scholarly journals Islet Cryopreservation: Improved Recovery following Taurine Pretreatment

2001 ◽  
Vol 10 (3) ◽  
pp. 247-253 ◽  
Author(s):  
Anandwardhan A. Hardikar ◽  
Makarand V. Risbud ◽  
Claude Remacle ◽  
Brigitte Reusens ◽  
Joseph J. Hoet ◽  
...  
Keyword(s):  
Islet Cells ◽  
Radical Scavenging ◽  
The Body ◽  
Control Group ◽  
Image Analysis System ◽  
Islet Mass ◽  
Normal Glucose ◽  
Ethanesulfonic Acid ◽  
Analysis System ◽  
Digital Image Analysis System

Simple and efficient freezing methods with maximal postthawing recovery form the basis of ideal cryopreservation. Taurine (2-amino ethanesulfonic acid), an end-product of sulphur amino acid metabolism, is one of the most abundant free amino acids in the body. The membrane stabilizing, free radical scavenging, and osmoregulatory roles of taurine have been well documented. We studied the effect of physiological and supra-physiological concentrations (0.3 and 3.0 mM) of taurine on islet cryopreservation. Islet viability on cryopreservation was significantly improved in both the taurine-treated groups (91.9 ± 2.3% in 0.3 mM and 94.6 ± 1.58% in 3.0 mM group, p < 0.05) compared with the controls (85.7 ± 3.4%). Loss of peripheral islet cells was highly reduced in the taurine group, as examined under phase contrast and quantified by islet morphometric analysis (p < 0.05) using a digital image analysis system. Taurine-treated islets showed significant reduction in lipid peroxidation (0.905 and 0.848 nM MDA/μg protein for 0.3 and 3.0 mM taurine, respectively, p < 0.05) compared with control (1.307 nM MDA/μg protein) islets. In all, 500 islet equivalents (IE) of treated or control group islets were transplanted to BALB/c mice rendered diabetic with STZ. All animals showed a normal glucose clearance following a glucose load. Graft functionality was confirmed by normoglycemia (fasting plasma glucose: fpg < 150 mg/dl) after transplantation and reappearing hyperglycemia (fpg > 200 mg/dl) following removal of the graft. Suboptimal islet transplantation using 250 IE suggests that the grafted islet mass was inadequate for diabetes reversal. In addition, no significant differences were observed in the islet insulin content between the three groups following cryopreservation of the islets at −196°C. Our studies indicate that taurine pretreatment and its continued presence during islet cryopreservation improves the postthawing viable recovery of islets.

scholarly journals Histologic study of use of microfibrillar collagen hemostat in rat dental sockets

2003 ◽  
Vol 14 (1) ◽  
pp. 12-15 ◽  
Author(s):  
Natasha Magro-Érnica ◽  
Osvaldo Magro-Filho ◽  
Idelmo Rangel-Garcia
Keyword(s):  
Control Group ◽  
Image Analysis System ◽  
Bone Area ◽  
Central Incisor ◽  
Histologic Study ◽  
Socket Healing ◽  
Hematoxylin And Eosin ◽  
Analysis System ◽  
The Right ◽  
Qualitative Analyses

The aim of this paper was to evaluate if the placement of microfibrillar collagen hemostat (MCH) into a dental socket interfered with healing. General anesthesia was administered to 30 adult male Albinus Wistar rats and the maxillary right central incisor was extracted. In the control group after each tooth was extracted, the socket was sutured. In the MCH group after each tooth was extracted, MCH was placed into the socket before suturing. Postoperatively, 5 animals were sacrificed from each group at 7, 21 and 28 days. The right maxilla was removed from each animal and histologic slides were stained with Masson's trichromic and hematoxylin and eosin. Quantitative and qualitative analyses were done. The percentage of bone area in the dental socket was quantified using the Image Lab 98 image analysis system. The bone area formation for the control and MCH groups was: 8.1% and 3.3% at 7 days, 34.4% and 33% at 21 days and 41% and 41.3% at 28 days, respectively. We concluded that MCH interferes with the beginning of dental socket healing but does not interfere with the final healing of the dental socket.

