scholarly journals Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) - A Brief Review

2020 ◽  
Vol 05 (05) ◽  
pp. 230-232
Author(s):  
Dr. Pallavi BR
Keyword(s):  
Osmotic Fragility ◽  
Red Cell ◽  
Single Tube ◽  
Naked Eye

scholarly journals Role of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Detecting Beta-Thalassemia Trait

2018 ◽  
Vol 36 (4) ◽  
pp. 145-152 ◽  
Author(s):  
Asma Ferdousi ◽  
Mainuddin Ahmad ◽  
Jhulan Das Sharma ◽  
Rasheda Samad ◽  
AKM Zafar Ullah
Keyword(s):  
Predictive Value ◽  
Disease Incidence ◽  
Osmotic Fragility ◽  
Beta Thalassemia ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Naked Eye ◽  
Thalassemia Trait

Thalassemia is one of the commonest inherited diseases in Bangladesh. The birth of a thalassemic child places considerable strain, not only on the affected child and its family but also on the community and the nation at large. To reduce the burden of the society and to reduce the disease incidence by providing genetic counseling, detection of carrier is important. The present study evaluates the role of ‘Naked Eye Single Tube Red Cell Osmotic Fragility Test’ (NESTROFT) in detecting ß-thalassemia trait. The current study is a cross sectional study done during the period of September 2008 to August 2009. The study subjects were sibs, parents and relatives of thalassemia patients of age more than 1 year attending Pediatric department of Chittagong Medical College Hospital. Sample size was 50. Here subjects with lowered osmotic fragility test were detected and later on Hb- electrophoresis was done. All the data were recorded and analyzed by SPSS programme. The Sensitivity, Specificity and predictive value of positive and negative tests were computed and they were 92.6%, 80%, 92.6% and 80% respectively. False positive cases were found. The present study found NESTROFT to be both sensitive and reasonably specific and of high negative predictive value. However, multicenter study with large sample is needed to recommend NESTROFT as a single screening test for detection of ß-thalassemia trait.J Bangladesh Coll Phys Surg 2018; 36(4): 145-152

scholarly journals Evaluation of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Screening and Diagnosis of Beta Thalassaemia Trait in Pakistani Population

2021 ◽  
Keyword(s):  
Screening Test ◽  
Complete Blood Count ◽  
Osmotic Fragility ◽  
Rapid Screening ◽  
Red Cell ◽  
Pakistani Population ◽  
Single Tube ◽  
Beta Thalassaemia ◽  
Naked Eye ◽  
Thalassaemia Trait

Background: Beta thalassaemia is an inherited disease that is a cause of continuous burden on the affected families and the society. The objective of the study was to evaluate the efficacy of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) for the detection of beta thalassaemia trait when used for the screening in Pakistani population. Methods: All participants requesting a complete blood count were included in the study. Informed consent was obtained. Naked Eye Single Tube Red Cell Osmotic Fragility Test was performed with freshly prepared 0.36% buffered saline. Turbidity in the tubes was an indication for individuals to be positive for beta thalassaemia trait. Hemoglobin electrophoresis was performed on all positive cases to confirm the sensitivity and the reliability of the screening test. Chi-Square test was used to test for any significant correlation between the different hematological parameters and other variables. Results: A total of 812 patients were included in the study from January 2017 to June 2017. The mean age of participants was 35.5 + 13.6 years. There were 351 (43.2%) male and 461 (56.7%) female participants. A 100% Naked Eye Single Tube Red Cell Osmotic Fragility Test sensitivity was obtained with 65 (8%) positive cases having HbA2 levels of more than 3.2% indicating their true positivity whereas, all 747 (92%) negative cases having HbA2 levels of less than 3.2% indicating their true negativity. Conclusion: NESTROFT was found and a rapid screening test for the identification of carriers of beta thalassaemia trait in our population.

Validity of Naked Eye Single Tube Red Cell Osmotic Fragility Test for the Diagnosis of β-Thalassemia –A Case Control Study

2011 ◽  
Vol 24 (1) ◽  
pp. 1-5 ◽  
Author(s):  
MI Bari ◽  
LS Sharmin ◽  
PK Paul
Keyword(s):  
Negative Predictive Value ◽  
Predictive Value ◽  
Osmotic Fragility ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Medical College ◽  
Naked Eye ◽  
Sensitivity Specificity ◽  
Control Study

This study was conducted at the department of paediatrics, Rajshahi Medical College Hospital over a period of 2 years to see the sensitivity, specificity, positive predictive value and negative predictive value of Naked Eye Single Tube Red cell Osmotic Fragility (NESTROF) test for the diagnosis of β-thalassemia. Fifty eight children with β- thalassemia were taken as case and 58 children were taken as control. Among the control 29 children were normal (without anaemia) and 29 children had anaemia other than β-thalassemia. Hemoglobin electrophoresis was done to all children to enroll them as case or control. Then NESTROF test was performed in all cases and controls. The data was analyzed by SPSS V.12. This study showed that sensitivity, specificity, positive and negative predictive value of NESTROF test was 96.55%, 72.41%, 77.77% and 95.45% respectively. TAJ 2011; 24(1): 1-5

