scholarly journals Assessment of Semen Characteristics Among Three Phenotypes of Chicken Raised in Akko, Gombe State of Nigeria

2021 ◽  
Vol 12 (1) ◽  
pp. 40-48
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Sadiq Haladu ◽  
Asmad Kari
Keyword(s):  
Artificial Insemination ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Sperm Abnormalities ◽  
Normal Sperm ◽  
Poultry Breeding ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Abdominal Massage

In poultry breeding program the success of artificial insemination (AI) is highly influenced by  the quality of semen. The aim of the present research was to evaluate the variations in semen characteristics of three phenotypes of chicken of the same specie raised in Akko, Nigeria. A total of 9 cocks from threedifferent phenotypes; Red feathered (n = 3), White feathered (n = 3) and Black feathered (n =3). The semen was evaluated for macroscopic (i.e. semen volume and colour) and microscopic (i.e. sperm concentration, motility and morphology) criteria after being collected by abdominal massage method. There were phenotypes variation (P > 0.05)effects on semen motility, number of live/dead sperm and sperm abnormalities. No differences(P>0.05) were observed on volume, colour, concentration, mass motility and pH of semen. The observed sperm progressive motility was ranged from 90.5 ±1.21% to 95.09±0.82%. The White feathered strain had the highest sperm progressive motility (P<0.05)and highest value for live and normal sperm (93.5±0.63% and 87.90±0.25%), while the black feathered had the least and (92.4±0.73% and 85.5±0.50%) respectively. The Red feathered strain had the highest value for both dead and sperm head defect (9.2±0.33%and 7.0±0.52%). Whilst, the Black feathered strain scored the highest percentage of both sperm tail and neck defects with values ranged from 11.4±0.43%to 14.2±1.08% and 19.4±0.53% to 23.6±0.69%, respectively. This study suggests that there are large variations present in semen characteristics of different phenotypes of cocks; White feathered strain is likely have better semen characteristics compared to Red and Black feathered strains. Therefore, White feathered strain can potentially be used in artificial insemination (AI) for chicken production and improvement.

scholarly journals Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria

2019 ◽  
Vol 15 (2-1) ◽  
pp. 303-306
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Ma’aruf Bashir Sani
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Indigenous Breed ◽  
Normal Sperm ◽  
Abnormal Sperm ◽  
Significant Difference ◽  
Semen Characteristics ◽  
Genetically Improved ◽  
Abdominal Massage

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ±  to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.

116. EFFECTS OF SUPPLEMENTAL VITAMIN ON THE SEMEN CHARACTERISTICS OF MARKHOZ GOATS DURING NON-BREEDING SEASON

2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi
Keyword(s):  
Vitamin E ◽  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Supplemental Vitamin ◽  
Diet Control ◽  
Live Sperm

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.

Effect of genetic and environmental factors on semen productionand quality of Madura cattle in Indonesia

2016 ◽  
Author(s):  
Koko Wisnu Prihatin ◽  
Luqman Hakim ◽  
Sucik Maylinda ◽  
V.M. Ani Nurgiartiningsih
Keyword(s):  
Environmental Factors ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Climatic Conditions ◽  
Bull Semen ◽  
Semen Collection ◽  
Semen Volume ◽  
Ejaculation Frequency ◽  
Genetic And Environmental Factors

The indigenous Madura cattle semen production and quality were evaluated. Total of 2275 semen collection records from 11 bulls were analyzed to evaluate the genetic potency of semen productions. The genetic evaluation was described as an estimation of bull semen volume, sperm concentration and sperm individual motility. Data were analyzed by HGLM-REML with environmental factors of age, seasons, interval of semen collections and frequency of ejaculations as fixed effect. The result showed that the age and the ejaculation frequency affected to all variables; an interval of semen collections affected to both semen volume and sperm concentrations; and seasons only affected to sperm concentrations. Repeatability estimate of semen volume, sperm concentration and sperm individual motility were 0.376, 0.445 and 0.567 respectively. It can be concluded that the quality of ejaculate was less affected by environmental factors, supported by the good adaptation of indigenous Madura cattle under the local climatic conditions and management in the artificial insemination station.

