scholarly journals Preparation and bioactivity of anti-Newcastle disease virus-phosphoprotein cytoplasmic transduction peptide antibody

2020 ◽  
Author(s):  
Benqiang Li ◽  
Man Wang ◽  
Jianguo Zhu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Protein Function ◽  
Cell Protection ◽  
Test Analysis ◽  
Hiv Tat ◽  
Significant Difference ◽  
Macromolecular Protein ◽  
And Control

Abstract On the basis of cell-penetrating peptide’s character that it can penetrate cytomembrane and transfer macromolecular protein to cytoplasm so to play biological function, we took the experiments. The fuse penetrating peptide our experiment adoptted is HIV-TAT derived fragment-CTP512, with good transmember effect and distinct cytoplasm-position. In this chapter, the research of transmembrane character was processed first. According to the tests on trans- member protein with different concentrations, the best trans-member concentration is 3µM. Afterwards, we found that the location of trans-member antibody is overlapping with phosphoprotein using indirect immunofluorescence test analysis. According to MTT test, there is no significant difference between CTP fusion protein and control on cell proliferation and viability. TCID50 test was used to detect the protective effect of trans-member antibody on cell. Result showed that trans- member antibody has significant cell protection effect compared to the control in the order: ZL.103>ZL.17>Control. Fluorogenic quantitative PCR result showed that trans- member antibody can disturb the duplication and transcription of Newcastle disease virus. This results not only paved a good way to research the transport of disease related protein, but also provide a splendid tool on protein function research.

scholarly journals Preparation and bioactivity of anti-Newcastle disease virus-phosphoprotein cytoplasmic transduction peptide antibody

2020 ◽  
Author(s):  
Benqiang Li ◽  
Man Wang ◽  
Jianguo Zhu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Protein Function ◽  
Cell Protection ◽  
Test Analysis ◽  
Hiv Tat ◽  
Significant Difference ◽  
Macromolecular Protein ◽  
And Control

Abstract On the basis of cell-penetrating peptide’s character that it can penetrate cytomembrane and transfer macromolecular protein to cytoplasm so to play biological function, we took the experiments. The fuse penetrating peptide our experiment adoptted is HIV-TAT derived fragment-CTP512, with good transmember effect and distinct cytoplasm-position. In this chapter, the research of transmembrane character was processed first. According to the tests on trans- member protein with different concentrations, the best trans-member concentration is 3µM. Afterwards, we found that the location of trans-member antibody is overlapping with phosphoprotein using indirect immunofluorescence test analysis. According to MTT test, there is no significant difference between CTP fusion protein and control on cell proliferation and viability. TCID50 test was used to detect the protective effect of trans-member antibody on cell. Result showed that trans- member antibody has significant cell protection effect compared to the control in the order: ZL.103>ZL.17>Control. Fluorogenic quantitative PCR result showed that trans- member antibody can disturb the duplication and transcription of Newcastle disease virus. This results not only paved a good way to research the transport of disease related protein, but also provide a splendid tool on protein function research.

scholarly journals Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination

2020 ◽  
Vol 45 (2) ◽  
pp. 78-90
Author(s):  
R.W. Astuti ◽  
N. Wijayanti ◽  
A. Haryanto
Keyword(s):  
Antibody Response ◽  
Fusion Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Fusion Protein ◽  
Blue Staining ◽  
F Protein ◽  
Local Isolate ◽  
And Control

This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines.

scholarly journals Newcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickens

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1985
Author(s):  
Madhan Mohan Chellappa ◽  
Sohini Dey ◽  
Dinesh Chandra Pathak ◽  
Asmita Singh ◽  
Narayan Ramamurthy ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Virus ◽  
Vaccine Candidate ◽  
Transcriptional Unit ◽  
Slight Reduction ◽  
Bivalent Vaccine ◽  
Mouth Disease Virus ◽  
Significant Difference

