This book explains how a conspiracy trial featuring English, Japanese, and Indo-Portuguese co-conspirators who allegedly plotted against the Dutch East India Company in the Indian Ocean in 1623 produced a diplomatic crisis in Europe and became known for four centuries in British culture as the Amboyna Massacre. The story of the transformation of this conspiracy into a massacre is a story of Anglo-Dutch relations in the seventeenth century and of a new word in the English language, massacre. The English East India Company drew on this new word to craft an enduring story of cruelty, violence, and ingratitude. Printed works—both pamphlets and images—were central to the East India Company’s creation of the massacre and to the story’s tenacity over four centuries as the texts and images were reproduced during conflicts with the Dutch and internal political disputes in England. By the eighteenth century, the story emerged as a familiar and shared cultural touchstone. By the nineteenth century, the Amboyna Massacre became the linchpin of the British Empire, an event that historians argued well into the twentieth century had changed the course of history and explained why the British had a stronghold in India. The broad familiarity with the incident and the Amboyna Massacre’s position as an early and formative violent event turned the episode into the first English massacre. It shaped the meaning of subsequent acts of violence, and placed intimacy, treachery, and cruelty at the center of massacres in ways that endure to the present day.