scholarly journals Mouse thymic mast cells in normal state and after stress-induced atrophy

2013 ◽  
Vol 10 (4) ◽  
pp. 24-32
Author(s):  
V V Guselnicova ◽  
A V Polevschikov
Keyword(s):  
Mast Cells ◽  
Alcian Blue ◽  
Toluidine Blue ◽  
Normal State ◽  
Polyclonal Antibodies ◽  
Adult Mice ◽  
Embryonic Life ◽  
Blue Stain ◽  
Outbred Mice ◽  
Adult Thymus

Background. Studying of thymic mast cells population in normal state and after stressinduced atrophy. Methods. The study was performed on 80 thymus of white outbred mice with using of histochemistry and immunohistochemistry methods. Sections of embryonic thymus were stained with toluidine blue. Adult mice were given a single injection of 2,5 mg of hydrocortisone for induction of thymic accidental transformation; sections were stained with toluidine blue and alcian blue-safranin. Within of immunohistochemical research paraffin sections of adult thymus were stained with polyclonal antibodies to synaptophysin and tyrosine hydroxylase with alcian blue stain. Results. Mast cells (MCs) appeared in thymus on 19th day of embryonic life and demonstrated mainly a medullar location. In adult animals MCs were observed only in the connective tissue of the capsule, interlobular septa, subcortex and perivascular space. Mast cells matured in thymus after accidental transformation. The localization of developing mast cells was changing from medullar and cortical to capsular. A morphological proximity between nerve terminals and mast cells has been observed in normal adult thymus. Some of these nerves were catecholaminergic. Conclusion. Possible important role of thymic mast cells and mast cellsnerves interaction in normal state and after accidental transformation is discussed.

scholarly journals Abnormal mast cell granules in the beige (Chédiak-Higashi syndrome) mouse.

1975 ◽  
Vol 23 (2) ◽  
pp. 117-122 ◽  
Author(s):  
E Y Chi ◽  
D Lagunoff
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Toluidine Blue ◽  
Ruthenium Red ◽  
Chediak Higashi Syndrome

Mast cells of beige (C57BL/6J) (bg-j/bg-j) mice were examined histochemically and ultrastructurally. Mast cell granules in the beige mice were markedly enlarged and irregular in shape. Granule contents stained uniformly with acidified toluidine blue, but with ruthenium red and Alcian Blue-safranin, two components were evident. The rims of the abnormal granules stained with ruthenium red and with Alcian Blue; the centers of the granules were clear with ruthenium red and stained with safranin. Mast cell granules thus represent another abnormal organelle in the Chédiak-Higashi syndrome.

The Tinctorial Behaviour of Human Mast Cells

1954 ◽  
Vol s3-95 (29) ◽  
pp. 1-4 ◽  
Author(s):  
WILLIAM MONTAGNA ◽  
ARTHUR Z. EISEN ◽  
ALLEN S. GOLDMAN
Keyword(s):  
Mast Cells ◽  
Blood Vessels ◽  
Toluidine Blue ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Running Water ◽  
Enzyme Preparations ◽  
Reactive Substance ◽  
Ph Values ◽  
Blue Stain

Mast cells in the skin differentiate from perivascular fibroblasts. The cells nearest the walls of the blood-vessels contain mostly sparse and small mast granules; in those farther removed from the blood-vessels the granules are more numerous and coarse. With weak solutions of toluidine blue, mast granules reveal maximal chromotropy at pH 5-0. At lower pH values not all of the granules stain; at higher ones the granules and the intergranular cytoplasm stain progressively more orthochromatically. After digestion with ribonuclease and staining with toluidine blue buffered to pH 4.0 or 5.0 the mast granules are cherry red and all traces of orthochromatic staining are abolished; when stained at pH 60 or above, however, the cytoplasm and the granules attain a strong blue stain as if they had not been digested in the enzyme. Preparations fixed in Helly's fluid may be washed in running water overnight and the mast granules show no diminution in chromotropy. The same sections may be stained, destained, and stained again at any desired pH with excellent results. Both the cytoplasm and the granules are Schiff-reactive, but the granules stain more intensely than the background. Sections stained with the periodic acid/Schiff technique and subsequently stained with toluidine blue reveal the mast granules brilliantly metachromatic, suggesting that the metachromatic and the Schiff-reactive substances, although coexistent, may be in fact separate elements. Mast granules, according to these tinctorial reactions, then, may contain 4 substances: (a) a protein cytoskeleton stainable with toluidine blue buffered to pH 6.0 or above; (b) some ribonucleic acid removable with ribonuclease and stainable with toluidine blue buffered to pH 5.0 or below; (c) an acid mucopolysaccharide which stains metachromatically; and (d) a Schiff-reactive substance.

