scholarly journals Occurence of target site resistance to glyphosate in goosegrass (Eleusine indica (L.) Gaertn.) in Saitama prefecture, Japan

2021 ◽  
Vol 66 (1) ◽  
pp. 11-15
Author(s):  
Kazuyuki Tanno
Keyword(s):  
Target Site ◽  
Eleusine Indica ◽  
Target Site Resistance

scholarly journals Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation

2012 ◽  
Vol 30 (3) ◽  
pp. 675-681 ◽  
Author(s):  
M.D. Osuna ◽  
I.C.G.R. Goulart ◽  
R.A. Vidal ◽  
A. Kalsing ◽  
J.P. Ruiz Santaella ◽  
...  
Keyword(s):  
Amino Acid Position ◽  
Target Site ◽  
In Vitro Assays ◽  
Site Mutation ◽  
Eleusine Indica ◽  
Herbicide Resistant ◽  
Frequent Use ◽  
Target Site Resistance ◽  
Grass Weed

Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078 (referred to EMBL accession no. AJ310767) and resulted in the triplet of glycine. These results constitute the first report on a target site mutation for a Brazilian herbicide resistant grass weed.

Evolution of multiple target‐site resistance mechanisms in individual plants of glyphosate‐resistant Eleusine indica from China

2021 ◽  
Author(s):  
Chun Zhang ◽  
Chao‐jie Yu ◽  
Qin Yu ◽  
Wen‐lei Guo ◽  
Tai‐jie Zhang ◽  
...  
Keyword(s):  
Resistance Mechanisms ◽  
Target Site ◽  
Multiple Target ◽  
Eleusine Indica ◽  
Target Site Resistance

Characterisation of target-site resistance to ACCase-inhibiting herbicides in the weedAlopecurus myosuroides (black-grass)

2003 ◽  
Vol 59 (2) ◽  
pp. 190-201 ◽  
Author(s):  
Stephen R Moss ◽  
Kay M Cocker ◽  
Amanda C Brown ◽  
Linda Hall ◽  
Linda M Field
Keyword(s):  
Target Site ◽  
Target Site Resistance

Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields

2021 ◽  
Author(s):  
Norazua Zakaria ◽  
Rabiatuladawiyah Ruzmi ◽  
Salmah Moosa ◽  
Norhayu Asib ◽  
Dzarifah Zulperi ◽  
...  
Keyword(s):  
Rice Fields ◽  
Target Site ◽  
Invasive Weed ◽  
Target Site Resistance

Controlling Herbicide-Resistant Annual Bluegrass (Poa annua) Phenotypes with Methiozolin

2017 ◽  
Vol 31 (3) ◽  
pp. 470-476 ◽  
Author(s):  
James T. Brosnan ◽  
Jose J. Vargas ◽  
Gregory K. Breeden ◽  
Sarah L. Boggess ◽  
Margaret A. Staton ◽  
...  
Keyword(s):  
Acetolactate Synthase ◽  
Target Site ◽  
Multiple Resistance ◽  
Herbicide Resistant ◽  
Annual Bluegrass ◽  
Microtubule Inhibitors ◽  
Effective Option ◽  
Target Site Resistance ◽  
Susceptible Control ◽  
Als Inhibitors

Methiozolin is an isoxazoline herbicide being investigated for selective POST annual bluegrass control in managed turfgrass. Research was conducted to evaluate methiozolin efficacy for controlling two annual bluegrass phenotypes with target-site resistance to photosystem II (PSII) or enolpyruvylshikimate-3-phosphate synthase (EPSPS)-inhibiting herbicides (i.e., glyphosate), as well as phenotypes with multiple resistance to microtubule and EPSPS or PSII and acetolactate synthase (ALS)-inhibiting herbicides. All resistant phenotypes were established in glasshouse culture along with a known herbicide-susceptible control and treated with methiozolin at 0, 125, 250, 500, 1000, 2000, 4000, or 8000 g ai ha−1. Methiozolin effectively controlled annual bluegrass with target-site resistance to inhibitors of EPSPS, PSII, as well as multiple resistance to EPSPS and microtubule inhibitors. Methiozolin rates required to reduce aboveground biomass of these resistant phenotypes 50% (GR50 values) were not significantly different from the susceptible control, ranging from 159 to 421 g ha−1. A phenotype with target-site resistance to PSII and ALS inhibitors was less sensitive to methiozolin (GR50=862 g ha−1) than a susceptible phenotype (GR50=423 g ha−1). Our findings indicate that methiozolin is an effective option for controlling select annual bluegrass phenotypes with target-site resistance to several herbicides.

scholarly journals A high diversity of mechanisms endows ALS-inhibiting herbicide resistance in the invasive common ragweed (Ambrosia artemisiifolia L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ingvild Loubet ◽  
Laëtitia Caddoux ◽  
Séverine Fontaine ◽  
Séverine Michel ◽  
Fanny Pernin ◽  
...  
Keyword(s):  
Resistance Mechanism ◽  
Resistance Management ◽  
Genotyping By Sequencing ◽  
Ambrosia Artemisiifolia ◽  
Target Site ◽  
Major Type ◽  
Common Ragweed ◽  
Resistance Evolution ◽  
Arable Weed ◽  
Target Site Resistance