scholarly journals COMPARATIVE STUDIES OF THE REPRODUCTIVE CAPACITY OF NIGERIAN AND EXOTIC POULTRY BREEDS

2021 ◽  
Vol 6 ◽  
pp. 47-51
Author(s):  
G. N. Egbunike ◽  
J. A. Oluyemi
Keyword(s):  
Rhode Island ◽  
Sperm Concentration ◽  
Time Effect ◽  
Reproductive Capacity ◽  
Testis Weight ◽  
White Leghorn ◽  
Progressive Motility ◽  
Indigenous Breed ◽  
Sperm Abnormalities ◽  
Semen Characteristics

THE study involved 48 adult cocks from four breeds namely, White Rock (WR), Rhode Island Red (RIR), White Leghorn (WL) and Nigerian indigenous breed (NIB). They weighed between 1 -26kg at the beginning of the experiment. The WR cock had the heaviest paired testis Weight (25.06g) while NIB had the lightest (16.549). These weights accounted for 1.04% and 1.22% of the liveweight respectively while the specific gravity of the testis from these two breeds was 1.048. The volume (0.63ml vs 0.33ml) and progressive motility of the spermatozoa (63.34 vs 58.12%) were highest in the WR and lowest in the NIB while sperm concentration (3.03 vt 2.43 x 10') was highest also in the WR but lowest in the WL. These semen characteristics were significantly influenced by breed and time of ejaculation except with the volume in which the time effect was Insignificant. The WR semen had the lowest sperm abnormalities (10.13% while RIR had the highest (13.14%). The results however Indicate that the WR cocks ejaculated the largest quantity and best quality semen, followed in order by RIR, NIB and WL 

80 Use of an Electronically-controlled Floor Cooling Pad During Heat Stress on Thermoregulatory and Reproductive Performance in Boars

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 107-107
Author(s):  
Larissa K Shirley ◽  
Tyler Field ◽  
Allan P Schinckel ◽  
Jay S Johnson ◽  
Robert Stwalley ◽  
...  
Keyword(s):  
Heat Stress ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Controlled Cooling ◽  
Ir Camera ◽  
Progressive Motility ◽  
Normal Sperm ◽  
Semen Volume ◽  
Negative Impacts ◽  
Kinematic Motion

Abstract This study was designed to evaluate the effects of electronically-controlled floor cooling pads on thermoregulatory and reproductive parameters in boars during heat stress (HS). Boars (n = 24) were randomly assigned to crates with non-functional pads (CON) or pads that were flushed in either 8-min intervals or when the pad reached 28.5°C (FLUSH). For 3 d, boars were subjected to cyclical HS (28 to 35°C; &gt;65% relative humidity). Boars were fed 2.4 kg/d and daily feed intake was recorded. Respiration rate (RR), rectal temperature (Rtemp) and skin temperature were recorded every 2 h during HS (via IR camera), testicular temperature was recorded twice daily. Semen was collected d7 and d14 before HS, the day following HS and weekly for 6 weeks and evaluated for volume, sperm concentration, motility, progressive motility, morphological abnormalities, and viability. After 2 h of HS, FLUSH boars had reduced RR (P &lt; 0.001) and RTemp (P &lt; 0.001) when compared to control boars, and this difference was maintained throughout HS. Skin and testicular temperature were reduced in FLUSH vs. CON boars after 6 h of HS (P &lt; 0.05). Semen volume was greater in FLUSH vs CON boars (P = 0.01) resulting in a tendency for an increase in total sperm per ejaculate (P = 0.075). From weeks 2 to 5 post-HS, FLUSH boars had increased motility (P = 0.006) and progressive motility (P = 0.001), with corresponding increases in sperm kinematic motion parameters when compared to CON boars. The number of morphologically normal sperm cells were increased (P = 0.006) in FLUSH vs CON boars due to reduced distal droplets (P = 0.033) and proximal droplets (P &lt; 0.001). Abnormal acrosomes were reduced (P &lt; 0.001) in FLUSH vs CON boars at week 3 post-HS. In summary, electronically controlled cooling pads effectively reduced negative thermoregulatory indicators of HS and minimized or removed the negative impacts of HS on semen quality in boars.

