scholarly journals Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test

2021 ◽  
Vol 4 (2) ◽  
pp. 199
Author(s):  
Agung Jati Kusuma ◽  
Erma Safitri ◽  
Ratih Novita Praja ◽  
Wiwiek Tyasningsih ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Adult Female ◽  
Rose Bengal ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Descriptive Analysis ◽  
Fixation Test ◽  
Analysis Method ◽  
Rose Bengal Test

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.

scholarly journals Detection of Brucellosis in Dairy Cattle in Turen District Malang Regency using Rose Bengal Test (RBT) and Complement Fixation Test (CFT) Methods

2021 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Imbi Kusumastuti ◽  
Wiwiek Tyasningsih ◽  
Ratih Novita Praja ◽  
Suwarno Suwarno ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Positive Result ◽  
Rose Bengal ◽  
Complement Fixation Test ◽  
Total Population ◽  
Complement Fixation ◽  
Fixation Test ◽  
Rose Bengal Test ◽  
Confirmation Test ◽  
Positive Results

The purpose of this research was to detect Brucellosis in female dairy cattle in Turen District, Malang Regency. A total of 78 samples were used in this research taken from 351 total population of female dairy cattle that have been calculated based on the slovin formula. The methods used in this research were Rose Bengal Test (RBT) for the screening test and continued with Complement Fixation Test (CFT) for the confirmation test. The results RBT of this research clearly showed that positive results from 10 samples were confirmed as a final diagnostic test of Brucellosis using CFT. CFT showed a positive result from 9 samples and negative in 1 sample. The percentage of Brucellosis occurrence in the Turen District was recorded at 11.5%. Moreover, Turen District also became an area with a high Brucellosis occurrence (>2%).

scholarly journals Seroprevalensi Brucellosis pada Sapi Potong di Papua Barat

2021 ◽  
Vol 2 (1) ◽  
pp. 128-133
Author(s):  
Sulaxono Hadi ◽  
Ratna Loventa Sulaxono
Keyword(s):  
Rose Bengal ◽  
Brucella Abortus ◽  
Screening Test ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Rose Bengal Test ◽  
Papua Barat

Brucellosis merupakan penyakit bakterial yang disebabkan oleh Brucella abortus, yang menga-kibatkan kerugian ekonomi akibat terjadinya keguguran pada sapi betina yang bunting pada triwulan ketiga kebuntingan. Surveilans brucellosis dalam mendukung pengembangan peternakan sapi di Propinsi Papua Barat telah dilakukan oleh Balai Besar Veteriner Maros. Tujuan pelaksanaan surveilans ini adalah untuk mengetahui seroprevalensi brucellosis pada sapi potong di Papua Barat. Surveilans dilakukan di 4 kabupaten/kota, 14 kecamatan dan 33 desa. Sebanyak 684 sampel serum sapi telah diambil guna pengujian terhadap brucellosis. Sampling dilakukan pada lokasi kabupaten, kecamatan dan desa terpilih yang padat ternak. Metode pengujian dilakukan secara seri, dengan melakukan screening test menggunakan Rose Bengal Test, yang bila hasilnya positif diuji lebih lanjut dengan Complement Fixation Test. Hasil pengujian menunjukkan adanya reaktor brucellosis pada 5 ekor dari 684 ekor sapi yang diambil dan diuji serumnya. Seroprevalensi brucellosis pada sapi sebesar 0,73% di desa Remu Utara, kecamatan Sorong, kota Sorong sebanyak 1 ekor serta di desa KaliMerah, kecamatan Masni, kabupaten Manokwari. Untuk mencegah penularan dan penyebaran brucellosis pada sapi maka sapi reaktor brucellosis segera dipotong dengan pengawasan dari petugas.

scholarly journals Deteksi Brucellosis pada Babi secara Serologis dan Molekuler di Rumah Potong Hewan Kapuk, Jakarta dan Ciroyom, Bandung

2018 ◽  
Vol 5 (2) ◽  
pp. 66-73
Author(s):  
Dina Kartini ◽  
Susan Maphilindawati Noor ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Polymerase Chain Reaction ◽  
Rose Bengal ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Chain Reaction ◽  
Rose Bengal Test ◽  
Brucella Suis ◽  
Polymerase Chain

