scholarly journals The study of epizootic sera obtained from ruminant animals in complement fixation test (CFT) with the use of Paratuberculous antigen

2019 ◽  
pp. 41-45
Author(s):  
A. I. Zavgorodniy ◽  
S. A. Pozmogova ◽  
N. V. Goncharova ◽  
M. V. Kalashnyk ◽  
V. V. Bilushko
Keyword(s):  
Dairy Cattle ◽  
Culture Filtrate ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Fixation Test ◽  
Laboratory Diagnostics ◽  
Serological Studies ◽  
Methodological Guidelines ◽  
Sera Samples

The paper presents results of the study of epizootic blood sera in the complement fixation test (CFT) with paratuberculous antigen. Blood sera were sampled from the cattle and goats. The antigen was produced from the culture filtrate of Mycobacterium avium subspecies paratuberculosis (MAP) in the laboratory for tuberculosis study. The aim of the present study was to clarify the epizootic situation concerning Johne’s disease among the dairy cattle in different regions of Ukraine. To achieve this aim the blood sera from cattle and goats were collected from farms in different regions of Ukraine. Those sera samples were studied in the complement fixation test with the use of paratuberculous antigen that was produced from the culture filtrate of MAP. The above mentioned blood sera were collected from the cattle that had positive allergic reactions on the use of tuberculin (PPD) for mammals. Those animals belonged to the free from tuberculosis and paratuberulosis milk farms. The study of obtained samples of blood sera was conducted in the accordance with the methodological guidelines “Laboratory diagnostics of paratuberculosis” (shutter. NMR FEFU pr. No. 1, dated December 19, 2014). There were studied 1098 blood sera samples from cattle. In addition to this, investigation was conducted on 24 samples of blood sera from goats. As the result of conducted study it was found that 17 samples of blood sera contained specific antibodies against MAP (serum solution 1:10). These blood sera collected from the cattle belonging to 4 farms in Poltava, Donetsk and Khmelnitsky regions. Along with this it was obtained 9 uncertain results in compliment fixation test that was conducted between paratuberculous antigen (ACF) and blood sera from those 4 farms. The results of monitoring studies indicate that M. avium subsp. paratuberculosis pathogen circulates in studied farms. This can lead to the complication of the epizootic situation regarding paratuberculosis and contribute to the spreading of this pathogen to other free from MAP infection farms. There are no anti-paratuberculosis antibodies in blood serum from goats. It is necessary to conduct annual monitoring serological studies of productive dairy cattle and imported animals in order to clarify and control epizootic situation concerning paratuberculosis on the territory of Ukraine

scholarly journals Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams

2012 ◽  
Vol 47 (No. 7) ◽  
pp. 195-198 ◽  
Author(s):  
M. Trávníček ◽  
D. Kováčová ◽  
Bhide MR ◽  
P. Zubrický ◽  
L. Čisláková
Keyword(s):  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Field Evaluation ◽  
Fixation Test ◽  
Igg Antibodies ◽  
Herd Level ◽  
Chlamydophila Abortus ◽  
Moderate Agreement ◽  
Sera Samples

Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in rams. Indirect ELISA was found to be comparatively more sensitive than CF test in all three groups of animals for detection of IgG antibodies against Chlamydophila abortus. The iELISA used in this study can be used for screening at herd level like CF test, as there is moderate agreement (Kappa – 0.426) between these two tests.

scholarly journals Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test

2021 ◽  
Vol 4 (2) ◽  
pp. 199
Author(s):  
Agung Jati Kusuma ◽  
Erma Safitri ◽  
Ratih Novita Praja ◽  
Wiwiek Tyasningsih ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Adult Female ◽  
Rose Bengal ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Descriptive Analysis ◽  
Fixation Test ◽  
Analysis Method ◽  
Rose Bengal Test

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.

scholarly journals Detection of Brucellosis in Dairy Cattle in Turen District Malang Regency using Rose Bengal Test (RBT) and Complement Fixation Test (CFT) Methods

2021 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Imbi Kusumastuti ◽  
Wiwiek Tyasningsih ◽  
Ratih Novita Praja ◽  
Suwarno Suwarno ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Positive Result ◽  
Rose Bengal ◽  
Complement Fixation Test ◽  
Total Population ◽  
Complement Fixation ◽  
Fixation Test ◽  
Rose Bengal Test ◽  
Confirmation Test ◽  
Positive Results

The purpose of this research was to detect Brucellosis in female dairy cattle in Turen District, Malang Regency. A total of 78 samples were used in this research taken from 351 total population of female dairy cattle that have been calculated based on the slovin formula. The methods used in this research were Rose Bengal Test (RBT) for the screening test and continued with Complement Fixation Test (CFT) for the confirmation test. The results RBT of this research clearly showed that positive results from 10 samples were confirmed as a final diagnostic test of Brucellosis using CFT. CFT showed a positive result from 9 samples and negative in 1 sample. The percentage of Brucellosis occurrence in the Turen District was recorded at 11.5%. Moreover, Turen District also became an area with a high Brucellosis occurrence (>2%).