scholarly journals Diffuse Cutaneous Mastocytosis (Pigmented Maculopapular Cutaneous Mastocytosis) in a Cat

2020 ◽  
Vol 43 (1) ◽  
pp. 81-83
Author(s):  
Marcin Szczepanik ◽  
Piotr Wilkołek ◽  
Anna Śmiech ◽  
Iwona Taszkun
Keyword(s):  
Mast Cells ◽  
Toluidine Blue ◽  
Hair Follicles ◽  
Central Nuclei ◽  
Granular Cytoplasm ◽  
Blue Stain ◽  
Superficial Dermis ◽  
Well Differentiated ◽  
Metachromatic Granules ◽  
Cutaneous Mastocytosis

AbstractDiffuse cutaneous mastocytosis was diagnosed in a 6-year-old, indoor, neutered female domestic European shorthair cat. Marked pruritus located mainly on the head and neck was noticed in the cat and in this area the animal had developed alopecia, crusts, and plaques. Histologically, monomorphic mast cells were found in the superficial dermis and around the hair follicles. Mast cells were well differentiated, with central nuclei and granular cytoplasm, with metachromatic granules which stained positively with Toluidine blue stain. The animal was successfully treated with oclacitinib at a dose of 1 mg/kg, twice a day per os.

scholarly journals SIGNIFICANCE OF MAST CELLS IN BENIGN AND MALIGNANT CONDITIONS OF PROSTATE

2021 ◽  
pp. 154-155
Author(s):  
Hemanth Kumar ◽  
R M Potekar ◽  
Satish Arakeri
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Toluidine Blue ◽  
High Power ◽  
P Value ◽  
Benign Lesions ◽  
Special Stain ◽  
Blue Stain ◽  
The One ◽  
Malignant Condition

Introduction: Mast cells are the one of the component of defense immunity of our body.Aims of the study:Role of mast cell in benign and malignant condition of prostate.Materials & methods:Total number of cases is 32.Out of 32 cases,24 cases are Benign (BPH) and 8 cases are malignant. Special stain ie.Toluidine blue has been done in all the 32 cases, as toluidine blue stain will highlight the mast cells accurately.The number of mast cells per 10 high power fields in both benign and malignant conditions was compared using statistical tools.Results: Proportion of mast cell in benign lesions is 0.58 while in malignancy is 0.63.Since the p-value was > 0.05,the results were considered as statistically insignificant. Discussion: In the present study, it is found that there is no statistical significant increase in the number of mast cells. Conclusion: Hence probably mast cells may not play any protumorogenic role in cases of prostatic malignancy.

scholarly journals Trisomy 19 and T(9;22) In a Patient with Acute Basophilic Leukemia

2011 ◽  
Vol 2011 ◽  
pp. 1-3 ◽  
Author(s):  
Alicia Rojas-Atencio ◽  
Karelis Urdaneta ◽  
Marisol Soto-Quintana ◽  
Francisco Alvarez Nava ◽  
Jenny Cañizales ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Mast Cells ◽  
Positive Reaction ◽  
Toluidine Blue ◽  
Ultrastructure Study ◽  
Molecular Cytogenetic ◽  
Cytogenetic Studies ◽  
Blue Stain ◽  
Basophilic Leukemia ◽  
Clonal Abnormalities