Brucellosis pada babi merupakan penyakit zoonosis yang disebabkan oleh bakteri Brucella suis. Penyakit ini telah terdeteksi di Indonesia, namun belum banyak diteliti. Pada penelitian ini dilakukan deteksi brucellosis pada babi secara serologis menggunakan metode Rose Bengal Test (RBT) dan Complement Fixation Test (CFT) serta secara molekuler menggunakan teknik Polymerase Chain Reaction (PCR) AMOS untuk mengetahui spesies Brucella yang menginfeksi babi. Sampel penelitian berupa serum dan limfonodus dikoleksi dari 2 Rumah Potong Hewan Kapuk, Jakarta dan Ciroyom, Bandung. Hasil  pengujian secara serologis menunjukkan bahwa brucellosis tidak ditemukan pada sampel babi dari Rumah Potong Hewan Kapuk, Jakarta. Sementara hasil pengujian secara serologis dari Rumah Potong Hewan Ciroyom, Bandung menunjukkan 2,5% (1/40) positif dan 7,5%(3/40) positif PCR. Hasil PCR positif menunjukkan terdeteksi 2 spesies Brucella (B. abortus dan B. suis) pada 2 sampel organ limfonodus dan Brucella suis pada satu sampel limfonodus, sedangkan dari organ limpa terdektesi dua sampel positif Brucella suis. Hal ini menunjukkan bahwa selain Brucella suis, Brucella abortus juga menginfeksi babi pada penelitian ini.

scholarly journals Kondisi Brucellosis setelah Vaksinasi di Kecamatan Majauleng Kabupaten Wajo Sulawesi Selatan

2021 ◽  
Vol 2 (1) ◽  
pp. 114-119
Author(s):  
Sulaxono Hadi ◽  
Ratna Loventa Sulaxono ◽  
Siswani
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Rose Bengal Test

Vaksinasi brucellosis telah dilakukan secara massal di Kabupaten Majauleng, Kabupaten Wajo, Sulawesi Selatan tahun 2013 dan 2014 menggunakan vaksin brucellosis RB51. Vaksinasi dimaksudkan untuk mengendalikan dan menekan angka prevalensi yang tinggi di Kabupaten Wajo yang mencapai angka 30%. Surveilans telah dilakukan oleh Balai Besar Veteriner Maros untuk melihat dan mengevaluasi prevalensi brucellosis yang terjadi beberapa tahun kemudian untuk melihat penurunan prevalensi brucellosis pasca vaksinasi. Pada tahun 2016 telah dilakukan pengujian sampel serum yang berasal dari Kabupaten Wajo. Sebanyak 118 serum telah diuji dengan Rose Bengal Test (RBT) yang diteruskan dengan Complement Fixation Test (CFT). Hasilnya menunjukkan bahwa brucellosis masih ditemukan di Kabupaten Wajo dengan angka prevalensi sebesar 25,42%. Prevalensi terbesar ditemukan di Kecamatan Takalalla yang mencapai 73,68%, sedangkan di Kecamatan Majauleng sebesar 3.13%. Sampling lanjutan dilakukan pada tahun 2020 di kecamatan terpadat populasi sapinya yaitu Kecamatan Majauleng. Prevalensi brucellosis pada tahun 2020 di dua desa di Kecamatan Majauleng meningkat menjadi sebesar 6 %.

scholarly journals Serodeteksi Brucellosis dengan Metode Rose Bengal Test dan Complement Fixation Test pada Sapi Perah di Banyuwangi

2020 ◽  
Vol 3 (2) ◽  
pp. 188
Author(s):  
Estiary Wilujeng ◽  
Suwarno Suwarno ◽  
Ratih Novita Praja ◽  
Iwan Sahrial Hamid ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Rose Bengal Test

Tujuan penelitian adalah untuk mendeteksi adanya antibodi dari Brucella pada ternak sapi perah betina di Banyuwangi menggunakan uji Rose Bengal Test (RBT) untuk uji cepat dan dikonfirmasi dengan menggunakan Complement Fixation Test (CFT). Sebanyak 84 sampel dari 516 sapi perah betina di Banyuwangi, kemudian diambil secara acak. Sampel yang diambil berasal dari serum sapi perah betina. Hasil penelitian ditemukan dua sampel yang dinyatakan positif Brucellosis dengan uji RBT dan CFT. RBT merupakan uji screening yang dapat memberikan hasil positif palsu, dan kemudian dikonfirmasi dengan uji CFT untuk melihat titer hasil vaksin atau infeksi alam. Hasil positif pada uji RBT memiliki hubungan dengan hasil titer CFT karena titer antibodi pada RBT dapat diukur dari derajat aglutinasi yang terbentuk. Hasil seropositif penyakit Brucellosis di Banyuwangi sebesar 2%.