Newcastle disease virus (NDV) strain R2B, with an altered fusion protein cleavage site, was used as a viral vector to deliver the immunogenic genes VP2 and VP1 of chicken infectious anaemia virus (CIAV) to generate a bivalent vaccine candidate against these diseases in chickens. The immunogenic genes of CIAV were expressed as a single transcriptional unit from the NDV backbone and the two CIA viral proteins were obtained as separate entities using a self-cleaving foot-and-mouth disease virus 2A protease sequence between them. The recombinant virus (rR2B-FPCS-CAV) had similar growth kinetics as that of the parent recombinant virus (rR2B-FPCS) in vitro with similar pathogenicity characteristics. The bivalent vaccine candidate when given in specific pathogen-free chickens as primary and booster doses was able to elicit robust humoral and cell-mediated immune (CMI) responses obtained in a vaccination study that was conducted over a period of 15 weeks. In an NDV and CIAV ELISA trial, there was a significant difference in the titres of antibody between vaccinated and control groups which showed slight reduction in antibody titre by 56 days of age. Hence, a second booster was administered and the antibody titres were maintained until 84 days of age. Similar trends were noticed in CMI response carried out by lymphocyte transformation test, CD4+ and CD8+ response by flow cytometry analysis and response of real time PCR analysis of cytokine genes. Birds were challenged with virulent NDV and CIAV at 84 days and there was significant reduction in the NDV shed on the 2nd and 4th days post challenge in vaccinated birds as compared to unvaccinated controls. Haematological parameters comprising PCV, TLC, PLC and PHC were estimated in birds that were challenged with CIAV that indicated a significant reduction in the blood parameters of controls. Our findings support the development and assessment of a bivalent vaccine candidate against NDV and CIAV in chickens.

Pathogenesis of Newcastle Disease Virus Genotype VII in Chickens Vaccinated with LaSota and Inactivated Newcastle Disease Vaccines

2020 ◽  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Bursa Of Fabricius ◽  
Virus Genotype ◽  
Challenge Virus ◽  
Significant Difference ◽  
Group D ◽  
Genotype Vii

Newcastle disease (ND) is one of the most serious viral diseases affecting poultry farms in different countries. Many outbreaks -even in vaccinated poultry flocks- were recorded in the last few years caused by Newcastle disease virus (NDV) genotype VII. This study was conducted to compare the pathogenesis of NDV genotype VII in non-vaccinated chickens and chickens vaccinated with NDV genotype II live (LaSota) and inactivated vaccines. One hundred 1-day-old chicks were divided into four equal groups; 25 for each. Groups A and B were kept unvaccinated. Group C was vaccinated with LaSota, and group D was vaccinated with both LaSota and inactivated NDV vaccine. Group A was kept as nonchallenged control blank group, while groups B, C and D were challenged intranasally by 0.1 ml 106 EID50 NDV genotype VII at 25-day of age. Three chickens were sacrificed from each group at 2, 5- and 10-days post challenge (dpc). Tissue specimens from trachea, lungs, bursa of fabricius, spleen and thymus were collected for histopathology and immunohistochemistry. NDV genotype VII challenge virus did not induce mortality in both vaccinated groups. Both vaccination programs resulted also in less severe clinical signs and histopathological lesions comparing to non-vaccinated challenged birds. Tracheal lesion score was significantly low in group D at 10 dpc while no significant difference was recorded between groups C and D in lungs. All lymphoid organs showed significantly less severe pathological alterations and depletion in groups C and D comparing to group B. Our results indicated that mis-matched genotype NDV vaccines could alleviate the pathological effect of the NDV challenge virus but do not provide complete protection of the infected host organs.

scholarly journals Efficacy of a genotype 2 Newcastle disease vaccine (Avinew®) against challenge with highly virulent genotypes 5d and 3d

2009 ◽  
Vol 80 (3) ◽  
Author(s):  
D.G. Bwala ◽  
C. Abolnik ◽  
A. Van Wyk ◽  
E. Cornelius ◽  
S.P.R. Bisschop
Keyword(s):  
South Africa ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Disease Outbreaks ◽  
Challenge Virus ◽  
Genotype 2 ◽  
Significant Difference ◽  
Specific Pathogen ◽  
Newcastle Disease Vaccine

