scholarly journals ACTIVITY ASSAY OF SOME PROBIOTICS EXTRACT AS ANTI-Pseudomonas aeruginosa THAT ISOLATED FROM POSTOPERATIVE WOUND INFECTION IN ALNAJAF GOVRNORATE -IRAQ

2021 ◽  
Vol 4 (1) ◽  
pp. 16-19
Author(s):  
Suhad Mohammed ◽  
SH Ruaa ◽  
Basil Atheer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acetic Acid ◽  
Citric Acid ◽  
Wound Infection ◽  
Saccharomyces Boulardii ◽  
Diffusion Method ◽  
Postoperative Wound Infection ◽  
Birth Process ◽  
Disk Diffusion Method ◽  
Postoperative Wound

Background and objectives: Probiotics was redefined as “live microorganisms administered in inadequate amount that confer a health profit on the human for that the aim of this study were possibility of using probiotics extract with some supporting substances in antibacterial that resistant antibiotics. Materials and methods:The methods of this study included isolated and identification of bacteria from postoperative wound infection of 50 women subjected to surgical birth process then selected the P.aeruginosa isolates and scanning the sensitivity test of some cephalosporin type of antibiotics and determine the presence of blaOXA-1 gene, also the study had tested activity of two types of probiotics (Bacillus clausii and Saccharomyces boulardii )extract with some supporting substances (acetic acid and citric acid) as antibacterial. Results: The results of identification of bacterial isolates showed that 22(44%) isolates that related to Pseudomonas aeruginosa, 5(10%) isolates related to E.coli, 10(20%) isolates related to Klibsella spp., 10(20%) isolates related to Staphylococcus aureus and 3(6%) isolated related to Protus mirabils, and Overall, 22 isolates of P.aruginosa were analyzed by Kirby Bauer disk diffusion method and notice the resistance or sensitive to cephalexin, cefotriaxon and cefpime where the results confirmed 14 cephalosporin resistant isolates of Pseudomonas aeruginosa. Also detect blaOXA-1 gene by genotypic test. Amplification of b-lactamase genes shows the presence of blaOXA-1 (at 427 bp line) on among (10/22) study isolates. The results of antibacterial activity showed that inhibition zone of  probiotics extract only (14and 16)mm to S.bouldarii and B.clausii respectively but it showed synergism effect when supporting the S.bouldarii and B.clausii  extract with acetic acid where (18 and 20.3)mm respectively while (19 and 23.5)mm with citric acid respectively with significantly increasing differences when subjecting the bacteria to cefime ,ceftriaxone and cephlaxin antibiotics so,the inhibition zones were(1.3,10.3 and 0)mm respectively. Conclusion :In light of the foregoing, we concluded the possibility of using probiotic extract with some supported substances as an anti-bacterial Pseudomonas aeruginosa isolated from surgical wound infection.

Acetic acid treatment of pseudomonal postoperative wound infection

1997 ◽  
Vol 36 (3) ◽  
pp. 243-244 ◽  
Author(s):  
B.S. Nagoba ◽  
S.R. Deshmukh ◽  
B.J. Wadher ◽  
S.B. Patil
Keyword(s):  
Acetic Acid ◽  
Wound Infection ◽  
Acid Treatment ◽  
Postoperative Wound Infection ◽  
Postoperative Wound ◽  
Acetic Acid Treatment

scholarly journals Antibacterial Activity of Aloe vera Gel against Multidrug Resistant Staphylococcus aureus and Pseudomonas aeruginosa

2020 ◽  
pp. 74-80
Author(s):  
Usman A. ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Wound Infection ◽  
Aloe Vera ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Diffusion Technique ◽  
Aloe Vera Gel

