DEVELOPING OF THE MODES AND TECHNOLOGY FOR DESINFECTION OF VETERINARY AND SANITARY OJECTS BY DIRECTED AEROSOLS ANOLYTE «ALOX» IN CHAMBER EXPERIENCES

2019 ◽  
Vol 1 (2) ◽  
pp. 151-157
Author(s):  
N. E. Vanner ◽  
◽  
A. A. Prokopenko ◽  
G. V. Filipenkova ◽  
I. V. Kushch ◽  
...  
Keyword(s):  
Flow Rate ◽  
Laboratory Experiments ◽  
Drug Consumption ◽  
E Coli ◽  
Test Objects

As a result of the experiments on test objects made of wood, concrete and iron with protein protection, contaminated with microorganisms of the 1...4th groups of resistance to chemical disinfectants is studing the disinfecting activity of the new anolyte "Alox". It is established that the drug "Alox" has a high bactericidal and disinfecting activity. In laboratory experiments, test objects made of wood, concrete and iron, contaminated with E. coli, Pcs. 1257, were disinfected with directed aerosols of the drug "Alox" for 6 hours at a rate of 100 ml/m2. Disinfection of test objects with protein protection, contaminated with S. aureus, pcs. 209-P, was reached by anolyte in 6 hours with a drug consumption of 150 ml / m2, Mycobacterium B-5 – with a preparation consumption of 170 ml/m2 in 24 hours, and 200 ml/m2 in 6 hours. Inactivation of spores B. cereus, pcs. 96 on the test objects is achieved by directed aerosols of the drug "Alox" for 24 hours at a flow rate of 200 ml/m2.

STUDY OF BACTERICIDAL AND DISINFECTING ACTIVITY OF PEROX ANOLITE IN CHAMBER EXPERIMENTS

2021 ◽  
Vol 1 (3) ◽  
pp. 295-302
Author(s):  
A.A. Prokopenko ◽  
◽  
N.E. Wanner ◽  
I.V. Kushch ◽  
G.V. Filipenkova ◽  
...  
Keyword(s):  
Flow Rate ◽  
Bactericidal Activity ◽  
Drug Consumption ◽  
Active Chlorine ◽  
E Coli ◽  
Test Objects

Previously, it was found that anolyte Perox has a high bactericidal and disinfecting activity. Thus, the bactericidal activity of anolyte containing 500 mg/l of active chlorine with mineralization of 0.1-0.2 % and pH of 6.0 in relation to E. coli bacteria, pcs. 1257 – 0.0976%, and in relation to S. aureus, pcs. 209 – P – 0.1953%. Inactivation of E. coli bacteria on contaminated test objects made of wood, concrete, and iron was provided by directional aerosols of anolyte Perox in 6 hours at a flow rate of 100 ml/m2. Disinfection of test objects with protein protection contaminated with S. aureus pcs. 209-P, occurred in 6 hours at anolyte consumption of 150 ml/m2, and Mycobacterium B-5 – in 6 and 24 hours, with a drug consumption of 200 and 170 ml/ m2, respectively. Destruction spores of B. cereus pcs. 96 occurred with an exposure of 24 hours with drug consumption of 200 ml/m2. As a result of the conducted chamber experiments, we have determined effective modes of disinfection of test objects with protein protection, contaminated with microorganisms of the 1st – 4th groups of resistance.

STUDY OF BACTERICIDAL AND DISINFECTING ACTIVITY OF DIRECTED AEROSOLS OF THE PREPARATION UK-ANOLIT

2021 ◽  
Vol 1 (4) ◽  
pp. 420-427
Author(s):  
Alexander A. Prokopenko ◽  
◽  
Irina V. Kushch ◽  
Natalya E. Vanner ◽  
Galina V. Filipenkova ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Laboratory Experiments ◽  
Drug Consumption ◽  
E Coli ◽  
New Drug ◽  
Test Objects

The article presents the results of studying the bactericidal and disinfecting activity of directed aerosols of the new drug UK-Anolyte in laboratory experiments. It was found that preparation UK-Anolyte has a high bactericidal and disinfectant activity. Bactericidal activity of UK-Anolyte against E. coli bacteria, pcs. 1257, is 0.0976%, and in relation to S. aureus, pcs. 209-P, – 0.1953%. Disinfection of test objects with protein protection was achieved with anolyte at a drug consumption of 130...250 ml/m2 for 3...6 hours.

