Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ± to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.

Download Full-text

Comparative study on the semen quality characteristics of parthenotes and parent toms reared in Nsukka

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i5.288 ◽

2020 ◽

Vol 46 (5) ◽

Author(s):

F. U. Udeh ◽

I. J. Ohagenyi ◽

N. E. Ikeh ◽

M. O. Onodugo ◽

V. C. Udeh ◽

...

Keyword(s):

Sperm Cell ◽

Semen Quality ◽

Sperm Concentration ◽

Quality Characteristics ◽

Semen Parameters ◽

Motile Sperm ◽

Semen Collection ◽

Normal Sperm ◽

Abnormal Sperm ◽

Significant Difference

The study was conducted to evaluate the semen quality characteristics of turkey parthenotes and parent toms. A total of twenty-four turkeys were used for the study. These comprised of twelve parthenotes toms and twelve parent toms. Parthenotes are male turkeys produced through parthenogenesis. The toms were subjected to abdominal massage techniques for semen collection. Semen collection was done twice per week and analyzed for volume, motility, sperm concentration, like sperm, abnormal sperm, normal sperm, dead sperm, total sperm/ejaculates and total motile sperm cells. The results showed that there were significant differences (p<0.05) in semen volume, sperm concentration, normal sperm cell, abnormal sperm and total motile sperm with mean values being 0.35mL, 1.74x109/mL, 97.80%, 2.20%, 1.68x1012 and 0.41ml, 1.50x109/mL, 94.00%, 6.00% and 1.42x1012, respectively for both parthenotes and parent toms. However, semen parameters such as motility, live sperm cell, dead sperm cell and total sperm/ejaculate showed no significant difference (p>0.05) with values of 96.7%, 97.7%, 2.30% and 6.07x109 for parthenotes toms and 93.20%, 98.50%, 1.50% and 6.23x109 for parent toms. It was observed from the results that turkey parthenotes possess good quality semen characteristic and therefore, recommended for use in poultry industry for turkey production.

Download Full-text

Assessment of Semen Characteristics Among Three Phenotypes of Chicken Raised in Akko, Gombe State of Nigeria

Journal Of Agrobiotechnology ◽

10.37231/jab.2021.12.1.242 ◽

2021 ◽

Vol 12 (1) ◽

pp. 40-48

Author(s):

Tijjani Haruna Usman ◽

Saleh Mohammed Sir ◽

Sadiq Haladu ◽

Asmad Kari

Keyword(s):

Artificial Insemination ◽

Sperm Concentration ◽

Progressive Motility ◽

Sperm Abnormalities ◽

Normal Sperm ◽

Poultry Breeding ◽

Semen Volume ◽

Semen Characteristics ◽

Abdominal Massage

In poultry breeding program the success of artificial insemination (AI) is highly influenced by the quality of semen. The aim of the present research was to evaluate the variations in semen characteristics of three phenotypes of chicken of the same specie raised in Akko, Nigeria. A total of 9 cocks from threedifferent phenotypes; Red feathered (n = 3), White feathered (n = 3) and Black feathered (n =3). The semen was evaluated for macroscopic (i.e. semen volume and colour) and microscopic (i.e. sperm concentration, motility and morphology) criteria after being collected by abdominal massage method. There were phenotypes variation (P > 0.05)effects on semen motility, number of live/dead sperm and sperm abnormalities. No differences(P>0.05) were observed on volume, colour, concentration, mass motility and pH of semen. The observed sperm progressive motility was ranged from 90.5 ±1.21% to 95.09±0.82%. The White feathered strain had the highest sperm progressive motility (P<0.05)and highest value for live and normal sperm (93.5±0.63% and 87.90±0.25%), while the black feathered had the least and (92.4±0.73% and 85.5±0.50%) respectively. The Red feathered strain had the highest value for both dead and sperm head defect (9.2±0.33%and 7.0±0.52%). Whilst, the Black feathered strain scored the highest percentage of both sperm tail and neck defects with values ranged from 11.4±0.43%to 14.2±1.08% and 19.4±0.53% to 23.6±0.69%, respectively. This study suggests that there are large variations present in semen characteristics of different phenotypes of cocks; White feathered strain is likely have better semen characteristics compared to Red and Black feathered strains. Therefore, White feathered strain can potentially be used in artificial insemination (AI) for chicken production and improvement.

