scholarly journals Studi Induksi Mutasi Genetik Pisang Barangan Melalui Aplikasi Iradiasi Sinar Gamma Secara In Vitro

2012 ◽  
Vol 2 (2) ◽  
pp. 97-102
Author(s):  
Zarmiyeni Zarmiyeni ◽  
Ishak Ishak
Keyword(s):  
Irradiation Dose ◽  
Gamma Ray ◽  
Shoot Tip ◽  
Shoot Bud ◽  
Dose Irradiation

       The study of mutation genetic induct Barangan banana by irradiation gamma ray according in vitro aimed to learning and evaluate dose irradiation gamma ray in to increase various of genetic planet barangan banana that regeneration of shoot tip by direction to withered ache. More 20 shoot tip each in irradiation by 10 Gy and 20 Gy, and countrat as different. Planet in to regeneration in MS media to contain 3 mg/ 1 BAP and 0,5 mg/ 1 IAA. Result accuration showed percentation bud was grow after irradiation Gy 80% Irradiation, 20 Gy 75% Irradiation and 85% countrol. Irradiation dose to affect sum propagoula produce. The increase of irradiation dose tedence to increase sum propagoula produce. The propagoula produce of this accuration was 5 sort that 15 planet, shoot, bud, bud group and nodule

scholarly journals Clonal Propagation In Vitro of Paphiopedilum Hybrids from Adult Plants

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1565-1569
Author(s):  
Vi Nguyen Tuong Do ◽  
Shan-Te Hsu ◽  
Yung-I Lee
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Seasonal Effect ◽  
Optimal Timing ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Shoot Tip Explants ◽  
Adult Plants

The aim of this study was to develop an efficient protocol for shoot tip culture from adult plants of Paphiopedilum Pfitzer. A considerable seasonal effect on explant collection was observed in the aseptic cultures established from adult plants, including the survival and microbial contamination of explants. The shoot tip explants excised from adult plants in February and May showed higher survival and had less contamination than those explants excised in August and November. Moreover, the season of explant collection also affected the subsequent shoot forming capacity and multiplication of axillary buds. In Paphiopedilum ‘In-Charm Silver Bell’, higher shoot forming capacity was observed in February and May, whereas higher shoot multiplication was observed only in February. In Paphiopedilum ‘Hsinying Maudiae Leopard’, both February and May were optimal timing for shoot forming capacity and multiplication. We also demonstrated the effectiveness of transcinnamic acid (tCA), an antiauxin chemical in diminishing the apical dominance of shoot tip explant and thus improving the axillary bud outgrowth. In P. ‘In-Charm Silver Bell’, the addition of 100 μM tCA plus 13.3 μM 6-benzylaminopurine (BA) for 1 month promoted axillary shoot bud formation from shoot tip explants as compared with the control.

scholarly journals Gamma-Ray Treatment ofEchinococcusProtoscoleces prior to Implantation in Mice Reduces Echinococcosis

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Qing Yuan ◽  
Bo Li ◽  
Shiping Jiang ◽  
Qiang Zhao ◽  
Ji Duo ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
Surgical Treatment ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Abdominal Cavity ◽  
Surgical Removal ◽  
Parasitic Disease ◽  
Hydatid Cysts ◽  
Blank Group

Echinococcosis is a serious parasitic disease caused byEchinococcustapeworms. Protoscoleces are sometimes released during surgical treatment for hydatid cysts, causing the recurrence of echinococcosis. Protoscoleces may be susceptible to radiation therapy. In this studyEchinococcusprotoscoleces were culturedin vitroand then divided into four differentγ-ray irradiation dose groups (10 Gy, 20 Gy, 40 Gy, and 80 Gy) and a blank group. The protoscoleces were then implanted into the abdominal cavity of mice. Four months later, we observed that the incidence and weight of cysts declined with the increase of irradiation dose.γ-ray irradiation can suppress the generation ofEchinococcusoriginated from protoscolex, the reason of which is due to the damaging to the structure ofEchinococcus. Irradiation may prevent echinococcosis recurrence after surgical removal of hydatid cysts.

scholarly journals Gamma-ray mutagenesis on grapevine rootstocks cultivated in vitro (1)

OENO One ◽  
1995 ◽  
Vol 29 (1) ◽  
pp. 1
Author(s):  
A. Lima da Silva ◽  
J. P. Doazan
Keyword(s):  
Leaf Area ◽  
Gamma Rays ◽  
Root Development ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Dry Weight ◽  
Shoot Development ◽  
Γ Irradiation ◽  
Varietal Susceptibility

<p style="text-align: justify;">The efficiency of γ-irradiation as a tool for breeding grapevine rootstocks was investigated. One-bud cuttings from vitroplants of two varieties (Fercal and Gravesac) were subjected to gamma rays from Cs<sup>137</sup> source at rates from 10 to 60 Grays (Gy) at intervals of 10. All the parameters observed were affected at 20 Gy and upwards. Varietal susceptibility to irradiation was different since the maximum withstanding dose was 40 Gy for Gravesac and only 30 for Fercal. Besides, shoot development was more lowered than root development. The results showed that an increase of the irradiation dose decreased the rate of survival, the rhizogenesis and the growth of<em> in vitro</em> plants. On the V<sub>2</sub> vegetative generation ofGravesac vitroplants changes in size, in leafform, in plant habits, in growth and chlorophyll deficient mutations were recorded. Moreover, after a 30 Gy irradiation variations of growth, dry weight, leaf area, number of stomata and photosynthesis rates were shown on the V<sub>3</sub> generation. Accordingly this irradiation method is efficient to develop an <em>in vitro</em> variability. A sample of the surviving plants was established in the greenhouse for further screening.</p><p style="text-align: justify;"><br />(1) Communication faite au 6'®me Symposium International sur l'Amélioration de la Vigne, à Yalta (Crimée), Ukraine, du 4 au 10 septembre 1994</p>

scholarly journals Induction of shoot buds, multiplication and plantlet formation in seedling explants of bell pepper (Capsicum annuum L.) cv. Bryza in vitro