STUDYING THE BACTERICIDAL AND DISINFECTING ACTIVITY OF THE NEW ENVIRONMENTALLY SAFE PREPARATION ANOLYTE ANK SUPER

2019 ◽  
Vol 1 (3) ◽  
pp. 287-293
Author(s):  
A.A. Prokopenko ◽  
◽  
N.E. Vanner ◽  
G.V. Filipenkova ◽  
◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Laboratory Experiments ◽  
E Coli ◽  
Veterinary Practice ◽  
Russian Academy Of Sciences ◽  
Environmentally Safe ◽  
Academy Of Sciences ◽  
Test Objects

The aim of the work is to study the bactericidal and disinfecting activity of the new environmentally safe preparation Anolyte ANK Super in laboratory experiments. It is established that the preparation has a high bactericidal and disinfectant activity. Thus, the bactericidal activity of the anolyte against bacteria E. coli (pc.1257) and S. aureus (pc. 209-P) is 0,0977 and 0,1953% respectively. Directed aerosols of the preparation Anolyte ANK Super at a rate of 0,15...0,3 l/ m2 disinfect the surface of the test objects contaminated with vegetative and spore microflora for 3...24 hours. For veterinary practice, “The technology of disinfection of objects of veterinary supervision by directed aerosols of the preparation Anolyte ANK Super (Approved by Russian Academy of Sciences in 23.11.2017) was developed and approved.

Elimination of viruses, bacteria and protozoan oocysts by slow sand filtration

2004 ◽  
Vol 50 (1) ◽  
pp. 147-154 ◽  
Author(s):  
W.A.M. Hijnen ◽  
J.F. Schijven ◽  
P. Bonné ◽  
A. Visser ◽  
G.J. Medema
Keyword(s):  
Pilot Plant ◽  
Pathogenic Bacteria ◽  
Laboratory Experiments ◽  
Full Scale ◽  
Sand Filters ◽  
E Coli ◽  
Thermotolerant Coliforms ◽  
Filter Bed ◽  
Scale Data ◽  
Bacteria Removal

The decimal elimination capacity (DEC) of slow sand filters (SSF) for viruses, bacteria and oocysts of Cryptosporidium has been assessed from full-scale data and pilot plant and laboratory experiments. DEC for viruses calculated from experimental data with MS2-bacteriophages in the pilot plant filters was 1.5-2 log10. E. coli and thermotolerant coliforms (Coli44) were removed at full-scale and in the pilot plant with 2-3 log10. At full-scale, Campylobacter bacteria removal was 1 log10 more than removal of Coli44, which indicated that Coli44 was a conservative surrogate for these pathogenic bacteria. Laboratory experiments with sand columns showed 2-3 and >5-6 log10 removal of spiked spores of sulphite-reducing clostridia (SSRC; C. perfringens) and oocysts of Cryptosporidium respectively. Consequently, SSRC was not a good surrogate to quantify oocyst removal by SSF. Removal of indigenous SSRC by full-scale filters was less efficient than observed in the laboratory columns, probably due to continuous loading of these filter beds with spores, accumulation and retarded transport. It remains to be investigated if this also applies to oocyst removal by SSF. The results additionally showed that the schmutzdecke and accumulation of (in)organic charged compounds in the sand increased the elimination of microorganisms. Removal of the schmutzdecke reduced DEC for bacteria by ±2 log10, but did not affect removal of phages. This clearly indicated that, besides biological activity, both straining and adsorption were important removal mechanisms in the filter bed for microorganisms larger than viruses.

scholarly journals Seasonal antimicrobial activity of volatile substances emitted by the representatives of Begonia L. genus (Begoniaceae)

