AN EPIDEMIOLOGY-CLINICAL CORRELATION OF VIRAL LOAD AS MEASURED BY CYCLE THRESHOLD VALUE BY RT-PCR IN COVID-1

2021 ◽  
pp. 1-2
Author(s):  
Atrikumar P. Patel ◽  
Palak Shah ◽  
Pavan Acharya ◽  
Monila N. Patel
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Load ◽  
Severe Acute Respiratory Syndrome ◽  
Threshold Value ◽  
Clinical Correlation ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Oropharyngeal Swab ◽  
Polymerase Chain ◽  
Novel Coronavirus

The 2019 novel coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] was rst documented in December 2019 in Wuhan, China, and spread across the globe resulting in [1]. signicant global morbidity and mortality Diagnosis of COVID-19 is mainly done by nasopharyngeal and oropharyngeal swab RT-PCR (Reverse transcriptase - polymerase chain reaction). Real time RT-PCR is of great interest today for detection of SARS- CoV-2 due to its benets as a specic assay.

scholarly journals Methods of Diagnosis a Preferential Advantage in COVID-19: A Mini Review

2021 ◽  
pp. 173-179
Author(s):  
Neha Saini ◽  
Prem Pandey ◽  
Mandar Shirolkar ◽  
Atul Kulkarni
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Review Article ◽  
Diagnostic Methods ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Health Crisis ◽  
The World ◽  
Polymerase Chain ◽  
Novel Coronavirus

Humanity is going through never seen before health crisis due to the outbreak of novel coronavirus or Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). There are 24.02 million cases and 0.82 million deaths worldwide as of 26th August 2020 due to deadly infection of COVID-19. The disease has been spreading exponentially (R-naught number: 3) and has challenged even the best healthcare infrastructure in the world. With the progression of the disease, the countries shifted the focus from cure to diagnosis and containment to flatten the curve. The review shows that the disease is spreading exponentially while the resources are still limited. We focus upon the probable vectors of the virus, different diagnostic methods with advantages & limitations, and the way forward. This review article covers the different diagnostic methods with more advantages, limitations, and the future sneak-peek into the forthcoming developments for the diagnostic processes such as RT-PCR (Reverse Transcription Polymerase chain reaction).

scholarly journals Performance of Oropharyngeal Swab Testing Compared With Nasopharyngeal Swab Testing for Diagnosis of Coronavirus Disease 2019—United States, January 2020–February 2020

2020 ◽  
Author(s):  
Monita R Patel ◽  
Darin Carroll ◽  
Emily Ussery ◽  
Hilary Whitham ◽  
Christopher A Elkins ◽  
...  
Keyword(s):  
United States ◽  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Nasopharyngeal Swab ◽  
Threshold Values ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Illness Onset ◽  
Oropharyngeal Swab ◽  
Polymerase Chain

Abstract Among 146 nasopharyngeal (NP) and oropharyngeal (OP) swab pairs collected ≤7 days after illness onset, Real-Time Reverse Transcriptase Polymerase Chain Reaction assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 RT-PCR) diagnostic results were 95.2% concordant. However, NP swab cycle threshold values were lower (indicating more virus) in 66.7% of concordant-positive pairs, suggesting NP swabs may more accurately detect the amount of SARS-CoV-2.

Multisystem Inflammatory Syndrome in Infant with Negative SARS-CoV-2 RT-PCR and Antibodies

2021 ◽  
pp. 35-39
Author(s):  
Hanna Sahhar ◽  
Karly Derwitz ◽  
Erica Rubin
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Early Diagnosis ◽  
World Health ◽  
Rt Pcr ◽  
Chain Reaction ◽  
The World ◽  
Polymerase Chain ◽  
Health Organization ◽  
Inflammatory Syndrome

Since the declaration of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in March 2020 by the World Health Organization (WHO), there has been an emergence of a new syndrome termed multisystem inflammatory syndrome in children (MIS-C) associated with COVID-19. MIS-C is defined by the presence of fever, systemic inflammation and multiorgan dysfunction in association with SARS-CoV-2 infection or COVID-19 exposure. Knowledge of this syndrome’s presentation and pathophysiology is constantly evolving as more cases are reported in the literature. This case identifies a 3-month-old patient who tested negative for SARS-CoV-2 antigen, reverse transcriptase polymerase chain reaction (RT-PCR) and antibodies but qualified for MIS-C diagnosis. To the best of our knowledge and through extensive research at the time of diagnosing and reporting this condition to the healthcare authorities, we report the youngest pediatric patient with MIS-C diagnosis. We document this case to contribute to further understanding the variable manifestations of MIS-C and the importance of early diagnosis and treatment with intravenous immunoglobulin (IVIG).

scholarly journals Covid-19 in outpatients—Is fever a useful indicator for SARS-CoV-2 infection?

