects of pre-sowing soybean seed inoculation with Bradyrhizobium japonicum alone or with mixed inoculants containing soybean rhizobia and Azotobacter chroococcum were compared. In the pot experiment all the tested strains of soybean rhizobia in pure cultures or in mixtures with A. chroococcum significantly improved nodulation of soybean plants and seed yields of this crop. In micro-plot experiments pre-sowing soybean seeds treatment with the inoculant containing the most effective strain 94P of B. japonicum alone or with the mixed inoculant of strain 94P and A. chroococcum were equally effective in improving nodulation intensity and seed yields of soybean in comparison to the uninoculated soybean.

scholarly journals STUDY OF SOYBEAN SEED MICROFLORA AS А ONE OF PROBABLE FACTORS OF BRADYRHIZOBIUM JAPONICUM DISTRIBUTION

2008 ◽  
Vol 6 ◽  
pp. 84-91
Author(s):  
D.V. Krutуlo
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Soybean Seed ◽  
Seed Storage ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Soybean Rhizobia ◽  
Soybean Nodule ◽  
Deep Layers

The results of studying of the soybean nodule bacteria presence in epiphytic and endophytic microflora of different grades of soybean seeds are presented. The soybean rhizobia was not revealed on the surface and in deep layers of soybean seeds. The soybean seed microbiota of investigated varieties is presented by several morphological types of bacteria and fungy. Their quantity depends on the period of seed storage.

scholarly journals Adhesion of uredospores of Phakopsora pachyrhizi on soybean seeds and their viability during storage

2012 ◽  
Vol 34 (2) ◽  
pp. 225-230
Author(s):  
Elisandra Batista Zambenedetti Magnani ◽  
Elisabeth Aparecida Furtado de Mendonça ◽  
Maria Cristina de Figueiredo e Albuquerque
Keyword(s):  
Soybean Seed ◽  
Germination Percentage ◽  
Time Span ◽  
Soybean Seeds ◽  
Phakopsora Pachyrhizi ◽  
Mato Grosso ◽  
Soybean Production ◽  
Soybean Plants ◽  
Production Areas

To study adhesion and viability of uredospores of the fungus Phakopsora pachyrhizi on soybean seeds during storage, suspension tests of those uredospores were carried out by washing seeds at each 30 days interval. Furthermore, germination and inoculation tests of uredospores on soybean plants were performed with uredospores collected from seeds of two soybean production areas, located in the municipalities "Chapada dos Guimarães" and "Tangará da Serra", State of Mato Grosso, Brazil. High levels of uredospores infestation were detected before storage [249.31 and 85.18 uredospores/100 seeds (U/100)] on seeds collected in both localities, respectively. After 30 days storage, these figures were 46.12 and 122.5 U/100; at 60 days were 14.62 and 26.62 U/100; and at 90 days were only 2.87 and 3,68 U/100, respectively; dropping to zero after 120 days storage. The percentage of germinated uredospores decreased with increasing storage periods and at 120 days germination percentage was nil. When uredospores were inoculated on soybean plants, rust symptoms were only observed for uredospores collected from freshly harvested seeds. Uredospores associated to soybean seed germinate until 90 days after storage, but are not viable after this time span. Infection of plants only occurs with inoculation of uredospores obtained from freshly harvested seeds.

Dicamba translocation in soybean and accumulation in seed

Weed Science ◽  
2020 ◽  
Vol 68 (4) ◽  
pp. 333-339
Author(s):  
Maria Leticia M. Zaccaro ◽  
Jason K. Norsworthy ◽  
Chad B. Brabham
Keyword(s):  
Seed Quality ◽  
Soybean Seed ◽  
Cropping System ◽  
Soybean Seeds ◽  
Amaranthus Palmeri ◽  
Weed Species ◽  
Target Movement ◽  
Trifoliate Leaf ◽  
Plant Parts ◽  
Soybean Plants