2021 ◽  
Vol 10 (1) ◽  
pp. 167-172
Author(s):  
Natalya Vladimirovna Tsybulya ◽  
Tatyana Dmitrievna Fershalova
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Antimicrobial Action ◽  
Indoor Environments ◽  
Test Species ◽  
E Coli ◽  
Moderate Growth ◽  
Autumn And Winter ◽  
Test Objects

The paper continues multi-year integrated studies of the numerous taxons of tropical Begonia genus, which allowed us to evaluate the adaptive capacity of the representatives of this genus relying on the revealed morphological, rhythmological and biochemical features. The investigation of antimicrobial activity of intact plants and different fractions of plant extracts allowed us to distinguish several promising representatives of this genus exhibiting activity against a broad range of test microorganisms. The involvement of these plants within phytomodules in child care centers caused a substantial decrease in total microbial contamination of the air. The paper contains results of the experimental investigation of seasonal antimicrobial activity of 13 species, 8 hybrids. The phytoncide activity was measured by exposing the streak cultures of microbial test species bacteria Staphylococcus epidermidis, Esсherichia coli and yeast-like fungi Candida albicans to the volatile emissions of plants. It has been established that 95% of begonias are distinguished by the pronounced activity against S. epidermidis bacteria, 48% against E. coli bacteria and 43% against fungi Candida albicans. The seasonal specificity of the antimicrobial action of begonias was detected: the growth of the colonies of S. epidermidis bacteria is inhibited to the highest extent during spring and summer, at the phase of intense growth, while the activity against E. coli is the highest during autumn and winter at the phase of moderate growth. The taxons with clearly pronounced, long-term and universal antimicrobial action to the studied test objects were revealed and recommended for practical planting in various types of indoor environments. The data obtained in the study may serve as the basis for further investigation of the chemical composition of volatile exometabolites by the example of the representatives of Gireoudia and Coelocentrum sections that have exhibited high antimicrobial activity against S. epidermidis and E. coli.

scholarly journals Sex overrides mutation in Escherichia coli colonizing the gut

2018 ◽  
Author(s):  
N. Frazão ◽  
A. Sousa ◽  
M. Lässig ◽  
I. Gordo
Keyword(s):  
Escherichia Coli ◽  
Experimental Evolution ◽  
Temporal Pattern ◽  
Driving Force ◽  
Laboratory Experiments ◽  
Rapid Evolution ◽  
Point Mutations ◽  
Natural Environments ◽  
E Coli ◽  
Accelerate Evolution

AbstractBacteria evolve by mutation accumulation in laboratory experiments, but the tempo and mode of evolution in natural environments are largely unknown. Here we show, by experimental evolution of E. coli in the mouse gut, that the ecology of the gut controls bacterial evolution. If a resident E. coli strain is present in the gut, an invading strain evolves by rapid horizontal gene transfer; this mode precedes and outweighs evolution by point mutations. An epidemic infection by two phages drives gene uptake and produces multiple co-existing lineages of phage-carrying (lysogenic) bacteria. A minimal dynamical model explains the temporal pattern of phage epidemics and their complex evolutionary outcome as generic effects of phage-mediated selection. We conclude that phages are an important eco-evolutionary driving force – they accelerate evolution and promote genetic diversity of bacteria.One Sentence SummaryBacteriophages drive rapid evolution in the gut.

scholarly journals Efficient Escherichia coli (E. coli) Trapping and Retrieval from Bodily Fluids via a Three-Dimensional (3D) Microbeads Stacked Nano-Device

2019 ◽  
Author(s):  
Xinye Chen ◽  
Abbi miller ◽  
Shengting Cao ◽  
Yu Gan ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Rate ◽  
Magnetic Beads ◽  
Three Dimensional ◽  
E Coli ◽  
Bodily Fluids ◽  
3D Space ◽  
Fluidic Device ◽  
Computational Fluid Dynamics Cfd ◽  
On Chip

