scholarly journals MICROBIAL COMPOSITION OF VARIOUS SURFACES OF SKIN DURING DEVELOPMENT OF ATOPIC DERMATITIS BASED ON DATA FROM MALDI-TOF MASS-SPECTROMETRY IDENTIFICATION METHOD

Author(s):  
Yu. A. Tyurin ◽  
R. S. Fassakhov ◽  
T. V. Grigorieva ◽  
I. G. Mustafin

Aim. Study transformation of skin microflora during development of atopic dermatitis. Materials and methods. 45 patients with various forms of atopic dermatitis (AtD) were examined. Control group consisted of 26 healthy individuals. The strains were cultivated on elective nutrient media. Identification of the isolated strains was carried out by MALDI-TOF mass-spectrometry method. Results. A low frequency of occurrence of taxon Staphylococcus epidermidis on face skin and high frequency of occurrence of Staphylococcus aureus on upper and lower limb skin was established for AtD patients compared with healthy individuals. The frequency of occurrence of proteolytically active isolates of S. aureus in AtD patients was 3 times higher than in healthy carriers of this taxon. Taxons of microorganisms not inherent to healthy individuals such as Bacillus mycoides, Pseudomonas putida, Pseudomonas radiobacter were isolated on lower limb and neck skin of AtD patients. A high frequency of occurrence of Cryptococcus satoi, Candida albicans, Malassezia globosa fungi was noted. Conclusion. A decrease of barrier functions of skin during AtD facilitates contamination of patients’ skin with rare bacterial taxons and fungi. One of the possible mechanisms of suppression of immune competent cell functions could be proteolytic enzymes of S. aureus.

Mycoses ◽  
2013 ◽  
Vol 57 (3) ◽  
pp. 135-140 ◽  
Author(s):  
Stéphane Ranque ◽  
Anne-Cécile Normand ◽  
Carole Cassagne ◽  
Jean-Benjamin Murat ◽  
Nathalie Bourgeois ◽  
...  

2012 ◽  
Vol 159 (3-4) ◽  
pp. 544 ◽  
Author(s):  
Zoheira Djelouadji ◽  
Véronique Roux ◽  
Didier Raoult ◽  
Angeli Kodjo ◽  
Michel Drancourt

2016 ◽  
Vol 115 (11) ◽  
pp. 4199-4210 ◽  
Author(s):  
Bersissa Kumsa ◽  
Maureen Laroche ◽  
Lionel Almeras ◽  
Oleg Mediannikov ◽  
Didier Raoult ◽  
...  

Anaerobe ◽  
2012 ◽  
Vol 18 (3) ◽  
pp. 294-297 ◽  
Author(s):  
Rémi Fournier ◽  
Frédéric Wallet ◽  
Bruno Grandbastien ◽  
Luc Dubreuil ◽  
René Courcol ◽  
...  

2016 ◽  
Vol 26 (5) ◽  
pp. 69-73
Author(s):  
Lina Savickaitė ◽  
Jelena Kopeykinienė

Rapid identification of the infecting organism may aid in choosing appropriate antimicrobial therapy. We used MALDI-TOF mass spectrometry to identify bacteria directly from the positive blood culture samples (n=21). 85,71 percent of these results was identified using of MALDI-TOF mass spectrometry. Identification time of bacteria directly from the blood culture takes more than 1 hour for 27,8 percent results.


2019 ◽  
Vol 39 (2) ◽  
pp. 128-135
Author(s):  
Max Roberto Batista Araújo ◽  
Luisa Ferreira Seabra ◽  
Mireille Ângela Bernardes Sousa

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