Download Full-text

116. EFFECTS OF SUPPLEMENTAL VITAMIN ON THE SEMEN CHARACTERISTICS OF MARKHOZ GOATS DURING NON-BREEDING SEASON

Reproduction Fertility and Development ◽

10.1071/srb09abs116 ◽

2009 ◽

Vol 21 (9) ◽

pp. 35

Author(s):

A. Towhidi ◽

A. Farshad ◽

M. Dolatpanah ◽

R. Salehi

Keyword(s):

Vitamin E ◽

Breeding Season ◽

Semen Quality ◽

Sperm Concentration ◽

Progressive Motility ◽

Semen Volume ◽

Semen Characteristics ◽

Supplemental Vitamin ◽

Diet Control ◽

Live Sperm

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.

Download Full-text

COMPARATIVE STUDIES OF THE REPRODUCTIVE CAPACITY OF NIGERIAN AND EXOTIC POULTRY BREEDS

Nigerian Journal of Animal Production ◽

10.51791/njap.v6i.2657 ◽

2021 ◽

Vol 6 ◽

pp. 47-51

Author(s):

G. N. Egbunike ◽

J. A. Oluyemi

Keyword(s):

Rhode Island ◽

Sperm Concentration ◽

Time Effect ◽

Reproductive Capacity ◽

Testis Weight ◽

White Leghorn ◽

Progressive Motility ◽

Indigenous Breed ◽

Sperm Abnormalities ◽

Semen Characteristics

THE study involved 48 adult cocks from four breeds namely, White Rock (WR), Rhode Island Red (RIR), White Leghorn (WL) and Nigerian indigenous breed (NIB). They weighed between 1 -26kg at the beginning of the experiment. The WR cock had the heaviest paired testis Weight (25.06g) while NIB had the lightest (16.549). These weights accounted for 1.04% and 1.22% of the liveweight respectively while the specific gravity of the testis from these two breeds was 1.048. The volume (0.63ml vs 0.33ml) and progressive motility of the spermatozoa (63.34 vs 58.12%) were highest in the WR and lowest in the NIB while sperm concentration (3.03 vt 2.43 x 10') was highest also in the WR but lowest in the WL. These semen characteristics were significantly influenced by breed and time of ejaculation except with the volume in which the time effect was Insignificant. The WR semen had the lowest sperm abnormalities (10.13% while RIR had the highest (13.14%). The results however Indicate that the WR cocks ejaculated the largest quantity and best quality semen, followed in order by RIR, NIB and WL

Download Full-text

80 Use of an Electronically-controlled Floor Cooling Pad During Heat Stress on Thermoregulatory and Reproductive Performance in Boars

Journal of Animal Science ◽

10.1093/jas/skab054.174 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 107-107

Author(s):

Larissa K Shirley ◽

Tyler Field ◽

Allan P Schinckel ◽

Jay S Johnson ◽

Robert Stwalley ◽

...

Keyword(s):

Heat Stress ◽

Semen Quality ◽

Sperm Concentration ◽

Controlled Cooling ◽

Ir Camera ◽

Progressive Motility ◽

Normal Sperm ◽

Semen Volume ◽

Negative Impacts ◽

Kinematic Motion

Abstract This study was designed to evaluate the effects of electronically-controlled floor cooling pads on thermoregulatory and reproductive parameters in boars during heat stress (HS). Boars (n = 24) were randomly assigned to crates with non-functional pads (CON) or pads that were flushed in either 8-min intervals or when the pad reached 28.5°C (FLUSH). For 3 d, boars were subjected to cyclical HS (28 to 35°C; >65% relative humidity). Boars were fed 2.4 kg/d and daily feed intake was recorded. Respiration rate (RR), rectal temperature (Rtemp) and skin temperature were recorded every 2 h during HS (via IR camera), testicular temperature was recorded twice daily. Semen was collected d7 and d14 before HS, the day following HS and weekly for 6 weeks and evaluated for volume, sperm concentration, motility, progressive motility, morphological abnormalities, and viability. After 2 h of HS, FLUSH boars had reduced RR (P < 0.001) and RTemp (P < 0.001) when compared to control boars, and this difference was maintained throughout HS. Skin and testicular temperature were reduced in FLUSH vs. CON boars after 6 h of HS (P < 0.05). Semen volume was greater in FLUSH vs CON boars (P = 0.01) resulting in a tendency for an increase in total sperm per ejaculate (P = 0.075). From weeks 2 to 5 post-HS, FLUSH boars had increased motility (P = 0.006) and progressive motility (P = 0.001), with corresponding increases in sperm kinematic motion parameters when compared to CON boars. The number of morphologically normal sperm cells were increased (P = 0.006) in FLUSH vs CON boars due to reduced distal droplets (P = 0.033) and proximal droplets (P < 0.001). Abnormal acrosomes were reduced (P < 0.001) in FLUSH vs CON boars at week 3 post-HS. In summary, electronically controlled cooling pads effectively reduced negative thermoregulatory indicators of HS and minimized or removed the negative impacts of HS on semen quality in boars.