Staphylococcus aureus and Pseudomonas aeruginosa have been implicated as important nosocomial pathogens causing severe infections especially in hospitalized patients. The aim of the study was to assess the antibacterial activity of Aloe vera gel against multidrug resistant S. aureus and P. aeruginosa isolated from wound. Clinical isolates of S. aureus and P. aeruginosa from wound infection were collected from Microbiology laboratory of Barau Dikko Teaching Hospital (BDTH), Kaduna and re-confirmed using standard microbiological procedure. Antibiotic susceptibility pattern of the isolates was determined using Kirby Bauer disk diffusion method. Aloe vera gel was obtained fresh matured leaves of Aloe vera plant and was screened for the presence of phytochemical constituents. Antibacterial activity of the Aloe vera gel against Multidrug Resistant (MDR) Staphylococcus aureus and Pseudomonas aeruginosa isolates was determined by agar well diffusion technique. The result revealed that all the two isolates were resistant to more than three classes of antibiotics. The Staphylococcus aureus isolate was resistant to fluoroquinolone (ciprofloxacin), aminoglycoside (gentamicin), cephalosporin (cefaroline), folate pathway antagonist (trimethoprim-sulfamethoxazole), penicillin(cefoxitin) and macrolide (erythromycin) while the Pseudomonas aeruginosa isolate was resistant to βlactam (ticarcillin-clavulanate), fluoroquinolone (ciprofloxacin and norfloxacin), aminoglycoside (gentamicin) and cephalosporin (ceftazidime)hence regarded as MDR isolates. Phytochemical screening of the gel revealed the presence of saponins, flavonoids, terpenoids and alkaloids. The Aloe vera gel was found to have antibacterial activity against the test isolates with MIC and MBC values of 25µg/mL and 50µg/mL against MDR S. aureus 50µg/mL and 100µg/mL against MDR P. aeruginosa respectively. The study identified that Aloe vera gel possesses antibacterial activity against MDR S. aureus and P. aeruginosa isolated from wound infection. Keywords: Aloe vera gel, Staphylococcus aureus, Pseudomonas aeruginosa, antibacterial, MDR

scholarly journals Presence of different beta-lactamase classes among clinical isolates of Pseudomonas aeruginosa expressing AmpC beta-lactamase enzyme

2010 ◽  
Vol 4 (04) ◽  
pp. 239-242 ◽  
Author(s):  
Supriya Upadhyay ◽  
Malay Ranjan Sen ◽  
Amitabha Bhattacharjee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Treatment Options ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Disk Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Enzyme Detection

Introduction: Infections caused by Pseudomonas aeruginosa are difficult to treat as the majority of isolates exhibit varying degrees of beta-lactamase mediated resistance to most of the beta-lactam antibiotics. It is also not unusual to find a single isolate that expresses multiple β-lactamase enzymes, further complicating the treatment options. Thus the present study was designed to investigate the coexistence of different beta-lactamase enzymes in clinical isolates of P. aeruginosa. Methodology: A total of 202 clinical isolates of P. aeruginosa were tested for the presence of AmpC beta-lactamase, extended spectrum beta-lactamase (ESBL) and metallo beta-lactamase (MBL) enzyme. Detection of AmpC beta-lactamase was performed by disk antagonism test and a modified three-dimensional method, whereas detection of ESBL was done by the combined disk diffusion method per Clinical and Laboratory Standards Institute (CLSI) guidelines and MBL were detected by the Imipenem EDTA disk potentiation test. Results: A total of 120 (59.4%) isolates were confirmed to be positive for AmpC beta-lactamase. Among them, 14 strains (7%) were inducible AmpC producers. Co-production of AmpC along with extended spectrum beta-lactamase and metallo beta-lactamase was reported in 3.3% and 46.6% isolates respectively. Conclusion: The study emphasizes the high prevalence of multidrug resistant P. aeruginosa producing beta-lactamase enzymes of diverse mechanisms. Thus proper antibiotic policy and measures to restrict the indiscriminative use of cephalosporins and carbapenems should be taken to minimize the emergence of this multiple beta-lactamase producing pathogens.