scholarly journals Identifikasi Reaktor Brusellosis Pada Populasi Sapi di Rumah Potong Hewan (RPH) Kota Ambon

2020 ◽  
Vol 3 (2) ◽  
pp. 39-44
Author(s):  
Astri Dwyanti Tagueha
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Program Monitoring ◽  
Rose Bengal Test

Brusellosis merupakan penyakit ekonomis pada hewan yang bersifat infeksius dan mudah menyebar. Evaluasi keberadaan reaktor di titik penyebaran seperti RPH penting untuk dilakukan. Penelitian ini bertujuan untuk mengetahui prevalensi reaktor brusellosis pada sapi di RPH Kota Ambon. Variabel yang diamati yaitu asal sapi, umur, jenis kelamin, dan status kebuntingan. Besaran sampel ditentukan berdasarkan rumus deteksi penyakit dengan tingkat konfidensi 95%, asumsi prevalensi 2%, dan error 0,05. Sebanyak 175 sampel darah diambil untuk pemeriksaan Rose Bengal Test (RBT) dan dikategorikan reaktor jika muncul reaksi aglutinasi pada saat pengujian. Hasil penelitian menunjukkan 10.29% sapi adalah reaktor. Diantara sejumlah sapi yang berstatus reaktor, 55.56% berasal dari Pulau Seram, 88.89% berumur > 1,5 tahun, 66.67% adalah betina, dan 75% positif bunting. Hasil ini perlu divalidasi dengan Complement Fixation Test (CFT) sehingga dapat dipakai sebagai bahan pertimbangan dalam merumuskan program monitoring dan survailans secara berkelanjutan

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

scholarly journals The study of epizootic sera obtained from ruminant animals in complement fixation test (CFT) with the use of Paratuberculous antigen

2019 ◽  
pp. 41-45
Author(s):  
A. I. Zavgorodniy ◽  
S. A. Pozmogova ◽  
N. V. Goncharova ◽  
M. V. Kalashnyk ◽  
V. V. Bilushko
Keyword(s):  
Dairy Cattle ◽  
Culture Filtrate ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Fixation Test ◽  
Laboratory Diagnostics ◽  
Serological Studies ◽  
Methodological Guidelines ◽  
Sera Samples

The paper presents results of the study of epizootic blood sera in the complement fixation test (CFT) with paratuberculous antigen. Blood sera were sampled from the cattle and goats. The antigen was produced from the culture filtrate of Mycobacterium avium subspecies paratuberculosis (MAP) in the laboratory for tuberculosis study. The aim of the present study was to clarify the epizootic situation concerning Johne’s disease among the dairy cattle in different regions of Ukraine. To achieve this aim the blood sera from cattle and goats were collected from farms in different regions of Ukraine. Those sera samples were studied in the complement fixation test with the use of paratuberculous antigen that was produced from the culture filtrate of MAP. The above mentioned blood sera were collected from the cattle that had positive allergic reactions on the use of tuberculin (PPD) for mammals. Those animals belonged to the free from tuberculosis and paratuberulosis milk farms. The study of obtained samples of blood sera was conducted in the accordance with the methodological guidelines “Laboratory diagnostics of paratuberculosis” (shutter. NMR FEFU pr. No. 1, dated December 19, 2014). There were studied 1098 blood sera samples from cattle. In addition to this, investigation was conducted on 24 samples of blood sera from goats. As the result of conducted study it was found that 17 samples of blood sera contained specific antibodies against MAP (serum solution 1:10). These blood sera collected from the cattle belonging to 4 farms in Poltava, Donetsk and Khmelnitsky regions. Along with this it was obtained 9 uncertain results in compliment fixation test that was conducted between paratuberculous antigen (ACF) and blood sera from those 4 farms. The results of monitoring studies indicate that M. avium subsp. paratuberculosis pathogen circulates in studied farms. This can lead to the complication of the epizootic situation regarding paratuberculosis and contribute to the spreading of this pathogen to other free from MAP infection farms. There are no anti-paratuberculosis antibodies in blood serum from goats. It is necessary to conduct annual monitoring serological studies of productive dairy cattle and imported animals in order to clarify and control epizootic situation concerning paratuberculosis on the territory of Ukraine

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