THE SUBSTANTIATION OF PARAMETERS OF IMMUNOMICROCHIP TECHNOLOGY FOR SCREENING CONTROL OF THE RESIDUAL AMOUNTS OF ANTIMICROBIAL SUBSTANCES IN AQUACULTURE

2018 ◽  
Vol 1 (2) ◽  
pp. 13-17
Author(s):  
V. S. Babunova ◽  
◽  
G. M. Goryainova ◽  
L. V. Arsen'eva ◽  
E. A. Denisova ◽  
...  
Keyword(s):  
Detection Limit ◽  
Sensitivity And Specificity ◽  
Current Practice ◽  
Optimal Parameters ◽  
Fish Products ◽  
Antimicrobial Substances ◽  
Test Systems ◽  
Antibiotic Drugs ◽  
Highly Sensitive ◽  
Sensitive Method

The article highlights the use of a highly sensitive method based on immunomicrochip technology in the current practice of veterinary and sanitary expertise of aquaculture (fish and fish products) for the presence of residual amounts of antibiotics. To do this, we used a test of Antimicrobial Array I Ultra and Antimicrobial Array II Plus from Randox (UK) in accordance with the instructions. Duration of analysis 2 ... 3 hours. Based on our studies, the optimal parameters for detecting residual quantities of various groups of antibiotics in fish using the immunomicrochip method were determined, and the sensitivity and specificity of these test systems were established. Using the Antimicrobial Array I Ultra panel, the residual amounts of 20 antibiotic drugs and sulfonamides simultaneously were determined in one sample. The detection limit in this fish panel is 1.6 to 6.5 μg / l. With the help of the Antimicrobial Array II Plus panel, the residual amounts of 35 antibacterial drugs were simultaneously determined, including 10 types of tetracycline and 17 quinolone compounds. The detection limit in fish is from 0.9 to 14.0 μg / l. In this case, the results are obtained in a quantitative equivalent, and not as a "yes-no" answer.

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

An ultra-highly sensitive and selective self-enhanced AIECL sensor for public security early warning in a nuclear emergency via a co-reactive group poisoning mechanism

2021 ◽  
Author(s):  
Ziyu Wang ◽  
Meiyun Xu ◽  
Nan Zhang ◽  
Jian-Bin Pan ◽  
Xinqi Wu ◽  
...  
Keyword(s):  
Detection Limit ◽  
Early Warning ◽  
High Selectivity ◽  
Public Security ◽  
Reactive Group ◽  
Low Detection Limit ◽  
Highly Sensitive ◽  
Vapor Sensors ◽  
Nuclear Emergency

A mechanism of co-reactive group poisoning (CGP) was discovered for designing efficient I2 vapor sensors for early warning of a nuclear emergency, which give an ultra-low detection limit of 0.13 ppt as well as high selectivity.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

scholarly journals A new highly sensitive and specific overnight combined screening and diagnostic test for primary aldosteronism

2016 ◽  
Vol 175 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Vaios Tsiavos ◽  
Athina Markou ◽  
Labrini Papanastasiou ◽  
Theodora Kounadi ◽  
Ioannis I Androulakis ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Primary Aldosteronism ◽  
Low Cost ◽  
Confirmatory Test ◽  
Outpatient Basis ◽  
Endocrine Hypertension ◽  
Serum Aldosterone ◽  
Highly Sensitive ◽  
Aldosterone To Renin Ratio

