scholarly journals The effect of XIVb strain of Newcastle disease virus on the haematology of Muscovy ducks and local chickens

2020 ◽  
pp. 28-35
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Lethal Dose ◽  
Haematological Parameters ◽  
Veterinary Research ◽  
Muscovy Ducks ◽  
Hb Concentration ◽  
And Control ◽  
Local Chickens

Introduction: Anseriform species such as Muscovy ducks, Mallard ducks and geese are commonly known to be susceptible to some of Newcastle disease virus (NDV) strains, while resistant to others. This study was designed to determine and compare the effect of XIVb strain of NDV on the haematological parameters of Muscovy ducks in relation to the local chickens. Methods: Forty experimental birds consisting of twenty Muscovy ducklings and twenty local chicks at five weeks of age were divided into four groups of 10 subjects each, designated as infected chickens (IC), control chickens (CC), infected ducklings (ID) and control ducklings (CD) and were inoculated orally with 107.8/ 0.1ml /bird as the embryo lethal dose (ELD50/ml) of the strain obtained from the National Veterinary Research Institute, Vom. The means of haematological parameters were used to determine the response of the birds in each group and were compared to their controls (at days 0, 3, 7, 10, 14, 21, 28, and 35). Results: The result showed reduction in some of the haematological parameters, such as RBC and PCV, MCV, HB concentration, MCH and leukocyte count of both the IC and ID. RBC was (1.71±0.26×1012/l) and (2.77±0.20×1012/l) in the IC and ID against their control groups (3.69±0.31×1012/l) and (3.71±0.31×1012/l) on day 7PI, respectively. The IC also showed significant (p < 0.05) lymphopenia on days 7, 10 and 21 than the CC while the ID showed significant (p < 0.05) lymphocytosis on days 14, 21, 28 and 35 than the CD. Significance: The haematological parameters of the local chickens are more affected when infected with XIVb strain of NDV than that of the Muscovy ducks.

scholarly journals Clinical and gross-pathological changes in Muscovy ducks and Nigerian local chickens infected with Newcastle disease virus (XIVb strain)

2021 ◽  
Vol 18 (4) ◽  
pp. 182-190
Author(s):  
S.G. Usman ◽  
S.B. Oladele ◽  
L. Saidu ◽  
M.S. Muhammed ◽  
F.S. Umar ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Pathological Changes ◽  
Muscovy Ducks ◽  
Local Chickens

No Abstract.

scholarly journals Preparation and bioactivity of anti-Newcastle disease virus-phosphoprotein cytoplasmic transduction peptide antibody

2020 ◽  
Author(s):  
Benqiang Li ◽  
Man Wang ◽  
Jianguo Zhu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Protein Function ◽  
Cell Protection ◽  
Test Analysis ◽  
Hiv Tat ◽  
Significant Difference ◽  
Macromolecular Protein ◽  
And Control

Abstract On the basis of cell-penetrating peptide’s character that it can penetrate cytomembrane and transfer macromolecular protein to cytoplasm so to play biological function, we took the experiments. The fuse penetrating peptide our experiment adoptted is HIV-TAT derived fragment-CTP512, with good transmember effect and distinct cytoplasm-position. In this chapter, the research of transmembrane character was processed first. According to the tests on trans- member protein with different concentrations, the best trans-member concentration is 3µM. Afterwards, we found that the location of trans-member antibody is overlapping with phosphoprotein using indirect immunofluorescence test analysis. According to MTT test, there is no significant difference between CTP fusion protein and control on cell proliferation and viability. TCID50 test was used to detect the protective effect of trans-member antibody on cell. Result showed that trans- member antibody has significant cell protection effect compared to the control in the order: ZL.103>ZL.17>Control. Fluorogenic quantitative PCR result showed that trans- member antibody can disturb the duplication and transcription of Newcastle disease virus. This results not only paved a good way to research the transport of disease related protein, but also provide a splendid tool on protein function research.

scholarly journals Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination

2020 ◽  
Vol 45 (2) ◽  
pp. 78-90
Author(s):  
R.W. Astuti ◽  
N. Wijayanti ◽  
A. Haryanto
Keyword(s):  
Antibody Response ◽  
Fusion Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Fusion Protein ◽  
Blue Staining ◽  
F Protein ◽  
Local Isolate ◽  
And Control

This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines.

