scholarly journals Ultrastructure and biological properties of Avian influenza viruses following cryopreservation

2019 ◽  
Vol 5 (4) ◽  
pp. 18-21
Author(s):  
M. Yu. Stegniy ◽  
B. T. Stegniy
Keyword(s):  
Low Temperature ◽  
Avian Influenza ◽  
Influenza Viruses ◽  
Ultrastructural Changes ◽  
National Endowment ◽  
Avian Influenza Viruses ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage

Ultrastructure and infectious activity of avian influenza virus (strain А/Chicken/Sivash/02/05 (H5N1)) following cryopreservation and low temperature storage at –20, –70, and –196°C during various terms from 25 days up to 143 months using electron microscopy, serological and virological methods were investigated. Avian influenza viruses strain А/Chicken/Sivash/02/05 (H5N1) is stored in the Collection of Pathogens of the National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’ (Kharkiv, Ukraine), which was granted the National Endowment of Ukraine status. The conducted study allowed to reveal on electronograms the ultrastructural changes in AIV during long term storage (18 months) at moderately low temperature (–20°C), in particular loss of glycoprotein of peplomers in the majority of virions. The changes in ultrastructure of the virus samples were accompanied by a loss of hemagglutinating activity during long-term storage of AIV samples at moderately low temperature of –20°C. When storing the AIV samples at –70 and –196°C the virions generally remain negatively contrasted, keep peplomers for the studied storage duration

Long-term Monitoring for Avian Influenza Viruses in Wild Bird Species in Italy

2003 ◽  
Vol 27 ◽  
pp. 107-114 ◽  
Author(s):  
M.A. De Marco ◽  
E. Foni ◽  
L. Campitelli ◽  
E. Raffini ◽  
M. Delogu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Wild Bird ◽  
Bird Species ◽  
Influenza Viruses ◽  
Avian Influenza Viruses ◽  
Long Term Monitoring ◽  
Term Monitoring

Premier Russet: A Dual-Purpose, Potato Cultivar with Significant Resistance to Low Temperature Sweetening During Long-Term Storage

2008 ◽  
Vol 85 (3) ◽  
pp. 198-209 ◽  
Author(s):  
R. G. Novy ◽  
J. L. Whitworth ◽  
J. C. Stark ◽  
S. L. Love ◽  
D. L. Corsini ◽  
...  
Keyword(s):  
Low Temperature ◽  
Potato Cultivar ◽  
Dual Purpose ◽  
Low Temperature Sweetening ◽  
Long Term Storage ◽  
Term Storage

Changes in the content of organic acids and inorganic anions in sugar beet during long-term storage

2016 ◽  
pp. 760-764 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Paulina Miko ◽  
Magdalena Molska ◽  
Ilona Baszczyk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Storage Conditions ◽  
Denitrifying Bacteria ◽  
Raw Material ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Acetic Acids

Due to the prolongation of the period of the sugar campaign, it is necessary to optimize the storage conditions, so that changes in the quality of the raw material could be minimized. The aim of this study was to determine the effect of storage duration and temperature on changes in the composition of sugar beet. The study presents the changes in the content of glucose, fructose, raffinose, lactic and acetic acids, nitrates and nitrites as well as in the content of the total number of mesophilic bacteria, denitrifying bacteria and spores of denitrifying bacteria during storage under various conditions.

Storage duration increases oxidation products in peanut seeds

2021 ◽  
pp. 1-3
Author(s):  
Brandon Tonnis ◽  
Ming Li Wang ◽  
Edward Huang ◽  
Uttam Bhattarai ◽  
Shyam Tallury
Keyword(s):  
Oxidation Products ◽  
Oil Composition ◽  
Storage Duration ◽  
Plant Materials ◽  
Long Term Storage ◽  
Time Out ◽  
Total Oil ◽  
Changes Over Time ◽  
Term Storage