Context Primary aldosteronism (PA) is the most common cause of endocrine hypertension that is diagnosed following a two-step process: an initial screening test, based on the serum aldosterone-to-renin ratio (ARR), followed by a relatively laborious and time-consuming confirmatory test to document autonomous aldosterone (ALD) secretion. Objective The aim of this study is to develop a simple overnight test for the early and definite diagnosis of PA. Patients and methods Totally, 148 hypertensive patients underwent a fludrocortisone–dexamethasone suppression test (FDST) and the new overnight diagnostic test (DCVT) using pharmaceutical RAAS (renin–angiotensin–aldosterone system) blockade with dexamethasone, captopril and valsartan. Results Of the 148 patients, 45 were diagnosed as having PA and they all normalized their elevated blood pressure (BP) after administration of spironolactone or eplerenone. The remaining 103 patients were considered as having essential hypertension and served as controls. Using ROC analysis, the estimated sensitivity and specificity were 91 and 100%, respectively, for the post-FDST ARR, whereas 98% and 89% and 100% and 82% for the post-DCVT ARR and post-DCVT ALD, respectively, with selected cutoffs of 0.32ng/dL/μU/mL and 3ng/dL respectively. However, considering these cutoffs simultaneously, the estimated sensitivity and specificity were 98 and 100% respectively. Applying these cutoffs, the diagnosis of PA was confirmed in 44 (98%) of the 45 patients who were considered to have the disease. Conclusions In this study, a highly sensitive and specific, low-cost, rapid, safe, and easy-to-perform diagnostic test (DCVT) for PA is described, which could be utilized on an outpatient basis potentially substituting conventional laborious testing.

Highly sensitive method for concentration and absorption measurements by guided-wave resonance

2000 ◽  
Author(s):  
Guang Chen ◽  
Yi Jiang ◽  
Zhuangqi Cao ◽  
Qishun Shen ◽  
Xiaoming Dou ◽  
...  
Keyword(s):  
Guided Wave ◽  
Wave Resonance ◽  
Highly Sensitive ◽  
Sensitive Method ◽  
Absorption Measurements

Highly sensitive method for aldehydes detection: Application to furfurals analysis in raisin and bovine milk powder

2017 ◽  
Vol 987 ◽  
pp. 47-55 ◽  
Author(s):  
Yao Sun ◽  
Zhaobing Guan ◽  
Hongwei Cai ◽  
Yiyong Huang ◽  
Yawei Lin ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Milk Powder ◽  
Highly Sensitive ◽  
Sensitive Method

scholarly journals 19F NMR Spectroscopy as a Highly Sensitive Method for the Direct Monitoring of Confined Crystallization within Nanoporous Materials

2016 ◽  
Vol 55 (31) ◽  
pp. 8904-8908 ◽  
Author(s):  
Karol P. Nartowski ◽  
Diksha Malhotra ◽  
Lucy E. Hawarden ◽  
Juraj Sibik ◽  
Dinu Iuga ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Nanoporous Materials ◽  
19F Nmr ◽  
19F Nmr Spectroscopy ◽  
Confined Crystallization ◽  
Highly Sensitive ◽  
Sensitive Method

scholarly journals Newly Developed Highly Sensitive Method for the Determination of Capecitabine by Using UV-Spectroscopy

2019 ◽  
Vol 11 (03) ◽  
Author(s):  
Manas Ranjan Mishra ◽  
Punam Agrawal ◽  
Surya Narayan Das
Keyword(s):  
Colon Cancer ◽  
Uv Spectroscopy ◽  
First Line ◽  
Spectrophotometric Methods ◽  
Advanced Colon Cancer ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Solvent Systems ◽  
Sensitive Method

Capecitabine is a 'pro-drug' to the cytotoxic agent 5-fluorouracil (5-FU) intended to administered orally. Capecitabine is generally used as first line monotherapy for advanced colon cancer. Simple, rapid, accurate UV spectrophotometric methods were developed in the present study and validated for the estimation of Capecitabine in bulk and its formulations as per ICH guidelines. Three solvent systems viz., 0.1N NaOH, 0.1N HCl and Methanol: Water (1:3) was tried. The results suggest that the developed method shows linearity over the range of concentration 2-24μg/ml and a correlation coefficient of 0.9999. Accuracy, precision, linearity, robustness, and ruggedness were statistically validated as per ICH guidelines for all the developed methods. The % RSD values for validated methods were found to be less than 1.5 and methods will find application in routine analysis of drug formulations containing Capecitabine.

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