Since 2002, following its introduction, the lineage 5d Newcastle disease virus (so-called Goose paramyxovirus -GPMV) strain has caused numerous disease outbreaks among commercial and backyard poultry in South Africa, raising questions about the ability of commercially available Newcastle disease vaccines to fully protect poultry against the strain. This study aimed to determine whether there are differences in the level of protection offered by Avinew® Newcastle disease vaccine against GPMV virus as compared with a 3d Newcastle disease virus isolated in South Africa in 1993 (Rainbow challenge virus - RCV) strain. Six groups of 10-day-old, specific pathogen-free chickens were vaccinated with doses of 103.0, 104.5 and 106.0 EID50 of Avinew® vaccine and challenged at 4 weeks of age intramuscularly at a dose of 105.3EID50/ 0.2 mℓ/bird of GPMV and RCV. No statistically significant difference could be found in the protection offered by Avinew® vaccine against GPMV as compared to RCV challenge. The protection offered against the ND challenge was found to be dose dependent. At the recommended field dose of 106.0 EID50 the vaccine gave 100 % protection from mortality against both the challenge viruses, but not against infection and replication of the viruses, as gross lesions were evident even in apparently healthy birds that survived the challenge. The protective dose (PD90) of the Avinew® vaccine against GPMV challenge was calculated at 104.38 and against that of RCV at 104.43.

scholarly journals The effect of XIVb strain of Newcastle disease virus on the haematology of Muscovy ducks and local chickens

2020 ◽  
pp. 28-35
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Lethal Dose ◽  
Haematological Parameters ◽  
Veterinary Research ◽  
Muscovy Ducks ◽  
Hb Concentration ◽  
And Control ◽  
Local Chickens

Introduction: Anseriform species such as Muscovy ducks, Mallard ducks and geese are commonly known to be susceptible to some of Newcastle disease virus (NDV) strains, while resistant to others. This study was designed to determine and compare the effect of XIVb strain of NDV on the haematological parameters of Muscovy ducks in relation to the local chickens. Methods: Forty experimental birds consisting of twenty Muscovy ducklings and twenty local chicks at five weeks of age were divided into four groups of 10 subjects each, designated as infected chickens (IC), control chickens (CC), infected ducklings (ID) and control ducklings (CD) and were inoculated orally with 107.8/ 0.1ml /bird as the embryo lethal dose (ELD50/ml) of the strain obtained from the National Veterinary Research Institute, Vom. The means of haematological parameters were used to determine the response of the birds in each group and were compared to their controls (at days 0, 3, 7, 10, 14, 21, 28, and 35). Results: The result showed reduction in some of the haematological parameters, such as RBC and PCV, MCV, HB concentration, MCH and leukocyte count of both the IC and ID. RBC was (1.71±0.26×1012/l) and (2.77±0.20×1012/l) in the IC and ID against their control groups (3.69±0.31×1012/l) and (3.71±0.31×1012/l) on day 7PI, respectively. The IC also showed significant (p < 0.05) lymphopenia on days 7, 10 and 21 than the CC while the ID showed significant (p < 0.05) lymphocytosis on days 14, 21, 28 and 35 than the CD. Significance: The haematological parameters of the local chickens are more affected when infected with XIVb strain of NDV than that of the Muscovy ducks.

scholarly journals Scoring System for Lesions Induced by Different Strains of Newcastle Disease Virus in Chicken

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Elawad A. Hussein ◽  
M. Hair-Bejo ◽  
Lawan Adamu ◽  
A. R. Omar ◽  
Siti S. Arshad ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Scoring System ◽  
Control Group ◽  
Control Groups ◽  
Specific Pathogen ◽  
Group 5 ◽  
Different Strains ◽  
And Control

Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 6 and 24 specific pathogen-free (SPF) chickens, respectively. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. In infected groups, infection was induced by intranasal administration of 10550% EID50/0.1 mL of velogenic Newcastle disease virus strain (vNDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 3, 6, and 7 postinoculation (pi). Infected chickens in experiment 2 were euthanised at hours (hrs) 2, 4, 6, 12 and days 1, 2, 4, and 6 pi. Chickens of the control group in experiment 1 were euthanised on days 3 and 7 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4, and 6 pi. Then in experiment 3, 15 SPF chickens were divided into three groups; in the first group, 5 SPF chickens were infected with vNDV, in the second group, 5 SPF chickens were infected with lentogenic NDV (lNDV) (103.0EID50/0.1 mL), and the third group was kept without infection as a control group. Chickens were euthanised on day 5 pi. In all previous experiments, tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining was applied. Tissues were examined under light microscope and changes were recorded. A scoring system was designed for lesions induced by different strains of NDV and, accordingly, lesions were scored. The scoring system was found helpful in the evaluation of disease severity.