Abstract Seeds kept in long-term storage are essential for maintaining genetic resources of crops and other plant materials in seed banks and national germplasm systems. But seeds undergo chemical changes over time as part of the ageing process that result in reduced germination rates and seedling normalcy. For example, oilseed crops such as peanuts are particularly vulnerable to oxidation and rancidification due to their high oil content. To test the effect of storage time on seed oil in peanuts, we grew different accessions and harvested fresh seeds to compare the oil composition of new seeds with seeds aged in storage for varying lengths of time. Out of the nine fatty acids detected and measured by gas chromatography, five including oleic, gadoleic, behenic, lignoceric and cerotic acids differed significantly between new and old seeds. Additionally, old seeds accumulated up to four oxidation products together averaging about 1% of the total oil composition. The concentration of these oxidation products was positively correlated with the age of the seeds, increasing linearly up to more than 6% of the total in the oldest seeds. The presence and concentration of oxidation products measured using simple chromatography techniques can be used as an initial indication of quality and viability in older seed inventories stored in germplasm repositories.

Effect of long-term storage, ultra-low temperature, and freshness on the quality characteristics of frozen tuna meat

2020 ◽  
Vol 112 ◽  
pp. 270-280 ◽  
Author(s):  
Naho Nakazawa ◽  
Ritsuko Wada ◽  
Hideto Fukushima ◽  
Ryusuke Tanaka ◽  
Shinji Kono ◽  
...  
Keyword(s):  
Low Temperature ◽  
Quality Characteristics ◽  
Long Term Storage ◽  
Term Storage

Low-Temperature Fish Storage Facility with Precise Temperature Control

1969 ◽  
Vol 26 (1) ◽  
pp. 154-161
Author(s):  
K. R. Scott
Keyword(s):  
Steady State ◽  
Low Temperature ◽  
Temperature Control ◽  
Room Temperature ◽  
Storage Facility ◽  
Hot Gas ◽  
Long Term Storage ◽  
Straight Line ◽  
Term Storage

A coldroom complex recently installed at the Fisheries Research Board of Canada, Freshwater Institute laboratory at Winnipeg features several design aspects that are considered novel. These include foamed-in-place urethane insulation, two alternating R-502 refrigeration systems incorporating automatic safety switch-over and adjustable defrost, "straight-line" pneumatic temperature control, hot gas bypass control, and a master panel. The facility combines a cold laboratory at +2 C, a long-term storage room at −37 C, a small anteroom at −26 C, and a room containing eight 10.0-ft3 precise temperature cabinets at −40 C. Room temperature variation is ±0.25 degrees C during steady state conditions. Temperature rise during daily defrosting is less than 2 degrees C for a duration of 1 hr.

Diapause induction and termination in Indian strains of Trichogramma chilonis (Hymenoptera: Trichogrammatidae)

2017 ◽  
Vol 149 (5) ◽  
pp. 607-615 ◽  
Author(s):  
Enakshi Ghosh ◽  
Chandish R. Ballal
Keyword(s):  
Large Scale ◽  
Biocontrol Agent ◽  
Storage Temperature ◽  
Adult Emergence ◽  
Diapause Induction ◽  
Storage Duration ◽  
Trichogramma Chilonis ◽  
Long Term Storage ◽  
Term Storage

AbstractThe role of temperature in diapause induction was studied as a mode of long-term storage of Trichogramma chilonis (Ishii) (Hymenoptera: Trichogrammatidae). Three different strains of this widely used biocontrol agent (T. chilonis Nilgiris strain, T. chilonis Kodaikanal strain, and T. chilonis 15 °C strain) reared on the factitious host Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) were used for this experiment. Except T. chilonis laboratory strain, all the other strains could successfully undergo diapause at their pre-pupal stage. Maximum percentage of healthy pre-pupae were recorded in the three strains by providing a pre-storage temperature of 10 °C for 35 days with eight hours of photophase wherein 75–87% could enter into diapause. Further, at a maintenance temperature of 5 °C with 24 hours of scotophase, diapause could be maintained. Diapause could be terminated after six months of storage with 23–36% of adult emergence. However, there was significant reduction in longevity and parasitism rate of the emerged adults. Considering superior biological parameters, 95 days of storage (including pre-storage duration) could provide around 60% adult emergence. Successful long-term storage of T. chilonis strains through diapause induction can facilitate commercial insectaries in stockpiling this biocontrol agent for large-scale field releases. This is the first study on successful induction and termination of diapause in T. chilonis strains and evaluating their performance attributes.

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