scholarly journals Role of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Detecting Beta-Thalassemia Trait

2018 ◽  
Vol 36 (4) ◽  
pp. 145-152 ◽  
Author(s):  
Asma Ferdousi ◽  
Mainuddin Ahmad ◽  
Jhulan Das Sharma ◽  
Rasheda Samad ◽  
AKM Zafar Ullah
Keyword(s):  
Predictive Value ◽  
Disease Incidence ◽  
Osmotic Fragility ◽  
Beta Thalassemia ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Naked Eye ◽  
Thalassemia Trait

Thalassemia is one of the commonest inherited diseases in Bangladesh. The birth of a thalassemic child places considerable strain, not only on the affected child and its family but also on the community and the nation at large. To reduce the burden of the society and to reduce the disease incidence by providing genetic counseling, detection of carrier is important. The present study evaluates the role of ‘Naked Eye Single Tube Red Cell Osmotic Fragility Test’ (NESTROFT) in detecting ß-thalassemia trait. The current study is a cross sectional study done during the period of September 2008 to August 2009. The study subjects were sibs, parents and relatives of thalassemia patients of age more than 1 year attending Pediatric department of Chittagong Medical College Hospital. Sample size was 50. Here subjects with lowered osmotic fragility test were detected and later on Hb- electrophoresis was done. All the data were recorded and analyzed by SPSS programme. The Sensitivity, Specificity and predictive value of positive and negative tests were computed and they were 92.6%, 80%, 92.6% and 80% respectively. False positive cases were found. The present study found NESTROFT to be both sensitive and reasonably specific and of high negative predictive value. However, multicenter study with large sample is needed to recommend NESTROFT as a single screening test for detection of ß-thalassemia trait.J Bangladesh Coll Phys Surg 2018; 36(4): 145-152

Validity of Naked Eye Single Tube Red Cell Osmotic Fragility Test for the Diagnosis of β-Thalassemia –A Case Control Study

2011 ◽  
Vol 24 (1) ◽  
pp. 1-5 ◽  
Author(s):  
MI Bari ◽  
LS Sharmin ◽  
PK Paul
Keyword(s):  
Negative Predictive Value ◽  
Predictive Value ◽  
Osmotic Fragility ◽  
Red Cell ◽  
College Hospital ◽  
Single Tube ◽  
Medical College ◽  
Naked Eye ◽  
Sensitivity Specificity ◽  
Control Study

This study was conducted at the department of paediatrics, Rajshahi Medical College Hospital over a period of 2 years to see the sensitivity, specificity, positive predictive value and negative predictive value of Naked Eye Single Tube Red cell Osmotic Fragility (NESTROF) test for the diagnosis of β-thalassemia. Fifty eight children with β- thalassemia were taken as case and 58 children were taken as control. Among the control 29 children were normal (without anaemia) and 29 children had anaemia other than β-thalassemia. Hemoglobin electrophoresis was done to all children to enroll them as case or control. Then NESTROF test was performed in all cases and controls. The data was analyzed by SPSS V.12. This study showed that sensitivity, specificity, positive and negative predictive value of NESTROF test was 96.55%, 72.41%, 77.77% and 95.45% respectively. TAJ 2011; 24(1): 1-5

scholarly journals A Screening Test for Iron Deficiency Anaemia and Thalassaemia Traits

2015 ◽  
Vol 32 (4) ◽  
pp. 190-193
Author(s):  
Amin Lutful Kabir ◽  
Tashmim Farhana Dipta ◽  
Hajera Khatun ◽  
Mohammad Hafizur Rahman ◽  
Mahfuz Haq ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Predictive Value ◽  
Iron Deficiency Anaemia ◽  
Osmotic Fragility ◽  
Red Cell ◽  
Deficiency Anaemia ◽  
Final Study ◽  
Thalassaemia Trait ◽  
Study Population

Both iron deficiency and thalassaemia trait can present with hypochromic microcytic morphology. Naked eye single tube red cell osmotic fragility (NESTROF) is an inexpensive and simple test which can effectively differentiate iron deficiency anaemia from thalassaemia trait. Our study was an opportunity to observe the role of NESTROF in screening of thalassaemia trait among our study population. Initially NESTROF was carried out in 677 patients (N) population of four groups having both positive and negative NESTORF; for economical issue, auto-analyzer study and haemoglobin electrophoresis were done among only 100 randomly selected subjects, who were finally included in our study as final study population (n). In our study, NESTROF was 94.23% sensitive and its specificity was 92.08%. The predictive value of a positive test was 88.13% and predictive value of a negative test was 96.24%. Our study showed, NESTROF can effectively differentiate iron deficiency anaemia (IDA) from thalassaemia trait but, is not effective in co-existent IDA and thalassaemia.J Bangladesh Coll Phys Surg 2014; 32: 190-193

scholarly journals Evaluation of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) in Screening and Diagnosis of Beta Thalassaemia Trait in Pakistani Population

2021 ◽  
Keyword(s):  
Screening Test ◽  
Complete Blood Count ◽  
Osmotic Fragility ◽  
Rapid Screening ◽  
Red Cell ◽  
Pakistani Population ◽  
Single Tube ◽  
Beta Thalassaemia ◽  
Naked Eye ◽  
Thalassaemia Trait

