Isolation of the Promoter Region of a Pollen-specific Gene PSG076 by Inverse-PCR

Tissue and organ-specific expression genes induced by Cis-regulator elements (CREs) are distributed in the promoter region of a gene. In rice, pollen-specific genes have been investigated to identify CREs related to specific expression in anther and pollen grain such as GTGANTG10, POLLEN1LELAT52... RMP1 and RMP2genes that specifically express the early stage of pollen development were analysed their promoter region using NEW PLACE and PlantCARE tools. Results showed that 80 CREs were located in the RMP1 promoter and 95 CREs in the RMP2. Among them, 6 CREs are abundant distribution with from 12 to 25 copies, including ARR1AT, CAATBOX1, CACTFTPPCA1, DOFCOREZM, EBOXBNNAPA, and GATABOX. Interestingly, two pollen-specific CREs, GTGANTG10, POLLEN1LELAT52, were also found in RMP1 and RMP2 promoters. In which, GTGANTG10 was counted 14 copies in RMP1 and 21 copies in RMP2, whereas, POLLEN1LELAT52 was found 4 and 21 copies in RMP1and RMP2, respectively

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Identification of the mouse neurochondrin promoter region and the responsible region for cell type specific gene regulation

Neuroscience Letters ◽

10.1016/j.neulet.2003.11.026 ◽

2004 ◽

Vol 356 (2) ◽

pp. 107-110 ◽

Cited By ~ 3

Author(s):

Minori Dateki ◽

Reiko Mochizuki ◽

Kazuyuki Yanai ◽

Akiyoshi Fukamizu

Keyword(s):

Gene Regulation ◽

Promoter Region ◽

Specific Gene ◽

Cell Type ◽

Cell Type Specific

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A revised sequence of the human β7 integrin gene ( ITGB7 ) promoter region obtained by inverse PCR

Immunogenetics ◽

10.1007/s002510000218 ◽

2000 ◽

Vol 51 (10) ◽

pp. 863-865 ◽

Cited By ~ 2

Author(s):

David A. van Heel ◽

Maxine Allen ◽

Derek P. Jewell ◽

Alisoun H. Carey

Keyword(s):

Promoter Region ◽

Inverse Pcr ◽

Integrin Gene

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Cloning and analysis of the promoter region of the rat SM22α gene

Biochemical Journal ◽

10.1042/bj3101037 ◽

1995 ◽

Vol 310 (3) ◽

pp. 1037-1043 ◽

Cited By ~ 30

Author(s):

P R Kemp ◽

J K Osbourn ◽

D J Grainger ◽

J C Metcalfe

Keyword(s):

Smooth Muscle ◽

Smooth Muscle Cells ◽

Promoter Region ◽

Core Promoter ◽

Muscle Cells ◽

Cell Types ◽

Specific Gene ◽

Upstream Sequence ◽

Carg Box ◽

Alpha Actin

We have cloned and sequenced a 1.9 kb fragment of the 5′-upstream sequence of the smooth-muscle-specific gene SM22 alpha. The region cloned consisted of the SM22 alpha promoter, a 65 bp exon containing most of the 5′-untranslated region and 307 bp of the first intron. A 1.5 kb fragment at the 5′ end of this sequence was able to drive the expression of a reporter chloramphenicol acetyltransferase (CAT) gene in both vascular smooth-muscle cells and Rat-1 fibroblasts. This promoter region did not contain a consensus TATAA box but contained the sequence TTTAAA 25 bp from the major start site identified by primer extension. Deletion analysis showed that a fragment of the promoter from +65 to -303 was more active in both cell types than the 1.5 kb fragment suggesting that there are silencer sequences in the region 5′ to the core promoter. CAT activity was also observed with fragments containing bases +65 to -193 and +65 to -117 in smooth-muscle cells. In contrast with the smooth-muscle cells, no CAT activity was detected in Rat-1 fibroblasts with the smallest two fragments. The residual promoter activity in the smallest fragment of the SM22 alpha promoter tested suggested that, unlike the smooth-muscle alpha-actin promoter, transcription from the SM22 alpha promoter can occur in smooth-muscle cells in the absence of factors binding to CC(A/Trich)6GG (CArG box) or CANNTG (E box) motifs.

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DNA Methylation Regulates Placental Lactogen I Gene Expression

Endocrinology ◽

10.1210/endo.142.8.8347 ◽

2001 ◽

Vol 142 (8) ◽

pp. 3389-3396 ◽

Cited By ~ 29

Author(s):

Jae-Hyeon Cho ◽

Hiromichi Kimura ◽

Tatsuya Minami ◽

Jun Ohgane ◽

Naka Hattori ◽

...

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Promoter Region ◽

Trichostatin A ◽

Methylation Status ◽

Placental Lactogen ◽

Specific Gene ◽

Tissue Specific ◽

Reporter Activity ◽

I Gene

Abstract Expression of rat placental lactogen I is specific to the placenta and never expressed in other tissues. To obtain insight into the mechanism of tissue-specific gene expression, we investigated the methylation status in 3.4 kb of the 5′-flanking region of the rat placental lactogen I gene. We found that the distal promoter region of the rat placental lactogen I gene had more potent promoter activity than that of the proximal area alone, which contains several possible cis-elements. Although there are only 17 CpGs in the promoter region, in vitro methylation of the reporter constructs caused severe suppression of reporter activity, and CpG sites in the placenta were more hypomethylated than other tissues. Coexpression of methyl-CpG-binding protein with reporter constructs elicited further suppression of the reporter activity, whereas treatment with trichostatin A, an inhibitor of histone deacetylase, reversed the suppression caused by methylation. Furthermore, treatment of rat placental lactogen I nonexpressing BRL cells with 5-aza-2′-deoxycytidine, an inhibitor of DNA methylation, or trichostatin A resulted in the de novo expression of rat placental lactogen I. These results provide evidence that change in DNA methylation is the fundamental mechanism regulating the tissue-specific expression of the rat placental lactogen I gene.

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