AbstractThe dicamba-resistant cropping system was developed to be used as a tool to control multiple-resistant weed species, particularly Palmer amaranth (Amaranthus palmeri S. Watson). However, dicamba applications have resulted in off-target movement of the herbicide to susceptible neighboring vegetation, with frequent damage to non–dicamba resistant soybean [Glycine max (L.) Merr.]. Pod malformation and subsequent auxin-like injury to progeny is common when parent soybean plants are exposed to the herbicide post-flowering. Yet no publication to date has conveyed the presence of dicamba in seed. The objective of this study was to determine whether dicamba exists and at what quantities inside soybean seed following a low-dose exposure in the pod-filling stage using radiolabeled herbicide as a tracer. Non–dicamba resistant soybean plants were grown in the greenhouse until the pod-filling growth stage and then treated with 2.8 g ae ha−1 of dicamba (1/200 of the recommended rate of 560 g ae ha−1). Immediately afterward, [14C]dicamba (approximately 6.4 kBq per plant) was applied to the adaxial surface of one trifoliate leaf located in the midportion of each plant. The greatest amount of [14C]dicamba recovered was in seeds and in pods, and these plant parts accumulated 44% and 38% of the total absorbed, respectively. Chromatography results showed that the totality of the [14C]dicamba present in the soybean seeds was in the phytotoxic form, except for a single sample, in which one metabolite was detected (possibly 5-hydroxy dicamba). Precautions should be taken to avoid dicamba exposure to sensitive soybean fields, especially those dedicated to seed production, as this may result in low seed quality and symptomology on progeny plants.

Effect of numbers of Bradyrhizobium japonicum applied in commercial inoculants on soybean seed yield in Ontario

1992 ◽  
Vol 38 (6) ◽  
pp. 588-593 ◽  
Author(s):  
D. J. Hume ◽  
D. H. Blair
Keyword(s):  
Glycine Max ◽  
Seed Yield ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Soybean Seed ◽  
Yield Response ◽  
Cubic Equation ◽  
Glycine Max L ◽  
Seed Yields ◽  
Commercial Inoculants

In the absence of Bradyrhizobium japonicum populations in the soil, yields of field-grown soybean (Glycine max (L.) Merrill) usually respond to inoculation with B. japonicum. The objective of this research was to determine the relationship between numbers of B. japonicum per seed in inoculants and soybean nodulation and yield. A total of six field experiments were conducted in 1989 and 1990 on new soybean soils. In dilution trials, Grip inoculant was applied to provide approximately 106, 105, 104, and 103B. japonicum per seed at two locations in 1989. Nodule number and mass, as well as seed yield, increased curvilinearly upward with increasing log10 most probable numbers (MPNs) of B. japonicum. The yield response curve was best fit by a cubic equation, which accounted for 97% of the variation in yield. Seed yields increased 19% (1.83 to 2.13 Mg/ha) from 105 to 106B. japonicum per seed. In field experiments involving 8 commercial inoculants in 1989 and 10 in 1990, and conducted at two locations in each year, responses to increasing log MPNs in the inoculants also were concave upwards and cubic. In the two years, 78 and 46% of the yield variation was accounted for by log MPN per seed. Increasing MPN per seed from 105 to 106 improved yields in first-time fields by an average of 24%, indicating the present minimum standard of 105B. japonicum per seed should be increased. Key words: most probable numbers, response to inoculation, nodulation, Glycine max (L.) Merrill.

scholarly journals Survival and Competitiveness of Bradyrhizobium japonicum Strains 20 Years after Introduction into Field Locations in Poland

2015 ◽  
Vol 81 (16) ◽  
pp. 5552-5559 ◽  
Author(s):  
Dorota Narożna ◽  
Krzysztof Pudełko ◽  
Joanna Króliczak ◽  
Barbara Golińska ◽  
Masayuki Sugawara ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Indigenous Populations ◽  
Content Type ◽  
Soybean Rhizobia ◽  
Competitive Relations ◽  
Competitive Success ◽  
Soybean Plants ◽  
Soybean Nodulation