Quantitation of the changes in vascularity during arthritis in the knee joint of a mouse with a digital image analysis system

2001 ◽  
Vol 262 (4) ◽  
pp. 420-428 ◽  
Author(s):  
Pieter Buma ◽  
Martin Groenenberg ◽  
Paul F.J.W. Rijken ◽  
Wim B. Van Den Berg ◽  
Leo Joosten ◽  
...  
Keyword(s):  
Image Analysis ◽  
Knee Joint ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Image Analysis System ◽  
Analysis System ◽  
Digital Image Analysis System

Study on the Regulation of Compound siRNA Nanoparticles on the Rat Model of Kidney Injury Induced by Sepsis by Inhibiting the Expression of NF-κB and P65

2021 ◽  
Vol 21 (2) ◽  
pp. 1345-1350
Author(s):  
Ye Chen ◽  
Xiaoxia Liu ◽  
Meiling Chen ◽  
Run Yan ◽  
Wenyu Song
Keyword(s):  
Rat Model ◽  
Group Treatment ◽  
Medical Image Analysis ◽  
Intervention Group ◽  
Kidney Tissue ◽  
Kidney Injury ◽  
Control Group ◽  
Image Analysis System ◽  
Model Group ◽  
Analysis System

This article explores the pathogenesis of sepsis AKI, and seeks to protect the acute damage of sepsis tissues and organs. This study is to prepare a rat sepsis-induced AKI model by CLP, and to observe the pathological changes of kidney tissue and the function of kidney changes, and observe the effect of siRNA nanoparticles on its intervention, preliminary explore the protective effect and possible mechanism of siRNA nanoparticles on AKI in sepsis rats, and provide more information for the clinical treatment of siRNA nanoparticles in sepsis theoretical and experimental basis. We analysis the benefit and deficiency of nuclear factor-κB (NF-κB) activation in the pathogenesis of glomerulonephritis and its regulatory effect on NF-κB activation. In the rat model group, no treatment was given after injection of nephrotoxic serum, and the rats were sacrificed on the 14th day; the compound siRNA nanoparticle intervention group (treatment group) was given dexamethasone 0.125 daily on the 1st to 14th day after nephrotoxic serum injection. Immunohistochemistry and medical image analysis system were used to observe NF-κB activation of monocyte chemotactic protein-1 (MCP-1) in glomeruli and tubules, and analyze their relationship with proteinuria and glomerular cells. The results showed that the expression of NF-κB in the glomeruli and tubules of the model group was significantly up-regulated regarding to the control group, and MCP-1’s expression in the glomeruli and tubules of the model group was higher than that of the control group. The activation of NF-κB and the expression of MCP-1 in glomeruli are closely related to monocyte infiltration and proteinuria; NF-κB activation and MCP-1 expression in glomeruli and tubules of the compound siRNA nanoparticles intervention group were significantly down-regulated. It was concluded that the activation of NF-κB has great impact on the pathogenesis of glomerulonephritis, and inhibition of NF-κB activation may be one of the mechanisms of anti-nephritis effect.

Numerical simulation of mold filling and particulate flow of A356/SiCp indirect squeeze casting

2016 ◽  
Vol 54 (12) ◽  
pp. 1593-1602
Author(s):  
Qiyao Hu ◽  
Haidong Zhao ◽  
Han Long ◽  
Puyun Dong ◽  
Gang Zhu
Keyword(s):  
Distribution Patterns ◽  
Mold Filling ◽  
Particulate Flow ◽  
Image Analysis System ◽  
Matrix Composites ◽  
Analysis System ◽  
Particulate Distribution ◽  
Particulate Reinforced ◽  
Acceptable Agreement ◽  
Digital Image Analysis System

In this investigation, a mathematical model based on the dispersed phase model and multiphase flow principle was introduced, and applied to simulate the mold filling and particulate flow of particulate reinforced metal matrix composites casting. Experiments of spiral-square shaped indirect squeeze castings of A356/50 µm SiCp were conducted to validate the established model. The SiCp fractions in the casting different locations were quantitatively measured with micro digital image analysis system and were compared with the simulation results. It was found that particulate fractions and distribution patterns at different locations were quite different along the filling distance. The experimental and simulated results about the SiCp distribution in the castings show acceptable agreement. Further, the effect of fluid flow and particulate trajectories on the particulate distribution was analyzed and discussed. The research has shown the validity of application of the model for practical particulate reinforced Al-based composites casting.

Model-based frequency response characterization of a digital-image analysis system for epifluorescence microscopy

1992 ◽  
Vol 31 (8) ◽  
pp. 1083
Author(s):  
Rajeeb Hazra ◽  
Charles L. Viles ◽  
Stephen K. Park ◽  
Stephen E. Reichenbach ◽  
Michael E. Sieracki
Keyword(s):  
Image Analysis ◽  
Frequency Response ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Epifluorescence Microscopy ◽  
Image Analysis System ◽  
Model Based ◽  
Analysis System ◽  
Digital Image Analysis System
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