Download Full-text

The relationship between serum hormone levels (follicle-stimulating hormone, luteinizing hormone, total testosterone) and semen parameters

Archivio Italiano di Urologia e Andrologia ◽

10.4081/aiua.2015.3.194 ◽

2015 ◽

Vol 87 (3) ◽

pp. 194 ◽

Cited By ~ 8

Author(s):

Mehmet Zeynel Keskin ◽

Salih Budak ◽

Tuǧba Zeyrek ◽

Orçun Çelik ◽

Oguz Mertoglu ◽

...

Keyword(s):

Sperm Concentration ◽

Reference Value ◽

Semen Parameters ◽

Total Testosterone ◽

Testosterone Levels ◽

Progressive Motility ◽

Semen Volume ◽

Significant Difference ◽

Serum Hormone ◽

Serum Gonadotropin

Objective: The aim of this study was to investigate the effect of serum gonadotropin and total testosterone levels on semen parameters. Materials and Methods: Three hundred and eighty-two patients that applied to a male infertility polyclinic were included in our study. Serum gonadotropin and total testosterone levels and semen parameters of the patients were analyzed during the first visit to the clinic. The reference FSH value was 1.5-12.4 mIU/mL, that of LH was 1.7-8.6 mIU/mL and the reference value for total testosterone was 249-836 ng/dL. Results: While there was no statistically significant difference between the patients with low gonadotropin levels and the controls regarding any of the semen parameters (p > 0.05), there was a strong statistically significant difference between the patients with high gonadotropin levels and the controls regarding sperm concentration (p = 0.000), total motility (p = 0.000), progressive motility (p = 0.000), and morphology (p = 0.000). There was a strong statistically significant difference between the patients with low testosterone levels and the controls regarding total motility (p = 0.012) and progressive motility (p = 0.010), and a weak statistically significant difference in morphology (p = 0.042). There was no statistically significant difference in semen volume or sperm concentration (p > 0.05). There was no statistically significant difference in any of the semen parameters between the patients with high testosterone levels and the controls (p > 0.05). Conclusions: Our findings especially regarding LH and T levels are not in agreement with previous reports. In this regard, there is a need for larger-scale and randomized trials to resolve this discrepancy.

Download Full-text

Male traits and herd reproductive capability in tropical beef cattle. 2. Genetic parameters of bull traits

Animal Production Science ◽

10.1071/an12163 ◽

2013 ◽

Vol 53 (2) ◽

pp. 101 ◽

Cited By ~ 32

Author(s):

N. J. Corbet ◽

B. M. Burns ◽

D. J. Johnston ◽

M. L. Wolcott ◽

D. H. Corbet ◽

...

Keyword(s):

Body Condition ◽

Semen Quality ◽

Body Condition Score ◽

Progressive Motility ◽

Scrotal Circumference ◽

Normal Sperm ◽

Igf I ◽

Condition Score ◽

Mass Activity ◽

Percent Normal

A total of 4063 young bulls of two tropical genotypes (1639 Brahman and 2424 Tropical Composite) raised in northern Australia were evaluated for a comprehensive range of production and reproduction traits up to 24 months of age. Prior to weaning, peripheral blood concentrations of luteinising hormone (LH) and inhibin were measured at 4 months of age. At weaning (6 months) blood insulin-like growth factor-1 (IGF-I) and flight time were recorded. Body composition traits of fat depth and eye-muscle area were determined by ultrasonography at 15 months of age when additional measurements of liveweight, hip height and body condition score were recorded. Bull breeding soundness was evaluated at ~12, 18 and 24 months of age when measurements of scrotal circumference, sheath score, semen mass activity, progressive motility of individual sperm and percent morphologically normal sperm were recorded. Magnitude of heritability and genetic correlations changed across time for some traits. Heritability of LH, inhibin, IGF-I and of 18-month scrotal circumference, mass activity, progressive motility and percent normal sperm was 0.31, 0.74, 0.44, 0.75, 0.24, 0.15 and 0.25, respectively, for Brahmans and 0.48, 0.72, 0.36, 0.43, 0.13, 0.15 and 0.20, respectively, for Tropical Composites. Inhibin and IGF-I had moderate genetic association with percent normal sperm at 24 months in Brahmans but low to negligible associations in Tropical Composites. Body condition score in Brahmans and sperm motility (mass and individual) traits in both genotypes had moderate to strong genetic correlation with percent normal sperm and may prove useful candidates for indirect selection. There is scope to increase scrotal circumference by selection and this will be associated with favourable correlated responses of improved semen quality in both genotypes. The lack of genetic antagonism among bull traits indicates that selection for improved semen quality will not adversely affect other production traits.