ABSTRACTIt was previously demonstrated that there are no indigenous strains ofBradyrhizobium japonicumforming nitrogen-fixing root nodule symbioses with soybean plants in arable field soils in Poland. However, bacteria currently classified within this species are present (together withBradyrhizobium canariense) as indigenous populations of strains specific for nodulation of legumes in the Genisteae tribe. These rhizobia, infecting legumes such as lupins, are well established in Polish soils. The studies described here were based on soybean nodulation field experiments, established at the Poznań University of Life Sciences Experiment Station in Gorzyń, Poland, and initiated in the spring of 1994. Long-term research was then conducted in order to study the relation betweenB. japonicumUSDA 110 and USDA 123, introduced together into the same location, where no soybean rhizobia were earlier detected, and nodulation and competitive success were followed over time. Here we report the extra-long-term saprophytic survival ofB. japonicumstrains nodulating soybeans that were introduced as inoculants 20 years earlier and where soybeans were not grown for the next 17 years. The strains remained viable and symbiotically competent, and molecular and immunochemical methods showed that the strains were undistinguishable from the original inoculum strains USDA 110 and USDA 123. We also show that the strains had balanced numbers and their mobility in soil was low. To our knowledge, this is the first report showing the extra-long-term persistence of soybean-nodulating strains introduced into Polish soils and the first analyzing the long-term competitive relations of USDA 110 and USDA 123 after the two strains, neither of which was native, were introduced into the environment almost 2 decades ago.

scholarly journals Root cell growth and division in soybean in the presence of base trichoderma and "Bradyrhizobium japonicum" bioproducts

2020 ◽  
Vol 42 ◽  
pp. e21
Author(s):  
Luana Da Silva Cadore ◽  
Kássia Cauana Trapp ◽  
Rosana Taschetto Vey ◽  
Lucas Dotto ◽  
Solange Bosio Tedesco ◽  
...  
Keyword(s):  
Root Growth ◽  
Mitotic Index ◽  
Bradyrhizobium Japonicum ◽  
Soybean Seed ◽  
Control Treatment ◽  
Initial Development ◽  
Soybean Seedlings ◽  
Seed Inoculation ◽  
Trichoderma Sp ◽  
Randomized Experimental Design

The objective of this work was to evaluate the effect of commercial formulations of biological products on mitotic index and root growth, as well as on the initial development of soybean crop. A soybean cultivar Nidera 5909 was used, composed of four commercial bioformulates that had in its composition Trichoderma harzianum, Trichoderma sp. Bradyrhizobium japonicum. The products used were tested in combination or isolated for soybean seed inoculation in a randomized experimental design. In the variable length of shoot using Trichoderma sp., B. japonicum peat and Trichoderma sp. associated with B. japonicum peat did not differ from the control treatment but differed from the others. There was an increase in root length of soybean seedlings for the treatments T. harzianum + B. japonicum liquid and T. harzianum + B. japonicum peat compared to the control, as well as an increase in the mitotic index for the same treatments. The treatments T. harzianum + B. japonicum liquid and T. harzianum + B. japonicum provided higher root growth of soybean seedlings and higher initial plant growth.

scholarly journals THE EFFICIENCy OF DIFFERENT MEANS OF SOyBEAN INOCULATION wITH NODULE BACTERIA

2010 ◽  
Vol 10 ◽  
pp. 20-28
Author(s):  
S.M. Malichenko ◽  
V.K. Datsenko ◽  
P.M. Mamenko ◽  
S.Ya. Kots
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Standard Strain ◽  
Bacterial Cultures ◽  
Tn5 Mutants ◽  
Soybean Plants ◽  
Production Strain

The efficiency of the presowing soybean inoculation with nodule bacteria at direct seeds bacterization or introduction of inoculum to the soil at presowing cultivation as well as the ability of rhizobia remained in the soil to the next year to form active symbioses with soybean plants was studied. The liquid bacterial cultures of production strain Bradyrhizobium japonicum 634b and three perspective Tn5-mutants of B. japonicum 646 were used. The introduction of the inoculum to soil was shown to be more efficient during both years of investigations as compared with the seeds inoculation which resulted in higher number of nodules formed, their nitrogenase activity and greater soybean seeds yield. Two of three studied Tn5-mutants had surpassed the standard strain by the efficiency indices.

scholarly journals Plant growth analysis describing the soybean plants response on dryland field to seed co-inoculation

2021 ◽  
Vol 51 (9) ◽  
Author(s):  
Pedro Henrique de Medeiros Buso ◽  
Ricardo Augusto de Oliveira ◽  
Edelclaiton Daros ◽  
José Luis Camargo Zambon ◽  
Wilson Story Venancio ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bradyrhizobium Japonicum ◽  
Dry Matter ◽  
Growth Analysis ◽  
Growth Stages ◽  
Seed Inoculation ◽  
Bradyrhizobium Sp ◽  
Soybean Plants ◽  
Vegetative And Reproductive Growth ◽  
Microbial Effects

