Cloning and function identification of gene �?admA�? and up-stream regulatory sequence related to antagonistic activity of Enterobacter cloacae B8

2012 ◽  
Vol 34 (4) ◽  
pp. 495-502
Author(s):  
Jun-Li ZHU ◽  
De-Bao LI ◽  
Xu-Ping YU
Keyword(s):  
Enterobacter Cloacae ◽  
Antagonistic Activity ◽  
Regulatory Sequence ◽  
Function Identification ◽  
And Function

The Preparation and Function Identification of a Novel Anti-Fumarate Hydratase Antibody

2013 ◽  
Vol 29 (1) ◽  
pp. 46
Author(s):  
Meihong FU ◽  
Liang LE ◽  
Zhong CHENG ◽  
Yong XIE ◽  
Hai GAO ◽  
...  
Keyword(s):  
Fumarate Hydratase ◽  
Function Identification ◽  
And Function

scholarly journals Supervillin modulation of focal adhesions involving TRIP6/ZRP-1

2006 ◽  
Vol 174 (3) ◽  
pp. 447-458 ◽  
Author(s):  
Norio Takizawa ◽  
Tara C. Smith ◽  
Thomas Nebl ◽  
Jessica L. Crowley ◽  
Stephen J. Palmieri ◽  
...  
Keyword(s):  
Focal Adhesions ◽  
Stress Fibers ◽  
Regulatory Sequence ◽  
Interacting Protein ◽  
Substrate Adhesion ◽  
Lim Domains ◽  
Cell Substrate ◽  
Thyroid Receptor ◽  
And Function ◽  
Cell Substrate Adhesion

Cell–substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that supervillin (SV)—a peripheral membrane protein that binds myosin II and F-actin in such cells—negatively regulates stress fibers, FAs, and cell–substrate adhesion. The major FA regulatory sequence within SV (SV342-571) binds to the LIM domains of two proteins in the zyxin family, thyroid receptor–interacting protein 6 (TRIP6) and lipoma-preferred partner (LPP), but not to zyxin itself. SV and TRIP6 colocalize within large FAs, where TRIP6 may help recruit SV. RNAi-mediated decreases in either protein increase cell adhesion to fibronectin. TRIP6 partially rescues SV effects on stress fibers and FAs, apparently by mislocating SV away from FAs. Thus, SV interactions with TRIP6 at FAs promote loss of FA structure and function. SV and TRIP6 binding partners suggest several specific mechanisms through which the SV–TRIP6 interaction may regulate FA maturation and/or disassembly.

Definition and function identification of nucleus export signal of SGIV ICP46

2012 ◽  
Vol 36 (11) ◽  
pp. 1763
Author(s):  
Li-qun XIA ◽  
Hong-lian ZHANG ◽  
Qi-wei QIN
Keyword(s):  
Function Identification ◽  
And Function ◽  
Export Signal

scholarly journals Functional anatomy of pelvic floor

2016 ◽  
Vol 88 (1) ◽  
pp. 28 ◽  
Author(s):  
Salvatore Rocca Rossetti
Keyword(s):  
Pelvic Floor ◽  
Functional Anatomy ◽  
Body Part ◽  
Antagonistic Activity ◽  
Whole Body ◽  
Statics And Dynamics ◽  
Intestinal Functions ◽  
Global Aspect ◽  
And Function ◽  
And Behavior

Generally, descriptions of the pelvic floor are discordant, since its complex structures and the complexity of pathological disorders of such structures; commonly the descriptions are sectorial, concerning muscles, fascial developments, ligaments and so on. On the contrary to understand completely nature and function of the pelvic floor it is necessary to study it in the most unitary view and in the most global aspect, considering embriology, philogenesy, anthropologic development and its multiple activities others than urological, gynaecological and intestinal ones. Recent acquirements succeeded in clarifying many aspects of pelvic floor activity, whose musculature has been investigated through electromyography, sonography, magnetic resonance, histology, histochemistry, molecular research. Utilizing recent research concerning not only urinary and gynecologic aspects but also those regarding statics and dynamics of pelvis and its floor, it is now possible to study this important body part as a unit; that means to consider it in the whole body economy to which maintaining upright position, walking and behavior or physical conduct do not share less than urinary, genital, and intestinal functions. It is today possible to consider the pelvic floor as a musclefascial unit with synergic and antagonistic activity of muscular bundles, among them more or less interlaced, with multiple functions and not only the function of pelvic cup closure.

scholarly journals The Ets-1 transcription factor controls the development and function of natural regulatory T cells