Download Full-text

Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v7i1.23312 ◽

2019 ◽

Vol 7 (1) ◽

pp. 88-95 ◽

Cited By ~ 2

Author(s):

S. Sankhi ◽

K.R. Sapkota ◽

B. Regmi

Keyword(s):

Semen Quality ◽

Sperm Concentration ◽

Quality Parameters ◽

Bull Semen ◽

Livestock Breeding ◽

Abnormal Sperm ◽

Breeding Center ◽

Live Sperm ◽

Level Of Significance ◽

Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

Download Full-text

The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, Manis javanica

Animals ◽

10.3390/ani10111948 ◽

2020 ◽

Vol 10 (11) ◽

pp. 1948

Author(s):

Reza Tarmizi ◽

Yap Keng Chee ◽

Symphorosa Sipangkui ◽

Zainal Zahari Zainuddin ◽

Wan-Nor Fitri

Keyword(s):

Standard Deviation ◽

Progressive Motility ◽

Semen Collection ◽

Significant Difference ◽

Semen Characteristics ◽

Manis Javanica ◽

Consistent Method ◽

The Mean ◽

Genetic Pool ◽

Collection Methods

This article describes the semen characteristics from different collection methods between captive and confiscated Malayan pangolins, Manis javanica. Semen was collected from 15 pangolins; two captive and 13 confiscated individuals at the mean weight of 9.36 ± 1.94 kg. The three semen collection methods employed were electroejaculation, rectal massage and a combination of both techniques. The semen characteristics (mean ± standard deviation) of the Malayan pangolin are volume (73.75 ± 144.57 µL), pH (7.63 ± 0.53), spermatozoa concentration (997.19 ± 728.98 × 106 /mL), total motility (59.60% ± 30.00%), progressive motility (48.95% ± 30.93%), mass motility (3.50 ± 1.50) and live spermatozoa (80.25% ± 13.45%). There was no significant difference in semen characteristics between the three collection methods. The percentages of live spermatozoa were significantly different, suggesting better samples from captive compared to confiscated animals. However, there was no significant difference in spermatozoa kinetics between the captive and confiscated samples, suggesting the potential of utilizing confiscated individuals for gamete recovery to conserve the genetic pool of pangolins. All three methods of semen collection were successfully performed in pangolins and should be considered; however, electroejaculation remains the most consistent method of obtaining semen from the species.

Download Full-text

Semen quality pattern and age threshold: a retrospective cross-sectional study of 71,623 infertile men in China, between 2011 and 2017

Reproductive Biology and Endocrinology ◽

10.1186/s12958-019-0551-2 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

W. N. Li ◽

M. M. Jia ◽

Y. Q. Peng ◽

R. Ding ◽

L. Q. Fan ◽

...

Keyword(s):

Semen Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Cross Sectional Study ◽

Semen Parameters ◽

Sectional Study ◽

Joinpoint Regression ◽

Cross Sectional ◽

Progressive Motility ◽

Infertile Men

Abstract Background The aim of this study was to provide information on the semen quality pattern of infertile men and age thresholds for semen parameters in China. Methods This was a retrospective cross-sectional study investigating 71,623 infertile men from the Reproductive and Genetic Hospital of CITIC Xiangya in Hunan, China, from 2011 to 2017. The Kruskal-Wallis test, Mann-Kendall test, linear regression model and joinpoint regression were used. Results Although erratic changes were observed in the median semen parameters (sperm concentration 40.1–52.1 × 106/ml, total sperm count 117.8–153.1 × 106, sperm progressive motility 33.4–38.1%) during the 7 years of observation, no significant decrease in semen quality was found, and 47.88% of infertile men showed normal semen parameters according to the World Health Organization (WHO) criteria. According to the joinpoint regression analysis, sperm progressive motility appeared to decrease earlier than the sperm concentration and total sperm count (at 28, 58, and 42 years of age, respectively). Conclusions There is no evidence of a deterioration in semen quality among infertile men in Hunan, China. Semen parameters decreased with increasing age, with turning points noted at different ages. Semen parameters are not absolute evidence for the assessment of male fertility potential. Therefore, we believe that, among semen parameters, the sperm concentration is the best predictor of fertility for ART, followed by motility. Decreased sperm motility may affect natural pregnancy, but it is not necessary for successful IVF.

Download Full-text