2014 ◽  
Vol 71 (3) ◽  
pp. 187-193
Author(s):  
Andrzej Gatz
Keyword(s):  
Capsicum Annuum ◽  
Cotyledonary Node ◽  
Explant Culture ◽  
Shoot Tip ◽  
Shoot Buds ◽  
Plantlet Formation ◽  
Shoot Bud ◽  
Cotyledon Explants ◽  
Bud Induction

In vitro shoot bud induction and multiplication as well as plantlets formation from different parts of 21-d old seedlings (shoot tip, cotyledonary node, distal part of cotyledon, acropetal section of hypocotyl) of <em>Capsicum annuum</em> L., cv. Bryza were compared. During 4 weeks of primary explant culture on initiation media, first shoot bud primordia appeared; they reminded leaf primordia and subsequently some of them underwent enlargement, some developed into leaves and leaf-like structures (mainly on cotyledon explants). The highest number of shoot bud primordia was noted on cotyledonary node explants, but they were smaller than those on the remaining types of the explants. The best response of shoot regeneration showed cotyledon explants on which most of shoot buds were formed in each from four treated passages. From shoot buds on elongation media after 4 weeks of culture rooted rosettes of leaves were achieved, and the extension of the culture time to eight weeks without subculture caused that the rosettes developed into plantlets. Throughout four successive passages plantlets were obtained from cotyledon and shoot tip explants.

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

2020 ◽  
Vol 30 (1) ◽  
pp. 87-96
Author(s):  
Shabaree YA Sangma ◽  
Lolly S Pereira ◽  
JC Dang ◽  
Binu Mathew
Keyword(s):  
Leaf Disc ◽  
Shoot Length ◽  
Shoot Tip ◽  
Root Tip ◽  
Nodal Segment ◽  
Ms Medium ◽  
Shoot Bud ◽  
Shoot Number ◽  
Half Strength

Of the five explants viz., shoot tip, nodal segment, leaf disc, cotyledon and root tip excised from exegenic seedlings of Citrus indica Tanaka shoot tip cultured in MS supplemented with 0.5 mg/l of BAP generated shoots within 4.74 days, exhibiting highest percentage of response (85.82%) with highest number of shoots (8.9) and shoot length (3.04 cm). On the other hand nodal segment cultured in MS supplemented with 1.0 mg/l BAP showed 80% response in 5.16 days with a shoot number of 5.41 and shoot length of 2.43 cm. Cotyledon explants inoculated on MS supplemented with1.0 mg/l of TDZ produced shoots in 20 days with the highest response of 69.88%, with 3.77 shoots per cotyledon and shoot length of 2.03cm. Viable callus was obtained from leaf disc cultured on half strength MS medium with less Ca++ with 2, 4-D 0.5 mg/l + Kn 0.25 mg/l. This callus when inoculated on half strength MS medium with Kn 1.5 mg/l showed highest shoot bud proliferation of 66.66% with 10.06 shoots per callus. Root tip explant failed to produce any shoots. In vitro raised shoots of Citrus indica when cultured on half strength MS medium supplemented with NAA (1.0 mg/l) showed 80 % rooting in 5.66 days, with highest number of roots (6.16 per shoot) and longest root (3.78cm). Ninety per cent of in vitro rooted plantlets of Citrus indica survived in open conditions. Plant Tissue Cult. & Biotech. 30(1): 87-96, 2020 (June)

scholarly journals Optimization of gamma ray irradiation dose on strawberry plantlets

2021 ◽  
Vol 883 (1) ◽  
pp. 012018
Author(s):  
D Saptadi ◽  
H Arisah ◽  
D Agisimanto
Keyword(s):  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Changes ◽  
Lethal Dose ◽  
Dosage Level ◽  
Optimal Dose ◽  
Negative Effects ◽  
Genetic Changes ◽  
Low Genetic Diversity

Abstract As a commodity that is cultivated vegetatively, strawberries have a relatively low genetic diversity. One effort to increase the diversity of strawberries and genetic quality improvement can be made by using gamma-ray radiation. In vitro explant of strawberry cv Early Bright were radiated with Gamma-ray P3TIR BATAN facility. Starting with a radiosensitivity test to find out Lethal Dose (LD) 50 with dosage level; 5, 10, 20, 30, 40, 60, 80, 100, 125, 150, 175, and 200 Gy. LD50 observations were carried out to get the optimal dose. The treatment of several doses of gamma-ray radiation showed varied results, with the percentage of explants living decrease by increasing the dose of irradiation. Irradiation dose until 10 Gy did not affect the survival rate of explant—the treatment dose of 80 Gy and above causes 100% mortality on the final observation. The dose of 60 Gy had a significant effect on shoot growth, as indicated by curly leaves' growth. The next optimal dose recommended for radiation is set at 20 and 30 Gy to anticipate genetic changes that are not morphologically expressed and to avoid the negative effects of morphological changes.

Effects of gamma ray on some molecular characteristics of in vitro regenerated Gerbera jamesonii Bolus

1970 ◽  
Vol 39 (2) ◽  
pp. 207-214
Author(s):  
Nazma Akter ◽  
RH Sarker ◽  
MI Hoque
Keyword(s):  
Gamma Ray ◽  
Molecular Characteristics ◽  
Gerbera Jamesonii

DOI: 10.3329/bjb.v39i2.7490Bangladesh J. Bot. 39(2): 207-214, 2010 (December)

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

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