scholarly journals Development of common wheat lines with the recombinant arm 1RS as a source of new combinations of disease and pest resistance genes

2016 ◽  
pp. 143-150
Author(s):  
N.A. Kozub ◽  
I.A. Sozinov ◽  
A.Ya. Bidnyk ◽  
N.A. Demianova ◽  
Ya.B. Blume ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Resistance Genes ◽  
Dna Markers ◽  
Recombinant Inbred Lines ◽  
Pest Resistance ◽  
Inbred Lines ◽  
New Combination ◽  
New Combinations ◽  
Rye Translocations ◽  
Wheat Lines

A combination of recombinant-inbred lines of the F6 generation from the cross B-16 ќ AR 7086 between lines with two wheat-rye translocations, 1BL/1RS from the Petkus and 1AL/1RS from the rye Insave, was developed. Using gliadin and secalin loci as genetic markers we identified recombinant arm 1RS in positions 1A and 1B in about 10% of lines. The rest of lines with the rye material may also carry recombinant 1RS, which can be identified with DNA markers. Lines with recombinant arm 1RS may serve as a source of new combination of rye genes for disease and pest resistance.

Determination of the composition and the allelic state of disease and pest resistance genes in potato parental lines using DNA markers

2016 ◽  
Vol 52 (5) ◽  
pp. 498-506 ◽  
Author(s):  
A. P. Yermishin ◽  
O. V. Svitoch ◽  
E. V. Voronkova ◽  
O. N. Gukasian ◽  
V. I. Luksha
Keyword(s):  
Resistance Genes ◽  
Dna Markers ◽  
Pest Resistance ◽  
Allelic State ◽  
Parental Lines

scholarly journals Development of a simultaneous detection method of DNA markers linked to four disease and pest resistance genes in potato using multiplex PCR

2010 ◽  
Vol 12 (1) ◽  
pp. 22-25
Author(s):  
Kazuyuki Mori ◽  
Kengo Ohbayashi ◽  
Seiji Tamiya ◽  
Yu Sakamoto ◽  
Nobuhiro Mukojima ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Resistance Genes ◽  
Dna Markers ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Pest Resistance

scholarly journals GRAS-Di system facilitates high-density genetic map construction and QTL identification in recombinant inbred lines of the wheat progenitor Aegilops tauschii

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuka Miki ◽  
Kentaro Yoshida ◽  
Hiroyuki Enoki ◽  
Shoya Komura ◽  
Kazuyo Suzuki ◽  
...  
Keyword(s):  
Flowering Time ◽  
Genetic Markers ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Aegilops Tauschii ◽  
Amplicon Sequencing ◽  
Inbred Lines ◽  
High Density ◽  
D Genome ◽  
Triticeae Species

AbstractDue to large and complex genomes of Triticeae species, skim sequencing approaches have cost and analytical advantages for detecting genetic markers and building linkage maps. Here, we develop a high-density linkage map and identify quantitative trait loci (QTLs) for recombinant inbred lines of Aegilops tauschii, a D-genome donor of bread wheat, using the recently developed genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) system, which facilitates skimming of the large and complicated genome and generates a large number of genetic markers. The deduced linkage groups based on the GRAS-Di genetic markers corresponded to the chromosome number of Ae. tauschii. We successfully identified stable QTLs for flowering time and spikelet shape-related traits. Genotype differences of RILs at the QTL-linked markers were significantly associated with the trait variations. In particular, one of the QTL-linked markers for flowering time was mapped close to VRN3 (also known as FLOWERING LOCUS T), which controls flowering. The GRAS-Di system is, therefore, an efficient and useful application for genotyping and linkage mapping in species with large and complex genomes, such as Triticeae species.

Molecular mapping of four blast resistance genes using recombinant inbred lines of 93-11 and nipponbare

2013 ◽  
Vol 56 (2) ◽  
pp. 91-97 ◽  
Author(s):  
Hongmei Yang ◽  
Melissa H. Jia ◽  
Yulin Jia ◽  
Junjie Xing ◽  
R. -C. Venu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Recombinant Inbred ◽  
Molecular Mapping ◽  
Blast Resistance ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Blast Resistance Genes

Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato

Euphytica ◽  
2011 ◽  
Vol 180 (3) ◽  
pp. 347-355 ◽  
Author(s):  
Kazuyuki Mori ◽  
Yu Sakamoto ◽  
Nobuhiro Mukojima ◽  
Seiji Tamiya ◽  
Takashi Nakao ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Resistance Genes ◽  
Dna Markers ◽  
Simultaneous Detection ◽  
Pest Resistance ◽  
Pcr Method

Diverse sources of resistance to Spodoptera litura (F.) in groundnut

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
M. A. Saleem ◽  
G. K. Naidu ◽  
H. L. Nadaf ◽  
P. S. Tippannavar
Keyword(s):  
Recombinant Inbred ◽  
Spodoptera Litura ◽  
Hot Spot ◽  
Recombinant Inbred Lines ◽  
Insect Pest ◽  
Inbred Lines ◽  
Leaf Damage ◽  
Botanical Variety ◽  
Resistant Genotypes ◽  
Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

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