scholarly journals In silico prediction of B- and T-cell epitopes in the CD2v protein of african swine fever virus (African swine fever virus, Asfivirus, Asfarviridae)

2020 ◽  
Vol 65 (2) ◽  
pp. 103-112 ◽  
Author(s):  
Ksenia A. Mima ◽  
Elena I. Katorkina ◽  
Sergey A. Katorkin ◽  
Sodnom Zh. Tsybanov ◽  
Aleksandr S. Malogolovkin
Keyword(s):  
T Cell ◽  
In Silico ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
T Cell Epitopes ◽  
In Silico Prediction ◽  
Cross Link ◽  
Mhc I ◽  
The Russian Federation

Introduction. African swine fever virus (ASF) is a large DNA virus that is the only member of the Asfarviridae family. The spread of the ASF virus in the territory of the Russian Federation, Eastern Europe and China indicates the ineffectiveness of existing methods of combating the disease and reinforces the urgent need to create effective vaccines. One of the most significant antigens required for the formation of immune protection against ASF is a serotype-specific CD2v protein.The purpose of the study. This study presents the results of immuno-informatics on the identification of B- and T-cell epitopes for the CD2v protein of the ASF virus using in silico prediction methods.Material and methods. The primary sequence of the CD2v protein of the ASFV virus strain Georgia 2007/1 (ID-FR682468) was analyzed in silico by programs BCPred, NetCTLpan, VaxiJen, PVS and Epitope Conservancy Analysis.Results. Using the BCPred and VaxiJen programs, 4 major B-cell immunogenic epitopes were identified. Analysis of the secretory region of ASF virus CD2v protein in NetCTLpan revealed 5 T-cell epitopes from the 32nd to the 197th position of amino acids that cross-link from the 1st to the 13th allele of the MHC-I of pigDiscussion. This study presents the results in silico prediction to identify B- and T-cell epitopes of ASF virus CD2v protein. The soluble region of the CD2v protein can be included in the recombinant polyepitope vaccine against African swine fever.Conclusion. B- and T-cell epitopes in the secretory region of the CD2v protein (from 17 to 204 aa) of ASF virus were identified by in silico prediction. An analysis of the conservatism of the identified B- and T-cell epitopes allowed us to develop a map of the distribution of immune epitopes in the CD2v protein sequence.

scholarly journals Identification of T-cell epitopes in African swine fever virus CD2v and C-type lectin proteins

2019 ◽  
Vol 100 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Galina Burmakina ◽  
Alexander Malogolovkin ◽  
Edan R. Tulman ◽  
Weidong Xu ◽  
Gustavo Delhon ◽  
...  
Keyword(s):  
T Cell ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
T Cell Epitopes

scholarly journals Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 29
Author(s):  
Laia Bosch-Camós ◽  
Elisabet López ◽  
María Jesús Navas ◽  
Sonia Pina-Pedrero ◽  
Francesc Accensi ◽  
...  
Keyword(s):  
T Cell ◽  
Mononuclear Cells ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Cd8 T Cell ◽  
Protective Role ◽  
Full Length ◽  
T Cell Epitopes ◽  
Peripheral Blood Mononuclear

The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.

scholarly journals Computational Analysis of African Swine Fever Virus Protein Space for the Design of an Epitope-Based Vaccine Ensemble

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1078 ◽  
Author(s):  
Albert Ros-Lucas ◽  
Florencia Correa-Fiz ◽  
Laia Bosch-Camós ◽  
Fernando Rodriguez ◽  
Julio Alonso-Padilla
Keyword(s):  
T Cell ◽  
Vaccine Development ◽  
De Novo ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Economic Losses ◽  
Virus Protein ◽  
Hemorrhagic Disease ◽  
Starting Point

African swine fever virus is the etiological agent of African swine fever, a transmissible severe hemorrhagic disease that affects pigs, causing massive economic losses. There is neither a treatment nor a vaccine available, and the only method to control its spread is by extensive culling of pigs. So far, classical vaccine development approaches have not yielded sufficiently good results in terms of concomitant safety and efficacy. Nowadays, thanks to advances in genomic and proteomic techniques, a reverse vaccinology strategy can be explored to design alternative vaccine formulations. In this study, ASFV protein sequences were analyzed using an in-house pipeline based on publicly available immunoinformatic tools to identify epitopes of interest for a prospective vaccine ensemble. These included experimentally validated sequences from the Immune Epitope Database, as well as de novo predicted sequences. Experimentally validated and predicted epitopes were prioritized following a series of criteria that included evolutionary conservation, presence in the virulent and currently circulating variant Georgia 2007/1, and lack of identity to either the pig proteome or putative proteins from pig gut microbiota. Following this strategy, 29 B-cell, 14 CD4+ T-cell and 6 CD8+ T-cell epitopes were selected, which represent a starting point to investigating the protective capacity of ASFV epitope-based vaccines.

scholarly journals Comparative in silico study of congocidine congeners as potential inhibitors of African swine fever virus

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221175
Author(s):  
Dickson Kinyanyi ◽  
Peris Amwayi ◽  
Mark Wamalwa ◽  
George Obiero
Keyword(s):  
In Silico ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
In Silico Study ◽  
Potential Inhibitors

scholarly journals Impaired T‐cell responses in domestic pigs and wild boar upon infection with a highly virulent African swine fever virus strain

2020 ◽  
Vol 67 (6) ◽  
pp. 3016-3032 ◽  
Author(s):  
Jane Hühr ◽  
Alexander Schäfer ◽  
Theresa Schwaiger ◽  
Laura Zani ◽  
Julia Sehl ◽  
...  
Keyword(s):  
T Cell ◽  
Wild Boar ◽  
Virus Strain ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
T Cell Responses ◽  
Fever Virus ◽  
Domestic Pigs ◽  
Cell Responses ◽  
Highly Virulent

scholarly journals In silico prediction and immunological validation of common HLA-DRB1-restricted T cell epitopes of Candida albicans secretory aspartyl proteinase 2

2008 ◽  
Vol 52 (4) ◽  
pp. 231-242 ◽  
Author(s):  
Songsak Tongchusak ◽  
Chanvit Leelayuwat ◽  
Vladimir Brusic ◽  
Sansanee C. Chaiyaroj
Keyword(s):  
Candida Albicans ◽  
T Cell ◽  
In Silico ◽  
T Cell Epitopes ◽  
In Silico Prediction
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