<div>A micro- and nano-fluidic device stacked with magnetic beads is developed to efficiently trap, concentrate, and retrieve Escherichia coli (E. coli) from bacteria suspension</div><div>and pig plasma. The small voids between the magnetic beads are used to physically isolate the bacteria in the device. We use computational fluid dynamics (CFD), 3D</div><div>tomography technology, and machine learning to probe and explain the bead stacking in a small 3D space with various flow rates. A combination of beads with different sizes is utilized to achieve a high capture efficiency of ~86% with a flow rate of 50 μL/min. Leveraging the high deformability of this device, the E. coli sample is retrieved from the designated bacteria suspension by applying a higher flow rate, followed by rapid magnetic separation. This unique function is also utilized to concentrate E. coli from the original bacteria suspension. An on-chip concentration</div><div>factor of ~11× is achieved by inputting 1,300 μL of the E. coli sample and then concentrating it in 100 μL buffer.</div><div>Importantly, this multiplexed, miniaturized, inexpensive, and transparent device is easy to fabricate and operate, making it ideal for pathogen separation in both laboratory and pointof- care (POC) settings.</div>

scholarly journals Efficient Escherichia coli (E. coli) Trapping and Retrieval from Bodily Fluids via a Three-Dimensional (3D) Microbeads Stacked Nano-Device

2019 ◽  
Author(s):  
Xinye Chen ◽  
Abbi miller ◽  
Shengting Cao ◽  
Yu Gan ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Rate ◽  
Magnetic Beads ◽  
Three Dimensional ◽  
E Coli ◽  
Bodily Fluids ◽  
3D Space ◽  
Fluidic Device ◽  
Computational Fluid Dynamics Cfd ◽  
On Chip

<div>A micro- and nano-fluidic device stacked with magnetic beads is developed to efficiently trap, concentrate, and retrieve Escherichia coli (E. coli) from bacteria suspension</div><div>and pig plasma. The small voids between the magnetic beads are used to physically isolate the bacteria in the device. We use computational fluid dynamics (CFD), 3D</div><div>tomography technology, and machine learning to probe and explain the bead stacking in a small 3D space with various flow rates. A combination of beads with different sizes is utilized to achieve a high capture efficiency of ~86% with a flow rate of 50 μL/min. Leveraging the high deformability of this device, the E. coli sample is retrieved from the designated bacteria suspension by applying a higher flow rate, followed by rapid magnetic separation. This unique function is also utilized to concentrate E. coli from the original bacteria suspension. An on-chip concentration</div><div>factor of ~11× is achieved by inputting 1,300 μL of the E. coli sample and then concentrating it in 100 μL buffer.</div><div>Importantly, this multiplexed, miniaturized, inexpensive, and transparent device is easy to fabricate and operate, making it ideal for pathogen separation in both laboratory and pointof- care (POC) settings.</div>

LABORATORY TESTS OF DISINFECTANT «MONOCHLORIDE (IODINE CHLORIDE) 2%»

2018 ◽  
Vol 1 (4) ◽  
pp. 27-33
Author(s):  
N. I. Popov ◽  
◽  
A. V. Suvorov ◽  
S. M. Lobanov ◽  
S. A. Michko ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Laboratory Tests ◽  
Consumption Rate ◽  
Laboratory Work ◽  
Laboratory Studies ◽  
E Coli ◽  
Iodine Chloride ◽  
Test Objects ◽  
Test Cultures

The article describes the results of laboratory tests of the effectiveness of the disinfectant Monochloride (Iodine chloride) 2%. Laboratory studies were carried out on test objects and test surfaces contaminated with test cultures of microorganisms, which included museum cultures of E. coli (E. coli 1257), S. aureus 209-P, mycobacteria (pcs. B5), and spores (B. cereus pieces. 96). Disinfection of test objects was carried out by the method of irrigation at a consumption rate of 0,25...0,3 l/ m2 with disinfection of smooth surfaces and 0,5 l/m2, with disinfection of rough surfaces. The treatment was performed twice with an interval of 60 minutes. Our work established that the Monochloride (Iodine chloride) 2% has a high disinfectant activity against Escherichia coli, Staphylococcus aureus, mycobacteria and spores. On the basis of the laboratory work, this tool can be recommended for production tests at veterinary surveillance facilities.

Sign in / Sign up

Export Citation Format

Share Document