Newcastle disease virus antibodies in apparently healthy commercial layers in Abeokuta, Ogun state, Nigeria

2020 ◽  
Vol 45 (3) ◽  
Author(s):  
F. O. Olufemi ◽  
O. Olatunji ◽  
E. O. Omoshaba
Keyword(s):  
Local Government ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Inhibition Assay ◽  
Ogun State ◽  
Poultry Birds ◽  
Significant Difference ◽  
Commercial Poultry ◽  
Apparently Healthy

Several authors have worked on Newcastle Disease (ND) with respect to the incidence, prevalence and epizootiology of the disease and the antibody status in non-vaccinated birds in Nigeria. However, current information on the antibodies of the Newcastle Disease virus in apparently healthy commercial poultry birds with known vaccination records in Abeokuta Metropolis is scanty. This study was conducted, using Heamagglutination Inhibition assay (HI) technique, to detect Newcastle Disease virus (NDV) antibodies in routinely vaccinated commercial poultry birds in the 3 Local Government Areas (LGAs) of Abeokuta that form the Abeokuta metropolis. Haemagglutinating NDV antibodies were detected in the apparently healthy layers thus indicating a widely circulating NDV in areas. A potency test of the vaccines used on the farms was determined using Heamagglutination test and their values ranged between 2 and 2 . The sero-prevalence of NDVantibodies in the 3 LGAs showed there was no significant difference (p >0.05). Of the 120 sera tested, 82 (68.33%) had detectable NDV antibodies but only 81.67% had HI protective titre of > 2 and 18.33% had low seroconversionwith titre 2 or less.

scholarly journals Evaluation of the Efficacy of Baby Chick Ranikhet Disease Vaccine and Bangla Baby Chick Ranikhet Disease Vaccine in Fayoumi Chicks

1970 ◽  
Vol 23 (2) ◽  
pp. 125-129
Author(s):  
Zafar Ahmed Bhuiyan ◽  
Paritosh Kumar Biswas ◽  
M Nural Anwar ◽  
Abdul Ahad ◽  
Nitish Chandra Debnath
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Survival Rates ◽  
Haemagglutination Inhibition ◽  
Live Vaccines ◽  
Body Weights ◽  
Similar Survival ◽  
And Control ◽  
Better Than

A study was undertaken for testing the comparative efficacy of two live vaccines produced in Bangladesh to control Newcastle disease (ND) in chickens. One of these vaccines named ‘baby chicks Ranikhet disease vaccine (BCRDV), a government vaccine produced by the Livestock Research Institute, Mohakhali, Dhaka, and the other named ‘Bangla-BCRDV®', a commercial vaccine produced recently by a pharmaceutical company. Both the vaccines are prepared using the ‘F' strain of Newcastle disease virus (NDV). Three hundred Fayoumi chicks distributed in 3 groups were used in this study. The results of this study revealed that, at the age of 49 days the survival rates of chicks belonged to BCRDV, Bangla-BCRDV® and control groups were 69, 11 and 18%, respectively. The survival rate of Fayoumi chicks in the BCRDV group was significantly higher than that of the two other groups (p <0.05). Conversely, almost similar survival rates of chicks were recorded in the control and Bangla-BCRDV® groups (p >0.05). At day-1, the haemagglutination inhibition (HI) titre to NDV of the chicks was log2 5.8 ± 0.79 (SEM). At day 35 the same HI titre was observed in the chicks of the BCRDV group, which was, 1 and 1.5 log (base 2) higher than the chicks of the control and Bangla-BCRDV® group, respectively. At day-49, HI titres to NDV ≥ log212 were recorded in the chicks of all the three groups that survived having challenged with velogenic NDV. There were no significant differences in weekly mean body-weights of the chicks in all the three groups until the week 8 when the mean bodyweight of the chicks was higher in the BCRDV group compared with the two others. These results indicted that the efficacy of BCRDV in Fayoumi chicks against ND was better than the Bangla-BCRDV®.Keywords: Baby chick ranikhet disease vaccine, Bangla baby chick ranikhet disease vaccine, Newcastle disease (ND), Newcastle disease virus (NDV), Newcastle disease vaccine (NDC), Fayoumi chicksDOI: http://dx.doi.org/10.3329/bjm.v23i2.876 Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 125-129

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