Effects of Egg Coverages on Storage and Pre-incubation Periods in Broiler Breeder Eggs

SummaryPrevious work from this department, concerned with testing the potential thrombogenicity of therapeutic factor IX concentrates, demonstrated that following recalcification of factor IX concentrates thrombin was generated within 3-30 minutes of incubation (Sas et al. 1975). The test developed (known as the TGt 50 test) is a two-stage assay and was thus found to be time consuming, tedious and tended to become inaccurate with long incubation periods and a large number of samples. A semiautomatic version of the test is reported in which the synthetic peptide Bz-ILE-GLU-GLY-ARG-pNA (S-2222) is added to recalcified, diluted factor IX concentrate in the micro-cuvette of a multiple sample recording spectrophotometer. Information can be obtained on (a) the amount of Xa (if any) present prior to recalcification (b) the initial amount of Xa formed and (c) the time taken to activate all factor X to Xa. Direct graphical interpretation shows a number of qualitative differences between commercial preparations, but by either of the criteria (b) or (c) above, it is possible to place the different products into “activated” and “non activated” groups such that both the Xa generation times and TGt 50 tests identify the same two groups of products. This agreement also indicates that the TGt 50 test is independent of the intrinsic factor V levels in the various concentrates.

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A Comparison of Incubation Periods for Bioburden Isolates

Biomedical Instrumentation & Technology ◽

10.2345/0899-8205(2006)40[67:acoipf]2.0.co;2 ◽

2006 ◽

Vol 40 (1) ◽

pp. 67-71 ◽

Cited By ~ 1

Author(s):

Trabue Bryans ◽

Karen Alexander

Keyword(s):

Incubation Periods

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Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology ◽

10.21608/eajbsc.2019.70775 ◽

2019 ◽

Vol 11 (3) ◽

pp. 139-147

Author(s):

Mona Azab ◽

Mohamed Zowail ◽

Nassif A. ◽

Ghada Elsadek ◽

Safwa Mohamed

Keyword(s):

Phylogenetic Analysis ◽

Salmonella Spp ◽

Broiler Breeder

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EFFECT OF ADDED ROCK PHOSPHATE AND COMPOST ON SOIL PHOSPHORUS FRACTIONS AFTER DIFFERENT INCUBATION PERIODS

Menoufia Journal of Soil Science ◽

10.21608/mjss.2020.117577 ◽

2020 ◽

Vol 5 (9) ◽

pp. 149-157

Author(s):

S. A. Radwan ◽

M. M. H. Shalaby ◽

W. A. Nada ◽

Soad M. EL-Ashry ◽

M. A. Abo Seeda ◽

...

Keyword(s):

Rock Phosphate ◽

Soil Phosphorus ◽

Phosphorus Fractions ◽

Soil Phosphorus Fractions ◽

Incubation Periods

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10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0042 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Kiptiyah Kiptiyah ◽

Widodo Widodo ◽

Gatot Ciptadi ◽

Aulanni’am Aulanni’Am ◽

Mohammad A. Widodo ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Culture ◽

Superoxide Dismutase ◽

In Silico ◽

Cumulus Cell ◽

Cumulus Cells ◽

Sod Activity ◽

In Silico Studies ◽

Incubation Periods

AbstractBackgroundWe investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells.MethodsFor the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.ResultsThe in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10˗gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.Conclusions10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.

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Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Animals ◽

10.3390/ani11040944 ◽

2021 ◽

Vol 11 (4) ◽

pp. 944

Author(s):

Giulia Quaglia ◽

Giulia Mescolini ◽

Elena Catelli ◽

Giacomo Berto ◽

Filippo Muccioli ◽

...

Keyword(s):

Dna Amplification ◽

Molecular Characteristics ◽

Distribution Map ◽

Present Survey ◽

Broiler Breeder ◽

Non Invasive ◽

Chicken Infectious Anemia Virus ◽

Chicken Infectious Anemia ◽

Anemia Virus ◽

Backyard Chicken

Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

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Interventions in mating strategies of broiler breeder during peak and post peak phase influence hatching traits

Poultry Science ◽

10.1016/j.psj.2021.101095 ◽

2021 ◽

pp. 101095

Author(s):

Muhammad Shabir Shaheen ◽

Shahid Mehmood ◽

Athar Mahmud ◽

Amjad Riaz ◽

Sohail Ahmad

Keyword(s):

Mating Strategies ◽

Broiler Breeder ◽

Peak Phase

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Data mining of coronavirus: SARS-CoV-2, SARS-CoV and MERS-CoV

BMC Research Notes ◽

10.1186/s13104-021-05561-4 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Jung Eun Huh ◽

Seunghee Han ◽

Taeseon Yoon

Keyword(s):

Machine Learning ◽

Amino Acid ◽

Amino Acid Sequence ◽

Decision Tree ◽

Machine Learning Algorithms ◽

High Similarity ◽

Incubation Periods ◽

Initial Question ◽

The Difference ◽

Blast Program

Abstract Objective In this study we compare the amino acid and codon sequence of SARS-CoV-2, SARS-CoV and MERS-CoV using different statistics programs to understand their characteristics. Specifically, we are interested in how differences in the amino acid and codon sequence can lead to different incubation periods and outbreak periods. Our initial question was to compare SARS-CoV-2 to different viruses in the coronavirus family using BLAST program of NCBI and machine learning algorithms. Results The result of experiments using BLAST, Apriori and Decision Tree has shown that SARS-CoV-2 had high similarity with SARS-CoV while having comparably low similarity with MERS-CoV. We decided to compare the codons of SARS-CoV-2 and MERS-CoV to see the difference. Though the viruses are very alike according to BLAST and Apriori experiments, SVM proved that they can be effectively classified using non-linear kernels. Decision Tree experiment proved several remarkable properties of SARS-CoV-2 amino acid sequence that cannot be found in MERS-CoV amino acid sequence. The consequential purpose of this paper is to minimize the damage on humanity from SARS-CoV-2. Hence, further studies can be focused on the comparison of SARS-CoV-2 virus with other viruses that also can be transmitted during latent periods.

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