scholarly journals The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition

2020 ◽  
Vol 45 (3) ◽  
pp. 197-205
Author(s):  
I. Darussalam ◽  
R. I. Arifiantini ◽  
I. Supriatna ◽  
R. S. D. Rasad
Keyword(s):  
Sperm Concentration ◽  
Egg Yolk ◽  
Carnitine Supplementation ◽  
Equal Part ◽  
Liquid Form ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Bull Semen ◽  
Sperm Abnormalities

The study was conducted to investigatethe effect of L-Carnitine supplementation in Tris-eggyolk (TEY) diluents to optimize the quality of Pasundan bull liquid semen. Semen samples were collected from three Pasundan bulls (6 – 7 years old)usinganartificial vagina. Semen samples were evaluated macroscopically and microscopically, semen having ≥70% progressive motility, ≥500×106ml-1 sperm concentration and ≤ 20% sperm abnormalities were divided into 5 equal part. Each part  wasdiluted with TEYand supplemented with0 mM(control), 1 mM, 2 mM, 3 mM and 4 mM L-Carnitine. Liquidsemen was storedat5°C.Sperm motility was evaluated every 12 h  objectively using Computer Asissted Sperm Analysis (CASA) until progressive motilityreached 40%. The best diluent was demonstratedby progressive motility (PM)by TEY supplemented with 1 mM L-Carnitine(43.08±0.49%) that remained up to 108 h compared tothecontrol group (43.63±0.70%) that remained up to 72 h (P<0.05). In conclusion, 1 mM L-Carnitine in TEY was the best concentration for preservation of Pasundan bull semen stored in the liquid form. 

scholarly journals The relationship between serum hormone levels (follicle-stimulating hormone, luteinizing hormone, total testosterone) and semen parameters

2015 ◽  
Vol 87 (3) ◽  
pp. 194 ◽  
Author(s):  
Mehmet Zeynel Keskin ◽  
Salih Budak ◽  
Tuǧba Zeyrek ◽  
Orçun Çelik ◽  
Oguz Mertoglu ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Reference Value ◽  
Semen Parameters ◽  
Total Testosterone ◽  
Testosterone Levels ◽  
Progressive Motility ◽  
Semen Volume ◽  
Significant Difference ◽  
Serum Hormone ◽  
Serum Gonadotropin

Objective: The aim of this study was to investigate the effect of serum gonadotropin and total testosterone levels on semen parameters. Materials and Methods: Three hundred and eighty-two patients that applied to a male infertility polyclinic were included in our study. Serum gonadotropin and total testosterone levels and semen parameters of the patients were analyzed during the first visit to the clinic. The reference FSH value was 1.5-12.4 mIU/mL, that of LH was 1.7-8.6 mIU/mL and the reference value for total testosterone was 249-836 ng/dL. Results: While there was no statistically significant difference between the patients with low gonadotropin levels and the controls regarding any of the semen parameters (p &gt; 0.05), there was a strong statistically significant difference between the patients with high gonadotropin levels and the controls regarding sperm concentration (p = 0.000), total motility (p = 0.000), progressive motility (p = 0.000), and morphology (p = 0.000). There was a strong statistically significant difference between the patients with low testosterone levels and the controls regarding total motility (p = 0.012) and progressive motility (p = 0.010), and a weak statistically significant difference in morphology (p = 0.042). There was no statistically significant difference in semen volume or sperm concentration (p &gt; 0.05). There was no statistically significant difference in any of the semen parameters between the patients with high testosterone levels and the controls (p &gt; 0.05). Conclusions: Our findings especially regarding LH and T levels are not in agreement with previous reports. In this regard, there is a need for larger-scale and randomized trials to resolve this discrepancy.

scholarly journals Assessment of quality in specific fractions of Large White Yorkshire boar semen

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  
Keyword(s):  
Cold Shock ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Membrane Cholesterol ◽  
Large White ◽  
Progressive Motility ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Quality Assessments

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.

scholarly journals From Spermiogram to Bio-Functional Sperm Parameters: When and Why Request Them?