Background: Beta thalassaemia is an inherited disease that is a cause of continuous burden on the affected families and the society. The objective of the study was to evaluate the efficacy of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) for the detection of beta thalassaemia trait when used for the screening in Pakistani population. Methods: All participants requesting a complete blood count were included in the study. Informed consent was obtained. Naked Eye Single Tube Red Cell Osmotic Fragility Test was performed with freshly prepared 0.36% buffered saline. Turbidity in the tubes was an indication for individuals to be positive for beta thalassaemia trait. Hemoglobin electrophoresis was performed on all positive cases to confirm the sensitivity and the reliability of the screening test. Chi-Square test was used to test for any significant correlation between the different hematological parameters and other variables. Results: A total of 812 patients were included in the study from January 2017 to June 2017. The mean age of participants was 35.5 + 13.6 years. There were 351 (43.2%) male and 461 (56.7%) female participants. A 100% Naked Eye Single Tube Red Cell Osmotic Fragility Test sensitivity was obtained with 65 (8%) positive cases having HbA2 levels of more than 3.2% indicating their true positivity whereas, all 747 (92%) negative cases having HbA2 levels of less than 3.2% indicating their true negativity. Conclusion: NESTROFT was found and a rapid screening test for the identification of carriers of beta thalassaemia trait in our population.

EVALUATION OF DIAGNOSTIC ACCURACY OF HEMATOLOGICAL INDICES IN THE DIFFERENTIATION OF BETA THALASSEMIA TRAIT FROM IRON DEFICIENCY ANEMIA

2021 ◽  
pp. 24-26
Author(s):  
Kavita Choudhary ◽  
M. L Gupta ◽  
Monika Hemin Shroff ◽  
Abhishek Rajawat
Keyword(s):  
Predictive Value ◽  
Hypochromic Anemia ◽  
Deficiency Anemia ◽  
Beta Thalassemia ◽  
Red Cell ◽  
Hematological Indices ◽  
Medical College ◽  
Red Cell Indices ◽  
Thalassemia Trait ◽  
Youden’S Index

Background: 2 most frequent type of microcytic hypochromic anemias are Iron deciency anemia (IDA) and beta thalassemia trait (β-TT) continue to be a cause of signicant burden to the society, particularly in developing countries like INDIA, where there is limitation of resources. The objective of the present study was to evaluate the validity of different discrimination indices to differentiate β-TTfromIDA. Methods:All diagnosed patients of microcytic hypochromic anemia admitted in Geetanjali Medical College and Hospital, Udaipur (Raj) between Jan 2018 to June 2020, were included in this study. Sensitivity, specicity, positive predictive value (PPV), negative predictive value (NPV) and Youden's index (YI) were calculated. Result: According to our study following rankings of various discrimination indices were obtained for BTT patients, RDW Index > Mentzer's Index = Ehsani Index > Sirdah Index > MDHLIndex > MCHD Index. Conclusion: According to our results and observations, the percentage of correctly diagnosed patients are the highest with RDW Index and Mentzer's Index. It should be kept in mind that several studies have shown that none of the red cell indices carry 100% sensitivity and specicity in diagnosing BTT.

scholarly journals An analysis of NESTROFT and Red cell indices in evaluating antenatal mothers for Beta Thalassemia trait

2018 ◽  
Vol 17 (3) ◽  
pp. 411-416
Author(s):  
Rana Safia ◽  
Zeeba S Jairajpuri ◽  
Shaan Khetrapal ◽  
MJ Hassan ◽  
Monika Gupta ◽  
...  
Keyword(s):  
Health Care ◽  
Low Cost ◽  
Care Center ◽  
Medical Science ◽  
Osmotic Fragility ◽  
Beta Thalassemia ◽  
Red Cell ◽  
Health Care Center ◽  
Red Cell Indices ◽  
Thalassemia Trait

Background: The frequency of thalassemia trait is about 3% worldwide, while in developing countries like India, it is a major cause of burden on the health care system. Naked eye single tube red cell osmotic fragility test (NESTROFT) used in population screening for beta thalassemia trait. It is a simple, low cost, reliable and most suitable screening test for β BTT, with a sensitivity of 99.8% in regions with high prevalence rates.Material &Methods: The present study was conducted in the Department of Pathology and included a total of 174 antenatal cases, attending the Out Patient Department of Obstetrics and Gynecology of a tertiary health care center in New Delhi between June 2011 to January 2012. The aim of the study was to screen mothers antenatally for early detection of BTT and to test the validity of NESTROFT in detection of thalassemic carriers of this area.Results: 174 pregnant women attending antenatal clinics were screened for detecting hemoglobinopathy with the help of NESTROFT, red cell indices, haemoglobin electrophoresis and HPLC It was seen that only four cases were BTT while ten were of microcytic anaemia and two had normal red cell indices.Conclusion: antenatal diagnosis of BTT is an important screening programme to reduce the burden of birth of children suffering from thalassemia and other hemoglobinopathies.Bangladesh Journal of Medical Science Vol.17(3) 2018 p.411-416

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