scholarly journals Evaluation of Two Absorbed Enzyme-Linked Immunosorbent Assays and a Complement Fixation Test as Replacements for Fecal Culture in the Detection of Cows Shedding Mycobacterium Avium Subspecies Paratuberculosis

2002 ◽  
Vol 14 (3) ◽  
pp. 219-224 ◽  
Author(s):  
C. H. J. Kalis ◽  
H. W. Barkema ◽  
J. W. Hesselink ◽  
C. van Maanen ◽  
M. T. Collins
Keyword(s):  
Mycobacterium Avium ◽  
Detection Rate ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Fixation Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fecal Culture ◽  
Fecal Samples ◽  
Serologic Tests

Control of paratuberculosis in dairy herds is based on preventing the transmission of Mycobacterium avium subsp. paratuberculosis ( Mptb) from cows to calves by management measures, supported by removal of cows excreting these bacteria by the fecal route ( Mptb shedders). Fecal culture is the most accurate test for identifying Mptb shedders, but this technique is expensive and takes up to 16 weeks for results to be available. Serologic tests are inexpensive, rapid, and easy to perform. Of serologic tests, the complement fixation test (CFT) and absorbed enzyme-linked immunosorbent assay (ELISA) are the serologic tests used most frequently; the CFT is considered less accurate than the ELISA with respect to sensitivity and specificity. The commonly accepted absorbed ELISA is from the Australian Central Serum Laboratory. However, a European supplier has marketed a second ELISA that is supposed to be more sensitive in detecting Mptb shedders. These 2 absorbed ELISAs, designated ELISA-A and ELISA-B, and an in-house CFT were compared with data from 2 serum panels. The Mptb shedding panel consisted of sera from 198 culture-positive cows from 53 infected herds. The method used for culture of fecal samples was a modified Jørgensen method on individual samples. The Mptb shedder detection rate by the 3 serologic tests ranged from 29.8% to 39.4%. Detection rate for ELISA-A was lower than that for ELISA-B and CFT. For all 3 tests, detection rate was dependent on the level of Mptb shedding and the age of the animals. Detection rates increased as cattle age increased to 4 years. The specificity panel was initially composed of sera from 811 cows randomly selected from 41 herds without clinical paratuberculosis that were negative for Mptb based on whole-herd fecal culture. The modified Jørgensen method for culture was used on pooled fecal samples. Serologic test specificity ranged from 93.4% to 99.8%. The specificity of ELISA-A was higher than that of ELISA-B and CFT. Specificity of ELISA-B between herds was 75–100%. Specificity of CFT between herds was 62–100%. The low specificity of ELISA-B and CFT could not be explained by a higher sensitivity for Mptb-infected cows before onset of shedding, because in the 19 herds with 8 more subsequent negative whole-herd fecal cultures in the 4 years after sampling, specificity was not improved. The insufficient specificity of ELISA-B was not corrected sufficiently by heightening the cutoff value because Mptb shedder detection rate was lowered to 28.9%, equal to that of ELISA-A, and specificity only rose to 97%, much lower than that of ELISA-A. Taking into account the different test characteristics, serologic tests are a cost-effective alternative to fecal culture in high-prevalence herds. For certification programs, only ELISA-A is recommended because in a large number of nonsuspect herds specificity remained almost 100%.

scholarly journals Serological Detection of Q–Fever in the Sera of Humans and Ruminants in Selected Areas in the Philippines

2016 ◽  
Vol 18 (1) ◽  
Author(s):  
Fernando C. Cardona
Keyword(s):  
Coxiella Burnetii ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Q Fever ◽  
Zoonotic Disease ◽  
The Philippines ◽  
Fixation Test ◽  
Serological Detection ◽  
Sera Samples

Q fever is an emerging worldwide zoonotic disease caused by a bacterium Coxiella burnetii.  Detection of Q fever antibodies among humans and animals was conducted using sera from human donors and sera from three different species of animals obtained from Catarman, Northern Samar, Los Baños, Laguna, and General Santos City, South Cotabato, Philippines.  The Complement Fixation Test (CFT) was used to detect antibodies in sera samples.  The result of the study showed that Q fever antibodies in humans were found to be 37% (22/60) while the sampled animals have 43 % (77/180).  Among the animals, the highest occurrence of antibodies was observed in goats with 70% (42/60), followed by 40% among cattle (24/60) and the least 18% were from carabaos (11/60). According to sampling sites, 20% (12/60) of antibody-positive humans were from Los Baños, Laguna, some 17% (10/60) came from General Santos City and none from Catarman, Northern Samar.  Among the goats examined, a higher percentage of Q fever antibodies were obtained from those in Los Baños with 30% (18/60) occurrence, and from General Santos City with 25% (15/60) occurrence. Likewise, an equal occurrence rates of Q fever antibodies among cattle came from Los Baños, Laguna and General Santos City each with 20% (12/60) seropositivity, respectively. Among the carabaos, 18% (11/60) was in General Santos City and 8% (5/60) in Catarman. Based on these findings, it could be inferred that Q fever infection exists among humans, goats, cattle, and carabaos in the Philippines.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

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