AbstractAmbrosia artemisiifolia L. (common ragweed) is a globally invasive, allergenic, troublesome arable weed. ALS-inhibiting herbicides are broadly used in Europe to control ragweed in agricultural fields. Recently, ineffective treatments were reported in France. Target site resistance (TSR), the only resistance mechanism described so far for ragweed, was sought using high-throughput genotyping-by-sequencing in 213 field populations randomly sampled based on ragweed presence. Additionally, non-target site resistance (NTSR) was sought and its prevalence compared with that of TSR in 43 additional field populations where ALS inhibitor failure was reported, using herbicide sensitivity bioassay coupled with ALS gene Sanger sequencing. Resistance was identified in 46 populations and multiple, independent resistance evolution demonstrated across France. We revealed an unsuspected diversity of ALS alleles underlying resistance (9 amino-acid substitutions involved in TSR detected across 24 populations). Remarkably, NTSR was ragweed major type of resistance to ALS inhibitors. NTSR was present in 70.5% of the resistant plants and 74.1% of the fields harbouring resistance. A variety of NTSR mechanisms endowing different resistance patterns evolved across populations. Our study provides novel data on ragweed resistance to herbicides, and emphasises that local resistance management is as important as mitigating gene flow from populations where resistance has arisen.

scholarly journals Impact of a Novel W2027L Mutation and Non-Target Site Resistance on Acetyl-CoA Carboxylase-Inhibiting Herbicides in a French Lolium multiflorum Population

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1838
Author(s):  
Shiv Shankhar Kaundun ◽  
Joe Downes ◽  
Lucy Victoria Jackson ◽  
Sarah-Jane Hutchings ◽  
Eddie Mcindoe
Keyword(s):  
Lolium Multiflorum ◽  
Restriction Enzymes ◽  
Target Site ◽  
Cereal Crops ◽  
Acetyl Coa Carboxylase ◽  
Wild Type ◽  
Resistance Mutations ◽  
Acetyl Coa ◽  
Pcr Product ◽  
Target Site Resistance

Herbicides that inhibit acetyl-CoA carboxylase (ACCase) are among the few remaining options for the post-emergence control of Lolium species in small grain cereal crops. Here, we determined the mechanism of resistance to ACCase herbicides in a Lolium multiflorum population (HGR) from France. A combined biological and molecular approach detected a novel W2027L ACCase mutation that affects aryloxyphenoxypropionate (FOP) but not cyclohexanedione (DIM) or phenylpyraxoline (DEN) subclasses of ACCase herbicides. Both the wild-type tryptophan and mutant leucine 2027-ACCase alleles could be positively detected in a single DNA-based-derived polymorphic amplified cleaved sequence (dPACS) assay that contained the targeted PCR product and a cocktail of two discriminating restriction enzymes. Additionally, we identified three well-characterised I1781L, I2041T, and D2078G ACCase target site resistance mutations as well as non-target site resistance in HGR. The non-target site component endowed high levels of resistance to FOP herbicides whilst partially impacting on the efficacy of pinoxaden and cycloxydim. This study adequately assessed the contribution of the W2027L mutation and non-target site mechanism in conferring resistance to ACCase herbicides in HGR. It also highlights the versatility and robustness of the dPACS method to simultaneously identify different resistance-causing alleles at a single ACCase codon.

Functional Genomics Analysis of Horseweed (Conyza canadensis) with Special Reference to the Evolution of Non–Target-Site Glyphosate Resistance

Weed Science ◽  
2010 ◽  
Vol 58 (2) ◽  
pp. 109-117 ◽  
Author(s):  
Joshua S. Yuan ◽  
Laura L. G. Abercrombie ◽  
Yongwei Cao ◽  
Matthew D. Halfhill ◽  
Xin Zhou ◽  
...  
Keyword(s):  
Functional Genomics ◽  
Molecular Mechanisms ◽  
Glyphosate Resistance ◽  
Resistance Mechanisms ◽  
Target Site ◽  
Environmentally Benign ◽  
Conyza Canadensis ◽  
Target Site Resistance ◽  
Weedy Species ◽  
Heterologous Microarray

The evolution of glyphosate resistance in weedy species places an environmentally benign herbicide in peril. The first report of a dicot plant with evolved glyphosate resistance was horseweed, which occurred in 2001. Since then, several species have evolved glyphosate resistance and genomic information about nontarget resistance mechanisms in any of them ranges from none to little. Here, we report a study combining iGentifier transcriptome analysis, cDNA sequencing, and a heterologous microarray analysis to explore potential molecular and transcriptomic mechanisms of nontarget glyphosate resistance of horseweed. The results indicate that similar molecular mechanisms might exist for nontarget herbicide resistance across multiple resistant plants from different locations, even though resistance among these resistant plants likely evolved independently and available evidence suggests resistance has evolved at least four separate times. In addition, both the microarray and sequence analyses identified non–target-site resistance candidate genes for follow-on functional genomics analysis.

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