2020 ◽  
Vol 9 (2) ◽  
pp. 406 ◽  
Author(s):  
Rosita A. Condorelli ◽  
Aldo E. Calogero ◽  
Giorgio I. Russo ◽  
Sandro La Vignera
Keyword(s):  
Normal Form ◽  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Sperm Parameter ◽  
Progressive Motility ◽  
Semen Volume ◽  
Chromatin Compactness

The aim of this experimental study was to evaluate whether infertile patients may benefit from the evaluation of bio-functional sperm parameters in addition to the conventional semen analysis. To accomplish this, we evaluated the correlation between conventional and bio-functional sperm parameters based on their percentile distribution in search of a potential threshold of these latter that associates with conventional sperm parameter abnormalities. The study was conducted on 577 unselected patients with infertility lasting at least 12 months. We identified cut-off values according to the median of the population for mitochondrial membrane potential (MMP), number of alive spermatozoa, and chromatin abnormality. High MMP (HMMP) (≥46.25%) was associated with sperm concentration, sperm count, progressive motility, and normal form. Low MMP (LMMP) (≥36.5%) was found to be associated with semen volume, sperm concentration, total sperm count, progressive motility, total motility, and normal form. The number of alive spermatozoa (≥71.7%) was associated with sperm concentration and progressive motility whereas abnormal chromatin compactness (≥21.10%) was associated with sperm concentration, total sperm count, and progressive motility. The data would suggest that, for every increase in the percentile category of sperm concentration, the risk of finding an HMMP≤46.25 is reduced by 0.4 and by 0.66 for a total sperm count. This risk is also reduced by 0.60 for every increase in the percentile category of sperm progressive motility and by 0.71 for total sperm motility. Each increment of percentile category of the following sperm parameter was followed by a decrease in the risk of finding an LMMP≤36.5: sperm concentration 1.66, total sperm count 1.28, sperm progressive motility 1.27, total sperm motility 1.76, and normal form 1.73. Lastly, the data showed that, for every increase in the percentile category of total sperm count, the risk of finding an abnormal chromatin compactness ≤21.10 is reduced by 1.25 (1.04–1.51, p < 0.05) and an increase of total sperm motility is associated with a reduced risk by 1.44 (1.12–1.85, p < 0.05). Results suggest a correlation between bio-functional and conventional sperm parameters that impact the sperm fertilizing potential. Therefore, the evaluation of bio-functional sperm parameters by flow cytometry may be useful to explain some cases of idiopathic male infertility.

A COMPARISON OF CROSSBRED AND PUREBRED BOARS FOR SEMEN AND REPRODUCTIVE CHARACTERISTICS

1978 ◽  
Vol 58 (1) ◽  
pp. 63-70 ◽  
Author(s):  
P. D. CONLON ◽  
B. W. KENNEDY
Keyword(s):  
Reproductive Performance ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Reproductive Characteristics ◽  
Rate Score ◽  
Double Mating ◽  
Semen Volume ◽  
Semen Characteristics

Crossbred Hampshire–Duroc boars were compared to purebred Hampshire, Duroc and Landrace boars for seven semen characteristics and for reproductive performance under competitive double mating. A total of 14 boars were represented: three Hampshire–Duroc, three Hampshire, three Duroc and five Landrace. Significant breed of boar effects were observed on semen volume (P <.01), sperm concentration (P <.10), concentration score (P <.05), morphology score (P <.10), live–dead rate score (P <.05) and total semen score (P <.01). Landrace and Hampshire–Duroc boars produced more than twice the volume of semen produced by purebred Hampshires and Durocs. Hampshire, Duroc and Hampshire–Duroc boars had greater sperm concentrations and concentration scores than Landrace. Hampshire–Duroc boars ranked highest for morphology and total semen scores, but lowest for live–dead rate score. When double-mated to Landrace sows, breed of boar had no significant effect on conception rate but significantly affected (P <.05) percent of litter sired. Hampshire, Duroc and Hampshire–Duroc boars sired almost twice as many pigs as the Landrace.

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