scholarly journals POST OPERATIVE WOUND INFECTION

2010 ◽  
Vol 17 (02) ◽  
pp. 174-179
Author(s):  
AAMIR IJAZ ◽  
SUHAIL AMER
Keyword(s):  
Inguinal Hernia ◽  
Hernia Repair ◽  
Antibiotic Prophylaxis ◽  
Wound Infection ◽  
Prophylactic Antibiotics ◽  
Postoperative Wound Infection ◽  
Wound Infections ◽  
Postoperative Wound ◽  
Lichtenstein Hernia Repair ◽  
Significant Difference

Background: The use of antibiotic prophylaxis during Lichtenstein inguinal hernia surgery is controversial, and no definitive guidelines are available in literature. Objective: To determine effects of prophylactic antibiotics in reducing the frequency of postoperative wound infection in Lichtenstein hernia repair. Study Design: Case control study. Setting: Surgical Unit II, Allied Hospital, Faisalabad. Duration: One year, between January 2007 and December 2007. Methods: Patients undergoing unilateral, primary inguinal hernia repairelectively with the Lichtenstein technique using polypropylene mesh were randomized to receive 1.0 g intravenous Cefazolin before the incision or an equal volume of placebo. Wound infection was defined according to the criteria of Centers for Disease Control and recorded. Results were assessed using chi-square test. Results: 100 patients were included in the study. Minimum age of patients in this study was 20 and maximum 75 years with a mean of 44.06 in group A and 44.84 in group B. The total number of wound infections was 7 (7%); 2 (4%) in the antibiotic prophylaxis group and 5 (10%) in the placebo group. Statistical analysis showed no significant difference in the number of wound infections in both groups (p value=0.240). Conclusions: We conclude that in Lichtenstein inguinal hernia repair routine use of prophylactic antibiotics is not needed, as it does not significantly reduce the postoperative wound infection rates.

scholarly journals A study of preoperative, intra-operative and postoperative factors responsible for postoperative wound infection

2017 ◽  
Vol 4 (5) ◽  
pp. 1569
Author(s):  
Shobha S. Nisale ◽  
Meghraj Chawada ◽  
Ganesh K. Kharkate ◽  
Sudhir B. Deshmukh
Keyword(s):  
Wound Infection ◽  
Care Center ◽  
Hospital Environment ◽  
Initial Assessment ◽  
Postoperative Wound Infection ◽  
Surgical Wound ◽  
Postoperative Wound ◽  
Health Care Center ◽  
Duration Of Surgery ◽  
Operative Findings

Background: Many factors affect the incidence of surgical wound infection, in addition to the surgeon’s skill and the hospital environment. Host attributes, such as age over 60 years, diabetes mellitus, malignant disease, obesity, malnutrition, length of preoperative stay or pre-existing infection may influence risk, as may such operation characteristics as site, urgency, duration and time of skin shaving. Objective was to study the preoperative, intra-operative and postoperative factors responsible for postoperative wound infection.Methods: This descriptive study was designed to study the problem of postoperative wound infection at tertiary health care center at rural set up over a period of two years during 2014 to 2016. Initial assessment of intra operative findings divided these cases into clean, clean contaminated and contaminated cases.Results: As the length of pre-operative stay increased, the occurrence of SSIs increased. As the duration of post operative hospital stay increased, the occurrence of SSIs also increased. The occurrence of SSI increased as the quality of surgical wound deteriorated. As the duration of surgery increased, the occurrence of SSIs increased. It was found that the order of surgery was not related to occurrence of SSIs. It was found that the rate of SSI was more (21.55%) when the drain was used in comparison to only 8.04% when the drain was not used. The most common organism found to cause SSIs was staphylococcus aureus in 33.07% of cases.Conclusions: Slightly low incidence of SSIs in our study may be attributed to the better infection control practices though it must be concluded that more stringent aseptic measures including rational antibiotic policy will be contributory in lowering the SSI rate further.

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