ABSTRACT: Plant growth analysis can be used for soybean plants evaluation to identify morphologic changes caused by soil microbes after seed inoculation. The objective was to measure changes of inoculated soybean plants grown under regular field Brazilian production conditions. The experiment was carried out to compare 5 inoculation treatments: T1 (Bradyrhizobium japonicum and B. diazoefficiens), T2 (T1 and Azospirillum brasilense), T3 (T1 and Trichoderma asperellum), T4 (T1 and T. virens, and Bacillus amyloliquefaciens) and T5 (T1 and Penicillum bilaiae). Leaf area, shoot and root dry matter were measured at vegetative and reproductive growth stages. Results of this study validate the contribution of plant growth analysis of soybeans plants for identifying their responses promoted by the combined inoculation of their seeds with selected microbes. Also, the microbial effects on plant growth vary not only between microbes but also between parts of the plants and through the plant development stages. The introduction of different microbes in soybean rhizosphere combined with Bradyrhizobium sp. strains can contribute to increase crop dry matter productivity during its growing cycle.

scholarly journals Osmoprotectant in soybean seeds can increase the inoculation and co-inoculation time in pre-sowing

2020 ◽  
pp. 905-912
Author(s):  
Glauber Monçon Fipke ◽  
Thomas Newton Martin ◽  
Tânia Maria Müller ◽  
Vinícius dos Santos Cunha ◽  
Janete Denardi Munareto ◽  
...  
Keyword(s):  
Grain Yield ◽  
Soybean Seed ◽  
First Year ◽  
Soybean Seeds ◽  
Clay Loam ◽  
Total Chlorophyll Content ◽  
Leaf Chlorophyll ◽  
Seed Inoculation ◽  
Inoculation Treatment ◽  
Santa Maria

The practice of soybean seed inoculation cannot exceed the 24-hour prior to sowing. The objective of this study was to assess the effects of inoculation and co-inoculation of osmoprotectant soybean seeds, performed at different times of sowing. The experiments were conducted during two crop seasons at "sandy clay loam Acrisol" and "Cfa climate" in Santa Maria, RS, Brazil. The experimental design consisted of completely randomized blocks with four replications. Nine distinct treatments of seeds inoculation were carried out with bacteria of the genus Bradyrhizobium were inoculated alone (conventional inoculation) or combined with Azospirillum brasilense (co-inoculation), associated with the use of osmoprotectants. The components of nodulation, leaf chlorophyll, plant growth and grain yield were evaluated. The number of nodules and pods per plant are the variables most correlated with grain yield. Number of grains per pod, mass of thousand grains, and A, B and total chlorophyll content were not influenced by inoculation, co-inoculation and nitrogen fertilization. All treatments that used osmoprotectant had an increased 30% (in the first year) at average number of nodules in V5 compared to the treatments that did not use it. Co-inoculation provided an increase of 31% and 16% of yield, respectively, compared to the no-inoculation treatment, for the first and second experimental years. Inoculation and co-inoculation when carried out seven days before sowing and associated with the use of the osmoprotector, provides grain yield similar to the inoculation performed at the time of sowing.

scholarly journals PHYSIOLOGICAL INTERACTION OF SOYBEAN PLANTS AND NEW TN-5 MUTANTS OF BRADYRHIZOBIUM JAPONICUM

2009 ◽  
Vol 8 ◽  
pp. 52-61
Author(s):  
V.K. Datsenko ◽  
V.M. Mel’nyk ◽  
S.Ya. Kots ◽  
S.V. Omel’chuk
Keyword(s):  
Host Plant ◽  
Bradyrhizobium Japonicum ◽  
Direct Relationship ◽  
Root Nodules ◽  
Soybean Seeds ◽  
Nitrogen Fixation Activity ◽  
Soybean Plants ◽  
Symbiotic Properties ◽  
Relationship Of ◽  
Highly Virulent

The influence of soybean seeds inoculation with Tn-5 mutants of Bradyrhizobium japonicum with polar symbiotic properties on symbiosis efficiency, photosynthetic intensity and activity of antioxidant enzymes in root nodules of host plant was studied. Most of the selected mutants were highly virulent, but as was shown there were no considerable correlation between studied parameters. The direct relationship of nitrogen fixation activity of roots nodules and photosynthesis intensity of host plant was established. The two types of dynamics of theses processes were determined with their maximum values in blooming and flowering stages, respectively.  

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