scholarly journals Development of a Recombinant Thermostable Newcastle Disease Virus (NDV) Vaccine Express Infectious Bronchitis Virus (IBV) Multiple Epitopes for Protecting against IBV and NDV Challenges

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 564
Author(s):  
Lei Tan ◽  
Guoyuan Wen ◽  
Yanmei Yuan ◽  
Meizhen Huang ◽  
Yingjie Sun ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Infectious Bronchitis Virus ◽  
Newcastle Disease ◽  
Rural Areas ◽  
Lethal Dose ◽  
Morphological Characteristics ◽  
Ciliary Activity ◽  
Virus Challenge ◽  
Infectious Bronchitis

Newcastle disease (ND) and infectious bronchitis (IB) are two highly contagious diseases that severely threaten the poultry industry. The goal of this study is to prevent these two diseases and reduce the vaccine costs during storage and transportation. In this study, we design a thermostable recombinant Newcastle disease virus (NDV) candidate live vaccine strain designated as rLS-T-HN-T/B, which expresses the multiple epitope cassette of the identified infectious bronchitis virus (IBV) (S-T/B). The rLS-T-HN-T/B strain was found to possess similar growth kinetics, passage stability, morphological characteristics, and virulence to the parental LaSota strain. After incubation at 56 °C at the indicated time points, the rLS-T-HN-T/B strain was determined by the hemagglutination (HA), and 50% embryo infectious dose (EID50) assays demonstrated that it accords with the criteria for thermostability. The thermostable rLS-T-HN-T/B and parental LaSota vaccines were stored at 25 °C for 16 days prior to immunizing the one-day-old specific pathogen-free (SPF) chicks. Three weeks postimmunization, the virus challenge results suggested that the chicks vaccinated with the rLS-T-HN-T/B vaccine were protected by 100% and 90% against a lethal dose of NDV and IBV, respectively. Furthermore, the trachea ciliary activity assay indicated that the mean ciliostasis score of the chicks vaccinated with thermostable rLS-T-HN-T/B vaccine was significantly superior to that of the LaSota and PBS groups (p < 0.05). The rLS-T-HN-T/B vaccine stored at 25 °C for 16 days remained capable of eliciting the immune responses and protecting against IBV and NDV challenges. However, the same storage conditions had a great impact on the parental LaSota strain vaccinated chicks, and the NDV challenge protection ratio was only 20%. We conclude that the thermostable rLS-T-HN-T/B strain is a hopeful bivalent candidate vaccine to control both IB and ND and provides an alternative strategy for the development of cost-effective vaccines for village chickens, especially in the rural areas of developing countries.

scholarly journals Muscovy ducks infected with velogenic Newcastle disease virus (genotype VIId) act as carriers to infect in-contact chickens

2019 ◽  
Vol 98 (10) ◽  
pp. 4441-4448 ◽  
Author(s):  
Ahmed R Elbestawy ◽  
Hany F Ellakany ◽  
Hatem S Abd El-Hamid ◽  
Rasha E Zedan ◽  
Ahmed R Gado ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Genotype ◽  
Velogenic Newcastle Disease Virus ◽  
Muscovy Ducks

scholarly journals Serological Survey of Newcastle Disease in Free Ranging Local Chickens in the Federal Capital Territory, Abuja, Nigeria

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Samuel Akawu Anzaku ◽  
Jariath Umoh Umoh ◽  
Paul Ayuba Abdu ◽  
Junaidu Kabir ◽  
Akawu Bala
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Serological Survey ◽  
History Of ◽  
Commercial Poultry ◽  
Free Ranging ◽  
Local Chickens ◽  
Apparently Healthy

A serological survey was carried out in four area councils (Abuja Municipal, Kuje, Gwagwalada, and Kwali) of the Federal Capital Territory (Abuja) to determine the prevalence of antibodies to Newcastle disease virus in local chickens using haemagglutination inhibition (HI) tests. In each area council, one hundred sera samples were collected from apparently healthy local chickens with no history of vaccination. Abuja Municipal, Kuje, Gwagwalada, and Kwali area councils had prevalence of 37, 44, 79, and 68%, respectively. The overall prevalence of antibody to Newcastle disease in the four area councils was 57%. This study shows that Newcastle disease virus is circulating in local chickens in the study area, and this may pose a serious threat to the commercial poultry industry within these four area councils of the Federal Capital Territory (Abuja) where this study was carried out.

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