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246312
Author(s):  
Anne Schneider ◽  
Holger Kirsten ◽  
Franziska Lordick ◽  
Florian Lordick ◽  
Christoph Lübbert ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Body Temperature ◽  
Final Analysis ◽  
University Hospital ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Course Of Illness ◽  
Oropharyngeal Swab ◽  
Polymerase Chain ◽  
Telephone Calls

Objective Understanding mild to moderate symptoms of coronavirus disease 2019 (Covid-19) is important in order to identify active cases early and thus counteract transmission. Methods In March 2020, Leipzig University Hospital established an outpatient clinic for patients potentially infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Confirmed cases with mild to moderate symptoms self-isolated at home and were followed-up by daily telephone calls for at least 14 days. Symptoms and course of illness of these patients are reported here. Results From March 20 to April 17, 2020, 1460 individuals were tested for SARS-CoV-2 by naso- or oropharyngeal swab for real-time polymerase chain reaction (RT-PCR). Covid-19 was confirmed in 91 (6.2%) patients, of which 87 were included in the final analysis. Patients presented for testing after a mean of 5.9 days (IQR = 2.0–8.5). The median age was 37.0 years (IQR = 28.5–53), and 48 (55.2%) were female. Five (5.7%) patients required hospital admission during the course of illness. Most frequently reported symptoms were fatigue (n = 64, 74%), cough (n = 58, 67%), and hyposmia/hypogeusia (n = 44, 51%). In contrast to previous reports, fever occurred in less than a third of patients (n = 25, 29%). By day 14, more than half of the patients had recovered completely (n = 37/70, 52.9%). Conclusions Fever seems to be less common in patients of relatively young age diagnosed with mild to moderate Covid-19. This suggests that body temperature alone may be an insufficient indicator of SARS-CoV-2 infection.

scholarly journals Multiple Negative of RT-PCR Testing of COVID-19 Pneumonia: A Case Report

2020 ◽  
Author(s):  
HuiLi ◽  
Junton Feng ◽  
Lamei Chen ◽  
Yinhao Wu ◽  
Yan Su ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Case Report ◽  
Reverse Transcriptase ◽  
Clinical Manifestation ◽  
Lymphocyte Count ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Case Presentation ◽  
Polymerase Chain ◽  
Novel Coronavirus

Abstract Background: Since the 2019 novel coronavirus outbreak in Wuhan, Hubei Province, China in 2019, there have been a few reports of multiple negative of RT-PCR tests in patients infected by 2019 novel coronavirus.Case presentation: The patient was a 64-year-old man with fever. His son returned from Hubei 17 days before the patient had fever. Ancillary examinations indicated a decreased lymphocyte count and ground-glass opacities in lung. However, the first five reverse transcriptase polymerase chain reaction tests of 2019 novel coronavirus were negative until the sixth turned to positive.Conclusion: when epidemiological history, clinical manifestation and imaging are highly suggestive of COVID-19 Pneumonia, we should repeat reverse transcriptase polymerase chain reaction tests and treat patients in isolation.

scholarly journals Laboratory Detection of Novel Corona Virus 2019 using Polymerase Chain Reaction

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Shafiq Ahmad Chudhary ◽  
Shahid Imtiaz ◽  
Nedal Iqbal
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Plasma Levels ◽  
Viral Infections ◽  
Rna Viruses ◽  
Chain Reaction ◽  
Chinese City ◽  
Laboratory Investigations ◽  
Polymerase Chain ◽  
Novel Coronavirus

Since mid-December 2019, several cases of a pneumonia like disease (with symptoms including fever, difficulty in breathing, cough and invasive lesions on both lungs) of unknown cause have emerged in the central Chinese city of Wuhan. Chinese authorities made a preliminary determination that the causative agent is a novel coronavirus(2019-nCoV). Coronaviruses are enveloped RNA viruses belonging to Coronaviridae family and the order Nidovirales. This subfamily consists of four genera alphacoronavirus, betacoronavirus, gammacoronavirus and deltacoronavirus on the basis of their phylogenetic relationships and genomic structures. These subfamilies are broadly distributed for causing infections in humans and other mammals. The alphacoronaviruses and betacoronaviruses infect only mammals. The gammacoronaviruses and deltacoronaviruses infect birds, but some of them can also infect mammals. The source of betacoronavirus 2019-nCoV is still unknown, although initial cases have been linked with south Huanan seafood market. Viral infections already known to produce similar symptoms are influenza, parainfluenza, Middle East respiratory syndrome (MERS-CoV) and severe acute respiratory syndrome (SARS-CoV). Laboratory investigations reported raised plasma levels of L2, IL7, IL10, GSCF, IP10, MCP1, MIP1A, and TNF? in patients.

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