2010 ◽  
Vol 207 (10) ◽  
pp. 2113-2125 ◽  
Author(s):  
Enguerran Mouly ◽  
Karine Chemin ◽  
Hai Vu Nguyen ◽  
Martine Chopin ◽  
Laurent Mesnard ◽  
...  
Keyword(s):  
T Cells ◽  
Transcription Factor ◽  
T Cell ◽  
Regulatory T Cells ◽  
Molecular Mechanisms ◽  
Fetal Liver ◽  
Regulatory Sequence ◽  
Activated T Cells ◽  
And Function

Regulatory T cells (T reg cells) constitute a population of CD4+ T cells that limits immune responses. The transcription factor Foxp3 is important for determining the development and function of T reg cells; however, the molecular mechanisms that trigger and maintain its expression remain incompletely understood. In this study, we show that mice deficient for the Ets-1 transcription factor (Ets-1−/−) developed T cell–mediated splenomegaly and systemic autoimmunity that can be blocked by functional wild-type T reg cells. Spleens of Ets-1−/− mice contained mostly activated T cells, including Th2-polarized CD4+ cells and had reduced percentages of T reg cells. Splenic and thymic Ets-1−/− T reg cells expressed low levels of Foxp3 and displayed the CD103 marker that characterizes antigen-experienced T reg cells. Thymic development of Ets-1−/− T reg cells appeared intrinsically altered as Foxp3-expressing cells differentiate poorly in mixed fetal liver reconstituted chimera and fetal thymic organ culture. Ets-1−/− T reg cells showed decreased in vitro suppression activity and did not protect Rag2−/− hosts from naive T cell–induced inflammatory bowel disease. Furthermore, in T reg cells, Ets-1 interacted with the Foxp3 intronic enhancer and was required for demethylation of this regulatory sequence. These data demonstrate that Ets-1 is required for the development of natural T reg cells and suggest a role for this transcription factor in the regulation of Foxp3 expression.

scholarly journals Evolution of cis-regulatory sequence and function in Diptera

Heredity ◽  
2006 ◽  
Vol 97 (3) ◽  
pp. 139-147 ◽  
Author(s):  
P J Wittkopp
Keyword(s):  
Regulatory Sequence ◽  
And Function

scholarly journals A Disease-associated Polymorphism Alters Splicing of the Human CD45 Phosphatase Gene by Disrupting Combinatorial Repression by Heterogeneous Nuclear Ribonucleoproteins (hnRNPs)

2011 ◽  
Vol 286 (22) ◽  
pp. 20043-20053 ◽  
Author(s):  
Laura B. Motta-Mena ◽  
Sarah A. Smith ◽  
Michael J. Mallory ◽  
Jason Jackson ◽  
Jiarong Wang ◽  
...  
Keyword(s):  
T Cell Activation ◽  
Cell Activation ◽  
Mutational Analysis ◽  
Regulatory Proteins ◽  
Regulatory Function ◽  
Regulatory Sequence ◽  
Splicing Regulation ◽  
Sequence Motifs ◽  
Core Motif ◽  
And Function

Alternative splicing is typically controlled by complexes of regulatory proteins that bind to sequences within or flanking variable exons. The identification of regulatory sequence motifs and the characterization of sequence motifs bound by splicing regulatory proteins have been essential to predicting splicing regulation. The activation-responsive sequence (ARS) motif has previously been identified in several exons that undergo changes in splicing upon T cell activation. hnRNP L binds to this ARS motif and regulates ARS-containing exons; however, hnRNP L does not function alone. Interestingly, the proteins that bind together with hnRNP L differ for different exons that contain the ARS core motif. Here we undertake a systematic mutational analysis of the best characterized context of the ARS motif, namely the ESS1 sequence from CD45 exon 4, to understand the determinants of binding specificity among the components of the ESS1 regulatory complex and the relationship between protein binding and function. We demonstrate that different mutations within the ARS motif affect specific aspects of regulatory function and disrupt the binding of distinct proteins. Most notably, we demonstrate that the C77G polymorphism, which correlates with autoimmune disease susceptibility in humans, disrupts exon silencing by preventing the redundant activity of hnRNPs K and E2 to compensate for the weakened function of hnRNP L. Therefore, these studies provide an important example of the functional relevance of combinatorial function in splicing regulation and suggest that additional polymorphisms may similarly disrupt function of the ESS1 silencer.

Facilitated Expression and Function Identification of Key Genes Converting Cyanocobalamin to Adenosylcobalamin

2012 ◽  
Vol 18 (2) ◽  
pp. 267
Author(s):  
Weifeng ZHAI ◽  
Huiying FANG ◽  
Bin ZHUGE ◽  
Cheng ZHANG ◽  
Yingmin XUE ◽  
...  
Keyword(s):  
Key Genes ◽  
Function Identification ◽  
And Function
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