Hemoglobinopathy Screening by Osmotic Fragility Test Based on Flow Cytometer or Naked Eye

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Rinkle Mohapatra ◽  
Prashant Warang ◽  
Kanjaksha Ghosh ◽  
Roshan Colah
Keyword(s):  
Osmotic Fragility ◽  
Flow Cytometer ◽  
Naked Eye

scholarly journals Erythrocyte cellular and membrane deformability in hereditary spherocytosis

Blood ◽  
1979 ◽  
Vol 53 (3) ◽  
pp. 481-485 ◽  
Author(s):  
K Nakashima ◽  
E Beutler
Keyword(s):  
Cell Membrane ◽  
Hereditary Spherocytosis ◽  
Volume Ratio ◽  
Osmotic Fragility ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Relative Rigidity ◽  
Altered Surface ◽  
Red Cell Membranes ◽  
Expected Increase

In order to determine whether the relative rigidity of the hereditary spherocytosis (HS) red cell is due to membrane rididity or merely to an altered surface/volume ratio, we investigated the deformability of resealed red cell membranes from patients with HS. Whereas the osmotic fragility of intact red cells of HS patients showed the expected increase, the osmotic fragility of resealed HS membranes was normal, thus indicating that their surface/volume ratio was normal. Measurements with an ektacytometer showed that deformability of intact HS cells was markedly diminished, whereas deformability of resealed HS membranes was normal. These findings indicate that the HS red cell membrane is not intrinsically abnormally rigid, as has been suggested, but that the lack of deformability of the erythrocyte is primarily a function of the altered surface/volume ratio.

Hemoglobinopathy screening by osmotic fragility test based on flow cytometer or naked eye

2014 ◽  
Vol 90 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Rinkle Mohapatra ◽  
Prashant Warang ◽  
Kanjaksha Ghosh ◽  
Roshan Colah
Keyword(s):  
Osmotic Fragility ◽  
Flow Cytometer ◽  
Naked Eye

scholarly journals Anemia with Positive Direct Coombs' Test Induced by Trypan Blue

Blood ◽  
1961 ◽  
Vol 18 (5) ◽  
pp. 543-560 ◽  
Author(s):  
DAVID V. BROWN ◽  
EVA M. BOEHNI ◽  
LORNA M. NORLIND
Keyword(s):  
Lymph Nodes ◽  
Cell Survival ◽  
Trypan Blue ◽  
Osmotic Fragility ◽  
Red Cell ◽  
Coombs Test ◽  
Binding Properties ◽  
Wistar Strain ◽  
Red Cell Survival ◽  
Positive Direct

Abstract An anemia with a positive direct and indirect Coombs' test was induced in Wistar strain rats by injection of the dye trypan blue. The anemia was characterized by a shortened red cell survival time, increased osmotic fragility and reticulocytosis. There was an associated leukopenia and thrombocytopenia. The positive Coombs' tests were regarded as a nonimmunologic phenomena and attributed to the protein binding properties of the dye. Splenomegaly and lymphadenopathy were constant findings in the anemic animals. Splenectomy did not alter the course of the anemia. The pathway of erythrocyte destruction was thought to involve the sequestration and destruction of protein coated cells in the spleen, lymph nodes and liver.

scholarly journals Genetic differences in red cell osmotic fragility: analysis in allophenic mice

Blood ◽  
1982 ◽  
Vol 59 (5) ◽  
pp. 986-989 ◽  
Author(s):  
MJ Dewey ◽  
JL Brown ◽  
FS Nallaseth
Keyword(s):  
Phenotypic Variation ◽  
Blood Cells ◽  
Osmotic Fragility ◽  
Red Cells ◽  
Fragility Analysis ◽  
Red Cell ◽  
F1 Hybrid ◽  
Osmotic Lysis ◽  
Per Se ◽  
Genetically Determined

Abstract Mice of strain DBA/2J were found to produce red cells considerably more resistant to osmotic lysis than cells from C57BL/6J or the F1 hybrid between the two strains. Such strain-specific differences in osmotic fragility could be the result of genetically determined humoral or other systemic differences that indirectly influence red cell properties. Alternatively, this phenotypic variation might be an inherent property of the erythrocyte themselves and be directly controlled by their genotype. Analysis of red cells from allophenic (mosaic) mice of the strain composition C57BL/6J in equilibrium DBA/2J demonstrated that the latter possibility is the case. In such mice, erythrocytes of the DBA/2J genotype are relatively more resistant to osmotic lysis than are those of the C57BL/6J genotype; partial lysis of allophenic blood at intermediate salt concentrations results in marked enrichment for DBA/2J cells among the survivors. Future experiments designed to determine the mechanism underlying this difference can now focus on the properties of the red blood cells per se with the certainty that this property is inherent to the genotype of each cell.

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