We report a case of acute basophilic leukemia with two coexisting clonal abnormalities, t(9;22) and trisomy 19. The blast showed positive reaction with myeloperoxidase but negative reaction with chloroacetate esterase and acid phosphatase. Metachromatic features of the blast were observed with toluidine blue stain. Ultrastructure study showed the presence of azurophilic granules in basophils and blast mast cells. Conventional and molecular cytogenetic studies revealed, t(9;22) withBCR/ABLpositive and trisomy 19 in all metaphase cells. To our knowledge, this paper here is the first to present acute basophilic leukemia with trisomy 19 and t(9;22).

scholarly journals HISTOCHEMICAL COMPARISON OF FROG AND RAT MAST CELLS

1971 ◽  
Vol 19 (6) ◽  
pp. 369-375 ◽  
Author(s):  
HERBERT CHIU ◽  
DAVID LAGUNOFF
Keyword(s):  
Acid Phosphatase ◽  
Mast Cells ◽  
Phosphatase Activity ◽  
Alcian Blue ◽  
Toluidine Blue ◽  
Acid Phosphatase Activity ◽  
Proteolytic Enzymes ◽  
Nonspecific Esterase ◽  
Esterase Activities ◽  
Rat Mast Cells

Histochemical procedures were used to compare the constituents of frog and rat mast cells. On the basis of staining with acidified toluidine blue and the Alcian Blue-safranin combination, a substance characterized as heparin in rat cells was identified in frog mast cells. In contrast to their presence in rat cells, no histamine, serotonin or catecholamines could be observed in frog mast cells with the o-phthalaldehyde and paraformaldehyde reactions. A search for activities of two proteolytic enzymes, chymotrypsin-like and trypsin-like esterases, in frog mast cells was unsuccessful. β-Glucuronidase, N-acetyl-β-d-glucosaminidase and nonspecific esterase activities were demonstrated in both frog and rat mast cells; acid phosphatase activity was detected in frog but not rat mast cells.

Mast Cell Heterogeneity in Human Lung Tissue

1989 ◽  
Vol 77 (3) ◽  
pp. 297-304 ◽  
Author(s):  
F. J. Van Overveld ◽  
L. A. M. J. Houben ◽  
F. E. M. Schmitz du Moulin ◽  
P. L. B. Bruijnzeel ◽  
J. A. M. Raaijmakers ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Lung Tissue ◽  
Human Lung ◽  
Immunoglobulin E ◽  
Prostaglandin D2 ◽  
Leukotriene C4 ◽  
Human Lung Tissue ◽  
No Release

1. In this study mast cells were found to comprise 2.1% of total cells recovered by enzymatic digestion of human lung tissue. 2. This mast cell population consisted of 79% formalin-sensitive, Alcian Blue-positive mast cells and 21% formalin-insensitive, Alcian Blue-positive mast cells. 3. By the use of centrifugal elutriation and subsequent Percoll gradient centrifugation, separate mixed cell populations could be obtained in which the mast cell constituents were either of the formalin-sensitive or -insensitive type. 4. Cell suspensions in which formalin-sensitive cells comprised 97% of mast cells contained approximately 1.34 pg of histamine per mast cell, whereas in preparations in which mast cells were 84% formalin-resistant the histamine content was approximately 4.17 pg of histamine per mast cell. 5. The histamine release upon anti-immunoglobulin E challenge of formalin-sensitive mast cells was greater than the release by formalin-insensitive mast cells. 6. After challenge with opsonized zymosan, only formalin-sensitive mast cells were able to release histamine. 7. Leukotriene C4 release was observed when formalin-sensitive mast cells were challenged with antiimmunoglobulin E. Formalin-insensitive mast cells showed no release of leukotriene C4. 8. Prostaglandin D2 release was observed when formalin-insensitive mast cells were challenged with antiimmunoglobulin E. Formalin-sensitive mast cells showed no release of prostaglandin D2.

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